钙离子对维生素K3镇痛作用的影响

本文研究Ｃａ２＋对ＶｉｔＫ３镇痛作用的影响．在小白鼠辐射热甩尾模型上Ｖｉｔｋ３（６４ｍｇ／ｋｇ，ｉｐ）’：有明显的镇痛作用（Ｐ＜０．０１），这种作用能被ＣａＣＩ２（１．０Ｘ１０－７ｍｏｌ，ｉｃｖ）拮抗（Ｐ＜０．０５），被Ｃａ２＋络合剂ＥＧＴＡ（５．２６×１０－７ｍｏｌ　／ｋｇ，　ｉｃｖ）和Ｃａ２＋通道阻滞剂维拉帕米２５ｍｇ／ｋｇ，ｉｃｖ）增强（Ｐ值接近０．０５）。结果提示，细胞内外Ｃａ２＋浓度影响ＶｉｔＫ３的镇痛作用．     

C—AEO—I膜性材料毒性动物实验结果报告

目的：观察Ｃ－ＡＥＯ－Ｉ对人体的毒性作用，评价其安全性。方法：采用急性毒性试验、口服蓄积毒性试验，皮肤刺激试验、皮肤过敏试验、小鼠骨髓细胞微进行观察。结果：（１）ＬＤ５０为１３０６ｍｇ／ｋｇ，雄性１２２０ｍｇ／ｋｇ，雌性１３４６ｍｇ／ｋｇ；（２）蓄积系数粝于５．３，无皮肤刺激反应及皮肤过敏反应发生；（３）不会诱发微核 核的变化。结论：Ｃ－ＡＥＯ－Ｉ膜性材料是安全性的。     

一氧化氮合酶抑制剂和一氧化氮供体对沙土鼠缺血性脑损伤的影响

目的：研究一氧化氮（ＮＯ）在缺血性脑损伤中的作用。方法：夹闭沙土鼠双侧颈总动脉２０ ｍ ｉｎ 制备前脑缺血性脑损伤模型,分生化组和病理组。每组又分：（１） 假手术组;（２） 缺血对照组; （３） 硝基－Ｌ－精氨酸（ＬＮＮＡ）组,分３种剂量（３,２０,５０ ｍ ｇ／ｋｇ ｉｐ）;（４） Ｌ－精氨酸（Ｌ－Ａｒｇ）组,３００ ｍ ｇ／ｋｇ ｉｐ。第１,２组仅ｉｐ 等量生理盐水。结果：缺血性损伤后脑含水量增加,一氧化氮合酶（ＮＯＳ）活性明显升高,ＬＮＮＡ剂量依赖性抑制ＮＯＳ活性,小剂量能明显减轻脑水肿。小、中剂量ＬＮＮＡ 能减轻缺血性损伤后海马ＣＡ１区和ＣＡ２区锥体细胞缺失,小剂量作用明显,大剂量加重细胞缺失。结论：脑缺血后ＮＯＳ活性明显增加,加重缺血性脑损伤。通过ＬＮＮＡ适当降低脑组织ＮＯＳ活性能明显减轻脑水肿和海马区细胞坏死,对脑损伤有保护作用     

唐古特大黄多糖对小鼠急性肝损伤的保护作用

目的：观察唐古特大黄多糖（ＴＭＰ）对小鼠急性肝损伤的保护作用及探讨ＴＭＰ对肝损伤的保护机理。方法：小鼠６０只,随机分６组,生理盐水正常对照组,药物模型组,生理盐水模型对照组和３个给药组,每组按要求分别用ＣＣｌ４ １５６ｍｇ／ｋｇ,Ｄ－Ｇａｌ－Ｎ８００ｍｇ／ｋｇ,ＴＡＡ５００ｍｇ／ｋｇ腹腔注射（ｉｐ）,造成小鼠急性肝损伤,以ＴＭＰ１００ｍｇ／ｋｇ,２００ｍｇ／ｋｇ,４００ｍｇ／ｋｇ×５ｄ灌胃,用硫代     

