Association of job stress,CLOCK gene polymorphism and their interaction with poor sleep quality

Job stress and the Circadian Locomotor Output Cycles Kaput (CLOCK) gene could affect circadian rhythm and sleep quality. The main aim of our present study was to investigate the association of job stress, CLOCK gene polymorphism and their interaction with sleep quality in a non‐clinical Chinese Han population, which has not been reported to date. Using a cross‐sectional design, 450 subjects were recruited in Beijing. Sleep quality was measured with the Pittsburgh Sleep Quality Index (PSQI) and job stress was measured with the Work Stress Scale. CLOCK gene rs11932595 polymorphism was genotyped in 297 blood samples. Correlation analysis showed a close but different association of high job stress with the PSQI and its components. Analysis of variance showed significant main effects of the CLOCK gene rs11932595 polymorphism. G‐allele carriers had a higher score in the PSQI, sleep duration, sleep latency and sleep disturbances. Further interaction analyses showed an ordinal interaction on sleep duration, and a disordinal interaction on daytime dysfunction. Specifically, G‐allele carriers had poorer sleep duration than AA homozygotes when in high job stress, while the two subgroups displayed similar sleep duration when in low job stress, conforming to the diathesis–stress model. In comparison to G‐allele carriers, AA homozygotes experienced less daytime dysfunction when in low job stress whereas more daytime dysfunction when in high job stress, fitting with the differential susceptibility model. As genetic links have been revealed, our investigation might be conducive for elucidating aetiological factors for sleep quality and targets for implementing interventions to attain good sleep quality.     

国医大师刘柏龄治疗脊髓型颈椎病用药规律探析＊

目的 挖掘、分析国医大师刘柏龄治疗脊髓型颈椎病的用药特点及组方规律。方法 收集120份符合纳入标准的刘柏龄教授治疗脊髓型颈椎病的验案，利用Epidata 3.0软件建立方药数据库，使用IBM SPSS Modeler 18.2软件中基于关联规则的Apriori算法，对处方中的中药及其分类的使用频次、中药之间的应用关系进行分析。结果 纳入处方共涉及中药104味，其中使用频次较高的中药依次为黄芪、当归、附子、丹参、赤芍等，用药以补虚药、活血化瘀药、祛风湿药、平肝息风药为主，辅以解表、温里、化湿等药。在关联规则集中，在支持度20%和置信度90%的情况下分析得到二阶、三阶和四阶关联规则，核心药物群包括黄芪、当归、赤芍、附子、红花、桃仁、鸡血藤、地龙、川芎等。结论 刘柏龄治疗脊髓型颈椎病用药具有补虚祛邪并重的鲜明特点，主要采取益气温里助阳、活血化瘀通络、补肾强骨、祛除风湿之邪的治疗原则。     

CD2AP inhibits metastasis in gastric cancer by promoting cellular adhesion and cytoskeleton assembly

Diffuse gastric cancer (DGC) is a lethal malignancy lacking effective systemic therapy. Among the most provocative recent results in DGC has been that the alter of the cellular cytoskeleton and intercellular adhesion. CD2-associated protein (CD2AP) is one of the critical proteins regulating cytoskeleton assembly and intercellular adhesion. However, no study has investigated the expression and biological significance of CD2AP in gastric cancer (GC) to date. Therefore, the aim of our study was to explore if the expression of CD2AP is associated with any clinical features of GC and to elucidate the underlying mechanism. Immunohistochemistry of 620 patient tissue samples indicated that the expression of CD2AP is downregulated in DGC. Moreover, a low CD2AP level was indicative of poor patient prognosis. In vitro, forced expression of CD2AP caused a significant decrease in the migration and invasion of GC cells, whereas depletion of CD2AP had the opposite effect. Immunofluorescence analysis indicated that CD2AP promoted cellular adhesion and influenced cell cytoskeleton assembly via interaction with the F-actin capping protein CAPZA1. Overall, the upregulation of CD2AP could attenuate GC metastasis, suggesting CD2AP as a novel biomarker for the prognosis and treatment of patients with GC.     

