苦参碱对小鼠免疫功能的影响

目的:观察苦参碱对小鼠脾淋巴细胞增殖和释放白细胞介素-2(IL-2)及腹腔巨噬细胞释放白细胞介素-1(IL-1)的影响.方法:溴化四唑蓝比色法测定脾淋巴细胞增殖,胸腺细胞增殖法和溴化四唑蓝比色法测定IL-1、IL-2活性.结果:苦参碱可明显抑制ConA和LPS诱导的小鼠脾淋巴细胞增殖及ConA诱导的小鼠脾细胞释放IL-2,对LPS诱导的小鼠腹腔巨噬细胞释放IL-1亦有一定的抑制作用.结论:苦参碱可抑制体外培养小鼠脾淋巴细胞增殖和释放IL-2及腹腔巨噬细胞释放IL-1.     

血小板激活因子对小鼠脾细胞增殖以及产生IL—2,NKCF和CSF的影响

本文研究了PAF对BALB/c小鼠脾细胞增殖以及产生IL-2,NKCF和CSF的影响。[~3H]TdR掺入法检测脾细胞增殖结果表明PAF在10~(-10)～10~(-7)mol/L范围内显著抑制ConA诱导的脾细胞增殖。用CTLL-2细胞测定IL-2,YAC-1细胞检测NKCF,小鼠骨髓细胞测定CSF,结果表明当PAF浓度为10~(-7)mol/L时,三种因子的产生均被明显抑制。PAF对小鼠脾细胞功能的抑制可能与其广泛的病理生理作用有关。     

沙棘总黄酮对淋巴细胞增殖和白细胞介素产生的影响

沙棘总黄酮(TFH)低浓度增强ConA、PHA、LPS诱导的小鼠脾淋巴细胞增殖和PHA诱导人外周血淋巴细胞增殖,高浓度则产生抑制;对IL-2的产生亦具有相似的作用,低浓度促进、高浓度抑制;对IL-1的产生则表现为明显增强作用。但在无丝裂原刺激的条件下,TFH对人和小鼠淋巴细胞增殖无明显影响。     

贞芪酪蛋白复合肽对免疫低下小鼠免疫调节的影响

目的观察贞芪酪蛋白复合肽对免疫低下小鼠免疫调节的影响.方法贞芪酪蛋白复合肽灌胃给药后,测定各组小鼠胸腺、脾指数,外周血白细胞数,脾T、B细胞增殖能力,脾细胞产生IL-2的能力以及血清和脾细胞溶血素含量等的变化.结果贞芪酪蛋白复合肽能显著提高免疫低下小鼠胸腺、脾指数及外周血白细胞数;能明显提高免疫低下小鼠脾T、B细胞增殖能力和脾细胞产生IL-2的能力;贞芪酪蛋白复合肽还能增加免疫低下小鼠血清和脾细胞溶血素的含量.结论贞芪酪蛋白复合肽是一种免疫调节剂,对免疫低下小鼠的免疫功能具有改善和提高的作用.     

雷公藤内酯醇对小鼠IL-产生和IL-2受体表达的影响

目的探讨雷公藤内酯醇对小鼠脾淋巴细胞IL-2产生和IL-2受体表达的影响.方法应用体外细胞培养和APAAP桥联酶标法检测了雷公藤内酯醇对刀豆蛋白A(ConA)诱导的小鼠脾淋巴细胞白细胞介素(IL-2)产生及IL-2受体表达的影响.结果雷公藤内酯醇对IL-2产生无影响,但对IL-2受体表达有明显的抑制作用.结论雷公藤内酯醇抗免疫排斥作用主要是通过抑制IL-2受体表达,从而使IL-2与IL-2受体的相互作用受到影响,最终抑制了淋巴细胞增殖.     

