锰苯甲酸卟啉在嘌呤霉素氨基核苷诱导的大鼠肾脏病模型中的作用

目的:探讨超氧化物歧化酶类似物锰苯甲酸卟啉(MnTBAP)在嘌呤霉素氨基核苷(puromycin aminonucleoside,PAN)诱导的大鼠肾脏病模型中的作用?方法:24只雄性SD大鼠按照每组8只随机分为正常对照组?模型组(一次性尾静脉注射150 mg/kg PAN建立PAN诱导的大鼠肾脏病模型)?MnTBAP治疗组[造模大鼠于造模当天开始腹腔注射MnTBAP 10 mg/(kg·d),共给药14 d]?收集大鼠24 h尿液,Bradford法检测24 h尿蛋白总量;应用透射电镜观察肾小球足细胞的超微结构;实时荧光定量PCR法在mRNA水平检测足细胞裂孔隔膜蛋白Nephrin和Podocin的表达量;Western blot在蛋白质水平检测Nephrin和Podocin的表达量?结果:①与正常对照组相比,模型组大鼠的24 h尿蛋白总量明显升高(P < 0.01),肾脏指数升高(P < 0.01);MnTBAP治疗后大鼠24 h尿蛋白总量较模型组下降(P < 0.01),肾脏指数较模型组下降(P < 0.01);②透射电镜观察结果表明,模型组大鼠的肾小球足细胞足突出现广泛融合甚至消失,MnTBAP治疗后这种现象得以改善;③实时荧光定量PCR结果显示,模型组大鼠肾皮质Nephrin和Podocin的mRNA表达较正常对照组降低(P < 0.01),MnTBAP治疗组Nephrin和Podocin的mRNA表达较模型组增加(P < 0.01);④Western blot结果显示,模型组大鼠肾皮质Nephrin和Podocin蛋白的表达较正常对照组降低(P < 0.01),MnTBAP治疗组Nephrin和Podocin蛋白的表达较模型组增加(P < 0.01)?结论:MnTBAP可以降低PAN诱导的肾病大鼠的蛋白尿,并明显减轻足细胞损伤?     

雷公藤甲素通过调控TLR/NF-κB信号通路对早期糖尿病肾病足细胞的作用研究

目的 观察雷公藤甲素治疗糖尿病肾病小鼠的疗效,并探讨其通过Toll-样受体（TLR）/核因子κB（NF-κB）信号通路对糖尿病肾病足细胞的作用机制。方法：9周龄db/m和db/db雄性小鼠分为db/m正常对照组（等量0.9%生理盐水）、db/db糖尿病对照组（等量0.9%生理盐水）、替米沙坦治疗组[5mg /( kg.d)]、雷公藤甲素组 [50ug /( kg.d) ],治疗8周。记录各组小鼠一般情况、24h尿蛋白定量、体质量、肾脏指数和血生化指标。光镜观察肾小球病变情况。WT1免疫组化计算足细胞数量。Western blot检测肾脏足细胞相关蛋白Nephrin、Podocin以及肾脏组织TLR4和NF-κB蛋白定量表达。结果 与糖尿病对照组比较,经雷公藤甲素组小鼠治疗后24h尿蛋白定量[(98.28±11.10)mg/24h比(239.89±44.26)mg/24h]、血清肌酐[(38.82±3.66)μmol/L 比(45.32±7.63)μmol/L]、总胆固醇[(3.05±0.54)mmol/L比(4.00±0.67)mmol/[及甘油三酯[(1.23±0.15)mmol/L比(2.09±0.48)mmol/L]均明显下降(P 均< 0.01),血清白蛋白水平上升[(39.21±4.33)g/L比(25.46±1.54)g/L,P < 0.01],肾小球肥大和足细胞损伤减轻,Nephrin[ (2.05±0.44)比(1.15±0.22)]、Podocin[(1.32±0.25)比(0.72±0.20)]蛋白表达量上升;(P 均< 0.01),TLR4[ (0.37±0.04)比(0.61±0.04)]和NF-κB[ (0.41±0.05)比(0.76±0.06)]蛋白表达量下降(P 均< 0.01)。结论 雷公藤甲素可能通过抑制TLR/NF-κB信号通路相关因子的表达,减轻糖尿病肾病小鼠足细胞损伤,减低蛋白尿,保护肾功能,起到治疗糖尿病肾病的作用。     