氯胺酮与吗啡配伍用于硬膜外术后镇痛时剂量的探讨

将５０ 例患者随机分为５ 组进行观察。结果：Ａ 组吗啡２ ｍ ｇ ;Ｂ 组：吗啡２ ｍｇ 加氯胺酮０ ．８ ｍ ｇ／ｋｇ ;Ｃ 组：吗啡２ ｍ ｇ加氯胺酮１ ．０ ｍ ｇ／ｋｇ ;Ｄ 组：吗啡２ ｍ ｇ 加氯胺酮１ ．２ ｍｇ／ｋｇ ;Ｅ 组：吗啡２ ｍｇ 加氯胺酮１ ．５ ｍ ｇ／ｋｇ 。术后随访有效镇痛时间及副作用。Ｂ 组有效镇痛时间比Ａ 组未见明显延长,Ｃ、Ｄ 组有效镇痛时间明显长于Ａ 组,副作用未见增加,Ｅ 组有效镇痛时间虽延长,副作用发生率明显增加。认为氯胺酮与吗啡配伍用于硬膜外腔术后镇痛能延长镇痛时间,氨胺酮１ ．２ ｍ ｇ／ｋｇ 加吗啡２ ｍｇ 是较为适宜的剂量     

异丙酚全凭静脉麻醉用于腹腔镜胆囊切除术的临床观察

为观察异丙酚全凭静脉麻醉用于腹腔镜胆囊切除术的麻醉经过及苏醒情况,重点观察麻醉诱导插管及ＣＯ２ 人工气腹过程呼吸循环功能的变化,以探讨其优点和实用性。对３０ 例ＡＳＡ Ⅰ～Ⅱ级择期腹腔镜胆囊切除术病人,以芬太尼２ ～４μｇ·ｋｇ －１ 及异丙酚２ ～２ ．５ ｍｇ·ｋｇ － １、卡肌宁０ ．５ ｍｇ／ｋｇ － １静注快速诱导气管插管,用微量泵持续静脉泵注异丙酚１０ ～１２ ｍ ｇ·ｋｇ －１·ｈ －１ 、２０ ～３０ ｍｉｎ 间断静注卡肌宁０ ．２５ ～０ ．５ ｍｇ／ｋｇ － １ 维持麻醉及肌松,连续监测Ｆｉｏ２、ＳｐＯ２ 、ＰＥＴＣＯ２ 、ＴＶ、Ｐｐｅａｋ 、ｍ Ｐａｗ 、ＨＲ、ＳＢＰ、ＤＢＰ、ＭＡＰ、ＥＣＧ 的变化。结果本组平均麻醉时间为７９ ．５ ±５ ．２ ｍｉｎ ,异丙酚平均总用量为６０５ ±１３５ ｍｇ ,诱导后插管前ＳＢＰ、ＭＡＰ、ＨＲ 分别下降３ ．６３ｋＰａ 、１ ．９８ｋＰａ 、１７ 次／ ｍｉｎ（ Ｐ ＜ ０ ．０１） ,ＤＢＰ 也下降１ ．２５ｋＰａ（ Ｐ ＜ ０ ．０５） ,完成插管后迅速回升;ＰＥＴＣＯ２ 、Ｐｐｅａｋ 、ｍ Ｐａｗ 在ＣＯ２ 气腹后１０ ～１５ ｍｉｎ 上升到最高值（ Ｐ ＜ ０ ．０１） ,并在气腹期维持在较高水平。全部病例均于拔管后     

青蒿素衍生物8—2A的抗疟作用及毒性

青蒿素衍生物８—２Ａ为对乙酰氨基苯磺酸酯，系我所研制的有效抗疟青蒿素衍生物。对ＰｌａｓｍｏｄｉｕｍｂｅｒｇｈｅｉＮｓｔｒａｉｎ的ＥＤ＿（５０），ＰＯ：６４．２７，ｉｐ：３８．１１，ｉｍ：２６．３６（ｍｇ／ｋｇ），急性毒性试验，ＬＤ＿（５０），ｐｏ：３１４３．９０，ｉｐ：１５０７．０５，ｉｍ：２２８０．３７（ｍｇ／ｋｇ）。与Ａ—Ａ比较，对鼠疟有更好的疗效及较高的治疗指数和安全系数。长期毒性试验，分别以５４，１０８，２１６（ｍｇ／ｋｇ／ｄ×１４ｄ，ｉｍ），大白鼠的体重较对照组为低，外周血内出现有核红细胞０～１／１视野及心肌损伤或供血不足。病理形态学检查：中剂量组出现肝、肾轻微炎症反应，大剂量组出现肝、肾可逆性变性。豚鼠过敏反应阴性。小鼠致畸试验：每８４０ｍｇ／ｋｇ共１０日，出现死胎１只。     

