长效局麻药对中性粒细胞呼吸爆发的抑制作用

目的观察几种长效局麻药对多形核中性粒细胞(PMN)释放氧自由基的抑制作用。方法选取近2周未用过任何药物的健康志愿者12人,抽取静脉血经分离得PMN悬液。将每人的血样分成6组:Ⅰ、Ⅱ组分别为单纯的反应混合物和参考样品,含有不同局麻药(浓度均为1×10-4)的Ⅲ~Ⅵ组为实验组。以fMLP或PMA激活反应,用细胞色素C减少法测定活化PMN产生的细胞外O2-含量的变化。Ⅰ,Ⅱ两组光密度之差作为对照值,各实验组吸光度与Ⅱ组吸光度之差为依赖于O2-的细胞色素C减少值,以反映PMN的O2-释放。结果未加局麻药的PMN悬液O2-的释放量为9.3±4.7nmol/106个细胞。而含有罗哌卡因、S(-)布比卡因、布比卡因和地卡因的PMN悬液O2-的释放量分别是对照值的77.5%、75.8%、88.3%和61.3%(P<0.01)。结论地卡因、罗哌卡因、布比卡因和S(-)布比卡因对活化的PMN氧自由基的释放有不同程度的抑制作用,不同局麻药之间的差异可能与药物的立体异构和脂溶性有关。     

红花黄色素对乳腺癌细胞增殖和迁移的抑制作用及其分子机制

目的 研究红花黄色素 (CY) 对乳腺癌细胞增殖、凋亡、迁移和侵袭的影响以及相关的分子机制.方法 采用CCK-8法检测不同浓度和时间红花黄色素对人乳腺癌细胞MDA-MB-231的抑制作用;采用流式细胞术检测不同浓度红花黄色素对人乳腺癌细胞MDA-MB-231的促凋亡作用;采用Transwell法检测不同浓度红花黄色素对人乳腺癌细胞MDA-MB-231迁移和侵袭的影响;在不同浓度的红花黄色素的干预后, 通过western blot技术检测凋亡相关蛋白Cleaved-Caspase-3和生存相关蛋白p-Akt以及转移相关蛋白MMP2的表达.结果 (1) 红花黄色素对乳腺癌细胞的抑制作用具有浓度依赖性和时间依赖性; (2) 不同浓度的红花黄色素干预能显著促进乳腺癌细胞的凋亡 (P<0.01) ; (3) 不同浓度的红花黄色素干预能显著抑制p-Akt的表达并同时促进Cleaved-Caspase-3的表达; (4) 不同浓度的红花黄色素能够显著抑制乳腺癌细胞的迁移和侵袭能力 (P<0.01) , 并且能够抑制迁移相关蛋白MMP2的表达.结论 红花黄色素能在体外通过激活凋亡通路而促进凋亡, 并能通过抑制MMP2来抑制乳腺癌细胞的转移.红花黄色素是一种潜在的治疗乳腺癌的药物.     

葶苈子黄酮对血小板激活因子的拮抗作用

目的研究葶苈子黄酮对血小板激活因子(PAF)诱导的家兔中性粒细胞(PMN)和洗涤家兔血小板(WRP)的聚集及黏附作用的影响。方法比浊法观察PAF诱导的PMN(或WRP)聚集;PAF诱导已着色PMN(或WRP)黏附,比色法测定此PMN(或WRP)破膜后溶液吸光度,表示其黏附活性。结果葶苈子黄酮可明显抑制PAF诱导的PMN和WRP聚集及黏附活性的升高(P<0.05)。结论葶苈子黄酮具有拮抗PAF的作用。     

异普复合静脉麻醉对IL-6,CD18,SOA的影响

目的 :研究异丙酚或 /和普鲁卡因对佛波酯 (PMA)刺激下离体中性粒细胞 (PMN)表面粘附分子CD18表达、超氧化物阴离子 (SOA)释放及内毒素 (LPS)刺激下全血中IL - 6生成的影响。方法 :提纯PMN并用磷酸盐缓冲液 (PBS)制成悬液 ,样本随机分为 5组 :空白组 (PMN PBS) ,损伤组 (PMN PBS PMA 10 0ng/ml) ,异丙酚组 (PMN PMA 10 0ng/ml propofol 4 μg/ml) ,普鲁卡因组 (PMN PMA10 0ng/ml procaine 15μg/ml)及异普复合组 (PMN PMA 10 0ng/ml 2 μg/mlpropofol 8μg/mlprocaine)。另设相应浓度药物组。流式细胞仪检测CD18表达 ,细胞色素C还原法测定SOA释放 ,放免法测定全血中IL - 6生成。结果 :异丙酚、普鲁卡因单独或联合应用均可抑制中性粒细胞表面粘附分子CD18表达及SOA释放 ,异丙酚抑制效应强于普鲁卡因。异普复合组效应与异丙酚临床浓度组接近 ,强于普鲁卡因临床浓度组。异丙酚促进全血中IL- 6的生成 ,普鲁卡因抑制全血中IL - 6的生成 ,异普复合组作用接近普鲁卡因组。结论 :异丙酚普鲁卡因复合应用对抑制中性粒细胞粘附功能 ,减轻组织损伤作用具有明显的互补性 ,这为两种麻醉药物在临床实践中复合应用的合理性给予基础实验支持     

