甘草总黄酮氨水提取及超滤纯化工艺研究

目的 建立一种适合于工业化生产的甘草总黄酮提取和纯化方法。方法 以甘草总黄酮的提取率为指标,采用正交试验确定氨水提取的最佳条件;并以甘草总黄酮的转移率和除杂率为指标,采用正交试验优化最佳超滤工艺参数。结果 最佳提取条件：0.75%氨水24倍,提取3次,每次60 min,总黄酮平均提取率达83.5%;超滤结果表明：选用10 nm的无机陶瓷膜,在操作压力0.12 MPa和料液温度25 ℃条件下甘草总黄酮平均转移率和平均除杂率分别达到95.6%和23.3%。结论 该联用技术简便可行,生产成本低,适合工业化生产。     

《黄帝内经》眩晕证治探讨

认为《黄帝内经》中引起眩晕的病因病机主要有感受外邪、运气变化、脏腑内伤、经脉之气运行失常等四类,在经脉主要与足太阳、足厥阴、足少阳有关,在脏腑主要与脑、肝有关。《黄帝内经》对眩晕病证的治疗以针刺为主,偶有药物治疗,总的治疗原则是盛则泻之,虚则补之。      

针灸防治肿瘤相关性抑郁状态的临床实践指南研究

目的:探索针灸防治肿瘤相关性抑郁状态的临床实践方案,为制定临床实践指南提供依据。方法:人工检索49本古代医籍,收集关于针灸疗法治疗抑郁的古代文献证据;计算机检索国家知识基础设施数据库（CNKI）、中国医学生物文献数据库（CBM）、中国学术期刊数据库（CSPD）及中文科技期刊数据库（CCD）、Pubmed、EMBASE、Cochrane Library等数据库,全面收集针灸疗法治疗肿瘤相关性抑郁的现代文献证据;制定发放《针灸干预肿瘤相关性抑郁状态调查问卷》,收集针灸干预肿瘤相关性抑郁状态的现代专家经验。通过整合3方面证据形成针灸防治肿瘤相关性抑郁状态临床推荐方案。结果:古代医籍文献相关条目共14项;现代文献共检索到137篇文献,通过对文献进行纳入排除,最终纳入14篇现代文献,包括中文文献8篇,英文文献6篇;临床专家问卷共收回52份。综合3方面研究结果发现针灸治疗肿瘤相关性抑郁状态常用毫针针刺、电针、艾灸、耳穴贴压等疗法;常用穴位为神门、内关、印堂、百会、合谷、足三里、气海、三阴交、阴陵泉、太冲、肝俞、太溪、肾俞、心俞等。结论:综合3方面证据形成针灸防治肿瘤相关性抑郁状态的临床推荐应用方案,弱推荐使用毫针针刺、电针、艾灸、耳穴贴压疗法;推荐使用穴位为神门、内关、印堂、百会、合谷、足三里、气海、三阴交、阴陵泉、太冲、肝俞、太溪、肾俞、心俞等。     

集中隔离医学观察场所感染防控专家共识

 为科学指导新型冠状病毒肺炎(以下简称新冠肺炎, COVID-19)疫情期间集中隔离医学观察场所内感染防控工作, 在早期有效识别感染者的同时, 有效避免集中隔离医学观察场所内的交叉感染, 中华预防医学会医院感染控制分会、中国医院协会医院感染管理专业委员会、中国感染控制杂志编辑部组织有关专家共同制定本共识。本共识适用于确诊病例、疑似病例、无症状感染者的密切接触者、密切接触者的密切接触者, 以及入境人员或其他根据防控工作需要"应隔尽隔"人员开展集中隔离医学观察时所设置的集中隔离医学观察场所, 指导其工作人员正确做好个人防护及防止感染传播工作, 避免发生场所内感染及传播。本共识借鉴医疗机构内新型冠状病毒感染防控的基本原则、文件规范, 结合集中隔离医学观察场所的特点与实际工作情况, 对不同区域工作人员、工作环节开展新型冠状病毒传播的风险评估, 并据此对集中隔离医学观察场所的感染防控组织架构、选址布局、人员防护、人员闭环管理、清洁消毒、环境核酸监测、人员健康监测、职业暴露处理及交叉感染判定原则等感控相关工作给予指导。     

原发性甲状旁腺功能亢进性骨质疏松症的中医病机及防治思路

原发性甲状旁腺功能亢进症是引起骨质疏松症的较常见病因,原发性甲状旁腺功能亢进症是由一个或多个甲状旁腺病理性(肿瘤或增生)过量分泌甲状旁腺激素所造成.随着血钙测定和甲状旁腺素测定在临床上的广泛运用,甲状旁腺功能亢进性骨质疏松的患病率也随之上升,目前我国原发性甲状旁腺功能亢进症多为骨型和肾型.治疗方面,主要以手术治疗和药物...     