体内灌注灵芝对小鼠免疫功能的影响

本文通过小鼠胃内灌注灵芝提取液，检测小鼠脾细胞白细胞-2(IL-2)分泌活性和淋巴细胞增殖反应，表明灵芝能提高脾细胞分泌自细胞介素-2(IL-2）并对淋巴细胞增殖具有促进作用，提示灵芝提取液经胃内给药速经能增强小鼠细胞免疫功能。     

藏药结血蒿水提物的免疫抑制作用研究

目的研究结血蒿水提取物（AV-ext）对小鼠免疫反应的抑制作用。方法运用淋巴细胞增殖反应、混合淋巴细胞反应观察AV-ext体外应用和口服给药对小鼠脾细胞增殖的影响；运用小鼠脾细胞增殖试验，观察AV-ext体外应用对小鼠活化脾细胞分泌IL-2的影响；用明胶酶谱法和粘附分析法，观察AV-ext对小鼠活化T淋巴细胞移动和粘附能力的影响。结果AV-ext在1μg／mL-100μg／mL剂量范围内对脾细胞无明显的细胞毒性，但对刀豆蛋白A（ConA）诱导的小鼠脾细胞增殖反应、混合淋巴细胞反应具有明显的抑制作用，对小鼠活化脾细胞分泌IL-2、小鼠活化T淋巴细胞分泌基质金属蛋白酶9（MMP-9）及粘附能力有显著的抑制作用；连续7d每日口服给予150mg／kg和300mg／kg的AV-ext，能明显抑制小鼠脾细胞增殖及活化脾细胞分泌MMP-9。结论AV-ext能显著抑制小鼠的细胞免疫反应。     

狼毒大戟多糖对小鼠脾淋巴细胞增殖及细胞因子分泌的影响

目的:探讨狼毒大戟多糖(EFP-AW1)对小鼠脾淋巴细胞增殖及细胞因子白介素2(IL-2)和干扰素γ(IFN-γ)分泌的影响。方法:制备小鼠脾淋巴细胞,加入EFP-AW1,使终质量浓度分别为0、25、50、100、200 mg/L,再加入Con A,培养60 h后,采用MTT法检测不同质量浓度狼毒大戟多糖EFP-AW1对Con A诱导的小鼠脾淋巴细胞增殖反应的影响;将5种不同浓度的EFP-AW1分别加入小鼠脾淋巴细胞培养体系,再加入Con A,培养48 h后,收集培养上清,ELISA法检测不同质量浓度EFP-AW1对小鼠脾淋巴细胞分泌IL-2和IFN-γ的影响。结果:与空白组比较,50、100、200 mg/L的狼毒大戟多糖能够促进Con A诱导的小鼠脾淋巴细胞增殖反应(P0.05),并能够提高脾淋巴细胞产生IL-2和IFN-γ的水平(P0.05)。结论:狼毒大戟多糖具有一定的免疫增强作用。     

软枣猕猴桃茎多糖对小鼠脾细胞功能的影响

<正> 我们以软枣猕猴桃(AaP)茎为原料,提取水溶性多糖,观察了它对小鼠淋巴细胞增殖反应以及白细胞介素2(IL-2)产生的影响,初步结果如下。 1 体外试验 ①以~3H-TdR掺入法观察AaP多糖对小鼠脾细胞增殖反应的影响。结果表明,Aap多糖单独即有轻度刺激脾细胞增殖的作用,并能明显增强小鼠脾细胞对ConA(5μg/ml)刺激的反应性。这种     

鸡Ⅱ型胶原喷雾剂对大鼠佐剂性关节炎的免疫调节作用

目的观察鸡Ⅱ型胶原(CⅡ)喷雾剂对大鼠佐剂性关节炎(AA)的免疫调节作用.方法用CFA诱导大鼠AA模型; MTT法检测脾淋巴细胞增殖反应; IL-1、IL-2活性的检测采用小鼠胸腺细胞增殖法.结果 AA大鼠脾淋巴细胞增殖反应、IL-2活性均下降,腹腔巨噬细胞(PMФ)分泌IL-1升高;CⅡ 5、10、20 μg/kg治疗给药进一步降低脾淋巴细胞增殖反应及IL-2的活性,对PMФ产生高水平的IL-1有抑制作用;体外CⅡ 0.1、0.2、0.4、0.8 mg/L对LPS诱导的滑膜细胞产生IL-1水平无影响.结论鸡CⅡ对大鼠AA异常免疫功能具有调节作用,其机制可能与其诱导免疫耐受有关.     