Optical coherence tomography angiography for marginal corneal vascular remodelling after pterygium surgery with limbal-conjunctival autograft

PurposeTo demonstrate the marginal corneal vascular remodelling using optical coherence tomography angiography (OCTA) after pterygium surgery.MethodsTwenty-two eyes of 19 patients (8 males, 11 females; age, 58.68 ± 0.34 years) with primary grade-T3 nasal pterygium were enroled in this study. The eyes underwent excision of the pterygium followed by a free limbal-conjunctival autograft. OCTA was performed in the nasal limbal area before surgery and at 10 days, 1 month, and 3 months after surgery. The scans were analyzed in terms of postoperative vascular remodelling of the autograft and marginal corneal vascular arcades (MCAs).ResultsPreoperatively, the pterygium presented as abnormal centripetal vascular growth in OCTA scans. The conjunctival vessel density in the nasal quadrant was 29.26% ± 1.00%, 15.80% ± 0.83%, 19.80% ± 0.88%, and 20.26% ± 0.89% before and 10 days, 1 month, and 3 months, respectively, after surgery (F = 1.55, P < 0.01). The vessel density of MCAs was 28.33% ± 0.88%, 42.09% ± 0.41%, and 42.46% ± 0.31% 10 days, 1 month, and 3 months, respectively, after surgery (F = 188.2, P < 0.01).ConclusionsWe describe a new application of OCTA for MCA vasculature imaging. Vascular remodelling of the graft and MCAs appeared at 1 month and continued for 3 months after surgery.Subject terms: Conjunctival diseases, Corneal diseases     

结核多肽壳聚糖纳米粒的制备及表征

采用离子交联法制备壳聚糖纳米粒(CS-NPs)载体,并以粒径和多分散系数(PDI)为主要评价指标优化了工艺参数。通过文献检索,筛选出结核分枝杆菌复合群(Mycobacterium tuberculosis complex,MTC)标准强菌株H37Rv的保护性抗原蛋白Rv0180c(GLDKNKFDIRVVSPDEARRLY),经异硫氰荧光素(FITC)标记后载入优化的空白CS-NPs中,获得结核多肽壳聚糖纳米粒(Pep-CS-NPs)。所得空白CS-NPs的平均粒径、PDI和ζ电位分别为(148.13±2.24)nm、0.197±0.013和(+18.00±0.89)mV;4℃放置28 d,粒径未见显著增大。Pep-CS-NPs的平均粒径、PDI和ζ电位分别为(186.93±8.80)nm、0.254±0.014和(+12.07±1.68)mV,包封率和载药量为(45.20±2.95)%和(12.92±1.12)%。体外释放结果表明,Pep-CS-NPs缓慢释放多肽,48 h累积释放率为56.6%。本试验表明,离子交联法制备载多肽CS-NPs的工艺简便稳定、条件温和,所得制品粒径大小适宜、分布均匀,且具有明显缓释作用,有望作为治疗结核病的新型疫苗。     

新型国产商业化链球菌药敏板的体外药敏检测性能评估

目的 通过考察国产商业化链球菌药敏板与微量肉汤稀释法对327株链球菌进行基本一致性(E A)和分类一致性(C A)的测定结果,评估该药敏板的检测性能.方法 收集2018-2019年来源于郑州市中心医院、郑州市人民医院、南阳市中心医院和河南省人民医院的327株链球菌属不同种类的临床菌株,采用国产商业化链球菌药敏板与微量肉汤稀释法同时测定药敏试验结果的EA和CA等.结果 新型国产商业化链球菌药敏板的EA为99.53％,其中头孢曲松等16种抗菌药物的EA均为100.00％;氨苄西林、美罗培南、复方磺胺甲噁唑的EA分别为97.86％、96.94％、97.55％;红霉素/克林霉素诱导试验的EA为98.17％;在CA方面,两种方法的C A为97.40％,非常重大误差为0.09％,重大误差为0.59％,微小误差为1.92％.结论 新型国产商业化链球菌药敏板与微量肉汤稀释法比较,E A和C A均大于94.00％,非常重大误差小于1.50％,重大误差小于3.00％,体外药敏试验检测性能良好.     