基于多靶点分子对接初探宣肺化浊方治疗新型冠状病毒肺炎的物质基础

目的:运用分子对接、靶点反向预测及反向分子对接技术探索宣肺化浊方治疗新型冠状病毒肺炎(COVID-19)的物质基础及可能的分子机制。方法:在中药系统药理学分析平台(TCMSP)检索宣肺化浊方中10味中药化合物,以血管紧张素转换酶2(ACE2),白细胞介素-6受体(IL-6R)为靶蛋白,运用分子对接筛选方中与ACE2,IL-6R对接打分较好且符合类药性的化合物,并进行化学信息学层次聚类分析;利用Swiss Target Prediction预测化合物潜在靶点;运用Cytoscape构建化合物-靶点网络;利用STRING分析靶点蛋白互相作用。结果:宣肺化浊方中针对ACE2潜在有活性的化合物共312个,活性较强的化合物15个;针对IL-6R符合条件的活性化合物共100个,活性较强的化合物3个,对活性较强的化合物进行化学信息学层次聚类分析发现大多属于黄酮类。药味-有效成分-靶点网络包含药材10味、化合物126个、靶点130个;STRING分析显示磷脂酰肌醇3激酶调节亚基1(PIK3R1),肉瘤基因(SRC),丝苏氨酸蛋白激酶1(AKT1),雄激素受体(AR)和表皮生长因子受体(EGFR)等蛋白可能是宣肺化浊方起效的关键靶点。结论:本研究基于多靶点分子对接虚拟筛选初步得到宣肺化浊方抗病毒、抗炎的物质基础,同时结合靶点反向预测和分析探索该方治疗COVID-19潜在作用靶点和分子机制,为宣肺化浊方及其相关方药的多角度挖掘和单体成分的现代化开发提供线索。     

A beneficial role of GLP-1 receptor agonist therapy in ABCC8-MODY (MODY 12)

Maturity-onset diabetes of the young (MODY) is an inherited form of diabetes resulting from a mutation in a single gene. ABCC8-MODY is caused by mutations in the ABCC8 gene, which encodes sulfonylurea receptor 1 (SUR1), a regulatory component of the ATP-sensitive potassium (K_ATP) channel found in beta cells. In ABCC8-MODY, mutations in the ABCC8 gene interfere with insulin secretion in response to glucose. Recent evidence suggests that therapy with GLP-1 receptor agonists (GLP-1 RAs) may be beneficial in ABCC8-MODY. This report presents a successful treatment of a 49-year-old woman diagnosed with ABCC8-MODY using the GLP-1 RA semaglutide. The patient, who had been previously receiving insulin therapy, experienced significant improvements in glycemic control and weight loss after transitioning to semaglutide. GLP-1 RAs potentially enhance insulin secretion in ABCC8-MODY by activating multiple signaling pathways involved in insulin secretion. The report highlights the potential of GLP-1 RA therapy as an alternative to sulfonylureas and insulin for individuals with ABCC8-MODY. GLP-1 RAs have previously demonstrated benefits in other forms of MODY. Understanding the molecular mechanisms through which GLP-1 RAs promote insulin secretion, including their effects on KATP channels and activation of PKA and Epac signaling, offers valuable insights into their therapeutic effects.     

Adipose-derived stem cell and their extracellular vesicles ameliorates immune function,and cardiac markers in experimental model of cardiorenal syndrome type III: TNF-α, IFN-γ and IL-10 cytokine production and their correlation with genotype

Cardio-renal syndrome (CRS) denotes the convergence of heart-kidney interactions across several mechanisms. The current study is conducted to evaluate the anti-inflammatory role of adipose tissue-derived stem cells (ASCs) versus adipose stem cell-derived extracellular vesicles (ADSCs-EVs) in experimental model of cardiorenal syndrome type III. The study was conducted on 50 male rats that were equally divided to: group I (control group); Group II (experimental cardiorenal syndrome group) which induced by right renal artery ligation (ICRSIII); Group III (Sham-operated control group) which underwent surgical incision without renal artery ligation; Group IV (ICRSIII which received ADSCs-extracellular vesicles (ADSCs-EVs); Group V (ICRSIII which received adipose tissue stem cells (ASCs). Assessment of pro-inflammatory cytokines; IL-10, IL-1α, IL-6, IL-1 β, IFN-γ, NF-α and their mRNA gene expression quantitation, (NGAL), and brain natriuretic peptide (BNP) as markers of cardiac dysfunction, as well as histopathological examination of renal tissue was examined by H& E, Masson trichrome and periodic acid-Schiff stains (PAS). The ICRS group exhibited significant acute tubular injury with tubular dilation, loss of brush borders, epithelial flattening, and occasional sloughed cells in lumen. Use of either ADSCs-EVs or ASCs significantly ameliorated the histological findings of tubular injury. Proinflammatory cytokines, BNP and NGAL were significantly elevated in ICRSIII group as compared to all other studied groups. Administration of ADSCs-EVs or ASCs led to significant decrease in all proinflammatory cytokines as well as BNP and NGAL levels with no significant difference between them. In conclusion, ADSCs-EXs and ASCs exhibited significant repairing effects in experimental-induced cardiorenal syndrome type III as evidenced by amelioration of histological findings of tubular injury, anti-inflammatory effects, and the significant decrease in markers of cardiac dysfunction. ADSC-EVs reprogramed injured cardiac cells by activating regenerative processes.     

中药通过调控短链脂肪酸防治骨质疏松症的研究进展

肠道菌群微生物是与宿主共生的一个大的生物群落，当其发生紊乱时将会导致多种疾病的发生，尤其是全身代谢性疾病。其代谢产物也对全身代谢性疾病起到重要的调控作用，尤其是骨代谢性疾病。短链脂肪酸(short chain fatty acids, SCFAs)是肠道微生物群所产生的一类代谢产物，能够很好地反映肠道菌群微生物的功能，大量的研究证明其在骨代谢性疾病中起着调控作用。中药在调控肠道菌群微生物及其代谢产物中有很大的作用，尤其是对于SCFAs的调控。笔者就单味中药及提取物与中药复方通过调控SCFAs影响骨代谢，从而在防治骨质疏松症方面的研究进行总结，以期为骨质疏松症的预防和治疗提供新思路。