ROS介导棕榈酸诱导的肾小球足细胞骨架及裂孔隔膜损伤

目的：探讨棕榈酸（palmitic acid,PA）对足细胞肌动蛋白纤维骨架及裂孔隔膜蛋白损伤的影响。方法：应用PA刺激体外培养的条件永生性小鼠肾小球足细胞株,分为对照组（1% BSA）和PA（150 ?滋mol/L）组。采用Alexa 549-phalloidin染色观察足细胞肌动蛋白纤维骨架的变化;免疫荧光染色法观察检测足细胞裂孔隔膜蛋白Nephrin和Podocin的表达;油红“O”、透射电镜以及BODIPY染色检测PA刺激下足细胞内脂质积聚情况。应用不同浓度PA刺激足细胞,分为对照组（1% BSA）、50 ?滋mol/L PA组、150 ?滋mol/L PA组、300 ?滋mol/L PA组。采用Western blot检测足细胞裂孔隔膜蛋白Nephrin和Podocin的表达。进一步应用氧自由基清除剂N-乙酰半胱氨酸（N-acetyl cysteine,NAC）预处理足细胞,分为对照组（1% BSA）、PA（150 ?滋mol/L）组、NAC组、PA（150 ?滋mol/L）+ NAC组。采用DCFH-DA染色检测细胞内活性氧（reactive oxygen species,ROS）产生;Alexa 549-phalloidin染色观察足细胞肌动蛋白纤维骨架的变化;Western blot检测足细胞裂孔隔膜蛋白Nephrin和Podocin的表达。结果：Western blot结果示300 ?滋mol/L PA组足细胞内Nephrin和Podocin 的表达（0.360±0.030,0.900±0.040）分别与对照（Ctr）组（1.000±0.030,1.000±0.030）和50 ?滋mol/L PA组（0.912±0.020,0.900±0.040）相比都明显减少（P=0.000,P=0.000;P=0.000,P=0.003）。免疫荧光显示与Ctr组相比,PA组足细胞Nephrin（t=6.014,P=0.010）和Podocin（t=6.171,P=0.010）蛋白的表达减少。使用NAC预处理足细胞清除ROS,与PA组（1.21±0.04,1.30±0.03）相比,PA+NAC组Nephrin（2.87±0.05）和Podocin（2.69±0.06）的表达减少（P=0.000,P=0.000）。结论：ROS介导了棕榈酸诱导的足细胞骨架及裂孔隔膜损伤。     

