槲皮素对药物致心肌细胞损伤的保护作用

目的:探讨槲皮素(QUE)对柔红霉素(DNR)诱导的体外培养原代SD乳鼠心肌细胞损伤的保护作用.方法:分离培养心肌细胞,分为空白对照组、DNR组、DNR QUE组、QUE组,孵育24 h,显微镜下观察心肌细胞形态,检测培养介质中乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、脂质过氧化产物丙二醛(MDA)的含量.结果:与DNR组比,DNR QUE组脱落细胞减少,细胞碎片减少,LDH和MDA含量降低(P＜0.05),SOD活性增高(P＜0.05),且有剂量效应关系.结论:25～100 μmol/L浓度范围的槲皮素能减轻柔红霉素对心肌细胞的损伤,具有细胞保护作用,机制可能与其抗氧化作用有关.     

蜂胶对乳鼠心肌细胞氧化损伤的保护作用

目的:探讨蜂胶(propolis)对乳鼠心肌细胞氧化损伤的作用及其可能机制。方法:建立乳鼠心肌细胞过氧化氢(H2O2)损伤模型,通过观察细胞形态及细胞存活率,检测培养液中乳酸脱氢酶(LDH)活性、超氧化物歧化酶(SOD)活性及脂质过氧化产物丙二醛(MDA)的含量,观察不同浓度蜂胶(50、100、200 mg/L)对乳鼠心肌细胞氧化损伤的影响。结果:不同浓度的H2O2(50、100、200、400、800μmol/L)孵育乳鼠心肌细胞4 h后,心肌细胞存活率随H2O2浓度增加而降低,呈剂量依赖性;细胞形态出现明显损伤改变,心肌细胞伪足回缩、体积变小、自律搏动减弱。与模型组比较,经蜂胶预处理心肌细胞培养上清液中LDH释放量、MDA生成量降低,SOD活性提高;细胞存活率显著改善,差异有统计学意义(P<0.05)。结论:蜂胶对H2O2所致的乳鼠心肌细胞氧化损伤有明显的保护作用,其机制可能是通过抑制脂质过氧化,增强心肌细胞膜抗氧化能力所致,至于其确切机制有待进一步探讨。     

对乙酰氨基酚对心肌细胞H2O2损伤的保护作用

目的 研究对乙酰氨基酚(acetaminophen,APAP)对H2O2引起的心肌细胞损伤的保护作用.方法 原代SD大鼠乳鼠心肌细胞培养,48 h后加入H2O2造成心肌细胞损伤,损伤前1 h加入不同浓度的APAP作为保护剂.损伤6 h后,甲基四唑蓝比色(MTT)法测定线粒体脱氢酶活性,试剂盒测定培养液中乳酸脱氢酶(LDH)活性、心肌细胞中脂质过氧化产物丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、Caspase-3活性.结果 H2O2能明显造成心肌细胞损伤, 表现为:细胞活力降低、LDH活性增加、MDA含量增加、SOD活性下降、caspase-3活性增高;20 μmol·L-1、60 μmol·L-1、180 μmol·L-1的APAP对H2O2引起的心肌细胞损伤有保护作用,并随浓度增加保护作用加强.结论 APAP能减轻H2O2对心肌细胞的损伤作用,抑制H2O2引起的培养心肌细胞凋亡.     

左卡尼汀对心肌细胞H2O2损伤的保护作用

目的 研究左卡尼汀对H2O2引起的心肌细胞损伤的保护作用.方法 利用体外培养乳鼠心肌细胞,0.2 mM H2O2作用12 h建立氧化应激损伤模型,观察不同浓度左卡尼汀(50,100,200 mg/L)对心肌细胞活力、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性的影响.结果 与正常组比较,模型组细胞活力下降,细胞MDA含量增加,SOD活性下降(P＜0.01);与模型组比较,不同浓度的左卡尼汀可以提高心肌细胞活力,降低细胞MDA含量,增加SOD活性.结论 左卡尼汀对H2O2诱导的乳鼠心肌细胞损伤有保护作用.     

藏药八味沉香散对H_2O_2所致乳鼠心肌细胞氧化应激损伤的保护作用

目的探讨八味沉香散对过氧化氢（H2O2）所致乳鼠心肌细胞氧化应激损伤的保护作用。方法 SD乳鼠心肌细胞原代培养,H2O2诱导心肌细胞氧化应激损伤,用不同剂量藏药八味沉香散处理24 h后,测定培养液中乳酸脱氢酶（LDH）、肌酸激酶（CK）、过氧化氢酶（CAT）的含量;同时检测培养液中超氧化物歧化酶（SOD）、丙二醛（MDA）及谷胱甘肽过氧化物酶（GSH-Px）的活性。结果八味沉香散可明显减轻H2O2所致乳鼠心肌细胞培养液中LDH、CK和CAT的释放量,同时能够升高SOD、GSH-Px活性,降低MDA活性。结论八味沉香散对H2O2所致乳鼠心肌细胞氧化应激损伤有明显保护作用。     

