一测多评法同时测定艾纳香油中5种成分

目的建立一测多评法同时测定艾纳香Blumea balsamifera(L.)DC.油中β⁃蒎烯、β⁃石竹烯、樟脑、α⁃石竹烯和龙脑5种成分的含有量。方法艾纳香油乙酸乙酯提取物的分析采用PEG⁃20 M柱(30 m×0.32 mm,1.0μm);程序升温;载气为高纯氮气(99.999%);FID检测器温度240℃,进样口温度240℃。以龙脑为内标,计算其他4种成分的相对校正因子,再测定其含有量。结果蒎烯、β⁃石竹烯、樟脑、α⁃石竹烯和龙脑分别在1.49~59.5μg/mL(r=0.9996)、2.22~88.8μg/mL(r=0.9996)、6.48~259μg/mL(r=0.9997)、3.64~146μg/mL(r=0.9991)和16.4~656μg/mL(r=0.9998)范围内线性关系良好,平均加样回收率(RSD)分别为97.4%(0.9%)、99.0%(1.3%)、98.9%(0.9%)、97.6%(0.9%)和99.7%(1.0%)。一测多评法所得结果接近于外标法。结论该方法准确稳定,重复性好,可用于艾纳香油的质量控制。     

清热化瘀汤对急性脑出血患者血清hs-CRP、血浆S100β蛋白和NSE的影响

目的:探究清热化瘀汤联合依达拉奉对急性脑出血患者血清超敏C反应蛋白(hs-CRP)、血浆S100β蛋白和神经元特异性烯醇化酶(NSE)的影响。方法:选择2017年5月—2019年5月在我院神经内科就诊符合纳入标准的80例急性脑出血患者,随机分为联合组(40例)和依达拉奉组(40例),两组均给予基础治疗和依达拉奉静脉滴注治疗,联合组则在此基础上加用清热化瘀汤。观察并比较两组的临床疗效、神经功能缺损程度评分(NIHSS)、格拉斯哥昏迷评分(GCS)、hs-CRP、S100β蛋白和NSE水平及脑血肿量。结果:联合组的总有效率为95%(38/40),显著高于依达拉奉组的总有效率75%(30/40)(P<0.05)。治疗后,两组NIHSS评分降低,GCS评分升高,且联合组NIHSS评分明显低于依达拉奉组(P<0.05),GCS评分高于依达拉奉组(P<0.05)。治疗后,两组hs-CRP、S100β蛋白和NSE水平及均脑血肿量均低于治疗前,且联合组hs-CRP、S100β蛋白和NSE水平及脑血肿量明显低于依达拉奉组(P<0.05)。结论:清热化瘀汤联合依达拉奉对急性脑出血患者具有良好的疗效,可显著改善其神经缺损,降低hs-CRP、S100β蛋白和NSE水平及脑血肿量。     

两种补肾方药对骨质疏松症患者骨密度及疼痛评分影响的Meta分析

目的:系统评价并对比以金匮肾气丸为主的补肾方药与以六味地黄丸为主的补肾方药在治疗骨质疏松症的临床疗效。方法:计算机检索SCI、PubMed、中国期刊全文数据库(CNKI)、Cochrane图书馆、EMbase、万方等各类中、英文数据库,检索年限截止至2019年3月,检索有关补肾方药治疗骨质疏松症的随机对照文献(Randomized Controlled Trial,RCTs)。采用RevMan 5.3软件进行数据的Meta分析。结果:纳入5项RCT分为两组(一组2项,一组3项),涉及受试者421例,Meta分析结果显示,在骨密度(Bone Mineral Density,BMD)方面:以金匮肾气丸为主的补肾方药,治疗组优于对照组最为明显(MD=-2.13,95%CI为-3.00^-1.26,P<0.00001);在视觉模拟疼痛评分(Visual Analogue Scale,VAS)方面:以金匮肾气丸为主的补肾方药,治疗组优于对照组最为明显(MD=0.03,95%CI为-0.01~0.06,P=0.10)。结论:以金匮肾气丸为主的补肾方药在治疗骨质疏松症的临床疗效比以六味地黄丸为主的补肾方药积极意义更高。     

Expression of Salivary S100A7 Levels in Stage I Oral Submucous Fibrosis: A Clinical and Laboratory Study

Background: Oral submucous fibrosis (OSF) is a chronic debilitating condition characterized by juxta-epithelial fibrosis. The main etiological agent associated with the high-risk precancerous condition is areca nut use. S100A7 is a member of the largest calcium-binding proteins exclusively found in vertebrates and are associated with the regulation of numerous intracellular and extracellular functions. The aim of this study was to investigate the expression of protein S100A7 in salivary samples of individuals with stage I OSF and healthy controls. Methods: This study included 63 participants, 30 of whom had OSF stage I and 33 healthy controls. Nonprobability quota sampling technique was utilized for recruitment of the study participants. A structured baseline questionnaire was used to collect demographic data. Saliva samples were collected by passive droll technique in a sterile container. Salivary levels of S100A7 were quantified by enzyme-linked immunosorbent assay. For the normality of the data Shapiro Wilk test was performed. Student t-test was commuted to evaluate the expression of S100A7 protein expression between both the study groups. Results: The mean salivary S100A7 value for stage I OSF group was 0.334 ng/ml, compared to 0.172 ng/ml for healthy controls. Student t-test reported a statistically significant difference, indicating higher levels of S100A7 in stage I OSF group than in healthy controls (p < 0.001). In the individual group analysis, a significant negative correlation was found between salivary S100A7 and duration of areca nut use (r = –0.45, p = 0.009) and gutka chewing (r = –0.20, p = 0.03), while a significant positive correlation was found between salivary S100A7 and mouth opening (r = 0.03, p = 0.04). Conclusions: Higher levels of S100A7 protein level was seen in stage I OSF group in comparison to the healthy individuals. Results of our study suggest that S100A7 could be used as a surrogate assessment to identify patients at risk of OSF development.     

