桂枝的化学成分研究

目的：对桂枝的化学成分进行系统研究。方法：利用各种色谱技术进行分离纯化，并根据理化常数和光谱解析（UV，IR，MS，NMR）鉴定各化合物结构。从桂枝95％乙醇浸膏的氯仿和乙酸乙酯部分中，共分离得到11个化合物。结果：分别鉴定为：肉桂酸（I）、2－甲氧基肉桂酸（Ⅱ）、1，4－二苯基－丁二酮（Ⅲ）、香豆素（Ⅳ）、β－谷甾醇（Ⅴ）、丁香醛（Ⅵ）、5α，8α－过氧化麦角甾醇（Ⅶ）、2－甲氧基苯甲酸（Ⅷ）、6β－羟基－4－烯－3－豆甾酮（Ⅸ）、原儿茶酸（Ⅹ）、胡萝卜苷（Ⅺ）。结论：化合物Ⅱ、Ⅲ、Ⅷ为首次从天然界分离得到，化合物Ⅶ为首次从高等植物中分离得到，化合物Ⅵ、Ⅸ为首次从本属植物中分离得到。     

网脉橐吾的化学成分研究

目的：从菊科橐吾属植物网脉橐吾Ligularia dictyoneura (Franch.)Hand.-Mazz。根中提取分离活性成分。方法：95％乙醇提取，硅胶柱层析，重结晶等。结果：从石油醚部分分离鉴定了5个化合物，其中3个（Ⅰ，Ⅲ，Ⅳ）为eremophilane(艾里莫芬烷）型倍半萜，1个（Ⅱ）为bakkenolide(蜂斗菜内酯）型倍半萜，4个倍半萜Ⅰ，Ⅱ，Ⅲ，Ⅳ分别为：7（11）－eremophilen-8-one,bakkenolide A,6β-hydroxyeremophil-7(11)-en-12,8α－olide,6β,8β-hydroxyeremophill-7(11)-en-12,8α-olide，另外1个化合物为β-谷甾醇（Ⅴ）。结论：5个化合物均为首次从该植物中分得。     

吉林乌头的化学成分研究

目的：研究草药吉林乌头的化学成分，探讨其活性物质基础。方法：采用乙醇提取、溶剂萃取、硅胶及氧化铝柱层析等方法从吉林乌头中分离化学成分，并通过化学和光谱法鉴定结构。结果：从吉林乌头的根和地上部分中共分得12个化合物，并用光谱方法鉴定了它们的结构。从根中分离得到的5个化合物分别为gigaconitine(1),8-acetylexcelsine(2),11-acetyl-1,19-epoxydenudatine(3),lepetine(4),denudatine(5),从地上部分（含花，叶和茎）分离得到的7个化合物分别为lepenine(6),akirane(7),excelsine(8),β－谷甾醇（β-sitosterol,9),槲皮素（quercetin,10),山柰酚（kaempferol,11)和芦丁（rutin,12)。结论：首次从该植物中分离得到了C19二萜生物碱（化合物1），化合物3，4，5，9，10，11，12均为首次从该植物中分离得到，化合物2，4，7，8的^13CNMR数据为首次报道。     

见血封喉树叶化学成分研究

目的：研究见血封喉树叶（Antiaris toxicaria Lesch.）的化学成分。方法：采用溶剂提取萃取法、柱色谱等方法分离纯化化学成分,通过理化性质、波谱学方法鉴定化合物结构。结果：从见血封喉树叶中分离得到3个化合物,分别鉴定为：壬二酸（1）、丁二酸（2）、香草酸（3）。结论：化合物1～3为首次从该植物中分离获得的已知化合物。     

远志的化学成分研究（I）

目的：研究中药远志Polygala tenuifolia根中的化学成分。方法：采用硅胶、Sephadex LH-20及ODS柱层析进行分离纯化，通过理化性质和光谱分析鉴定结构。结果：从中分离得到了5个化合物，分别鉴定为tenuifoliside B(I),3,4,5－三甲氧基肉桂酸甲酯（Ⅱ），polygalaxanthoneⅢ（Ⅲ），7-O-methylmangiferin(Ⅳ）和lancerin(V)。结论：化合物Ⅱ和Ⅳ为首次从远志属植物中分离得到，化合物V为首次从远志植物中分离得到。     

