新疆和田地区居民干眼现况调查与分析

目的:评估中国新疆和田地区居民干眼的患病率和潜在的相关危险因素。方法:2019-01/09在中国新疆和田地区采取立意抽样的方法选取105个村18～98岁居民共计6 027人作为受试者,并进行横断面研究。调查者采用眼表疾病指数(OSDI)问卷的方式收集干眼主观症状,检查荧光素染色泪膜破裂时间(FBUT)及泪液分泌试验(SchirmerⅠ试验)来收集客观体征,从而分析干眼患病率及其危险因素。结果:从中国新疆和田地区居民中招募18～98岁共6 339名受试者进行相关眼部检查和问卷调查,其中有效问卷6 027份,有效回收率为95.08%。6 027名受试者中干眼检出率为40.37%(2 433/6 027),其中男性、女性干眼检出率分别为36.47%(846/2 320)、42.81%(1 587/3 707);18～24岁、25～34岁、35～44岁、45～54岁、55～64岁、≥65岁干眼检出率分别为13.77%、15.67%、33.31%、46.35%、47.65%、53.50%。根据干眼严重程度分为轻度干眼、中度干眼及重度干眼,它们的构成比依次为80.11%、19.03%、0.86%。...     

维吾尔族医学常用药材“夏合坦然”考证及鉴定

目的:明确维吾尔族医学常用药材夏合坦然基原,纠正使用药材品种与考证品种基原不符的问题。方法:采用经典形态鉴别、显微鉴别、同步辐射鉴定、分子鉴定方法进行鉴定。结果:新疆各地的维吾尔医医院中应用的夏合坦然药材植物形态鉴别、显微鉴别、同步辐射鉴定及分子鉴定结果与正确基原不符。结论:误用品以大戟科大戟属植物地锦草居多,而正品应为罂粟科烟堇属植物烟堇。     

骆驼刺中总还原糖与芦丁的含量测定

目的：建立测定骆驼刺中总还原糖与芦丁含量的方法,并对不同产地不同月份的样品含量进行比较。方法：采用紫外分光光度法测定骆驼刺中总还原糖的含量,以葡萄糖为对照品,检测波长为620nm;采用高效液相色谱法测定芦丁的含量,色谱柱为SymmetryShieldRp-18（150mm×3.9mm,5μm）,流动相为乙腈-0.1%磷酸水溶液（19∶81,V/V）,检测波长为360nm,柱温为30℃。结果：葡萄糖检测浓度的线性范围为3.85～70.33μg·mL-（1r=0.9959）,平均加样回收率为99.6%,RSD=1.5%（n=6）;芦丁检测浓度的线性范围为4.95～59.40μg·mL-（1r=0.9996）,平均加样回收率为99.3%,RSD=2.08%（n=6）。不同产地不同月份的骆驼刺中芦丁和总还原糖含量有一定差异,其中新疆乌鲁木齐和且末地区在7-8月份采收的骆驼刺中总还原糖和芦丁的含量较高。结论：本试验结果可为骆驼刺的种植和采收的研究奠定基础。     

芸香药材中花椒毒素的HPLC测定方法研究

目的:筛选出在芸香中提取花椒毒素的方法,建立HPLC法测定花椒毒素的含量.方法:在避光条件下,利用花椒毒素在二氯甲烷和乙酸溶液中均有较好溶解度,通过二氯甲烷提取,挥干,残渣加5％乙酸溶液溶解,过滤,进样.采用C18色谱柱,流动相为乙腈:0.02 mol·L-1乙酸铵(45:55),流速1.0 mL·min-1,检测波长...     

Comparison of the efficacy and safety of the adalimumab biosimilar TQ-Z2301 and adalimumab for the treatment of Chinese patients with active ankylosing spondylitis: a multi-center,randomized, double-blind,phase III clinical trial

Clinical Rheumatology - To assess the clinical equivalence of TQ-Z2301, a biosimilar of adalimumab, to the reference adalimumab in the treatment of Chinese patients with active ankylosing...     

反复脑缺血再灌注小鼠造模影响因素的Logistic回归分析

目的分析反复脑缺血再灌注（RCIR）小鼠造模过程中体质量、操作者因素与造模结局之间的关系,以期降低造模过程中小鼠死亡率。方法取健康SPF级雄性昆明小鼠47只,进行编号,并记录体质量,进行RCIR造模,在造模的过程中始终标明小鼠编号进行跟踪,直至该小鼠成活或死亡时获取造模结局及操作者的数据,最后对记录的数据按照造模结局进行分类,将体质量、操作者作为变量,进行统计学分析,并综合进行Logistic回归多元统计分析。结果在体质量32.5~37.7 g范围内小鼠体质量与造模结局无关（P〉0.05）,操作者与造模结局也无关（P〉0.05）。结论在一定体质量范围内,造模结局与小鼠体质量、年资相仿并经过同期培训的手术操作者无关,对造模结局没有影响的小鼠体质量差可以扩大到5.2 g。     

