DS-1226对慢性睡眠干扰所致小鼠抑郁行为的改善作用

目的 研究人参皂苷水解产物DS-1226对慢性睡眠干扰小鼠的抗抑郁作用,为抗抑郁药物的研发提供科学依据。方法 将72只雄性ICR小鼠分为空白对照组、模型组、阳性对照组（盐酸帕罗西汀,10 mg/kg）、DS-1226低剂量组（20 mg/kg）、中剂量组（40 mg/kg）、高剂量组（80 mg/kg）。除空白组外,其他组小鼠先进行3 d的滚筒适应,然后连续进行14 d睡眠干扰。采用体重监测、自主活动实验、悬尾实验、强迫游泳实验等实验方法,评价DS-1226的抗抑郁作用。结果 连续进行14 d睡眠干扰后,与空白对照组相比,模型组体重显著下降,悬尾和强迫游泳不动时间显著增长。与模型组相比,DS-1226中剂量组显著逆转睡眠干扰所致的体重下降,其他各给药组均未能显著逆转睡眠干扰所致的体重下降;阳性药组的悬尾不动时间极显著减少,强迫游泳不动时间有减少趋势;DS-1226中剂量悬尾不动时间显著减少,强迫游泳不动时间有明显减少趋势;DS-1226高剂量组悬尾和强迫游泳不动时间均显著减少。结论 DS-1226具有改善慢性睡眠干扰小鼠抑郁样行为的作用。     

2’,4’,6’-三羟基查尔酮衍生物合成及抗抑郁活性研究

目的 本文设计合成了11个2’,4’,6’-三羟基查尔酮衍生物,并对所合成的目标化合物进行抗抑郁活性研究,探讨其可能的作用机制。 方法 以2’,4’,6’-三羟基苯乙酮为起始原料。经过羟基保护、醇醛缩合、水解三步反应得到目标化合物3a~3k。其结构经IR、1H-NMR和MS等确证。对所合成的目标化合物采用经典抗抑郁模型“小鼠强迫游泳实验”和“小鼠悬尾实验”进行抗抑郁活性研究。采用开放性实验评价化合物3h抗抑郁活性。建立5-羟色氨酸(5-HTP)诱导小鼠甩头和育亨宾小鼠致死增强模型,观察化合物3h对诱导小鼠甩头行为和对小鼠皮下注射(sc)育亨宾致死增强的影响。 结果 实验结果表明有6个化合物在10 mg/kg剂量下均能明显缩短小鼠强迫游泳不动时间,初步断定其具有抗抑郁活性。其中化合物3h显示最好抗抑郁活性,与氟西汀相似。另外化合物3h在3种不同剂量下(10 mg/kg、20 mg/kg和40 mg/kg)均明显缩短小鼠强迫游泳和小鼠悬尾的不动时间,显示较强的抗抑郁活性,其中在20 mg/kg剂量下显示抗抑郁活性最好。化合物3h也能显著增加5-HTP诱导的小鼠甩头次数,对育亨宾诱导的小鼠致死量没有显著影响。 结论 6个查尔酮化合物显示具有明确的抗抑郁作用,其作用机制可能与5-HT系统有关。      

石菖蒲提取液的抗抑郁作用及柴胡皂苷对其作用的影响

目的:观察石菖蒲提取液的抗抑郁作用以及柴胡皂苷对其抗抑郁作用的影响。方法:选用小鼠悬尾试验、大鼠强迫游泳试验和小鼠5-HTP增强试验分别测定使用不同剂量的石菖蒲提取液、柴胡皂苷以及石菖蒲提取液加柴胡皂苷时小鼠悬尾不动时间、大鼠强迫游泳不动时间和小鼠甩头次数。结果:石菖蒲提取液能显著缩短悬尾和强迫游泳试验中小鼠和大鼠的不动时间,柴胡皂苷可加强石菖蒲提取液缩短小鼠、大鼠不动时间的作用。在小鼠5-HTP增强试验中,较大剂量(10g/kg)的石菖蒲醇沉液能增加小鼠甩头反应次数,柴胡皂苷有加强石菖蒲醇沉液增强小鼠甩头反应的作用。结论:石菖蒲提取液有明显抗抑郁作用,柴胡皂苷可加强石菖蒲提取液的抗抑郁作用。     