脂质血管生长因子粗制品对小鼠脾细胞增殖及ConA诱导的IL-2水平的影响

建立了脂质血管生长因子（LAF）的乳化方法，并研究了乳化的脂质血管生长因子对小鼠腺细胞及ConA诱导的IL-2水平的影响。结果显示：MF能明显抑制小鼠脾细胞的增殖且降低IL-2的产生水平，而对照的皮下脂肪抽提物则无此效应。同时发现，乳化过程中加入的Tween-80有一定的细胞毒性。     

Strong additive effect of calcitriol and cyclosporine A on lymphocyte proliferation in vitro and rat liver allotransplantations in vivo

Background Vitamin D3 and its metabolites have been found to exert immunosuppressive effects both in vivo and in vitro. We investigated the synergistic effect of calcitriol and cyclosporine A (CsA) on lymphocyte proliferation in vitro and graft rejection following rat liver allotransplantations in vivo.Methods Alloantigen driven, human peripheral mononuclear cells’ proliferation and cytokine production capacity were tested in the presence or absence of various concentrations of calcitriol or CsA. In vivo, liver allografts were transplanted in a high responder strain combination (SD to Wistar) rats and combination of subtherapeutical dose of CsA and calcitriol was administered in recipients, whereas the control recipients received single or no immunosuppressant. Proliferation of splenocyte from recipient was tested with mixed lymphocyte reaction. Serum interleukin-2 (IL-2) and interferon gamma (IFN-γ) concentrations were measured with enzyme linked immunosorbent assay. Results Combined medication of 10(-9) mol/L calcitriol and 100 ng/ml CsA inhibited human peripheral mononuclear cells’ proliferation to alloantigen and the production of IL-2 and IFN-γ but promoted that of IL-4 and IL-10. Similarly, combination of 250 ng·kg(-1)·d(-1) calcitriol and 1.0 mg·kg(-1)·d(-1) CsA showed an additive effect in liver transplant model. It restrained splenocyte proliferation to alloantigen from donor and significantly reduced serum concentration of IL-2 and IFN-γ in recipients. Consequently, allograft rejection in combined medication group was minor (median William’s grade was 1.0 vs 3.0 in combined medication group and in the control group, P&lt;0.05) and the recipients’ survival was evidently prolonged [(93.7±5.8) days vs (12.6±1.4) days in combined medication group and in the control group, P&lt;0.01].Conclusion A combination of calcitriol and CsA has an additive effect on limiting lymphocyte proliferation and prolonging liver graft survival. With its additional immunomodulating property, calcitriol is a potent immunosuppressant that can extend the therapeutic window of classical immunomodulators in prevention and treatment of liver graft rejection.     

复方别敏中黄芩苷、淫羊藿苷和黄芪甲苷配伍比例的优化

目的：探讨复方“别敏”中黄芩苷、淫羊藿苷与黄芪甲苷3种成分合用的优化配伍比例。 方法：采用卵清蛋白腹腔注射建立变应性鼻炎小鼠模型,以脾淋巴细胞增殖反应为指标,利用四唑氮氢氧化物法确定黄芩苷、淫羊藿苷、黄芪甲苷各成分的有效剂量范围;选用U＊10（108）表组方设计所得的3种成分的10种组合进行干预,考察其对脾淋巴细胞增殖的抑制效应,经逐步回归统计分析,获得3种成分合用的最佳配比;通过淋巴细胞增殖反应和对淋巴细胞培养上清液中细胞因子含量检测,验证3种成分的最佳配伍效应是否优于各单一成分。 结果：2~10 μmol/L黄芩苷、2~10 μmol/L淫羊藿苷和1~10 μmol/L黄芪甲苷明显抑制致敏小鼠脾淋巴细胞增殖;当黄芩苷、淫羊藿苷、黄芪甲苷以1∶2.14∶2.65比例配伍时,抑制细胞增殖的效应最明显;进一步的验证研究证实,该配伍对ConA刺激的淋巴细胞增殖的抑制效果及对淋巴细胞上清液中白细胞介素4（interleukin-4,IL-4）、IL-5和γ干扰素（interferon-gamma, IFN-γ）的调节作用明显优于黄芩苷、淫羊藿苷、黄芪甲苷单用的效果（P〈0.05,P〈0.01）。 结论：复方“别敏”中主要有效成分黄芩苷、淫羊藿苷、黄芪甲苷按优化比例合用可最有效地抑制致敏小鼠脾淋巴细胞增殖,调节因变态反应引起的Th1、Th2型细胞因子失衡。     

Impact of psychosocial stress on airway inflammation and its mechanism in a murine model of allergic asthma

Background  It has already been recognized that psychosocial stress evokes asthma exacerbation; however, the mechanism of how stress gets inside the body is not clear. This study aimed to observe the impact of psychosocial stress on airway inflammation and its mechanism in the ovalbumin-induced asthmatic mice combined with social disruption stress.