三七总皂甙对动物脑缺血性损伤的保护作用

观察三七总皂甙（ＳａｐｏｎｉｎｓｏｆＰａｎａｘｎｏｔｏｇｉｎｓｅｎｇＰＮＳ）对小鼠全脑缺血和大鼠局灶性脑缺血（ＭＣＡＯ）的影响。结果发现ＰＮＳ（５０、１００ｍｇ．ｋｇ－１×３ｄ，ｉｐ）明显延长断头或ｉｖ饱和ＭｇＣｌ２后喘息持续时间。ＰＮＳ２００ｍｇ．ｋｇ－１术前３０ｍｉｎ或ＭＣＡＯ术后１５ｍｉｎｉｐ能减少ＭＣＡＯ术后２４ｈ脑梗塞面积，改善神经功能障碍及行为异常，减轻神经细胞缺血性损害。提示ＰＮＳ对缺血性脑损伤有保护作用。     

黄芪多糖对小鼠实验性肝损伤的保护作用

黄芪多糖（ＡＳＧ）５０、１００ｍｇ·ｋｇ－１×６ｄｉｍ可明显对抗四氯化碳（ＣＣｌ４，０．０１５％花生油溶液，１０ｍｌ·ｋｇ－１，ｉｐ），扑热息痛（ＡＡＰ，５５０ｍｇ·ｋｇ－１，ｉｐ）和无水乙醇（０．５ｇ．ｋｇ－１，ｉｇ）引起的小鼠血清谷丙转氨酸和谷草转氨酶的升高，对ＣＣｌ４，及ＡＡＰ引起的小鼠病理组织改变有明显保护作用。     

Χ－硒化卡拉胶对小鼠空间分辨学习的影响

用Ｙ型迷宫箱测试小鼠空间分辨能力，结果：ｐｏ　－硒化卡拉胶（　－ｓｅｌｅｎｏｃａｒｒａｇｅｅ－ｎａｎ，　－ＳｅＣ）１８０ｍｇ／ｋｇ及１０８ｍｇ／ｋｇ（ｑｄ×１４ｄ）能增强正常小鼠的学习能力，提高学习成绩；ｐｏ　－ＳｅＣ１８０、１０８及６４ｍｇ／ｋｇ（ｑｄ×１４）对东莨菪碱（ｉｐ４ｍｇ／ｋｇ）所致小鼠学习障碍均有拮抗作用；ｐｏ　－ＳｅＣ１８０ｍｇ／ｋｇ（ｑｄ×１４）对东莨菪碱（ｉｐ５ｍｇ／ｋｇ）所致小鼠记忆保持障碍有改善作用。以上表明　－ＳｅＣ对脑的学习功能有促进作用，并能拮抗药物引起的学习与记忆功能的损害，改善大脑功能．     

硫乙内酰脲衍生物的合成与镇痛作用

已知某些硫乙内酰脲的镇痛作用与脑啡肽酶抑制有关系, 为寻找无成瘾性的强效镇痛药,以氨基酸和硫代异氰酸苄酯为原料,合成了６ 个（ ±）５氨基酸残基３苄基２硫代乙内酰脲（ 简称 Ｂ Ｔ Ｈ Ａ Ａ） 化合物。经小鼠热板试验发现,所合成的化合物均有一定的镇痛活性。     

硫乙内酰脲衍生物的合成与镇痛作用

为了寻找强效镇痛药物,根据脑啡肽酶抑制机理,以DL-氨基酸和邻取代硫代异氰酸苯酯为原料,合成了19个硫乙内酰脲衍生物:(±)5-氨基酸残基-3-邻取代苯基-2-硫代-乙内酰脲(简称o-取代-PTH-AA)。经小鼠热板试验发现,o-OCH_3-PTH-His-HCl(YX-7)(100mg/kg,ip)的镇痛作用强度与盐酸吗啡(20mg/kg,ip)相当。     

硫乙内酰脲衍生物的合成与镇痛作用

在已知某些硫乙内酰脲(PTH-AA)的镇痛作用与脑啡肽酶抑制关系的认识基础上,以DL-氨基酸和含氟取代硫代异氰酸苯酯为原料,合成了17个硫乙内酰脲衍生物:(±)5-氨基酸残基-3-取代苯基-2-硫代-乙内酰脲(简称取代-PTH-AA)。经小白鼠热板试验发现,3-CF_3-PTH-Ala(YZ_1)(100 mg/kg,ip)、3-Cl-4-F-PTH-Ala(YZ_8)(100 mg/kg,ip)和3-Cl-4-F-PTH-Arg-HCl(YZ_(15)(100 mg/kg,ip)具有较强的镇痛作用,与盐酸吗啡(40 mg/kg,ip)相当。     

硫脲基乙酸衍生物的合成与镇痛作用

脑啡肽酶抑制剂(Enkephalinase Inhibitors)较目前临床使用的麻醉性镇痛药物具有镇痛作用强、副作用少、无成瘾性等优点,可能成为下一代临床应用的镇痛药物。据报道,     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.