猕猴正常月经周期血清甾体激素的测定

本研究对福建产猕猴的31个正常月经周期的血清雌二醇(E_2)、孕酮(P)进行放免测定。结果:E_2第一峰前x 122.37±49.86pg/ml;第一峰值372.06±168.21 pg/ml;黄体期77.21±34.10 pg/ml;有5个周期出现黄体期第二峰值279.91±97.46 pg/ml(P<0.01)。P滤泡期x 0.71±0.46 ng/ml;黄体期峰值6.047±3.307ng/ml,黄体期x 2.858±1.74 ng/ml。各测定数值均有较大个体差异。雌性猕猴月经周期平均为25.5±4.2天,与妇女平均28天相近。据有关资料描述,猴与妇女外周血E_2和P分泌图型相似。明显不同之处是,猴只在少数周期中观察到黄体期E_2分泌高峰,且该两种激素测定数值均低于妇女。     

唾液雌二醇、孕酮的放射免疫测定

本文采用氚标记放免测定法及内酮一干冰法分离抽提液测定了唾液雌二醇(E_2)和孕酮(P)浓度,经抗血清最佳滴度试验表明,抗E_2血清和抗P血清的最佳滴度均为1∶80,000,唾液E_2和P的测定灵敏度分别达3pg和3.5pg,非特异性结合率均为2.3％,外加标准回收率分别为96.7％和97.8％,批内变异系数(CV)分别为7.3％和7.2％,批间CV为13.8％和11.7％。30名成年男子唾液E_2浓度为1.8±1.6pg/ml(M±SD),是血清E_2浓度的2.1％;P浓度为50.3±25.0Pg/ml,是血清P浓度的9.4％。26名有排卵月经的妇女唾液E_2浓度在卵泡早期和黄体期各为4.5±2.0和5.8±2.8pg/ml,P浓度各为53.1±26.1和126.3±76.7Pg/ml,其中3人在一个月经周期内同时隔天测唾液和血清的E_2和P,显示两种体液中激素浓度呈平行变化。     

武汉地区正常育龄妇女月经周期血清中七种激素的放射免疫测定

选抒12例正常月经周期的武汉地区妇女,研究她们的内分泌图象,采用放射免疫测定法测定每天血清中促黄体生成激素(LH)、促卵泡生成素(FSH)、垂体催乳素(PRL)、雌二醇(E_2)、孕酮(P)、睾丸酮(T)和皮质激素(C)的浓度。其中LH、FSH、E_2和P的周期变化和水平与其他文献报告的结果相同,月经中期LH峰值是50mIU/ml,FSH峰值为12mIU/ml,排卵前E_2峰值为252pg/ml,P值为16ng/ml,在大多数周期中,PRL在月经中期水平是高的,但黄体期PRL平均水平高于滤泡期,月经中期T和C值无明显变化,而滤泡期C水平(91ng/ml)高于黄体期(81ng/ml)。     

青光眼家兔及临床青光眼患者的房水与血浆心钠素水平研究

目的　为明确心钠素是否直接参与眼压的局部体液调节 ,并探讨房水中心钠素的来源。方法　对16例青光眼患者及 15只家兔青光眼模型用放射免疫方法进行房水和血浆心钠素含量的测定。结果　家兔 30眼房水心钠素平均含量 ,正常眼压时为 4 8 6 9± 2 1 5 3pg/ml,高眼压 2小时为 6 9 5 4± 32 98pg/ml,两者比较有显著差异(P <0 0 1) ;高眼压 4小时为 15 5 72± 72 34pg/ml,与正常眼压时比较差异显著 (P <0 0 1)。家兔血浆心钠素平均含量正常眼压时为 130 32± 4 7 5 4pg/ml,高眼压 2小时为 2 78 6 8± 5 4 4 4pg/ml,两者比较差异显著 (P <0 0 1) ;高眼压 4小时为 2 84 6 8± 6 3 78pg/ml,与正常眼压时比较差异显著 (P <0 0 1)。 16例青光眼患者眼压正常者房水心钠素含量为 72 31± 12 82pg/ml,而高眼压者房水心钠素含量为 180 4 4± 4 8 74pg/ml,两者比较差异显著 (P <0 0 1) ;眼压正常者血浆心钠素含量为 15 0 5 2± 4 5 0 7pg/ml,高眼压者血浆心钠素含量为 391 75± 92 37pg/ml,两者比较差异显著 (P<0 0 1)。结论　 1高眼压时血浆和房水的心钠素浓度均与眼压水平呈正相关 ,心钠素直接参与眼压的局部体液调节 ;2房水中的心钠素可能来源于血浆中的心钠素。     

