十二指肠钩虫不同蚴龄感染小白鼠的实验研究

用蚴龄20d、40d 和60d 的幼虫,分别感染了3～4个月的小白鼠,每鼠经左腹部感染幼虫300条。在感染24h,3组幼虫均移行至肺脏,但在70d 后肺部仍有幼虫;自感染9d 后蚴龄20d 先移行至小肠,于11d 后蚴龄40d 和蚴龄60d 亦达小肠;感染24h 至70d 各部位组织均可检出未发育的Ⅲ期幼虫。     

19例SARS死亡病例临床分析

为分析严重急性呼吸综合征(SARS)死亡患者的临床特征、治疗及死亡相关因素,对2003年5月6日至6月27日收治的220例SARS患者中19例死亡患者的临床、实验室、影像学资料、临床治疗及死亡相关因素进行回顾性分析。结果19例死亡SARS患者年龄24～86岁,平均(57.63±16.76)岁。大于45岁13例(68.4%)。男性15例(78.9%),女性4例(21.1%)。有明确接触史19例(100%)。并存基础病13例(68.4%)。死亡距发病时间14～54d,平均(35.37±11.66)d。临床表现主要有发热19例(100%),咳嗽12例(63.2%),畏寒5例(26.3%),气促10例(52.6%),胸闷11例(57.9%),肌痛5例(26.3%),腹泻6例(31.6%)。实验室检查血常规中早期白细胞总数正常或降低,淋巴细胞降低。血气分析低氧血症16例(84.2%);心肌酶肌酸激酶心肌型同工酶(CKMB)、乳酸脱氢酶(LDH)、α羟丁酸脱氢酶(HBDH)随病情的严重程度而进行性升高。T细胞亚群CD3、CD4、CD8绝对值随病情的严重程度而进行性降低。胸部X线双侧、肺病变面积>1/3者19例(100%)。临床诊断继发混合感染(细菌、真菌)19例(100%)。采用综合治疗,其中激素18例(94.7%)、机械通气15例(78.9%)。死于呼吸衰竭18例(94.7%)。提示年龄、合并基础病、肺部病变程度、低氧血症、T细胞亚群CD3、CD4绝对值降低、激素剂量、混合感染等是影响S     

O,O′-二烷基-O″-(5-取代-3-苯并噻吩乙腈肟)磷酸酯及硫代磷酸酯的合成

合成了18个O,O′-二烷基-O″-(5-取代-3-苯并噻吩乙腈肟)磷酸酯及硫代磷酸酯类化合物(Ⅰ_1～18)。初步杀螺试验结果表明,其中5个化合物,即Ⅰ_2,3,7,11,12有明显的杀螺增效作用。     

广东汉族人群HLA-Cw多态性及单体型分析

目的检测广东汉族人群HLA．Cw及HLA-A、B、DRB1基因频率，分析该人群HLA-Cw等位基因多态性及其单体型特点。方法骨髓移植供者185例，抗凝血提取DNA，半量全自动PCR-RSSO分型检测HLA-A、B、Cw、DRB1基因型。结果在分辨水平检出Cw等位基因11个，其中分布频率较高的依次为：Cw*03(0．2580)、Cw*07(0．1887)、Cw*01(0．1732)、Cw*08(0．1070)。统计分析呈现显著连锁不平衡的HLA-Cw．A单体型7个，HLA-Cw-B单体型20个，HLA—Cw．DRB1单体型10个。结论广东汉族群体HLA-Cw基因具有较为丰富的多态性，其与HLA-A、B、DRB1间连锁不平衡单体型具有地区性遗传特征。     

乙型肝炎病毒表面抗原单链抗体细胞内免疫抗乙型肝炎病毒基因治疗研究

目的 :研究乙型肝炎病毒表面抗原 (HBsAg)人源单链可变区抗体 (ScFv)细胞内免疫抗乙型肝炎病毒(HBV)基因治疗的作用。方法 :用噬菌体表面展示技术筛选特异性的HBsAg人源单链可变区抗体 ,聚合酶链反应(PCR)法扩增HBsAg单链抗体基因 ,并构建表达HBsAgScFv基因的重组逆转录病毒载体pLXSN HBsAgScFv,转染PA317细胞 ,将转染细胞分泌的假病毒颗粒感染 2 2 15细胞 ,酶联免疫吸附法 (ELISA)检测其上清HBsAg和HBeAg ,定量检测HBVDNA。结果 :成功筛选出HBsAgScFv ,PCR扩增出 75 0bp的全基因 ,构建HBsAgScFv基因的逆转录病毒载体 ,转染PA317细胞 ,在上清中检测出含HBsAgScFv假病毒颗粒的存在 ,上清感染 2 2 15细胞后第 3、5、7、14天 ,HBsAg、HBeAg逐渐下降 ,到第 14天时HBsAg已变为阴性 ,DNA定量检测无明显变化。结论 :HBsAgScFv能成功地在逆转录病毒载体中表达 ,并有抑制HBsAg、HBeAg表达的作用     

Potential contribution of nuclear factor-kappaB to cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits.