超声法提取红花中红花黄色素的工艺研究

目的研究超声法提取红花药材中红花黄色素的最佳工艺。方法以单因素实验分析了提取溶剂浓度、提取时间及料液比3个因素对红花黄色素提取率的影响。在单因素实验基础上，通过正交实验考察超声法提取的最佳工艺。结果影响超声法提取红花黄色素的因素中只有乙醇浓度对红花黄色素提取率的影响有显著性。最佳提取工艺为以50％乙醇溶液为提取溶剂，料液比1：10，超声提取60min。结论该方法采用全物理过程，无任何污染，是提取红花中红花黄色素的有效途径。     

烧伤后PMN粘附对肺微血管内皮细胞[Ca2+]i的影响

严重烧伤后早期肺组织微血管内中性粒细胞的粘附贴壁能使肺血管通透性增加[1],而这种改变则依赖于内皮细胞胞内钙离子浓度([Ca2+]i)的增加[2].本实验观察烧伤血清及烧伤后中性粒细胞(PMN)对肺微血管内皮细胞(PMEC)[Ca2+]i的影响,并探讨PMN粘附及其粘附分子CD11b/CD18在该影响中的介导作用.     

白细胞介素-10对小鼠中性粒细胞吞噬和杀菌活性的影响

目的: 探讨白细胞介素-10(IL-10)对中性粒细胞(PMN)吞噬和杀菌活性的影响,以了解IL-10对非特异性免疫的作用机制。方法: 小鼠麻醉后从眼球取血,与不同浓度IL-10在37℃水浴中作用2 h,然后加FITC标记大肠埃希菌,置37℃水浴中15 min,溶血剂去除红细胞,流式细胞仪检测各IL-10浓度组PMN的吞噬率。小鼠麻醉后局部消毒,摘眼球,无菌方法取血。与不同浓度IL-10在37℃下作用2 h,然后加大肠埃希菌置37℃水浴1 h,同时设PMN杀菌0 min组、无IL-10的PMN杀菌对照组,各组样品作适当稀释后接种琼脂平板,最后观察各组菌落数,与对照组比较,计算IL-10对杀菌力影响。结果: PMN的吞噬功能在IL-10浓度为1 ng/ml组减低(15.87 ±14.25)%,但差异无统计学意义(P > 0.05)。而PMN的杀菌功能在IL-10浓度为0.1 ng/ml组降低(39.59 ±37.36)%,在IL-10浓度为1 ng/ml组则降低(42.28 ±22.24)%,差异均有统计学意义(P <0.05)。结论: IL-10对PMN的吞噬功能有轻度的抑制作用,对PMN的杀菌功能有明显的抑制作用,对非特异性免疫亦有抑制作用。     

BN52021对血小板活化因子引起的银屑病患者中性白细胞聚集反应的影响

据《中华医学杂志》1994年74卷第6期报道沈阳军区总医院皮肤科刘宝军等,采用聚集仪测定了血小板活化因子(PAF)拮抗剂BN52021对PAF引起银屑病患者嗜中性白细胞(PMN)聚集反应的影响。 结果表明,PAF对PMN有较强的聚集作用,并呈剂量依赖关系。银屑病患者PMN对低浓度PAF聚集反应明显高于正常人(P<0.05),而对高浓度PAF     

乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用

目的 研究乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用及其机制.方法 体外培养的人脐静脉内皮细胞(HUVECs)通过肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)孵育后活化,正常人血液分离的中性粒细胞经TNF-α孵育后激活,活化的HUVECs与激活的中性粒细胞共孵育建立中性粒细胞介导的内皮细胞损伤体外模型.共孵育体系中加入不同浓度的乌司他丁(100、500、1 500、3 000和5 000 U/ml),MTT法检测内皮细胞存活率,同时检测培养液中乳酸脱氢酶活性(LDH)、一氧化氮(NO)含量和弹性蛋白酶活性.中性粒细胞与TNF-α及不同浓度的乌司他丁共孵育后,流式细胞仪检测细胞内弹性蛋白酶活性.结果 激活的中性粒细胞与活化的HUVECs共孵育可以显著降低内皮细胞存活率,同时培养液中LDH活性显著升高、NO含量显著降低、弹性蛋白酶活性显著升高,乌司他丁可以剂量依赖性对抗上述变化.同时,乌司他丁可以剂量依赖性降低TNF-α激活的中性粒细胞内弹性蛋白酶的活性.结论 乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用具有剂量依赖性,其机制与乌司他丁抑制中性粒细胞内及释放到细胞外的弹性蛋白酶的活性有关.     

酸浆苦素B对活化多形核中性粒细胞化学发光及H_2O_2产生的影响

通过观察化学发光及检测H2O2产生，考察锦灯笼的主要成分酸浆苦素B（PhysalinB，PhB）对调理酵母多糖激活的多形核中性粒细胞（polymorphonuclearleukocyte，PMN）的影响。结果显示，不同浓度的PhB对活化的PMN化学发光呈剂量相关的抑制作用，同时对活化PMNH2O2的产生也有相同趋势的抑制作用。提示锦灯宠抗炎机理可能是抑制PMN氧自由基的产生和释放而发挥作用。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…