四逆散有效部位对小鼠行为绝望和药物抑郁模型的作用

目的研究四逆散有效部位对小鼠抑郁模型的抗抑郁作用。方法采用小鼠强迫游泳试验、悬尾试验、利血平拮抗、5羟色胺酸(5-HTP)增强等抑郁模型,观察四逆散有效部位的抗抑郁作用。结果四逆散有效部位大剂量(600 m/kg)显著缩短了小鼠强迫游泳及悬尾不动时间;四逆散有效部位大、小剂量(600、300 mg/kg)可以拮抗利血平降低小鼠体温、引起小鼠眼睑下垂的作用,明显增加5-HTP诱导小鼠的甩头次数。结论四逆散有效部位可以改善小鼠抑郁行为,具有一定的抗抑郁作用,其基本的作用机理与增加突触间单胺类神经递质,特别是5羟色胺(5-HT)的含量有关。     

小鼠腹腔水液体积检测方法建立及肾阳虚模型小鼠水负荷的评价

目的 建立小鼠腹腔水液体积的检测方法并评价肾阳虚模型小鼠的水负荷状况。方法 选取正常小鼠12只,根据“阿基米德原理”改进实验方法测量小鼠的腹腔水液体积,对其腹腔水液体积测量的稳定性进行评价;选取正常小鼠5只,利用小鼠腹腔水液检测方法对其进行线性关系考察;将SPF级昆明种雄性小鼠30只,随机分为正常组和肾阳虚模型组,肾阳虚模型组上午按0.08 mg/10 g腹腔注射苯甲酸雌二醇注射,正常组腹腔注射相同剂量的大豆油,下午两组均腹腔注射1 mL的生理盐水造成水负荷,连续15 d。结果 该测量方法的稳定性和线性关系考察均取得了良好的效果;与正常组相比,造模后体重显著性降低（P<0.01）,肛温显著性降低（P<0.01）,趾温差异无显著性（P >0.05）;自主活动明显减少（P<0.05）;游泳时间明显降低（P<0.01）,综合评价说明肾阳虚造模成功;肾阳虚模型组的腹腔水液测量体积整体表现出下降的趋势,腹水指数显著高于正常组（P<0.05）。结论 该检测方法可以快速、准确的测量出小鼠腹腔水液测量体积和腹水指数的变化,可以能较客观地评价中医证候的特点,为中医证候症状描述进一步提供实验数据支持。     

不同条件环磷酰胺建立小鼠免疫力低下模型的比较及偏最小二乘法（PLS）数学建模分析

目的 通过比较4种不同剂量、周期的环磷酰胺建立小鼠免疫力低下模型,并采用偏最小二乘法(PLS)建模分析筛选出最优方案。 方法 雄性昆明小鼠58只随机分成5组,分别为正常组10只,每日注射生理盐水;模型1组12只,按照40 mg/kg的剂量连续注射环磷酰胺溶液10 d;模型2组12只,按照80 mg/kg的剂量连续注射环磷酰胺溶液3 d;模型3组12只按照40 mg/kg的剂量注射2次环磷酰胺溶液,每次间隔(2~3)d;模型4组12只按照50 mg/kg的剂量连续注射环磷酰胺溶液7 d。监测不同剂量和不同周期给予小鼠环磷酰胺后,模型小鼠的饮食、饮水、体重等日常代谢指标的变化;测定小鼠胸腺、脾等脏器指数以及血常规等免疫指标的变化;采用SIMCA-P软件处理小鼠的终体重、终肛温、脏器指数、血常规等指标,应用偏最小二乘法(PLS)综合评价免疫力低下小鼠模型的建立。结果 与正常组相比,模型组小鼠体重和肛温降低且差别有显著性意义(P<0.05);日平均饮食、饮水量降低且差异有显著性(P<0.01);脾指数和胸腺指数降低且差别有极显著性意义(P<0.01);模型组嗜碱细胞绝对值和嗜碱细胞百分比均降低且差异有显著性(P<0.05),模型组平均RBC血红蛋白浓度、大血小板比率均升高且有显著性差异,模型1组、模型2组、模型4组的白细胞、淋巴细胞绝对值均降低且差异有显著性(P<0.05)。PLS分析处理表明,模型1组、模型2组、模型4组与正常组比较差异都具有显著性(P<0.01),其中模型1组差异最大。结论 通过PLS分析方法综合不同指标,模型1组按照40 mg/kg的剂量连续注射10 d为最佳造模方法,为建立稳定的免疫力低下模型提供实验依据。     