维生素A对糖基化终末产物诱导的角膜上皮细胞损伤的作用及机制研究

目的糖基化终末产物(AGEs)在糖尿病患者在角膜外伤或接受角膜手术时极易出现角膜上皮愈合延迟甚至不愈的过程中起着关键作用,本文研究维生素A(VA)对以糖基化终末产物AGE-BSA诱导角膜上皮细胞损伤的影响及可能的作用机制。方法以HCE-2细胞为研究对象,AGE-BSA为诱导剂,试剂盒检测VA对细胞凋亡率及线粒体膜电位的影响,荧光显微镜检测细胞内凋亡小体,Western blot检测相关的蛋白表达量。结果VA抑制AGE-BSA诱导的HCE细胞凋亡,调节AGEBSA引起的凋亡相关蛋白的变化。同时,VA与JNK的抑制剂同等程度的减少AGE-BSA诱导的JNK和NF-κB的磷酸化。结论VA可能通过抑制JNK介导的NF-κB炎症通路而起到减少AGE-BSA诱导的角膜上皮细胞凋亡的作用,可以作为糖尿病患者角膜上皮损伤的保护剂。     

益气养阴解毒方联合环磷腺苷葡胺注射液治疗病毒性心肌炎35例

目的:探讨益气养阴解毒方联合环磷腺苷葡胺注射液对病毒性心肌炎患者心功能及外周血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、转化生长因子-β1(transforming growth factor-β1,TGF-β1)和干扰素-γ(interferon-γ,IFN-γ)水平的影响。方法:将70例病毒性心肌炎患者按照随机数字法分为对照组和观察组,每组各35例。两组患者均给予常规治疗,对照组在常规治疗的基础上给予环磷腺苷葡胺注射液静脉滴注,观察组在对照组的基础上联合益气养阴解毒方治疗。比较两组患者的临床疗效及治疗前后左室短轴缩短分数(left ventricular shortening fraction,LVFS)、左心室射血分数(left ventricular ejection fraction,LVEF)、心脏指数(cardiac index,CI)、TNF-α、TGF-β1和IFN-γ水平。结果:观察组有效率为88.57%,对照组有效率为68.57%,两组患者有效率比较,差异有统计学意义(P<0.05)。两组患者治疗后LVEF、LVFS高于本组治疗前,且观察组高于对照组,差异具有统计学意义(P<0.05)。两组患者治疗后TGF-β1、TNF-α水平低于治疗前比较,IFN-γ高于治疗前,且观察组TGF-β1、TNF-α低于对照组,IFN-γ高于对照组,差异均有统计学意义(P<0.05)。两组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论:益气养阴解毒方联合环磷腺苷葡胺注射液治疗病毒性心肌炎疗效更显著,可减轻机体的炎症反应,恢复患者心功能。     

A Probabilistic Classification Tool for Genetic Subtypes of Diffuse Large B Cell Lymphoma with Therapeutic Implications

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三七片DNA条形码分子鉴定及方法学考察

目的为研究DNA条形码技术在中成药鉴定中的应用,以三七片为研究对象,对方法的适用性、专属性与精密度进行考察。方法收集15批次市售三七片样品,考察三七片DNA提取条件,并对"中药材DNA条形码分子鉴定法指导原则"中PCR扩增、序列获得、结果判定等方法适用性进行确认;收集三七、人参、西洋参,制作三七片及其掺伪品,考察方法的专属性和重现性。结果三七片取样量100 mg,56℃水浴8 h所获得DNA的质量浓度平均值为60.7 ng/μL,PCR扩增、序列获得和结果判定均可获成功;三七、人参和西洋参的ITS2序列长度均为230bp,三七与人参、三七与西洋参的序列间均存在7个稳定的SNP位点,自制三七片和掺伪三七片均可成功获得ITS2序列,不同比例三七与人参、三七与西洋参的测序峰图在SNP位点处呈现相应峰高比的SNP套峰具备专属性;重复性、中间精密度和重现性考察符合《中国药典》2015年版(通则9101)相关要求。结论 ITS2序列作为DNA条形码能够稳定、准确鉴定三七片的原料药材,具备良好的专属性和精密度,三七片DNA条形码分子鉴定法将为保障三七片临床用药安全提供新的技术手段,并对《中国药典》其他收载单方制剂的鉴定提供参考。