牛颈静脉带瓣管道重建犬右室-肺动脉连接后的钙化研究

目的研究牛颈静脉用于重建右室-肺动脉连接后的钙化情况。方法以戊二醛鞣制的牛颈静脉带瓣管道为研究对象,建立犬重建右心室-肺动脉连接动物模型,观察9￣10个月,通过原子光谱吸收法测定组织钙含量,以评价在体内循环系统中与血流接触的情况下,牛颈静脉带瓣管道的钙化情况。结果管壁钙含量为(22.05±10.78)μg/mg,瓣膜钙含量为(0.88±0.69)μg/mg,后者仅为前者的3.99%,经配对t检验分析,在统计学上差异均有显著性(P<0.001)。瓣下管壁钙含量为(16.04±4.25)μg/mg,瓣上管壁钙含量为(28.07±17.98)μg/mg,配对t检验显示二者在统计学上差异无显著性(P>0.05);瓣膜的基底部、交界部及游离缘3者之间的钙含量在统计学均无显著性差异,配对t检验显示P>0.05;结论牛颈静脉带瓣管道重建右室-肺动脉连接后,血管壁远较瓣膜容易钙化;瓣上管壁和瓣下管壁钙化程度无明显差异,瓣膜各部分钙化程度无明显差异。     

辛醇对戊二醛鞣制的牛心包抗钙化改性的实验研究

目的:探索戊二醛鞣制牛心包生物瓣膜新的处理方法,以延缓其钙化进程,从而延长牛心包生物瓣使用寿命。方法:用辛醇对戊二醛鞣制的牛心包进行改性处理,通过建立大鼠皮下包埋动物模型加速体内钙化的实验方法;应用原子吸收光谱法测定组织钙含量,以评价辛醇化学改性对戊二醛鞣制的牛心包钙化的影响。结果:辛醇组和戊二醛牛心包钙含量分别为(113．19±14．86)μg／mg和(256．16±21．52)μg／mg(P< 0．001),辛醇组牛心包试片钙含量明显低于戊二醛组。结论:在戊二醛鞣制的基础上通过辛醇对牛心包进行进一步的化学玫性处理,可显著延缓牛心包的钙化进程,可望成为一种增强牛心包生物瓣耐久性的有效方法。     

戊二醛固定的牛颈静脉带瓣管道的钙化实验研究

目的检测戊二醛固定的牛颈静脉带瓣管道的在体钙化程度,评价用戊二醛固定牛颈静脉带瓣管道的可行性,为寻找最佳的固定方法提供基础资料.方法将戊二醛处理后的牛颈静脉管壁及瓣叶组织用肝素进行处理,植入脱乳的SD大鼠背部皮下,60 d后取出,进行自身对照,采用原子吸收分光光度法检测组织钙含量,HE染色、Von-Kossa染色进行形态学检测,透射电镜观察超微结构变化.结果包埋前的牛颈静脉瓣膜、管壁试片平均钙含量分别为(1.84±0.75)、(0.53±0.22)μg/mg干重,包埋后的牛颈静脉瓣膜、管壁试片平均钙含量分别为(16.23±8.32)、(151.00± 23.78)μg/mg干重,两组相比有显著差别(P＜0.001).光镜及电境下显示包埋前的牛颈静脉瓣膜、管壁内皮保存完整,组织结构紧凑,胶原纤维结构保持良好;包埋后的牛颈静脉瓣膜、管壁则内皮残缺,结构疏松,胶原纤维破坏严重.结论戊二醛交联的牛颈静脉带瓣管道,可见明显钙化.瓣膜钙化较轻,管壁钙化严重.     

阳离子油复合鞣制方法处理人工生物瓣膜生物材料的研究及临床应用

目的:本研究对牛心包瓣膜生物材料戊二醛鞣制与阳离子油复合鞣制方法进行了对比，从分子水平上对生物材料鞣制方法的机制进行了研究。方法：对牛心包生物材料进行组织学、超微结构和机械抗张强度测试。对比阳离子油复合鞣制牛心包和戊二醛鞣制牛心包组织的钙含量；用傅立叶红外光谱仪测定羧基含量；对牛心包生物瓣膜进行体外模拟疲劳实验台加速检测。结果：阳离子油复合鞣制牛心包瓣10年后扫描电镜观察，组织胶原纤维结构排列致密、整齐，细胞结构完整、牢固，钙化不明显。阳离子油复合鞣制组牛心包钙含量(2.87±0.32)μｇ/ｍｇ，戊二醛组牛心包钙含量(9.82±4.45)μｇ/ｍｇ，两者差异有统计学意义 (P＜0.05)。阳离子油复合鞣制组，收缩温度在86～90?℃时，牛心包生物材料最柔软，抗张强度19.9～25.8?Ｎ/ｍｍ2、撕裂强度92.3～112.7?Ｎ/ｍｍ2、延伸率43.4%～46.0%。阳离子油复合鞣制组，红外光谱图显示羧基（COOH-1）峰明显降低，而戊二醛鞣制组仍含有相当多的羧基。体外模拟加速疲劳实验台测试，牛心包瓣膜为3.8亿次，从受力疲劳方面能经受住大约10年的寿命。结论：应用阳离子油复合鞣制牛心包生物瓣膜，克服了戊二醛鞣制使生物组织变硬变脆及钙化缺陷，提高了生物组织的柔软度和强度，且有防钙化作用，能显著延长其使用寿命。     