新型5-HT1A受体激动和5-HT重摄取抑制双重活性化合物YL-0919抗抑郁作用的行为学评价

目的 研究兼有5-HT_1A受体激动和5-HT重摄取抑制双重活性化合物YL-0919的抗抑郁作用。方法 分别采用小鼠悬尾实验、小鼠强迫游泳实验、小鼠自发活动实验和大鼠获得性无助模型,观察不同剂量（0.625、1.25、2.5和5 mg/kg）YL-0919的抗抑郁作用。结果 在小鼠悬尾实验和小鼠强迫游泳实验中,单次灌胃给予YL-0919（0.625～2.5 mg/kg）能够显著缩短小鼠悬尾不动时间和游泳不动时间;在小鼠自发活动实验中,YL-0919在上述剂量范围对自发活动无影响;在大鼠获得性无助模型上,灌胃给予YL-0919〔0.625～1.25 mg/（kg·d）,1～4 d〕能显著减少逃避失败次数。结论 YL-0919在小鼠和大鼠模型上具有明确的抗抑郁活性,并且在抗抑郁的有效剂量范围内无中枢兴奋和抑制作用,具有成为新型抗抑郁药物的研发潜力。     

交泰丸对利血平诱导小鼠抑郁模型的影响

[目的] 探讨交泰丸对利血平诱导小鼠抑郁模型的影响。[方法] 采用小鼠强迫游泳实验（FST）、小鼠悬尾实验（TST）和小鼠利血平拮抗实验,通过高效液相色谱（HPLC）测定小鼠海马和皮质组织中单胺类神经递质[去甲肾上腺素（NE）、5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）]含量的变化,探讨交泰丸的抗抑郁作用。[结果] 交泰丸组方各剂量组均能明显减少FST和TST的不动时间,并且均能拮抗利血平诱导的小鼠眼睑下垂和体温下降,而且可以逆转NE和5-HT的减少以及降低5-HIAA的含量。[结论] 交泰丸具有明显的抗抑郁作用,并且可以通过调节海马组织和皮质中NE和5-HT的含量而发挥抗抑郁作用。     

雌激素诱导SD大鼠前列腺炎与内环境改变的关系

目的 通过雌二醇诱导SD雄鼠慢性前列腺炎,探讨SD雄鼠的内环境改变的关联性作用,为前列腺炎发生机制和治疗提供一条实验性途径。方法 3%戊巴比妥麻醉,无菌条件下去势手术;术后动物恢复5 d,30只雄鼠按照体重随机分组,每组10只。第6天低剂量组皮下注射0.25 mg/kg的雌二醇,高剂量组皮下注射1.25 mg/kg的雌二醇,溶媒对照组注射橄榄油,每天1次,连续30 d。结果 与溶媒对照组比较,低、高剂量组白细胞（WBC）、红细胞（RBC）、血红蛋白（HGB）和红细胞压积（HCT）均降低（P<0.01）;前列腺酸性磷酸酶（PAP）明显降低（P<0.01）;高剂量组睾酮含量明显增加（P<0.01）,C-反应蛋白（CRP）在低、高剂量组也呈现增加的趋势,但差异没有统计学意义;溶媒对照组和给药组前列腺组织大体解剖表面未见明显差异,光学显微镜下,溶媒对照组前列腺组织结构完整、腺腔和间质无炎性细胞浸润,高剂量组和低剂量组的前列腺间质均可见炎细胞浸润;低、高剂量组大鼠脾脏均可见棕黄色颗粒沉淀,其余脏器无明显改变,胸腺、脾脏及其脏器系数均明显降低。结论 根据本实验结果,雌激素引起的慢性非细菌性前列腺炎可导致内环境血液学、血清生化,激素水平、脏器和脏器系数的一系列改变。     

罗布麻叶总黄酮抗抑郁作用及其机制研究

目的 探讨罗布麻叶总黄酮的抗抑郁作用及与多巴胺能系统相关的可能机制。方法 采用经典的小鼠强迫游泳和悬尾抑郁模型,观察罗布麻叶总黄酮的抗抑郁作用。同时观察多巴胺D₁受体阻滞剂SCH23390、D₂受体阻滞剂舒必利对小鼠强迫游泳和悬尾试验中罗布麻叶总黄酮抗抑郁作用的影响,研究罗布麻叶总黄酮抗抑郁的作用机制。结果 罗布麻叶总黄酮具有明确的抗抑郁作用;SCH23390、舒必利与罗布麻叶总黄酮联合ig给药10 d,能明显延长悬尾小鼠累计不动时间（P＜0.05）。结论 罗布麻叶总黄酮的抗抑郁作用与多巴胺能系统有关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.