Nuclear factor-kappaB (NF-kappaB) plays a key role in inflammation, which is involved in the development of cerebral vasospasm after subarachnoid hemorrhage (SAH). In the present study, we assessed the potential role of NF-kappaB in regulation of cerebral vasospasm. Nuclear factor-kappaB DNA-binding activity was measured in cultured vascular smooth muscle cells (VSMCs) treated with hemolysate and pyrrolidine dithiocarbamate (PDTC, 80 micromol/L), an inhibitor of NF-kappaB. Forty-two rabbits were divided into three groups: control, SAH, and PDTC groups (n=14 for each group). The caliber of the basilar artery was evaluated. Nuclear factor-kappaB DNA-binding activity and the gene expression levels of cytokines and adhesion molecules in the basilar artery were measured. Immunohistochemical study was performed to assess the expression and localization of tumor necrosis factor (TNF)-alpha, intercellular adhesion molecule (ICAM)-1, and myeloperoxidase (MPO). It was observed that NF-kappaB DNA-binding activity was significantly increased by treatment with hemolysate in cultured VSCMs, but this increase was suppressed by pretreatment with PDTC. Severe vasospasm was observed in the SAH group, which was attenuated in the PDTC group. Subarachnoid hemorrhage could induce increases of NF-kappaB DNA-binding activity and the gene expression levels of TNF-alpha, interleukin (IL)-1 beta, ICAM-1, and vascular cell adhesion molecule (VCAM)-1, which were reduced in the PDTC group. Immunohistochemical study demonstrated that the expression levels of TNF-alpha, ICAM-1, and MPO were all increased in the SAH group, but these increases were attenuated in the PDTC group. Our results suggest that NF-kappaB is activated in the arterial wall after SAH, which potentially leads to vasospasm development through induction of inflammatory response.     

RELATIONSHIP BETWEEN THE CLINICAL USE OF ANTIBIOTIC AND PRODUCTION OF ESBLS

Extended -spectrum β -lactamases(ESBLs)aremainlyproducedbymembersofthefamilyEnter obacteriaceaewhichcanhydrolyzeβ -lactamantibi oticsincludingthethird - generationcephalosporinandaztreonam ,theESBLs- producingbacillishowedmedian -highresistancetoceftazidimeandaztreonamparticularly[1] .NowadaystheprevalenceofESBLs -producingstrainshavebeenreportedinmanyareasaroundtheworld[2 ] .ButthereisfewinformationabouttherelationshipbetweentheuseofantibioticandtheproductionofESBLs .Weperformedastud…     

神经精神性系统性红斑狼疮的磁共振表现

目的探讨神经精神性系统性红斑狼疮(NPSLE)的磁共振诊断价值。方法回顾性分析经临床证实的16例NPSLE的头颅磁共振表现。结果16例NPSLE患者的头颅MRI异常者13例,阳性率81.3%。其MRI表现为:(1)13例均为大脑半球受累,灰白质均可累及;(2)脑内多发片状异常信号8例(61.5%),分布无规律;(3)脑梗塞7例,脑水肿4例,出血2例;(4)脑萎缩2例;(5)5例增强扫描均无明显强化。结论结合临床资料,MRI可以诊断NPSLE。     

错畸形患者的Howes值测量分析

目的:本研究采用Howes法评估牙弓骨量与牙量的协调性,探讨其在正畸临床诊断与矫治设计确定拔牙与非拔牙中的应用价值。方法:选取第四军医大学口腔医学院正畸门诊患者80人,男、女拔牙与非拔牙各20例。测量牙量(TM)、双尖牙弓宽径(PMD)、双尖牙根尖基骨宽径(PMBAW)、基骨弓长度(BAL),并计算PMD/TM、PMBAW/TM、BAL/TM值,对其进行统计学处理与分析。结果:男女两组比较TM、PMD、PMBAW、BAL值有显著性差异,男性大于女性;但PMD/TM、PMBAW/TM、BAL/TM值无统计学意义;拔牙组与非拔牙组比较,PMD/TM、PMBAW/TM、BAL/TM值有显著性差异。结论:采用Howes法评估牙弓骨量与牙量对正畸临床的诊断和拔牙与非拔牙矫治设计有一定的指导意义。     

磁共振成像对胸膜外脂肪显示的研究

目的：研究MRI对正常及异常胸膜外脂肪显示的价值。材料和方法：对21例健康志愿者进行了MRI化学位移同相位成像、反相位成像、T1WI、T2WI及STIR序列扫描，并对胸膜外脂肪的绝对信号强度及信号强度比进行比较分析，同时还对20例累及胸膜外脂肪的病人进行检查。结果：在MRI各序列中，化学位移反相位成像显示胸膜外脂肪最佳，各序列绝对信号强度没有差别，而信号强度比存在差异。异常的胸膜外脂肪可以表现为移位、受压、中断、增厚及信号改变。结论：MRI可以作为观察正常及异常胸膜外脂肪的有效检查方法。