五柱五桠果的化学成分及其体外抗肿瘤抗缺氧活性

目的 研究五柱五桠果的化学成分及其抗肿瘤抗缺氧活性。方法 利用各种色谱技术分离化学成分,根据理化性质与光谱数据鉴定结构,利用MTT法测试抗肿瘤活性。结果 从五柱五桠果果实中分离鉴定了白桦酸 (1)、白桦醇 (2)、羽扇豆醇 (3)、谷甾醇 (4)、没食子酸 (5)、没食子酸乙酯 (6)、原儿茶酸 (7)、原儿茶酸甲酯 (8)。化合物1~3、5和6对K562细胞增殖有抑制活性,其中1、2、5的作用较强,IC50分别为31 μg&;#8226;mL-1、29 μg&;#8226;mL-1、23 μg&;#8226;mL-1;3和6次之,在100 μg&;#8226;mL-1浓度下增殖抑制率分别为26.1 %和31.3 %。化合物5和6在无细胞毒的浓度下对 ECV304 细胞的缺氧损伤有保护作用。结论 化合物1~8系首次从该植物分离得到,其中1~3、5和6为该植物生物活性成分的首例报道。     

库拉索芦荟多糖和木立芦荟多糖体内抗肿瘤作用

目的探讨高纯度库拉索芦荟多糖及木立芦荟多糖的抗肿瘤作用及其与5-FU的协同作用。方法常规复制S180荷瘤小鼠模型,造模后24h给药,以5-FU做为阳性对照,观察25mg/（kg.d）库拉索芦荟多糖、木立芦荟多糖、5-FU＋库拉索芦荟多糖、5-FU＋木立芦荟多糖的抗肿瘤作用,连续给药10d后,检测各组药物的抑瘤率及联合用药的协同指数。结果25mg/（kg.d）库拉索芦荟多糖和木立芦荟多糖的抑瘤率分别为45.48%、47.68%;5-FU＋库拉索芦荟多糖及5-FU＋木立芦荟多糖联合用药组的抑瘤率则分别为66.00%和62.65%,协同指数分别为0.91、0.85。结论两种芦荟多糖均可有效的抑制肿瘤生长,和5-FU相似。两种芦荟多糖与5-FU联合用药具有明显的协同作用。     

甘露消毒丹灌肠治疗小儿疱疹性咽峡炎临床观察

目的探讨甘露消毒丹加减灌肠在小儿疱疹性咽峡炎中的治疗效果。方法采用前瞻性研究方法,纳入本院住院及门诊诊断为小儿疱疹性咽峡炎的患儿共计200例,随机数字表法随机分成2组,其中对照组给予常规治疗,观察组在对照组的基础上采取甘露消毒丹加减灌肠,对2组的症状几分进行比较,并比较2组的有效率。结果观察组在发热、纳差、咽痛、咽峡部疱疹等指标与对照组比较,差异具有统计学意义(P0.05)。总有效率观察组(97.00%)明显高于对照组(82.00%),差异有统计学意义(P0.05)。结论小儿疱疹性咽峡炎采用甘露消毒丹加减灌肠取得了良好的疗效,可有效提高症状积分和总有效率,有良好的临床应用价值。     

前胡药材的质量状况考察

目的:考察前胡药材的质量状况。方法:收集不同产地和生长年限的5种前胡药材,根据2010年版《中国药典》中方法,鉴别其性状、显微特征,并测定水分、总灰分、酸不溶性灰分、浸出物、挥发油以及白花前胡甲素、白花前胡乙素的含量。结果:前胡的浸出物、挥发油以及白花前胡甲素、白花前胡乙素的含量与其性状特征、木化程度存在一定的关联性和规律性。结论:应进行更广泛的取样分析,为科学制订前胡药材质量标准,指导药材生产提供参考。     