原发性肾病综合征患者血清IL-2、IL-13的研究

目的　探讨原发性肾病综合征患者血清IL- 2、IL -13水平在肾病综合征发病中的作用及其临床意义。方法　采用ELISA法测定30例原发性肾病综合征患者口服强的松(1mg/kg) 8周治疗前后血清IL- 2、IL -13水平变化,并与正常对照组进行比较。同时对30例原发性肾病综合征患者进行肾活体组织检查。结果　30例肾病综合征患者治疗前血清IL 2水平(6 7.16±6 .5 5 )pg/ml,明显低于正常对照组(98.2 9±2 5 .96 )pg/ml(P <0 .0 5 ) ,血清IL 13水平(48.79±3.0 5 )pg/ml明显高于正常对照组(47.13±3.0 6 )pg/ml(P <0 .0 5 ) ;激素治疗后19例患者缓解(激素敏感型) ,11例患者无效(激素抵抗型)。激素敏感型患者治疗后血清IL- 2水平(72 .11±16 .95 )pg/ml与治疗前无显著差异,较正常对照组显著降低(P <0 .0 5 ) ,而治疗后血清IL 13水平(46 .37±3.32 )pg/ml较治疗前显著降低(P <0 .0 5 ) ,与正常对照组无显著差异;激素抵抗型患者治疗后血清IL- 2及IL-13水平均显著升高。结论　肾病综合征患者血清IL-2水平降低,血清IL- 13水平升高,两者均参与炎症过程,IL- 2、IL -13水平变化在一定程度上可提示病情变化,监测治疗效果。     

阻塞性睡眠呼吸暂停低通气综合征患者呼出气冷凝液中8-异前列腺素的检测及其临床意义

目的:探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者8-异前列腺素(8-isoPG)的变化及其临床意义?方法:收集40例OSAHS患者和30例健康对照者睡前和晨起的呼出气冷凝液(EBC)以及晨起的血清,应用ELISA法测定EBC和血清中的8-isoPG浓度,其中20例OSAHS患者经治疗3个月后,复查EBC和血清中的8-isoPG浓度?结果:① OSHAS组EBC中8-isoPG测定值睡前为(13.08 ± 1.42)pg/ml,晨起为(14.93 ± 1.39)pg/ml,差异有统计学意义(P < 0.01);对照组睡前为(11.06 + 0.72)pg/ml,晨起为(10.97 ± 0.70)pg/ml,差异无统计学意义(P > 0.05);2组晨起与睡前8-isoPG比较差异有统计学意义(P < 0.01);②血清中8-isoPG测定值OSHAS组晨起为(36.59 ± 14.89)pg/ml,对照组晨起为(19.91 ± 7.76)pg/ml,两组比较差异有统计学意义(P < 0.01);③OSAHS组患者晨起EBC与血清中8-isoPG水平成正相关(r=0.69,P < 0.01);④20例OSAHS患者经治疗(其中11例使用经鼻持续气道正压通气治疗,9例进行腭垂软腭咽成形术)3个月后,EBC中8-isoPG睡前为(12.13±1.39)pg/ml,晨起为(12.02 ± 1.36)pg/ml,晨起血清为(23.00 ± 9.54)pg/ml,分别与治疗前比较差异有统计学意义(P < 0.01);⑤OSAHS患者晨起EBC中8-isoPG浓度与睡眠呼吸暂停低通气指数(AHI)?颈围和腰围正相关(r值分别为0.65?0.43?0.34,P均< 0.05),与晨起血氧分压(PaO2)负相关(r=-0.31,P < 0.05)?结论:EBC中8-isoPG是反映OSAHS患者氧化应激反应的较好指标,对研究OSAHS发生机制?判断严重程度和评估治疗效果具有重要的临床意义?     