Methods  Thirty-six male BALB/c mice were randomly divided into: control group, asthma group (ovalbumin-induced), asthma plus social disruption stress group (SDR), and SDR group. The open field video tracking system was used to assess animal behaviors. The invasive pulmonary resistance (RL) and dynamic lung compliance (cdyn) test system from Buxco was applied to detect pulmonary function. The enzyme-linked immunosorbent assay (ELISA) was utilized to determine OVA-IgE, T-helper type 2 (Th2) cytokines (IL-4, IL-5, IL-13) and corticosterone in mouse serum, the Th2 cytokines (IL-4, IL-5, IL-13, IL-6, TNF-α) in bronchoalveolar lavage fluid (BALF), and IL-6 and TNF-α levels in the supernatant of splenocytes cultured in vitro. Hematoxylin-eosin (H&E) staining was used to assess airway inflammation in lung histology. The cell count kit-8 assay (CCK-8) was applied to evaluate the inhibitory effect of corticosterone on splenocyte proliferation induced by lipopolysaccharide (LPS). Real time-PCR and Western blotting were utilized to determine glucocorticoid receptor (GR) mRNA and GR protein expression in lungs.

Results  The open field test showed that combined allergen exposure and repeated stress significantly shortened the time the mice spent in the center of the open field (P <0.01), increased ambulatory activity (P <0.01) and the count of fecal boli (P <0.01), but deceased vertical activity (P <0.01). Results from pulmonary function demonstrated that airway hyperresponsiveness (AHR) was enhanced by psychosocial stress compared with allergy exposure alone. The ELISA results showed that cytokines in serum and BALF were significantly increased (P <0.05). Moreover, the lung histology showed that infiltrated inflammatory cells were significantly increased in the asthma-SDR group compared with the asthma group (P <0.05). Interestingly, serum corticosterone was remarkably raised by psychosocial stress (P <0.05). In addition, the inhibitory effect of corticosterone on IL-6 and TNF-α in LPS-stimulated splenocyte cultures in vitro was diminished in the asthma-SDR group compared to the asthma group. The CCK-8 test revealed that the inhibition effect of corticosterone on splenocyte proliferation induced by LPS was significantly impaired in the SDR and asthma-SDR groups, while no significant effect was observed in the control and asthma groups. Furthermore, expression of GR mRNA and GR protein were significantly reduced in the lung tissues of the asthma-SDR group (P <0.05).

Conclusions  Social disruption stress can promote anxiety behavior, activate the hypothalamic-pituitary-adrenal (HPA) axis, increase AHR and inflammation, and also impair glucocorticoid sensitivity and its function in a murine model of asthma. The down-regulation of GR expression induced by social disruption stress is in part associated with glucocorticoid insensitivity, which leads to asthma exacerbation.

     

肺岩宁方对人肺癌细胞系增殖及肿瘤坏死因子α诱导的白细胞介素6和白细胞介素8产物的影响

目的：探讨中药肺岩宁方含药血清对人肺癌细胞系A549细胞增殖及肿瘤坏死因子α（tumor necrosis factor—α,TNF—α）诱导的A549细胞分泌自细胞介素6（interleukin-6,IL-6）和IL-8的影响。 方法：肺岩宁方含药血清培养A549细胞24、48和72h后,采用四唑单钠盐法检测肺岩宁方含药血清对A549细胞增殖的影响;肺岩宁方含药血清培养A549细胞48h后,酶联免疫吸附测定法检测肺岩宁方含药血清对TNF—α诱导的IL-6和IL-8分泌的影响,逆转录聚合酶链式反应检测IL-6和IL-8 mRNA表达水平的变化。 结果：肺岩宁方含药血清对A549细胞增殖具有明显的抑制作用。1μg／LTNF—α可明显提高A549细胞上清液中IL-6和IL-8的分泌水平,肺岩宁方含药血清与TNF-α共同作用后,可抑制IL-6和IL-8的分泌（P〈0．01）及IL-6、IL-8mRNA的表达（P〈0．01）。 结论：肺岩宁方含药血清对TNFα诱导A549细胞分泌IL-6和IL-8具有明显的抑制作用,这为其临床治疗肺癌提供了一定的实验依据。     

