薄层色谱法纯化卟啉锰修饰的TFO

目的 筛选分离纯化卟啉锰-TFO-吖啶化合物的简单有效的方法。方法 以固相亚磷酰胺法合成吖啶，6碳氨基连接臂修饰的TFO，并以温和法切割和脱保护；四苯基卟啉四羧酸经金属化，酯化后，与上述TFO偶联；分别以变性聚丙酰胺凝胶电泳，寡核苷酸纯化柱，薄层色谱法分离纯化偶联反应产物。结果 以凝胶电泳分离偶联反应产物时，偶联反应产物带，卟啉锰活性酯带位于同一水平，未分离出独立的卟啉锰-TFO-吖啶化合物带，以寡核苷酸纯化柱分离偶联反应产物时，紫外分光光度仪检测结果显示，回收物仍为卟啉锰-TFO-吖啶化合物与游离的卟啉锰活性酯的混合物。以薄层层析分离偶联反应产物时，在卟啉锰活性单酯带后，出现一条绿色月牙型带，紫外光光谱分析显示，此带回收物同时具有寡核苷酸和卟啉锰的特征吸收峰（UVλmax=260,468nm)。质谱分析结果证实，回收物分子量实测值与卟啉锰-TFO-吖啶化合物理论一致。结论 薄层色谱法可简单有效分离纯化卟啉锰-TFO-吖啶化合物。     

新型尾式卟啉的制备、表征及其与DNA相互作用的初步研究

目的合成1个新型尾式卟啉化合物（目标化合物1）,并对其与小牛胸腺DNA（CT-DNA）的相互作用方式进行初步研究。方法以吡咯、对羟基苯甲醛和苯甲醛为原料,制备得到以柔性碳链连接的目标化合物1,采用MS1、HNMR以及UV-vis等对化合物进行表征;并采用紫外光谱、荧光光谱以及黏度实验初步研究目标化合物1与CT-DNA的相互作用。结果目标化合物1的电喷雾质谱（ESI-MS）在997.4出现一个归属于[M＋H]＋的分子离子峰,实验结果与理论计算一致;目标化合物1的电子吸收光谱分别在419 nm出现一个归属于卟啉环的特征Soret吸收,在500~700 nm之间出现归属于卟啉环的特征Q-带吸收。结论目标化合物1能够以插入方式与DNA分子发生结合。     

5-吡啶基-10,15,20-三甲苯基卟啉及其类似物的合成与表征

目的与方法以吡咯、对甲基苯甲醛、对甲氧基苯甲醛和醛基吡啶为原料,制备3个不对称卟啉化合物：5-（3′-吡啶基）-10,15,20-三甲苯基卟啉（1）,5-（3′-吡啶基）-10,15,20-三甲氧苯基卟啉（2）,5-（4′-吡啶基）-10,15,20-三甲氧苯基卟啉（3）。采用元素分析、电喷雾质谱、核磁共振以及电子吸收光谱和荧光发射光谱对目标化合物进行表征。结果与结论目标化合物1、2、3的电喷雾质谱（ESI-MS）分别在658.7、706.5和706.5出现一个归属于[M＋H]＋的分子离子峰,实验结果与理论计算基本一致;目标化合物1、2、3的电子吸收光谱分别在419、422和422 nm出现一个归属于卟啉环的特征Soret吸收,在450-700 nm之间出现归属于卟啉环的特征Q-带吸收。     

复方补骨脂口服液中补骨脂素的含量测定

根椐收载的验方,我们制成复方补骨脂口服液用于治疗五更泄泻、便溏腹痛。方中主要生药有补骨脂、肉豆蔻等。补骨脂温肾暖脾为君,肉豆蔻为臣,其余为佐使。补骨脂生药中所含补骨脂素为呋喃香豆素化合物,在紫外光区有吸收,在一定浓度范围内呈线性关系。本法采用薄层层析,苯—氯仿—乙酸乙脂为展开剂,展开后在紫外灯下,将补骨脂素斑点刮取,用乙醇洗脱完全,在328nm 处     

