Response of Gli1+ Suture Stem Cells to Mechanical Force Upon Suture Expansion

Normal development of craniofacial sutures is crucial for cranial and facial growth in all three dimensions. These sutures provide a unique niche for suture stem cells (SuSCs), which are indispensable for homeostasis, damage repair, as well as stress balance. Expansion appliances are now routinely used to treat underdevelopment of the skull and maxilla, stimulating the craniofacial sutures through distraction osteogenesis. However, various treatment challenges exist due to a lack of full understanding of the mechanism through which mechanical forces stimulate suture and bone remodeling. To address this issue, we first identified crucial steps in the cycle of suture and bone remodeling based on the established standard suture expansion model. Observed spatiotemporal morphological changes revealed that the remodeling cycle is approximately 3 to 4 weeks, with collagen restoration proceeding more rapidly. Next, we traced the fate of the Gli1⁺ SuSCs lineage upon application of tensile force in three dimensions. SuSCs were rapidly activated and greatly contributed to bone remodeling within 1 month. Furthermore, we confirmed the presence of Wnt activity within Gli1⁺ SuSCs based on the high co-expression ratio of Gli1⁺ cells and Axin2⁺ cells, which also indicated the homogeneity and heterogeneity of two cell groups. Because Wnt signaling in the sutures is highly upregulated upon tensile force loading, conditional knockout of β-catenin largely restricted the activation of Gli1⁺ SuSCs and suppressed bone remodeling under physiological and expansion conditions. Thus, we concluded that Gli1⁺ SuSCs play essential roles in suture and bone remodeling stimulated by mechanical force and that Wnt signaling is crucial to this process. © 2022 American Society for Bone and Mineral Research (ASBMR).     

螺旋上升式教学模式在儿科住院医师规范化培训中的应用效果

目的 探讨螺旋上升式教学模式在儿科住院医师规范化培训中的应用效果.方法 选取2017年9月至2020年6月我院儿科接受规范化培训的住院医师90名,采用随机数字表法将其分为对照组和观察组,各45名.对照组采用传统教学模式,观察组采用螺旋上升式教学模式.比较两组的临床理论、临床技能评分、胜任力及教学满意度.结果 观察组的临床理论、临床技能评分高于对照组(P<0.05).观察组的批判性思维能力、精于专业能力、有效沟通能力、医德高尚能力、专业发展能力评分高于对照组(P<0.05).观察组对激发学习兴致、增强自主学习规划、提升科研设计水平、提高独立思考水平的满意度高于对照组(P<0.05).结论 螺旋上升式教学模式在儿科住院医师规范化培训中的应用效果较好,可提升临床理论、临床技能掌握程度,提高胜任力及教学满意度.     

Selected Abstracts from the 13th Annual Meeting of the Clinical Immunology Society: 2022 Annual Meeting: Immune Deficiency and Dysregulation North American Conference

Journal of Clinical Immunology - Coronavirus disease 2019 (COVID-19) exhibits a wide spectrum of clinical manifestations, ranging from asymptomatic to critical conditions. Understanding the...     

体针及其配合推拿对单纯性肥胖女性体质量及BMI改变的影响

目的:评价体针配合推拿以及单用体针对单纯性肥胖女性体质量及身体质量指数(Body Mass Index,BMI)的影响。方法:将56例BMI≥25的单纯性肥胖女性随机分为两组,一组予以体针配合推拿治疗,另一组予以单纯体针治疗,每日治疗1次,持续21天。每日记录体质量和BMI的变化。结果:参与治疗的单纯性肥胖女性,体质量和BMI在前4天降低最明显,与治疗前对比,体针配合推拿与单独体针治疗均能显著地降低患者的体质量和BMI(P0.05),但两种治疗方法并无显著性差异。结论:从经济策略来看,单用体针治疗单纯性肥胖是更为合理的选择。     

基于网络药理学的黄精丸干预糖脂代谢紊乱机制研究

目的:探究黄精丸干预糖脂代谢紊乱的机制。方法:从中医药百科全书(ETCM)等数据库和相关文献搜集成分,根据胃肠吸收、P-糖蛋白底物和成药性模拟筛选活性成分,从SwissTargetPrediction等数据库搜集靶点,使用Cytoscape软件构建成分-靶点网络,并进行蛋白相互作用网络分析、GO功能富集和KEGG通路分析。结果:甾体皂苷类是黄精丸的主要活性成分,作用于由磷脂酰肌醇-3-激酶(PI3K)、信号转导和转录激活因子3(STAT3)等69个靶点构成的蛋白网络。靶点富集成蛋白质丝氨酸/苏氨酸激酶活动、PI3K-蛋白激酶B(PI3K-AKT)等通路。结论:网络药理学初步表明黄精丸调节糖脂代谢可能是通过甾体皂苷类成分,影响PI3K和STAT3等靶点,进一步影响蛋白质丝氨酸/苏氨酸活动、PI3K-AKT信号等通路。     

儿童CT扫描致癌风险评估研究进展

本文简要描述儿童CT扫描频度和受照剂量的发展趋势,主要介绍儿童CT扫描致癌风险评估在不同时期的主要方法及研究结果,阐述了儿童CT扫描的致癌风险,为儿童受照剂量的优化提供依据。     

The validation study of a novel assay with 30 slow and moderate mutation Y-STR markers for criminal investigation and database applications

International Journal of Legal Medicine - The Y chromosome short tandem repeat (Y-STR) haplotyping method has been widely used in forensic applications. However, the existing Y-STR panels are not...