Kindlin-2介导足细胞损伤在局灶节段肾小球硬化症模型小鼠发病中的作用研究

目的探讨Kindlin-2介导足细胞损伤在局灶节段肾小球硬化症模型小鼠发病中的作用。方法 8周龄雄性BALB/C小鼠分为正常对照组和模型组,各18只。模型组小鼠注射阿霉素(ADR,浓度2mg/m L,剂量10mg/kg鼠重)造模,正常对照组小鼠给予等量的生理盐水,于第4、8、12周每组各处死6只小鼠,检测24h尿蛋白定量、血清白蛋白(ALB)、总胆固醇(TC),光镜观察肾脏组织病理学改变,免疫组织化学技术检测足细胞数量,RT-q PCR技术检测肾脏组织Kindlin-2、肾病蛋白(Nephrin)及Podocin m RNA表达水平。结果与正常对照组比较,模型组4、8、12周24h尿蛋白定量、血总胆固醇(TC)均增加[24h尿蛋白定量:(5.66±1.06)mg/24h比(1.72±0.62)mg/24h、(4.63±1.16)mg/24h比(1.77±0.63)mg/24h、(4.78±0.87)mg/24h比(1.69±0.49)mg/24h;血TC:(8.84±1.30)mmol/L比(2.01±0.49)mmol/L、(7.45±1.27)mmol/L比(2.06±0.49)mmol/L、(6.79±1.38)mmol/L比(2.10±0.54)mmol/L,P均0.05];血ALB水平降低[(11.98±1.33)g/L比(22.27±1.41)g/L、(12.78±1.47)g/L比(22.65±1.49)g/L、(12.99±1.38)g/L比(23.31±1.49)g/L,P均0.05];模型组第4周可见部分肾小球出现局灶、节段硬化,伴有足细胞肿胀;第8周部分肾小球出现球性硬化,伴有明显的足细胞增生和肥大;第12周弥漫性肾小球出现球形硬化。模型组足细胞数量随着时间的进展逐渐减少[(77.40±7.56)个/肾小球比(104.00±10.05)个/肾小球,(63.27±8.51)个/肾小球比(103.80±12.23)个/肾小球,(53.15±8.23)个/肾小球比(98.86±8.89)个/肾小球,P均0.05]。模型组第4周开始Kindlin-2 m RNA水平明显上调[(1.14±0.06)、(1.13±0.08)、(1.45±0.10)比(0.82±0.07),P均0.05],Podocin m RNA水平明显下调[(1.79±0.08)、(1.64±0.06)、(1.45±0.07)比(2.05±0.05),P均0.05],第8周开始Nephrin m RNA水平明显下调[(1.42±0.13)、(1.17±0.11)比(2.06±0.20),P均0.05]。Kindlin-2 m RNA水平与足细胞数量(r=-0.02,P0.01)、Nephrin m RNA水平(r=-0.8,P0.01)、Podocin m RNA水平(r=-0.9,P0.01)呈显著负相关。结论 Kindlin-2可能通过介导足细胞损伤参与小鼠局灶节段肾小球硬化症的发病过程。     

SRT1720对DOCA盐敏感性高血压大鼠的治疗作用

目的:观察沉默信息调节因子2相关酶1(Sirtuin1,SIRT1)激活剂SRT1720对醋酸去氧皮质酮(DOCA)盐敏感性高血压大鼠(DHR)血压的调控作用?方法:28只SD大鼠行左肾切除后随机分为3组:对照组?模型组?SRT1720治疗组?对照组(n=8):饮自来水;模型组(n=10)和SRT1720治疗组(n=10):将200 mg/kg DOCA与硅胶制成的橡皮介质放置在大鼠两侧肩胛骨之间的皮下,并予1% NaCl饮水?SRT1720治疗组给予100 mg/(kg·d)灌胃?每周测收缩压1次?4周后处死,处死前检测中心动脉压及大鼠脉搏波速度,处死后取胸主动脉分别作HE染色观察主动脉厚度与血管口径变化?结果:模型组大鼠收缩压?中心动脉压?脉搏波速度明显增高,HE染色示血管平滑肌细胞(VSMC)明显肥大,弹力纤维层增厚,主动脉厚度显著增厚,而血管内径则明显下降?SRT1720治疗组大鼠收缩压?中心动脉压及脉搏波速度均较模型组显著下降?同时,SRT1720治疗后血管壁的病理改变明显减轻,血管平滑肌细胞肥大受到显著抑制,主动脉厚度显著下降,而血管内径明显增加?结论:SRT1720可通过抑制血管平滑肌细胞肥大,降低主动脉厚度,从而扩大血管内径,降低中心动脉压及收缩压?     

