Wegener肉芽肿一例

郝某,女,30岁.1989年11月8日,右眼红磨反复发作一个月而来诊,诊断“角、巩膜炎”收入病房。既往史:半年前做过鼻息肉摘除术。查体:一般状态尚可,结膜囊分泌物涂片、菌培养均未查到致病菌。眼部检查:视力右:0.1,左0.8,右眼球突出,上睑红肿,结膜囊     

预注阿曲库铵对神经肌肉传导及呼吸功能的影响

预注阿曲库铵对神经肌肉传导及呼吸功能的影响彭志勇，曾邦雄，刘俊杰同济医科大学附属协和医院麻醉科，武汉４３００２２关键词肌松弛剂；阿曲库铵；呼吸中图法分类号Ｒ９７１，Ｒ３３２诱导前预注小剂量阿曲库铵（ａｔｒａｃｕｒｉｕｍ）可加快气管插管时间。此时，虽然...     

Ki67基因短发夹状RNA表达载体对肾癌细胞生长的抑制作用

目的研究针对Ki67基因的短发夹状RNA(shRNA)表达载体pSilencer-Ki67对人肾癌细胞生长的抑制作用。方法将pSilencer-Ki67转染人肾癌786-0细胞,24、48、72、120、144 h后分别采用逆转录-聚合酶链反应(RT-PCR)、Western blot技术检测Ki67 mRNA、蛋白表达,噻唑蓝(MTT)比色法检测细胞增殖,原位末端标记法检测细胞凋亡。结果pSilencer-Ki67转染后48 h 786-0细胞Ki67mRNA表达(34．1±1．7)%、蛋白表达(42．6±1．7)%降低最明显,与空质粒对照组[(97．7±0．9)%、(97．3±1．6)%]比较差异均有统计学意义(P＜0．01)。pSilencer-Ki67处理后48 h细胞增殖抑制率(76．7±3．2)%最高,72 h凋亡细胞阳性率(74．3±5．9)%最高,与空质粒对照组[(7．0±0．5)%、(8．4±1．1)%]比较差异有统计学意义(P＜0．01)。pSilencer-Ki67的这些作用可持续144 h。结论pSilencer-Ki67能有效、持续抑制人肾癌786-0细胞生长,有望成为肾癌基因治疗的有效工具。     

表达端粒酶逆转录酶siRNA的溶瘤腺病毒对裸鼠肾癌移植瘤的治疗作用

Objective To investigate the antitumor effect of oncolytic adenovirus armed with small interference RNA targeting hTERT gene for renal cancer therapy. Methods Nude mice were divid-ed randomly into 4 groups (8 mice/group),and were treated by intratumoral injections of ZD55-hTERT ( an oncolytic adenovirus armed with small interference RNA targeting hTERT gene) ,ZD55-EGFP ( an on-colytic adenovirus) and Ad-hTERT (replication-defective adenovirus armed with small interference RNA targeting hTERT gene) with three consecutive daily at 7 × 108 pfu/day or treated with PBS as a control. The expression of E1A and hTERT, and apoptosis of tumor xenografts were assessed by immunohistochemi-cal technique at the 7th day after injections. The tumor volume was measured at the 50th day after injec-tions. Results The tumor volume in ZD55-hTERT treatment group ( 124.1±27.5) was significantly less than that in ZD-EGFP (499.8±77.1 ) and Ad-hTERT ( 609.0±102.5 ) treatment groups. The E 1A pos-itive expression in ZD55-hTERT treatment group was significantly higher than that in Ad-hTERT treatment group. The hTERT positive expression in ZD55-hTERT treatment group was significantly lower than that in Ad-hTERT treatment group. ZD55-hTERT treatment of tumor xenografts resulted in an increased apoptotie cell death as compared with ZD55-EGFP and Ad-hTERT treatment. Conclusion The antitumor effect of ZD55-hTERT was more potent than oneolytie adenovirus ZD55-EGFP and Ad-hTERT.     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

中心静脉导管腹腔置管联合治疗肝硬化难治性腹水30例

我科自2000年4月～2003年8月，共收治肝硬化腹水患者592例，并发难治性腹水者58例，占9．8％。其中30例在保肝、限水、限钠、提高胶体渗透压等综合治疗的基础上，给予经腹腔留置中心静脉导管并向腹腔内注射多巴胺、呋噻米治疗，取得满意疗效，现总结如下。     

还原型谷胱甘肽治疗乙醇性肝炎40例疗效观察

由于乙醇成瘾，且人们对其危害认识不足，故酗酒者为数不少。长期大量饮酒超过机体对乙醇在肝内代谢能力，可引起乙醇性肝炎。2003年6月-2004年6月，我科采用还原型谷胱甘肽治疗乙醇性肝炎40例，疗效满意，现报告如下。     

流行性出血热多尿期少见并发症

流行性出血热多尿期的常见并发症有水、电解质紊乱,继发感染和肾衰等,然而有些并发症,虽不常见,但往往比较严重,甚至导致病人死亡。这些少见并发症主要有颅内出血、腔道出血、中毒性精神病、Flsher综合征、尿潴留、溃疡性角膜炎;高血钾、高渗性脱水、肠系膜血栓形成、高血压等。     

阿曲库铵气管插管肌松条件的研究

研究了肌松监测下河曲库铵气管插管的肌松条件。３２例患者随机分为三组。麻醉诱导：硫喷妥钠５～７ｍｇ／ｋｇ、阿曲库铵０．６ｍｇ／ｋｇ。根据肌松监测分别当Ｔ１＝２５％（组Ａ）、Ｔ１＝１０％（组Ｂ）、Ｔ１＝２％（组Ｃ）时气管插管。结果发现阿曲库铵首量后５．４分钟，Ｔ１＝２％时气管插管最为满意，随Ｔ１值的增加或给药后的时间缩短，插管的满意程度降低，尽管Ｔ１＝２５％时也可顺利气管插管，但插管的满意程度明显比Ｔ１＝２％时为差。三组气管插管后ＨＲ、ＭＡＰ均增加，但以组Ａ和组Ｂ增加明显，三组ＨＲ的增加幅度随Ｔ１值的增加而明显增加。     

基本功在临床诊断中的重要性

基本功在临床诊断中的重要性承德石油高等专科学校医院刘俊杰在临床诊断技术和设备日新月异的今天，疾病诊断的准确率有了明显的提高，有些难以早期诊断的疾病，可以通过特殊检查而获得早期诊断，早期治疗。但在医学飞跃进步的同时，也出现了另外一种令人担忧的问题，即忽...