表达端粒酶逆转录酶siRNA的溶瘤腺病毒对裸鼠肾癌移植瘤的治疗作用

Objective To investigate the antitumor effect of oncolytic adenovirus armed with small interference RNA targeting hTERT gene for renal cancer therapy. Methods Nude mice were divid-ed randomly into 4 groups (8 mice/group),and were treated by intratumoral injections of ZD55-hTERT ( an oncolytic adenovirus armed with small interference RNA targeting hTERT gene) ,ZD55-EGFP ( an on-colytic adenovirus) and Ad-hTERT (replication-defective adenovirus armed with small interference RNA targeting hTERT gene) with three consecutive daily at 7 × 108 pfu/day or treated with PBS as a control. The expression of E1A and hTERT, and apoptosis of tumor xenografts were assessed by immunohistochemi-cal technique at the 7th day after injections. The tumor volume was measured at the 50th day after injec-tions. Results The tumor volume in ZD55-hTERT treatment group ( 124.1±27.5) was significantly less than that in ZD-EGFP (499.8±77.1 ) and Ad-hTERT ( 609.0±102.5 ) treatment groups. The E 1A pos-itive expression in ZD55-hTERT treatment group was significantly higher than that in Ad-hTERT treatment group. The hTERT positive expression in ZD55-hTERT treatment group was significantly lower than that in Ad-hTERT treatment group. ZD55-hTERT treatment of tumor xenografts resulted in an increased apoptotie cell death as compared with ZD55-EGFP and Ad-hTERT treatment. Conclusion The antitumor effect of ZD55-hTERT was more potent than oneolytie adenovirus ZD55-EGFP and Ad-hTERT.     

艾滋病36例误诊分析

艾滋病是由人类免疫缺陷病毒引起的致命性慢性传染病，基本特征是CD4T淋巴细胞的耗竭，导致一系列免疫缺陷综合征。它可引起多系统的病变，且由于缺乏特异性体征，容易误诊。1998～2002年，共收治被误诊为艾滋病36例     

浅谈药物滥用现象

随着医药技术水平的不断提高，抗生素应用种类日益增多，但各级各类医院普遍存在着抗生素滥用现象，有些医生以发热作为抗生素治疗的指征，对病毒、寄生虫、真菌感染、恶性肿瘤、血液病等引起的发热也使用抗生素；未严格掌握预防用药指征，无菌手术也使用抗生素，或术前应用过早，术后停药过晚；抗生素剂量过大，疗程过长，多种联用等，不仅浪费，还可导致耐药性，严重影响抗生素的疗效，     

谷胱甘肽治疗抗结核药物所致肝损害53例分析

药物性肝损害是抗结核药物最常见的不良反应之一,也是结核患者停止化疗的主要原因.我科自1999年12月～2002年12月共收治肺结核患者396例,因抗结核药物所致肝损害98例,占24 7%,对其中53例采用谷胱甘肽治疗,疗效显著.……     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

中西医结合治疗少年原发性帕金森综合征一例

中西医结合治疗少年原发性帕金森综合征一例刘俊杰患者，男，１４岁，学生，１２岁时（１９８５年８月），无原因地自觉两腿软弱无力，走路不稳，跑步时尤甚。三月后，病情渐加重，逐出现手抖，执笔困难，书写字迹弯曲，而且越写越小，致辍学。半年后出现头后昂，双目紧闭...     

颈椎病手术的麻醉管理

本文报道２３例颈椎病手术患者采用１％普鲁卡因与２％利多卡因混合液局麻加静脉哌替啶强化取得满意的效果。文中围绕呼吸管理问题对前、后手术入路的麻醉用药、输血、补液及头部固定等注意事项均作了具体介绍。     

不同程度的急性失血对犬冠脉循环及心肌代谢的影响

采用电磁血流仪测量冠脉血流量和心输出量，以健康犬失血前后自身对照的方法，研究急性失血10％、20％和30％对冠脉循环及心肌代谢的影响。1材料与方法选健康杂种犬10只，基础麻醉后用麻醉机控制呼吸，左、右股动、静脉分别插管后供测压及放血用。经左颈外静脉插入7FSwan-Ganz漂浮导管供测肺动脉压及肺毛细血管楔压。以上压力均用RM-6000型生理多导仪（日本NihonKonden）测试。开胸后用自制的14～16F塑料导管将冠状窦血流引至右颈静脉回输体内，用MFV-1200型电磁血流仪（日本NihonKonden）测量冠状窦引流血量及心输出量。右股动脉插管…     

亚低温在重症急性脑卒中的临床应用

目的　探讨亚低温治疗技术治疗重症急性脑卒中的临床疗效。方法　6 0例急性脑卒中患者随机分为亚低温治疗技术治疗组30例和对照组30例,对照组仅予常规神经科药物治疗,治疗后1个月进行神经功能缺损评分。结果　治疗组治疗后神经功能缺损评分改善明显好于对照组,两组比较有显著性差异(P <0 .0 5 ) ,治疗组的病死率6 . 7% ,对照组的病死率2 6 . 7%。结论　亚低温治疗技术明显促进重症急性脑卒中患者的神经功能恢复,降低死亡率。