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| 新型尾式卟啉的制备、表征及其与DNA相互作用的初步研究 | |
| 引用本文： | 蒋小磊,梅文杰,刘杰,孙冬冬,郑文杰.新型尾式卟啉的制备、表征及其与DNA相互作用的初步研究[J].广东药学院学报,2010,26(3):259-263. |
| 作者姓名： | 蒋小磊 梅文杰 刘杰 孙冬冬 郑文杰 |
| 作者单位： | 1. 广东药学院药科学院,广东广州,510006 2. 广东药学院药科学院,广东广州,510006;暨南大学生命科学院,广东广州,510632 3. 暨南大学生命科学院,广东广州,510632 |
| 基金项目： | 广东省自然科学基金博士启动基金，广东省科技计划项目资助 |
| 摘 要： | 目的合成1个新型尾式卟啉化合物（目标化合物1）,并对其与小牛胸腺DNA（CT-DNA）的相互作用方式进行初步研究。方法以吡咯、对羟基苯甲醛和苯甲醛为原料,制备得到以柔性碳链连接的目标化合物1,采用MS1、HNMR以及UV-vis等对化合物进行表征;并采用紫外光谱、荧光光谱以及黏度实验初步研究目标化合物1与CT-DNA的相互作用。结果目标化合物1的电喷雾质谱（ESI-MS）在997.4出现一个归属于M＋H]＋的分子离子峰,实验结果与理论计算一致;目标化合物1的电子吸收光谱分别在419 nm出现一个归属于卟啉环的特征Soret吸收,在500~700 nm之间出现归属于卟啉环的特征Q-带吸收。结论目标化合物1能够以插入方式与DNA分子发生结合。 |
| 关 键 词： | 尾式卟啉化合物 电子吸收光谱 荧光发射光谱 小牛胸腺DNA 黏度 |
Studies on the preparation and the interaction with calf thymus DNA of a novel tailed porphyrin |
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| JIANG Xiao-lei,MEI Wen-jie,LIU Jie,SUN Dong-dong,ZHENG Wen-jie.Studies on the preparation and the interaction with calf thymus DNA of a novel tailed porphyrin[J].Academic Journal of Guangdong College of Pharmacy,2010,26(3):259-263. | |
| Authors: | JIANG Xiao-lei MEI Wen-jie LIU Jie SUN Dong-dong ZHENG Wen-jie |
| Institution: | 1.School of Pharmacy,Guangdong Pharmaceutical College,Guangzhou,Guangdong 510006,China;2.College of Life sciencesof Chemistry,Ji′nan University,Guangzhou,Guangdong 510632,China) |
| Abstract: | Objective To synthesize a novel tailed porphyrin linked by a flexible carbon chain and study on its interaction with calf thymus DNA. Methods A novel tailed porphyrin（compound 1） was synthesized with pyrrole,p-hydroxybenzaldehyde and benzaldehyde,and was characterized by MS,1HNMR,and UV-vis.The interaction of this compound with DNA（ct-DNA） was investigated with UV,fluorospectrophy as well as viscosity experiment.Results The ESI-MS spectra of 1 had a ionic peak contributed to ＋ at 997.4.The electronic spectra of 1 had the Soret characterized absorption at 419nm and the Q characterized absorption at 500～700 nm.Conclusion The results show that 1 can bind to DNA in intercalating mode. |
| Keywords: | tailed porphyrin electronic absorption spectra fluorescence spectra ct-DNA viscosity |
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