CT测量成人寰枢椎后方部分结构数据设计寰枢椎板间融合器

文题释义： 寰枢椎脱位：可分为可复性、难复性和不可复性脱位3种类型，其可导致C_1/2水平脊髓压迫而造成神经功能障碍症状，临床上多需要进行手术治疗，后路钉棒固定融合术是最常用的手术方式。 寰枢椎板间融合器：与寰枢椎侧块关节融合器不同，其直接固定在寰椎后弓与枢椎椎板骨面上，是一种新型融合器，此融合器可配合广泛使用的后路钉棒内固定手术，置入简便，增加植骨与寰椎后弓及枢椎椎板骨面的接触应力，促进骨性融合。 背景：寰枢椎融合器一直以侧块关节融合器为研究热点，但其置入操作风险过大，目前临床上一直缺乏可广泛应用的寰枢椎融合器。 目的：探究成人后路寰枢椎板间融合器的CT影像解剖学可行性并给出初步设计。 方法：对100例成人(男50例，女50例)寰枢椎CT三维成像进行测量，测量指标包括寰椎单侧后弓长度、寰椎后弓厚度、枢椎单侧椎板长度、枢椎椎板厚度、寰椎后弓上缘至枢椎椎板下缘距离，分析寰枢椎后方部分结构的CT测量数据并设计寰枢椎板间融合器。 结果与结论：①男、女性的寰椎单侧后弓长度分别为(23.41±1.48) mm和(22.23±1.25) mm，寰椎后弓厚度分别为(6.00±0.96) mm和(5.28±0.78) mm，枢椎单侧椎板长度分别为(18.54±2.23 )mm和(17.31±0.91) mm，枢椎椎板厚度分别为(5.12±1.31) mm和(4.98±1.26) mm，寰椎后弓上缘至枢椎椎板下缘距离分别为(32.63±2.76) mm和(31.39±4.04) mm；②不同性别的双侧测量数据差异无显著性意义(P > 0.05)；男性的寰椎单侧后弓长度、寰椎后弓厚度、枢椎单侧椎板长度大于女性，差异有显著性意义(P < 0.05)；男性与女性的枢椎椎板厚度、椎后弓上缘至枢椎椎板下缘距离差异无显著性意义(P > 0.05)；③寰枢椎后方部分结构的CT测量结果提示寰枢椎板间融合器是可行的；④寰枢椎板间融合器初步设计成功，并获得国家专利；⑤提示寰枢椎板间融合器具有应用可行性，可用于寰枢椎后路钉棒固定融合过程中，提升寰枢椎间的植骨融合率。 ORCID: 0000-0002-2101-1900(邹小宝)中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程     

中西医结合治疗对单纯疱疹病毒性角膜炎患者视力恢复及血清微量元素的影响

目的:探讨中西医结合治疗对单纯疱疹病毒性角膜炎(HSK)患者视力恢复及血清微量元素的影响。方法:选取2015年1月至2016年10月内蒙古包钢医院收治的HSK患者86例作为研究对象,按照随机数字表法随机分为对照组和观察组,每组43例。对照组采用更昔洛韦滴眼液及硫酸软骨素滴眼液治疗,观察组在对照组基础上结合清肝明目汤进行治疗,2组均连续治疗4周。比较2组患者临床疗效;比较治疗前后2组患者视力恢复状况、血清微量元素及泪液免疫因子水平。结果:治疗后,观察组治疗有效率显著高于对照组,差异有统计学意义(P0.05);治疗后观察组患者视觉模拟评分及视力均显著高于治疗前与对照组,差异有统计学意义(P0.05);治疗后观察组铁离子、钙离子及铜离子水平低于治疗前与对照组,差异有统计学意义(P0.05);血清锌离子水平明显高于治疗前与对照组(P0.05),差异有统计学意义(P0.05);治疗后观察组患者泪液IgA、IgG及C3水平明显高于治疗前与对照组,差异有统计学意义(P0.05)。结论:中西医结合治疗HSK有效促进患者视力的恢复,调节血清微量元素的同时增强患者泪液免疫因子水平,疗效显著优于单用西药治疗。     

行为决策的评估理论构建研究

本研究构建了行为决策的评估理论,揭示人类行为发生、发展和改变的规律,为开展健康相关行为干预提供科学依据。本理论认为,所有的人类行为都是个体为了生存和发展,在对内、外部刺激进行评估后所做出的主动反应。个体以基因遗传、自我图式、群体规范和直觉为参照系,对内、外部刺激的重要性、安全性威胁和获益性,以及个体所拥有的行为资源进行感觉性评估和社会性评估,用以做出生理性反应和社会性行为决策,并通过感受器、中枢神经和效应器,形成“刺激-评估-行为决策-行为”循环。开展行为干预时,应通过与个体进行讨论和分析,帮助其发现和确认刺激的重要性、安全性、获益性及其拥有的行为资源。