羽叶千里光中一个新的双黄酮

目的：对羽叶千里光（Senecio argunensis Turcz．）全草的化学成分进行研究。方法：应用柱层析色谱技术分离羽叶千里光全草提取物，根据理化性质和光谱数据鉴定各化合物结构。结果：分离鉴定了11个化合物，分别为8，8″-亚甲基5R．（3，3’，4’，5，7-五羟基黄酮）（1）、异鼠李素（2）、克氏千里光碱（3）、琥珀酸（4）、β-胡萝卜苷（5）、槲皮素、金丝桃苷、槲皮素-5-O-β-D-葡萄糖苷、千里光碱、千里光菲林碱和β-谷甾醇。结论：化合物1为新化合物，命名为8，8″-亚甲基双槲皮素，化合物2-5为首次从该植物中分得。     

蝉翼藤茎化学成分研究(Ⅱ)

目的：对蝉翼藤Securidaca inappendiculata的化学成分进行研究。方法：利用硅胶柱色谱和中压液相色谱方法对蝉翼藤茎的95％乙醇提取物进行分离；采用UV，IR，MS，1D和2DNMR等技术对所得化合物进行结构研究。结果：分离鉴定了6个化合物，分别为：4，4’－二甲基－1，7－庚二酸（I），肌醇（Ⅱ），豆甾醇（Ⅲ），维太菊苷（Ⅳ），鼠李糖（V）和蔗糖（Ⅵ）。结论：I为首次从该植物中分离得到的化合物，其他化合物均为首次从本属植物中分离得到。     

广东桑种子的化学成分

目的：研究广东桑（Morus atropurpurea Roxb．）种子中的化学成分。方法：运用各种色谱方法进行分离纯化，根据理化性质和波谱数据鉴定化舍物的结构。结果：从广东桑种子中分离得到10个化合物，分别鉴定为β-蜕皮松（1）、无羁萜（2）、熊果酸（3）、柚皮苷（4）、柚皮素（5）、山柰酚（6）、槲皮素（7）、苯甲酸1-O-β-D-吡喃葡萄糖苷（8）、苯甲酸（9）及β-谷甾醇（10）。结论：化合物1～10均为首次从该植物中分离得到。     

大黄藤化学成分研究

目的：研究大黄藤（ Pierre）中的化学成分。方法利用正相硅胶柱色谱、半制备型高效液相色谱、葡聚糖凝胶及反相中压液相色谱等现代分离技术进行分离,根据理化性质和NMR,MS等方法鉴定其结构。结果从中分离得到8个化合物,分别是黄藤内酯（1）,马克甾酮 A（2）,环阿屯醇（3）, feruloylphenethylamine （4）,反式阿魏酰酪胺（5）,β-谷甾醇（6）,1-棕榈酸单甘油酯（7）,1-sinapoyl-β-D-glucopyranoside （8）。结论化合物2～8为首次从该植物中分离得到。     

民族药紫红獐牙菜中齐墩果酸和芒果甙的分离鉴定

目的：提取分离和鉴定民族药紫红獐牙菜的主要化学成分。方法：采用加压硅胶柱层析等方法进行提取分离，用紫外、红外、核磁、质谱四种光谱及有关理化性质进行分析鉴定。结果：从紫红獐牙菜的乙醇提取物中分得１３个单体化合物，化合物Ⅰ为齐墩果酸（Ｏｌｅａｎｏｌｉｃａｃｉｄ），Ⅱ为芒果甙（Ｍｅｎｇｉｆｅｒｉｎ），其余化合物正在鉴定中。结论：首次报道从该植物中分离鉴定出化合物Ⅰ和Ⅱ。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.