生殖激素检测在不孕症136例实验诊断中应用

目的:探讨生殖激素检测在不孕症妇女诊断中的应用价值。方法:采用全自动电化学发光分析系统对不孕症136例不同月经周期卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、催乳素(PRL)、孕酮(P)、睾酮(T)进行测定,并与正常育龄妇女比较。结果:与正常育龄妇女相比,不孕妇女卵泡期FSH(4.24±2.90mIU/ml)、E2(38.6±29.5pg/ml)降低,PRL(22.9±24.5ng/ml)升高;排卵期FSH(6.25±3.54mIu/ml)、LH(8.79±6.49pg/ml)、E2(87.3±65.7pg/ml)降低;黄体期P(9.46±2.36ng/ml)降低,PRL(98.9±41.0pg/ml)、LH(5.79±2.04mIu/ml)升高,差异均有统计学意义(P<0.05)。结论:6项生殖激素的检测对不孕妇女的诊断有重要作用。     

原发性肾病综合征患者治疗前后血及尿IL-8、IL-13水平测定及其临床意义

目的探讨肾病综合征患者治疗前后血清及尿中IL-8、IL-13水平变化及其临床意义.方法采用ELISA法测定25例肾病综合征患者血清及尿中IL-8、IL-13水平并与正常对照进行比较.口服强地松(1mg/kg)8～12w后再次测定血清及尿中IL-8、IL-13水平并与治疗前相比较.结果治疗前血清、尿IL-8水平[(31.08±3.07)pg/ml、(46.22±1.83)pg/ml]较正常对照[(22.99±2.29)pg/ml、(24.61±1.04)pg/ml]显著升高(P＜0.05),血清、尿IL-13水平[(31.05±2.71)pg/ml、(65.80±12.50)pg/ml]较正常对照[(25.61=2.52)pg/ml、(53.5±6.41)pg/ml]显著升高(P＜0.05).治疗后血清、尿IL-8水平[(23.06±2.60)pg/ml、(25.85±4.01)pg/ml]较治疗前显著降低(P＜0.05),血清、尿IL-13水平[(27.40±7.09)pg/ml、(50.31±11.13)pg/ml]较治疗前显著降低(P＜0.05),与正常对照无显著差异.结论肾病综合征患者,血清及尿中IL-8、IL-13水平升高,两者均参与炎症过程,并在疾病的发展中起一定的作用,激素治疗在控制病情的同时,可降低IL-8、IL-13水平,故IL-8、IL-13水平变化一定程度上可提示病情变化,监测治疗效果.     

抗人TNF-α单克隆抗体对银屑病患者血清TNF-α的中和研究

目的:研究抗人TNF-α单克隆抗体对寻常型银屑病患者血清TNF-α的中和效应。方法:应用双抗体夹心ELISA方法检测正常人及患者血清应用单克隆抗体中和前后TNF-α的浓度改变。应用L929细胞检测正常人和患者血清抗体中和前后的生物学活性变化情况。结果:正常人与银屑病患者血清TNF-α的浓度分别为:(3.146±6.184)pg/ml及(62.723±38.379)pg/ml;其生物学活性分别为:(6.817±5.086)%及(57.498±9.125)%。抗体中和后患者血清TNF-α的浓度为:(0.503±1.178)pg/ml;中和后的生物学活性为:(25.378±14.863)%。结论:寻常型银屑病患者血清中TNF-α的浓度及生物学活性均高于正常对照,而抗人TNF-α单克隆抗体在体外可以很好地中和患者血清中TNFα的浓度及其生物学活性。     

血清B型利钠肽水平对小儿呼吸困难的诊断价值

目的: 探讨血清B型利钠肽(B-type natriuretic peptide,BNP)浓度对判断小儿呼吸困难应用价值。方法: 用ELISA法检测有呼吸困难症状的心衰24例、肺炎23例、肺炎心衰18例、正常对照15名、肺炎心衰恢复期10例患儿的血清BNP浓度。结果: 心衰组BNP浓度(141.55±75.99) pg/ml高于肺炎心衰组(113.73±87.05) pg/ml、肺炎组(26.00±14.57) pg/ml及正常组(19.31±10.29) pg/ml (P<0.05~P<0.01);肺炎心衰组显著高于肺炎组及正常组(P<0.01),肺炎组与正常组比较差异无显著性(P>0.05)。结论: 检测血清B型利钠肽浓度对判断小儿心功能状态有一定参考价值。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.