HLA—Ia表达水平在预测干扰素治疗丙型肝炎疗效中的运用

目的：从丙型肝炎（HC）的免疫发病机制中寻找预测干扰素治疗HC疗效的有效指标。方法：在对32例慢性丙型肝炎（CHC）的患者运用干扰素治疗过程中,动态观察了血清中β2－微球蛋白（β2－MG）的变化;同时检测了30例正常人作为对照组。结果：CHC患者血清中β2－MG的含量明显高于正常组（P＜0．05）,干扰素治疗后β2－MG的表达量明显增加,其中完全应答者干扰素治疗后β2－MG的增加量明显高于无应答者或部分应答者（P＜0．05）。结论：CHC患者肝细胞表面的HLA－Ia类抗原的过度表达是造成肝损伤的原因之一,β2－MG可作为临床上预测干扰素疗效的简便,易行的指标之一。     

重症肌无力患者雷公藤多甙治疗后周围血细胞免疫功能变化的研究

目的 探讨重症肌无力（MG）患者周围血细胞免疫功能变化及雷公藤多甙（TⅡ）治疗MG的免疫调节机制,方法 将52例MG患者随机分为TⅡ治疗组及非TⅡ治疗组,观察两组前后及40例正常对照血清白细胞介素－6（IL－6）、肿瘤坏死因子（TNF）及可溶性白细胞介素2受体（sIL-2R)水平。结果 TⅡ治疗组及非TⅡ治疗组治疗前血汪IL－6、TNF及sIL-2R水平分别显著高于正常对照组,TⅡ治疗组治疗后血清IL－6、TNF及sIL-2R的水平均较治疗前明显降低,而非TⅡ治疗组治疗前后血清IL－6、TNF及sIL-2R水平无显著性差别。结论 MG患者存在明显的细胞免疫功能紊乱,TⅡ治疗MG的免疫机制可能是通过抑制IL－6、TNF及sIL-2R产生而发挥。     

原发性肾病综合征肾小管间质改变与激素疗效的相关分析

目的：探讨原发性肾病综合征（PNS）肾小管间质改变与激素疗效的关系；方法：采用放射免疫分析（RIA）法检测50例原发性肾病综合征患者激素治疗前后的尿β2微球蛋白（β2－MG）排泄量，同时用美国Techmicon RA-1000型全自动生化分析仪测定血清肌酐（Scr）等项目；结果：PNS患者尿β2－MG水平明显高于正常对照组（P＜0．01）；激素治疗后，激素敏感型患者尿β2－MG水平显示下降（P＜0．01），而激素依赖与激素无效型患者治疗前后无明显变化（P＞0．05）；结论：激素敏感型原发性肾病综合征患者中，合理的激素治疗可改善其伴有的肾小管间质病变。     

时间治疗学在老年高血压早期肾损害中的应用分析

目的：时间治疗学在老年高血压早期肾损害中的应用效果分析。方法：选择老年高血压合并微量蛋白尿患者93例，观察运用时间治疗学的方法，治疗前后的24小时动态血压，白昼及夜间的动态血压及其血压负荷，血、尿β2－微球蛋白（β2－MG）、尿微量白蛋白（mALB）。结果：能有效降低24小时平均收缩压、平均舒张压动态变化及白昼与夜间收缩压、舒张压，治疗前后均有显著差异（P〈0．01）。治疗后尿β2－MG、血β2－MG、尿-mALB均较治疗前有明显下降，差异有显著性（P〈0.01）。治疗前昼夜血压变化呈非杓型65例（69％），治疗后昼夜血压变化呈非杓型24例（25％），比较差异有统计学意义（P〈0．01）。治疗后昼夜血压变化节律，较治疗前有非常显著改变（P〈0．01）。结论：应用时间治疗学，血压能够控制到比较理想的水平，大多数患者可以恢复24小时血压的昼夜节律，降低靶器官损害。     

大剂量黄芪对慢性肾炎患者血、尿TNF—α的影响

目的：观察大剂量黄芪对慢性肾小球肾炎（CGN）患者血清、尿液肿瘤坏死因子－α（TNF－α）的影响。方法：采用放射免疫法测定30例CGN患者（治疗组）应用大剂量黄芪注射液治疗前后血清、尿液TNF－α水平;并与30例应用常规治疗的患者（对照组）及30名健康人（健康对照组）进行比较,同时观察尿β2－微球蛋白（β2－MG）、24小时尿蛋白定量及内生肌酐清除率（Ccr)的变化。结果：治疗前两组患者较健康对照组血、尿、TNF－α、尿β2－MG及24小时尿蛋白定量均显著升高（P＜0．01）,治疗后治疗组上述指标均明显改善（P＜0．01）,其改善优于对照组（P＜0．05,P＜0．01）。治疗后两组Ccr比较无明显差异（P＞0．05）。结论：大剂量黄芪有较好的降低CGN患者血、尿TNF－α作用,为该药治疗CGN提供了理论依据。     