小鼠免疫细胞活性与光滑假丝酵母菌胞外多糖关系的研究

目的:探讨光滑假丝酵母菌胞外多糖对小鼠免疫细胞活性的影响。方法:采用四甲基偶氮唑盐(MTT)法和酶联免疫吸附试验(ELISA)分别检测该胞外多糖对小鼠脾细胞增殖的刺激作用和对小鼠脾细胞分泌白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)的刺激作用,并观察该胞外多糖对小鼠NK细胞杀伤活性的影响。结果:产自海南红树林的光滑假丝酵母菌胞外多糖在体外能显著刺激小鼠脾细胞增殖;显著增强小鼠脾细胞分泌IL-2和IFN-γ的能力;另外该胞外多糖还具有促进NK细胞杀伤K562细胞的能力。结论:产自海南红树林的光滑假丝酵母菌胞外多糖具有增强小鼠免疫功能的作用。     

Effects and mechanisms of Cryptotanshinone on rats with adjuvant arthritis

Background  Cryptotanshinone (CT) is the major active constituent of Salvia miltiorrhiza Bunge. The present study was carried out to investigate the effects of CT on rats with adjuvant arthritis (AA).

Methods  AA was induced by the metatarsal footpad injection with complete Freund’s adjuvant in male Sprague-Dawley rats. The secondary inflammatory reaction was evaluated by hind paw swelling and the polyarthritis index. Activity of interleukin-1 (IL-1) was detected by the concanavalin A-induced thymocytes proliferation assay. The lymphocytes proliferation and IL-2 production were assayed by 3-(4,5-2dimethylthiazal-2yl)2,5-diphenyltetrazoliumbromide (MTT) and activated mouse splenocytes proliferation, respectively.

Results  Intragastric administration of CT (50 and 100 mg/kg) significantly decreased secondary inflammatory reactions and increased the spleen and thymus index. There was a marked immunologic and inflammatory response in the AA model, which was accompanied by the decrease of thymocyte proliferation and IL-2 production as well as the increase of IL-1 production. CT apparently enhanced thymocyte proliferation and decreased IL-1 production in AA rats.

Conclusion  These results indicate that CT may exert its anti-inflammatory and immunoregulatory effects through inhibiting lymphocyte proliferation and production of pro-inflammatory mediators.

     

Experimental Studyon Anti－tumor Effect of SplenocytesInduced by Anti－CD3McAb，PHA and IL－2

（沈张悦）（邵静芳）（王晓林）（朱慧芬）ＥｘｐｅｒｉｍｅｎｔａｌＳｔｕｄｙｏｎＡｎｔｉ－ｔｕｍｏｒＥｆｆｅｃｔｏｆＳｐｌｅｎｏｃｙｔｅｓＩｎｄｕｃｅｄｂｙＡｎｔｉ－ＣＤ３ＭｃＡｂ，ＰＨＡａｎｄＩＬ－２￥ＳＨＥＮＧｕａｎ－ｘｉｎ，ＷＡＮＧＸｉａｏ－ｌｉ...     

重组人白细胞介素10单克隆抗体对角朊细胞增殖及产生细胞因子的影响

目的 研究重组人白细胞介素10单克隆抗体对无血清培养的角朊细胞增殖、产生细胞因子的影响。方法 用四甲基偶氮唑蓝比色法(MTT)测定对细胞增殖的影响;用小鼠胸腺细胞增殖法检测IL-1;ELISA法检测IL-6、IL-8。结果 抗重组人白细胞介素10单克隆抗体促进角朊细胞增殖与IL-1、IL-6及IL-8的分泌,并呈剂量依赖关系。结论 重组人白细胞介素10单克隆抗体促进角朊细胞增殖与细胞因子分泌。