镁离子对培养乳鼠心肌细胞羟自由基损伤的保护作用

目的　观察镁离子对培养乳鼠心肌细胞羟自由基损伤的保护作用。方法　本实验利用FeSO4/ H2O2 自由基产生体系,观察羟自由基作用于培养乳鼠心肌细胞,其上清液中乳酸脱氢酶(LDH) 和丙二醛( MDA) 含量的变化,以及镁离子对这种作用的影响。结果　(1) 羟自由基作用于培养乳鼠心肌细胞,其上清液中LDH 和MDA 的含量增加。(2) 镁离子能减少羟自由基引起的LDH 和MDA 含量的增加,且呈一定浓度、时间依赖关系。结论　羟自由基使培养乳鼠心肌细胞LDH 漏出增加及脂质过氧化终产物MDA 含量增加,镁离子对这一损伤有保护作用     

五味子乙素对乳鼠缺氧/复氧损伤心肌细胞的保护作用及其机制

目的：探讨五味子乙素(Sch B)预处理对乳鼠心肌细胞缺氧/复氧（H/R）损伤的影响,并探讨其可能机制。方法：分离培养原代乳鼠心肌细胞,将其分为对照组、模型组和10、50、250 mg/LSch B预处理组,除对照组外各组细胞采用2 mmol/L  Na2S2O4培养2 h后换用正常培养液培养18 h,造成心肌细胞H/R损伤。倒置显微镜观察各组心肌细胞形态学变化,MTT法检测各组细胞存活率,检测各组细胞中乳酸脱氢酶（LDH）、肌酸激酶（CK）和超氧化物歧化酶（SOD）活性及丙二醛（MDA）水平。 结果：与对照组比较,模型组细胞明显回缩、停搏或浮起,细胞存活率明显降低（P<0.01）,LDH和CK活性及MDA水平升高（P<0.01）,SOD 活性降低（P<0.01）;与模型组比较,SchB预处理各组细胞博动尚存,回缩较轻,细胞存活率明显升高（P<0.01）,LDH 和CK活性及MDA水平降低（P<0.01）,SOD 活性升高（P<0.05或P<0.01）。 结论：Sch B对乳鼠H/R损伤的心肌细胞具有保护作用,其机制可能与其抗脂质过氧化作用有关。     

木犀草素-7-O-β-D-葡萄糖苷对H_2O_2诱导乳鼠心肌细胞损伤的保护作用

目的:观察木犀草素-7-O-β-D-葡萄糖苷对过氧化氢(H2O2)诱导乳鼠心肌细胞氧化损伤的作用及其机制。方法:木犀草素-7-O-β-D-葡萄糖苷预作用12 h后,采用H2O2诱导培养乳鼠心肌细胞氧化损伤模型,测定细胞存活率、细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量、细胞内活性氧(ROS)水平及细胞内钙(Ca2+)超载的变化。结果:木犀草素-7-O-β-D-葡萄糖苷能明显提高H2O2损伤细胞存活率,提高细胞内SOD活性,降低胞内MDA和ROS生成量,同时使细胞内钙含量降低。结论:木犀草素-7-O-β-D-葡萄糖苷对H2O2氧化损伤心肌细胞具有明显的保护作用,机制可能与其抑制脂质过氧化、抑制细胞内钙超载有关。     

缬沙坦对乳鼠心肌细胞过氧化损伤的保护作用

目的:探讨缬沙坦对过氧化氢(H2O2)致心肌细胞过氧化损伤的保护作用.方法:分离培养SD乳鼠心肌细胞,建立心肌细胞过氧化损伤模型.用2～10 μmol/L缬沙坦预处理后,测定各组心肌细胞存活率;用10μmol/L缬沙坦预处理后,检测各组心肌细胞培养介质中乳酸脱氢酶(LDH)、丙二醛(MDA)及超氧化物歧化酶(SOD)浓度.结果:在2～10 μmol/L范围内,缬沙坦剂量依赖性地提高心肌细胞过氧化损伤的细胞存活率(P＜0.05);10μmol/L缬沙坦预处理后,培养介质中LDH和MDA的生成减少(P＜0.05),而SOD含量增高(P＜0.05).结论:缬沙坦可能通过稳定细胞膜、抗脂质过氧化反应及提高清除氧自由基,对心肌心肌细胞过氧化损伤起剂量依赖性保护作用.     

银杏叶总提取物对H2O2诱发的心肌细胞损伤的保护作用

目的研究银杏叶总提取物(GBE)对H2O2诱发的心肌细胞损伤的保护作用。方法采用胰酶消化法获取大鼠乳鼠心肌细胞,用H2O2损伤心肌细胞,制备心肌缺血再灌注损伤实验模型,实验组加入GBE使其终质量浓度分别为50、100、150 mg/L,阳性对照组加入异博定,用紫外分光光度法、硫代巴比妥酸法、黄嘌呤氧化酶法分别测定细胞培养液中乳酸脱氢酶(LDH)活性、丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性;测定线粒体中琥珀酸脱氢酶(SDH)、细胞色素C氧化酶(CCO)比活力;透射电镜观察细胞的超微结构。结果与对照组相比模型组LDH活性和MDA水平均明显升高(P<0.05、0.001),SOD则明显降低(P<0.01),而GBE各组能减少LDH和MDA的产生呈剂量依赖关系;SDH和CCO比活力在H2O2组明显下降(P<0.001),GBE组改变不明显;150 mg/L GBE对细胞线粒体有明显的保护作用。结论银杏叶提取物对H2O2诱发的心肌细胞损伤有显著的保护作用,其机制可能与维持细胞中线粒体的能量代谢有关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.