肝炎后肝硬化患者血、尿β2—MG测定及临床意义

目的 探讨肝炎肝硬化患者血、尿β2微球蛋白（β2－MG）测定的临床意义。方法 采用放免法检测33例肝炎后肝硬化患者血、尿β2－MG浓度,并与30例正常人作比较。结果 肝硬化组血、尿β2－MG浓度明显高于对照组（P＜0.01）。结论 血、尿β2－MG检测可作为肝硬化诊断及观察病情变化的指标之一,并可用于预后估计。     

多发性骨髓瘤患者骨髓中程序化细胞死亡分子5 mRNA 的表达及意义

目的：观察多发性骨髓瘤（ MM）患者骨髓中程序化细胞死亡分子5（ PDCD5） mRNA的表达，并探讨其临床意义。方法检测20例MM患者骨髓中PDCD5 mRNA的表达量及血清β2－微球蛋白（β2－MG）和C反应蛋白（CRP）的水平，比较不同Durie－Salmon分期患者及5例患者治疗前后PDCD5 mRNA表达及β2－MG、CRP水平的差异。结果Ⅰ、Ⅱ、Ⅲ期患者骨髓PDCD5 mRNA表达量依次下降（P＜0．05，P＜0．01）；Ⅲ期患者血清β2－MG水平明显高于Ⅰ、Ⅱ期患者（P＜0．01），而Ⅰ、Ⅱ期患者之间比较差异无统计学意义（P＞0．05）；Ⅱ、Ⅲ期患者血清CRP水平明显高于Ⅰ期患者（P＜0．05）。 PDCD5 mRNA表达量与β2－MG水平（r＝－0．622，P＝0．003）、Durie－Salmon分期（r＝－0．791，P＜0．01）呈显著负相关，而与CRP水平未见显著相关性（P＞0．05）。5例患者经治疗在获得部分缓解后，其骨髓PDCD5 mRNA表达量较治疗前明显上升（P＜0．05），血清β2－MG、CRP水平虽较治疗前有下降趋势，但差异均无统计学意义（ P＞0．05）。结论 MM患者PDCD5 mRNA表达下调与临床分期密切相关，治疗后PDCD5 mRNA表达可上调。 PDCD5 mRNA与MM病情密切相关，可作为监测病情、判断疗效的一项重要指标。     

糖尿病患者血尿β2微球蛋白测定的临床价值探讨

用放射免疫法测定78例Ⅱ型糖尿病患者的血、尿β2微球蛋白（β2－MG），并研究其与糖尿病肾病的关系。结果表明：糖尿病患者的血、尿β2－MG均高于正常对照组．且其异常发生率高于BUN，Cr。血、尿β2－MG随病程延长阳性率升高。提示测定血、尿β2－MG可作为诊断早期糖尿病肾病的一项重要指标。     

尿α1-微球蛋白在诊断早期糖尿病肾病中的意义

目的：探讨尿α1－微球蛋白在诊断早期糖尿病肾病中的意义。方法：用125^I-α1－微球蛋白放射免疫法检测68例非胰岛领带型糖尿病（NIDDM）患者尿中尿α1－微球蛋白（α1－MG）和β2－微球蛋白（β2－MG）。结果：与正常对照组比较，NIDDM患者微量蛋白组尿α1－MG显著升高，结论：尿α1－MG可作为早期糖尿病肾病诊断的敏感指标。     

糖尿病患者检测β2－微球蛋白的临床意义

目的：探讨血、尿β2－微球蛋白（8rMG）对糖尿病患者的临床意义。方法：用放射免疫方法测定糖尿病患者血、尿β2－MG的水平。结果：糖尿病患者血、尿β2－MG含量明显高于健康人员（P〈0．01）,且随着病程的延长,血、尿β2－MG的含量明显增加;血、尿β2－MG的含量在非胰岛素依赖性糖尿病（NIDDM）和胰岛素依赖性糖尿病（IDDM）患者之间比较,差异无统计学意义（P〉0．05）。结论：测定糖尿病患者血、尿β2－MG对早期发现、预防和治疗糖尿病肾病有重要的临床意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.