2种烷基取代吖啶酮磺酰氯紫外荧光衍生剂的合成及其光谱性质

目的以吖啶酮为原料,合成N-甲基吖啶酮-3-磺酰氯(MASC)和N-乙基吖啶酮-3-磺酰氯(EASC)2种新型紫外荧光衍生剂。方法经N位上烷基化修饰和氯磺化合成MASC和EASC,用紫外、核磁共振、质谱等技术对其进行结构表征,并将MASC、EASC与衍生剂丹磺酰氯(DNS-Cl)的紫外吸收及荧光强度相比较。结果通过紫外、核磁共振、质谱等技术证明了二者即为目标化合物,EASC、MASC与DNS-Cl的摩尔吸光系数之比分别为UVEASC-257 nm/UVDNS-Cl-323 nm=7.57、UVMASC-257 nm/UVDNA-Cl-323 nm=7.75,荧光量子效率之比分别为Φf(EASC)/Φf(DNS-Cl)=4.70、Φf(EASC)/Φf(DNS-Cl)=5.42,与二乙胺衍生的衍生物荧光强度之比分别为If(EASC)/If(DNS-Cl)=97.82、If(MASC)/If(DNS-Cl)=103.38。结论EASC和MASC有较强的紫外吸收与荧光强度,有望用于含氨基类化合物的痕量测定。     

石榴花、蒂、皮微量元素、薄层层析及紫外光谱分析

目的：通过测定石榴花、蒂、皮的微量元素含量、对照薄层层析及紫外吸收光谱，探讨其化学成分的相对含量及差异。方法：分别测定石榴花、蒂、皮微量元素含量，并作对照；对3个样品分别在两种不同展开体系中进行对照薄层层析；分别测定其紫外吸收光谱。结果：石榴的花、蒂、皮3部分的微量元素含量相差较大，其中花和皮的微量元素含量相对较高，而花中更为明显。不同展开体系的对照薄层层析显示石榴花展开后斑点较多，皮次之，蒂最少。紫外吸收光谱图在约λ220nm及λ260nm的两个主要吸收峰的吸收值，变为石榴花的最大，皮的次之，蒂的最小。结论：实验结果预示石榴花中的化学成分较为丰富，有望进一步深入研究。     

河南鼠尾草化学成分的研究

河南鼠尾草SalviahonaniaL．H．Bailey系唇形科鼠尾草属植物，主要分布在湖北、河南两省，是一种民间常用草药，其功效为活血调经，祛瘀止痛。将河南鼠尾草的丙酮提取物经氯访一水分配所得氯仿提取物，进行离心薄层层析及制备薄层层析，分得3个化合物，经鉴定分别为丹参酿-ⅡA、隐丹参酮和β-谷甾醇。所有成分均系首次从该植物中分得。1仪器与试剂熔点系用XRC－1显微熔点仅测定，未校正。紫外光谱用SchimadzuUV－2100仪测定。红外光谱用NICOLETFT－IR710仪测定。核磁共振谱用JEOL－270MHZ仪测定。质谱用JEOLD300仪测定。离心…     

异紫杉脂素的酶法糖基化

目的 以异紫杉脂素为例，探索酶法糖基化的可行性，为其他具有重要生理活性的天然化合物提供可行的生物转化方法。方法 利用半乳糖苷酶转糖基活性对异紫杉脂素进行了糖基化修饰。结果 通过薄层层析，质谱，红外光谱，^1HNMR,^13CNMR数据显示产物即是异紫杉脂素半乳糖苷。结论 E.coli和脆壁酵母来源的β-半乳糖苷酶可以对异紫杉脂素进行糖基化修饰。     

5,10,15,20—四（邻（5—氟尿嘧啶—1）乙酰苯胺）苯基卟啉的合成

报道了新型抗癌药物５，１０，１５，２０－四（邻（５－氟尿嘧啶－１）乙酰苯胺）苯基卟啉及其顺式异构体的合成方法，产率分别为１８．１％和１６．８％，这些化合物的结构经紫外光谱，红外光谱，核磁共振谱，质谱及元素分析确证。     

5,10,15,20-四[邻(5-氟尿嘧啶-1)乙酰苯胺]苯基卟啉的合成

报道了新型抗癌药物５，１０，１５，２０－四［邻（５－氟尿嘧啶－１）乙酰苯胺］苯基卟啉及其顺式异构体的合成方法，产率分别为１８．１％和１６．８％，这些化合物的结构经紫外光谱、红外光谱、核磁共振谱、质谱及元素分析确证     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…