1,25(OH)_2D_3对嘌呤霉素氨基核苷酸肾病大鼠足细胞损伤的保护作用

目的 观察1,25(OH)2 D3 对嘌呤霉素氨基核苷酸肾病(PAN)大鼠足细胞损伤的保护作用并探讨可能的作用机制.方法 72只SD大鼠随机分为正常对照组(NC)、PNA组(PAN)和1,25(OH)2D3治疗组(T).一次性尾静脉注射PAN 100 mg/kg体重建立PAN肾病动物模型.于3、7、14、21d分批处死动物,分别检测不同时间点24 h尿蛋白和肾功能,PAS染色和透射电镜观察肾组织学改变,RT-PCR、免疫组化、western-blot分别检测nephrin,BMP-7,p-Smau1/5/8的表达.结果 (1)PAN大鼠24 h尿蛋白、尿素氮、血肌酐均高于同期的NC组,且足细胞足突增宽融合;T组各时间段24 h尿蛋白和肾功能较PAN组显著降低(P＜0.05),肾脏病理改变减轻.(2)与NC组比PAN大鼠nephrin表达减少并由正常的沿毛细血管襻线状分布向颗粒状、团快状改变.T组大鼠nephrin的表达显著增强且保持着正常的沿毛细血管襻线状分布.(3)PAN组BMP-7 mRNA和蛋白的表达以及p-Smaa1/5/8的表达均低于NC组;T组BMP-7 mRNA和蛋白的表达以及p-smad1/5/8的表达显著升高.结论 1,25(OH)2 D3能有效的抑制嘌呤霉素氨基核苷酸诱导的足细胞损伤,减少蛋白尿;1,25(OH)2D3对足细胞损伤的保护作用可能与调控BMP-7信号的活化有关.     

1,25(OH)2D3对嘌呤霉素氨基核苷酸肾病大鼠足细损伤的保护作用

目的 观察1,25(OH)2D3对嘌呤霉素氨基核苷酸肾病（PAN）大鼠足细胞损伤的保护作用并探讨可能的作用机制。方法 72只SD大鼠随机分为正常对照组（NC）、PNA组（PAN）和1,25(OH)2D3治疗组（T）。一次性尾静脉注射PAN 100mg/kg体重建立PAN肾病动物模型。于3、7、14、21d分批处死动物,分别检测不同时间点24h尿蛋白和肾功能,PAS染色和透射电镜观察肾组织学改变,RT-PCR、免疫组化、western-blot分别检测nephrin,BMP-7, p-Smad1/5/8的表达。结果①PAN大鼠24h尿蛋白、尿素氮、血肌酐均高于同期的NC组,且足细胞足突增宽融合;T组各时间段24h尿蛋白和肾功能较PAN组显著降低（P<0.05）,肾脏病理改变减轻。②与NC组比PAN大鼠nephrin表达减少并由正常的沿毛细血管襻线状分布向颗粒状、团快状改变。T组大鼠nephrin的表达显著增强且保持着正常的沿毛细血管襻线状分布。③PAN组BMP-7 mRNA和蛋白的表达以及p-Smad1/5/8的表达均低于NC组;T组BMP-7 mRNA和蛋白的表达以及p-Smad1/5/8的表达显著升高。结论 1,25(OH)2D3能有效的抑制嘌呤霉素氨基核苷酸诱导的足细胞损伤,减少蛋白尿; 1,25(OH)2D3对足细胞损伤的保护作用可能与调控BMP-7信号的活化有关。     

嘌呤霉素致足细胞损伤细胞模型的建立

目的:建立小鼠足细胞损伤的细胞模型,为进一步从细胞、分子水平研究足细胞的牛物学作用,以及足细胞特别是裂孔隔膜(slit diaphragm)分子在蛋白尿发生、发展中的分子机制提供稳定、可靠的足细胞损伤模型.方法:应用不同浓度的嘌呤霉素(15 ms/L、45 ms/L和75 ms/L)作用于足细胞,通过流式细胞仪检测作用24 h和48 h后足细胞的凋亡情况,以确定嘌呤霉索的作用浓度和时间,建立足细胞损伤的细胞模型;并以噻唑蓝(MTT)检测足细胞损伤后的活力,应用免疫荧光染色观察裂孔隔膜关键分子Nephrin、Podocin以及骨架蛋白F-actin在足细胞损伤中的分布来评价足细胞损伤.结果:45 mg/L嘌呤霉素作用48 h以及75 ms/L嘌呤霉素作用24 h或48 h,足细胞明显凋亡.结论:嘌呤霉素可以呈时间和剂量依赖件诱导小鼠足细胞凋亡,45 mg/L嘌呤霉素作用 48 h后诱导的足细胞损伤模型稳定、可靠,可应用于足细胞损伤的研究.     

