大鼠全脑缺血再灌注后皮质和海马中ODC的表达

目的：检测大鼠全脑缺血再灌注不同时相皮质、海马中鸟氨酸脱羧酶（ODC）的表达，探讨脑缺血再灌注后ODC活性增高的机制。方法：采用改良的四血管关闭法复制大鼠全脑缺血再灌注2 h，4 h，6 h，8 h动物模型；用逆转录PCR（RT-PCR）方法检测不同时相皮质、海马标本中ODC mRNA的表达。结果：对照组大鼠皮质、海马中未检测出ODC mRNA的表达；实验组大鼠皮质、海马中检测出有ODC mRNA的表达，且不同时相的表达差异具有统计学意义（P<0.01）。结论：大鼠全脑缺血再灌注后皮质、海马中ODC mRNA表达明显增加，脑缺血再灌注后ODC活性明显增加可能是ODC mRNA表达增加、酶蛋白合成增多的结果。     

鸟氨酸脱羧酶与结直肠癌关系的研究进展

<正>多胺是调节细胞生长、发育和促进组织创伤愈合的细胞信号分子,鸟氨酸脱羧酶(ornithine decarboxylase,ODC)是多胺代谢过程中的第一限速酶,催化鸟氨酸脱羧生成腐胺,研究显示ODC活性和多胺合成在多种上皮癌中增加,其中包括皮肤癌、前列腺癌和结直肠癌。结直肠癌中K-ras基因突变和腺瘤性息肉病基因的突变诱导ODC基因表达,抑制多胺的代谢,ODC活性的增加与结直肠癌的发生与发展关系密切。在人类ODC     

加味丹参饮预处理调节ODC活性抗大鼠心肌缺血再灌注损伤研究

目的：探讨加味丹参饮通过调节鸟氨酸脱羧酶（ODC）活性在抗SD大鼠心肌缺血再灌注损伤（IRI）中的保护机制。方法建立SD大鼠心肌IRI模型,设对照组、假手术组、缺血再灌注损伤（IRI）组、加味丹参饮(JWDSY)+IRI组共4组。酶联免疫(ELISA)法测定ODC含量,实时荧光定量PCR（RT-PCR）方法检测ODC mRNA表达水平,免疫印迹（Western blot）法检测ODC蛋白表达水平,高效液相色谱检测心肌组织中多胺的含量。结果与对照组比较,假手术组的ODC含量、ODC mRNA及蛋白表达水平均无统计学意义（P＞0.05）;与假手术组比较,IRI组的ODC含量显著增加、ODC mRNA及蛋白表达水平显著升高,差异有统计学意义（P＜0.01）;与IRI组比较, JWDSY+IRI组ODC含量显著减少,ODC mRNA和蛋白表达水平显著降低,差异有统计学意义（P＜0.01）。结论加味丹参饮可能通过调节ODC活性,从而发挥对IRI后心肌的保护作用。     

鸟氨酸脱羧酶活性微量测定法

鸟氨酸脱羧酶(ODC,EC4,1,1,17)是多胺生物合成的限速酶,多胺在生物大分子的合成及细胞生长调节中起重要作用。ODC 活性在快速生长的细胞、再生组织及恶性增生的细胞中都有明显增加。已有资料证明促癌作用与 ODC 的诱导有关,并认为 ODC 活性测定可作为鉴定促癌物、筛选抗癌药及研究促     

鸟氨酸脱羧酶与胃癌

<正>在正常细胞周期中,细胞的生长、分化均受到某些酶的调控.鸟氨酸脱羧酶(Ornithime decarboxylase,ODC)广泛分布在动物体内,它是多胺(Polyamines)合成的关键酶.而多胺能够刺激DNA、RNA和蛋白质的合成,并且参与维持细胞膜及细胞骨架结构的稳定性.凡生长旺盛或迅速增生的组织,其ODC的活性和多胺的含量均增加.近年来研究发现ODC与肿瘤的关系十分密切.现就其与幽门螺杆菌感染及胃癌的关系概述如下.1 ODC的生物学作用1.1 ODC与多胺合成 ODC是一个胞浆酶,是以磷酸吡哆醛为辅因子的氨基酸脱羧酶.它与S一腺苷蛋氨酸脱羧酶(SAMDC)和精脒、精胺合成酶(spermidine/spermine synthase)共同调控多胺(包括腐胺、精脒和精胺)的合成,其中ODC被公认为是多胺合成的第一个酶     

过表达ODC抑制氧化应激诱导大鼠心肌细胞凋亡的研究

目的构建过表达鸟氨酸脱羧酶(ODC)基因慢病毒载体,并研究其对氧化应激诱导大鼠H9C2心肌细胞凋亡的影响。方法构建大鼠ODC基因pHIV-ODC过表达质粒,与包装质粒psPAX2、pMD2G共转染293FT细胞,检测其侵染效率;包装慢病毒并侵染H9C2心肌细胞;72 h后观察其侵染效率,RT-PCR法检测细胞ODC mRNA的表达,利用CCK-8、Hochest33258染色检测ODC对H2O2诱导H9C2心肌细胞凋亡的影响。结果酶切及测序鉴定ODC序列正确插入慢病毒过表达载体。侵染H9C2细胞72 h后ODC mRNA水平较阴性慢病毒感染组显著升高。过表达ODC能抑制H2O2诱导的细胞活力下降,减轻H2O2诱导的心肌细胞凋亡,与H2O2组比较差异均有统计学意义(P<0.001)。结论成功构建的过表达ODC慢病毒,上调H9C2细胞中ODC的表达,过表达ODC能显著抑制氧化应激诱导的H9C2心肌细胞凋亡。     