Therapeutic effects of rosmarinic acid on airway responses in a murine model of asthma

Rosmarinic acid (RA) is an active component of a traditional Chinese herbal medicine. Previously, we reported that RA exerted a strong anti-inflammatory effect in a mouse acute lung injury model. Therefore, we hypothesized that RA might also have potential therapeutic effects in a murine model of asthma. In this study, we aimed to evaluate the anti-asthmatic activity of RA and explored its possible molecular mechanisms of action. Female BALB/c mice that had been sensitized to and challenged with ovalbumin (Ova) were treated with RA (20 mg/kg) 1 h after challenge. The results showed that RA greatly diminished the number of inflammatory cells and the production of Th2 cytokines in the bronchoalveolar lavage fluid (BALF); significantly reduced the secretion of total IgE, Ova-specific IgE, and eotaxin; and markedly ameliorated airway hyperresponsiveness (AHR) compared with Ova-induced mice. Histological studies further revealed that RA substantially decreased inflammatory cells infiltration and mucus hypersecretion compared with Ova-induced mice. Moreover, our results suggested that the protective effects of RA were mediated by the inhibition of JNK and p38 MAPK phosphorylation and nuclear factor-κB (NF-κB) activation. Furthermore, RA treatment resulted in a significant reduction in the mRNA expression of AMCase, CCL11, CCR3, Ym2 and E-selectin in lung tissue. These findings suggest that RA may effectively delay the development of airway inflammation and could thus be used as a therapy for allergic asthma.     

Recombinant Newcastle disease virus expressing H9 HA protects chickens against heterologous avian influenza H9N2 virus challenge

In order to produce an efficient poultry H9 avian influenza vaccine that provides cross-protection against multiple H9 lineages, two Newcastle disease virus (NDV) LaSota vaccine strain recombinant viruses were generated using reverse genetics. The recombinant NDV-H9Con virus expresses a consensus-H9 hemagglutinin (HA) that is designed based on available H9N2 sequences from Chinese and Middle Eastern isolates. The recombinant NDV-H9Chi virus expresses a chimeric-H9 HA in which the H9 ectodomain of A/Guinea Fowl/Hong Kong/WF10/99 was fused with the cytoplasmic and transmembrane domain of the fusion protein (F) of NDV. Both recombinant viruses expressed the inserted HA stably and grew to high titers. An efficacy study in chickens showed that both recombinant viruses were able to provide protection against challenge with a heterologous H9N2 virus. In contrast to the NDV-H9Chi virus, the NDV-H9Con virus induced a higher hemagglutination inhibition titer against both NDV and H9 viruses in immunized birds, and efficiently inhibited virus shedding through the respiratory route. Moreover, sera collected from birds immunized with either NDV-H9Con or NDV-H9Chi were able to cross-neutralize two different lineages of H9N2 viruses, indicating that NDV-H9Con and NDV-H9Chi are promising vaccine candidates that could provide cross-protection among different H9N2 lineage viruses.     

Neutralizing antibodies to gB based CMV vaccine requires full length antigen but reduced virus neutralization on non-fibroblast cells limits vaccine efficacy in the guinea pig model

Cytomegalovirus is a leading cause of congenital disease and a vaccine is a high priority. The viral gB glycoprotein is essential for infection on all cell types. The guinea pig is the only small animal model for congenital CMV (cCMV), but requires guinea pig cytomegalovirus (GPCMV). Various GPCMV gB vaccine strategies have been investigated but not with a full length protein. Previous GPCMV gB vaccines have failed to fully protect against cCMV, with approximately 50% efficacy. In an effort to define the basis of GPCMV gB based vaccine failure, we evaluated recombinant defective Ad vectors encoding GPCMV gB full length (gBwt), or truncated protein lacking transmembrane domain (gBTMD). Both candidate vaccines evoked high anti-gB titers and neutralized virus infection on fibroblast cells but had varying weaker results on non-fibroblasts (renal epithelial and placental trophoblasts). Non-fibroblast cells are dependent upon the viral pentamer complex (PC) for endocytic pathway cell entry. In contrast, fibroblasts cells that express the viral receptor platelet derived growth factor receptor alpha (PDGFRA) to enable entry by direct cell fusion independent of the PC. Anti-gBwt sera was approximately 2-fold (renal epithelial) to 3-fold (fibroblasts) more effective at neutralizing virus compared to anti-gBTMD sera. Both gB vaccines were weakest against virus neutralization on trophoblasts. Knockout of PDGFRA cell receptor on fibroblast cells (GPKO) rendered virus dependent upon the PC pathway for cell entry and anti-gB GPCMV NA₅₀ was more similar to epithelial cells. In a gBwt vaccine protection study, vaccination of animals significantly reduced, but did not prevent dissemination of wild type GPCMV challenge virus to target organs. Depletion of complement in vivo had limited impact on vaccine efficacy. Overall, a full length gB antigen has the potential to improve neutralizing antibody titer but fails to fully prevent virus dissemination and likely congenital infection.