坎地沙坦对糖尿病肾病大鼠足细胞Nephrin、Podocin和CD2AP表达的影响

目的探讨糖尿病肾病(DN)大鼠足细胞相关分子Nephrin、Podocin及CD2AP表达的变化,以及坎地沙坦对其的干预作用,为DN的防治提供理论依据。方法健康雄性SD大鼠36只,随机分为3组:A组为正常对照组,B组为DN组,C组为DN+坎地沙坦组。用药后第4周和第7周检测各组大鼠的血糖(GLU)、体重、24h尿蛋白(proteinuria)和内生肌酐清除率(Ccr),于第7周予RT-PCR法检测大鼠肾组织Nephrin、Podocin及CD2AP mRNA表达。结果 7周后,与A组比较,B组出现尿蛋白排泄增多,Ccr下降(P<0.05),肾组织Nephrin、Podocin及CD2AP mRNA水平均下调(均P<0.05);与B组比较,C组尿蛋白排泄量降低(P<0.05),Ccr升高(P<0.05),Nephrin、Podocin及CD2AP mR-NA水平均增加(均P<0.05)。采用直线相关分析后发现,尿蛋白与肾组织Nephrin、Podocin和CD2AP mRNA水平呈负相关;Ccr与肾组织Nephrin、Podocin和CD2AP mRNA水平呈正相关。结论 DN时存在足细胞分子Nephrin、Podo-cin及CD2AP表达异常,坎地沙坦对肾脏的保护作用机制可能是通过增加Nephrin、Podocin及CD2AP表达而实现。     

雷公藤甲素通过调控Toll-样受体(TLR)/核因子κB(NF-κB)信号通路对糖尿病肾病模型小鼠足细胞的作用研究

目的观察雷公藤甲素治疗糖尿病肾病(DN)模型小鼠的疗效,并探讨其通过Toll-样受体(TLR)/核因子κB(NF-κB)信号通路对DN足细胞的作用机制。方法6只9周龄db/m和18只9周龄db/db雄性小鼠,按db/m正常对照组(等量0.9%生理盐水)、db/db DN对照组(等量0.9%生理盐水)、替米沙坦治疗组(5mg·kg^-1·d^-1)、雷公藤甲素组(50μg·kg^-1·d^-1)分组给药,治疗8周。记录各组小鼠一般情况、24h尿白蛋白定量、体质量、肾脏指数和血生化指标,光镜观察肾小球病变情况,免疫组化计算足细胞数量,Western blot检测肾脏足细胞相关蛋白Nephrin、Podocin以及肾脏组织TLR4和NF-κB蛋白定量表达。结果与DN对照组比较,雷公藤甲素组小鼠治疗后24h尿蛋白定量[(98.28±11.10)mg/24h比(239.89±44.26)mg/24h]、血清肌酐[(38.82±3.66)μmol/L比(45.32±7.63)μmol/L]、总胆固醇[(3.05±0.54)mmol/L比(4.00±0.67)mmol/L]及三酰甘油[(1.23±0.15)mmol/L比(2.09±0.48)mmol/L]均明显下降(P均<0.01),血清白蛋白水平上升[(39.21±4.33)g/L比(25.46±1.54)g/L,P<0.01],肾小球肥大和足细胞损伤减轻[(95.50±5.01)个/肾小球比(58.78±2.86)个/肾小球,P<0.05],Nephrin[(2.05±0.44)比(1.15±0.22)]、Podocin[(1.32±0.25)比(0.72±0.20)]蛋白表达量上升(P均<0.01),TLR4[(0.37±0.04)比(0.61±0.04)]和NF-κB[(0.41±0.05)比(0.76±0.06)]蛋白表达量下降(P均<0.01)。结论雷公藤甲素可能通过抑制TLR/NF-κB信号通路相关因子表达,减轻糖尿病肾病模型小鼠足细胞损伤,减低蛋白尿,保护肾功能,起到治疗糖尿病肾病的作用。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.