rAd-ODC/Ex3as对前列腺癌PC-3细胞抑制作用的研究

目的:探讨重组腺病毒rAd鄄ODC/Ex3as对前列腺癌细胞的体外抑制作用及对前列腺癌的作用机制。方法:利用报告基因GFP测定病毒感染效率,用WesternBlot检测rAd鄄ODC/Ex3as是否抑制鸟氨酸脱羧酶(ODC)基因的表达,采用MTT法、Matrigeal侵袭实验观察rAd鄄ODC/Ex3as对前列腺癌细胞PC鄄3恶性表型的影响,并用流式细胞仪检测细胞周期和凋亡。结果:当MOI为50时,腺病毒感染PC鄄3细胞的效率约为70%,WesternBlot证实rAd鄄ODC/Ex3as可抑制ODC基因的表达。rAd鄄ODC/Ex3as以20MOI感染PC鄄3细胞可明显抑制其生长增殖和侵袭性穴P<0.01雪,流式细胞仪检测结果证实rAd鄄ODC/Ex3as可引起PC鄄3细胞G1期阻滞,但并未引起凋亡。结论:rAd鄄ODC/Ex3as在体外能有效地干扰ODC基因的表达,并可抑制前列腺癌细胞PC鄄3的生长与侵袭,诱导其发生G1期阻滞,有望成为治疗前列腺癌的基因药物。     

卵巢癌组织中鸟氨酸脱羧酶基因过度扩增及其临床意义

目的检测鸟氨酸脱羧酶(ornithine decarboxylase,ODC)基因在卵巢癌组织、癌旁组织及卵巢良性肿瘤中的表达,探讨ODC过度扩增在卵巢肿瘤发生、发展中的作用。方法应用RT-PCR方法检测40例卵巢癌组织、15例癌旁正常卵巢组织、15例卵巢良性肿瘤中ODC基因的表达,比较ODC在不同卵巢组织中的阳性表达率。结果ODC在癌旁正常卵巢组织中表达率为26.67%,在15例卵巢良性肿瘤组织中均无表达,在卵巢癌组织中表达率为65%。统计学分析结果显示ODC在卵巢癌组织的表达明显高于癌旁正常卵巢组织和卵巢良性肿瘤,差异均有统计学意义(P<0.05);ODC在浆液性癌表达阳性率为83.33%,较其他类型卵巢恶性肿瘤的50.00%明显增加,差异有统计学意义(P=0.028)。在Ⅰ,Ⅱ期患者ODC阳性率为54.55%,其中强阳性2例;Ⅲ,Ⅳ期患者ODC阳性率68.97%,其中强阳性17例,两者相比差异无统计学意义(P=0.469),而强阳性率Ⅲ,Ⅳ期为58.62%明显高于Ⅰ,Ⅱ期的18.18%,差异有统计学意义(P=0.034)。在中、高分化癌组织中ODC强阳性表达率为20%,低分化癌组织中强阳性表达率为64%,差异有统计学意义(P=0.018)。在癌旁正常卵巢组织中ODC表达较卵巢良性肿瘤有增高趋势,但差异无统计学意义(P=0.10)。结论ODC过度扩增可能在卵巢癌的发生、发展中起重要作用。     

芳维A酸乙酯对小鼠皮肤乳头状瘤形成过程中ODC、PCNA的影响

目的 通过测定小鼠二阶段皮肤乳头状瘤模型中鸟氨酸脱羧酶（ODC）、增殖细胞核抗原（PCNA）的变化,探讨芳维A酸乙酯的癌化学预防作用机制。方法 ODC作用于^14C标记的鸟氨酸底物后释放^14CO2,通过测定^14CO2生成量反映标本中ODC活性;用免疫组化方法检测测本中PCNA表达情况。结果 芳维A组乙酯对肿瘤标志物ODC、PCNA有明显的抑制作用。结论 芳维A酸乙酯对ODC、PCNA的抑制作用是其预防小鼠乳头状瘤发生的重要机制,它具有肯定的癌化学预防作用。     

老年人肺癌组织中鸟氨酸脱羧酶基因表达及其临床意义

目的：从mRNA和蛋白质水平探讨ODC基因表达和老年人肺癌的关系，为肺癌基因诊断和基因治疗的研究提供理论依据。方法：分别应用RT PCR和免疫组化方法检测42例老年人肺癌组织和癌旁正常肺组织中ODC mRNA和ODC蛋白的表达，应用Western Blot检测肺癌组织中ODC表达，同时用MTT法观察不同转染组肿瘤细胞的增殖状况，探讨上述指标与肺癌临床病理学特性的关系。结果：RT PCR显示，老年人肺癌组织中ODC mRNA表达明显高于癌旁正常肺组织（P＜0.05），不同类型和分化程度肺癌组织中ODC mRNA水平差异无统计学意义（P＞0.05），Ⅲ期肺癌组织中ODC mRNA表达明显高于Ⅰ期+Ⅱ期（P＜0.05）；免疫组化显示，肺癌组织中ODC蛋白表达明显高于癌旁正常肺组织(P＜0.05);不同病理类型和分化程度肺癌组织中ODC蛋白表达未见明显差异（P＞0.05），Ⅲ期肺癌组织中ODC蛋白表达较Ⅰ期+Ⅱ期明显升高（P＜0.05）；Western Blot显示，肺癌组织中ODC蛋白表达明显高于癌旁正常肺组织；MTT法观察生长曲线检测数据表明，ODC的反义RNA表达载体转染组细胞的增殖明显比对照组细胞和空载体转染组细胞缓慢。结论：ODC表达增强与老年人肺癌发生、发展、浸润及转移有关，可作为判断肺癌生物学行为的参考指标; ODC的反义RNA表达载体对A 549肺癌细胞株的生长具有一定的抑制作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.