给冰箱做个正确的清理和保养

冰箱不定时清理会有哪些危害 滋生细菌 冰箱使用一段时间后,内部的气味会变得非常难闻,不仅影响食品味道,甚至可能会滋生细菌。     

专科组护士术前访视在乳腺癌手术中的效果

目的探讨手术室乳腺专科组护士术前访视在乳腺癌手术中的应用效果。方法选取乳腺科60例乳腺癌手术患者随机分为观察组(n=30),对照组(n=30)。观察组采用专科组护士进行术前访视,对照组采用一般的访视方法,登记两组术前访视单,记录SAS值、收集手术患者术后对手术室护士的满意度。结果观察组焦虑值低于对照组,心理状态和满意度明显高于对照组,p<0.05,有统计学意义。结论乳腺专科组护士对乳腺癌患者进行术前访视,能有效缓解患者焦虑,提高对护士的满意度。     

影响骨整合的因素分析

骨整合即在光镜水平下正常的改建骨和假体之间看不见软组织,假体与骨组织直接接触,其承受的负荷能通过这种直接接触持续不断地传递并分散到骨组织中.当今多数学者倾向于骨整合结合方式.纤维骨性结合是指假体与周围骨质之间不直接接触,而是有一层类似牙周膜的纤维膜相隔.本文主要通过文献资料法以及笔者多年工作经验,针对影响影响假体-骨界面骨整合的因素进行分析总结,希望能促进假体-骨界面骨整合技术的进一步发展.     

糖尿病周围神经病变的中医学认识

本文主要运用了文献资料法,针对糖尿病周围神经病变的中医学认识进行综述,希望能加深对糖尿病周围神经病变的认识.     

湖北恩施产厚朴叶形变化与药材质量的关系初探

目的:探索湖北恩施产厚朴叶形变化与药材质量的关系。方法:采用高效液相色谱法测定厚朴酚、和厚朴酚含量,流动相为甲醇-水(78∶22),检测波长为294 nm。结果:叶先端凸尖者、叶片短小者,厚朴叶的酚含量通常较高;在一定年限之内,树皮较厚者,厚朴皮的酚含量较高。结论:湖北恩施产厚朴叶形变化与叶自身酚含量的高低直接相关,与药材质量关联性不强。     

脱细胞基质材料制备方法及在骨关节软骨损伤修复中的应用

BACKGROUND: It has been reported that tissue-engineered acellular matrix can induce and promote epithelial cells and smooth muscle cells to evolve into a part of body in vivo compared with the normal extracellular matrix. OBJECTIVE: To investigate the effect of tissue-engineered acellular matrix in articular cartilage repair. METHODS: Totally 30 New Zealand rabbits were randomly allotted to fibroid tissue and acellular matrix groups (n=15 per group), and then articular cartilage defect models, 4 mm in diameter, were established at the white rabbit femoral condyle. Acellular cartilage matrix scaffold was prepared using bovine knee cartilage, and model rats in the acellular matrix group were repaired with acellular cartilage matrix scaffold and the others in the fibroid tissue group repaired with fibroid tissues. Finally, repair effects between two groups were compared. RESULTS AND CONCLUSION: The dark blue and porous tissue-engineered acellular matrix material could be found, with a diameter of 5 mm and moderate hardness, and exhibited certain flexibility after cross-linking. Hematoxylin-eosin staining showed that cell debris, blue-stained nuclear materials and residual extracellular matrix disappeared. Toluidine blue staining found that the porosity of the blue scaffold was 90%, and the swelling ratio was (1 314±337)%. The absorbance value in the acellular matrix group was significantly higher than that in the fibroid tissue group at 1, 3, 5, 7 and 9 days (P < 0.05). In the fibroid tissue group, defects filled with newborn fibrous scars were overt. By contrast, in the acellular matrix group, the white tissues covered the defect region with smooth surface, and the wound was basically healed, with an unclear boundary after 12 weeks. Moreover, blue-stained, small flattened cells appeared, subchondral bone structure was connected well with the cartilage, and the scaffold was directly degraded. In conclusion, the tissue-engineered acellular matrix material exhibits good biocompatibility, cell adsorption and hydrophilicity, and can promote the defect repair after articular cartilage injury. Therefore, it is a suitable substitute for articular cartilage repair.