戊二醛结合肝素鞣制牛颈静脉带瓣管道抗钙化的实验研究

目的用动物皮下包埋的模型,用戊二醛鞣制的牛颈静脉带瓣管道(VBJVC)作为对照,来研究戊二醛结合肝素鞣制的牛颈静脉带瓣管道是否具有更好的抗钙化能力和抗衰老能力。方法把牛颈静脉带瓣管道分为两组,实验组(戊二醛结合肝素组)和对照组(戊二醛组),分别包埋在两组实验兔的皮下。90d后取出牛颈静脉带瓣管道片,进行钙的定量分析和形态学检查。形态学分析包括光镜下HE染色和Von-Kossa染色,进行钙化程度的比较。结果比较戊二醛结合肝素鞣制的VBJVC(A组)较之传统的单纯的用戊二醛鞣制的VBJVC(B组),其结果有明显的统计学意义。(1)标本用原子分光光度计测量组织钙含量,A组(13.92±5.03)mg/g,B组(34.99±12.33)mg/g,A组的组织钙化明显低于B组;(2)光镜下用Von-Kossa钙染色,按照Carpentier的分级方法,A组,0度2个,Ⅰ度3个,Ⅱ度1个。B组,0度1个,Ⅰ度1个,Ⅱ度2个,Ⅲ度2个,A组的组织钙化明显低于B组。结论经过戊二醛结合肝素鞣制的VBJVC,较之单纯用戊二醛鞣制的VBJVC,有较好的抗钙化能力。     

经戊二醛固定的牛颈静脉带瓣管道改性方法评价

目的:改性后的牛颈静脉带瓣管道植入犬的右室流出道,观察其长期的防钙化性能.方法:从屠宰场取新鲜牛颈静脉,筛选后放入循环固定槽内,用体积分数为0.625%的戊二醛固定1 d,取出分为2组,分别于体积分数为100%2,3-丁二醇和体积分数为0.3%戊二醛中保存60 d,然后分组植入犬的右室-肺动脉之间,将犬的肺动脉近端结扎,饲养1 a后行牛颈静脉带瓣管道的组织病理学观察及钙含量测定.结果:2,3-丁二醇保存组管道内腔及瓣膜表面发现有内皮样细胞存在.戊二醛保存组较2,3-丁二醇组管道组织均存在较严重的炎性反应.2,3-丁二醇改性后的牛颈静脉管壁平均钙含量((8.72±0.71)mg/g)明显比戊二醛组((22.05±10.78)mg/g)低,P＜0.05;2组瓣膜平均钙含量相比(2,3-丁二醛组:(1.38±0.86)mg/g),戊二醛组:((0.88±0.69)mg/g)差异无统计学意义(P＞0.05).结论:戊二醛鞣制的生物组织材料经2,3-丁二醇改性后可明显减轻组织钙化程度.     

染料介导光氧化法处理的同种异体动脉体内近期抗钙化性能评价

目的：观察染料介导光氧化法（DMP）处理的家兔胸主动脉用于同种异体腹主动脉置换后，在体内血流环境下的抗钙化性能。方法：以经染料介导光氧化反应处理的家兔胸主动脉为研究对象，以戊二醛(GA)处理的家兔胸主动脉以及自体腹主动脉原位移植(OT)作为对照，建立家兔胸-腹主动脉移植模型；实验动物观察饲养1个月后，取出标本，通过原子吸收光谱法测定血管组织钙含量，扫描电镜观察血管壁钙化情况。结果：DMP组血管壁钙含量为(3.58±0.22)μg/mg，GA组血管壁钙含量为(22.5±0.53)μg/mg，自体动脉移植组组织钙含量(2.99±0.33)μg/mg，DMP组组织钙含量虽较OT组高，但差异无统计学意义（P＞0.05），DMP组组织钙含量较GA组低（P＜0.01）；扫描电镜下观察可见，GA组有大量的钙沉积，DMP组仅见少量钙沉积。结论：光氧化反应处理的家兔胸主动脉在家兔胸 腹主动脉移植的动物模型中，抗钙化性能优于戊二醛固定的胸主动脉。     

醇对戊二醛鞣制的牛心包防钙化改性的实验研究

目的本实验采用几种醇改性戊二醛处理后的牛心包,希望寻找一种理想的生物瓣处理方法。方法分为五组,即新鲜对照组及戊二醛、2,3-丁三醇、1,3-丙二醇、1,2-丙二醇组。分组将牛心包试片植入SD大鼠背部皮下60d后,行组织钙定量测定及组织学检测。结果2,3-丁二醇改性后的牛心包试片钙平均含量为3.99±0.96ug/mg干重,与GA组、1,2-丙二醇,1,3-丙二醇试片的钙平均含量(241.89±7.79、244.26±6.39、167.32±12.29ug/mg干重)相比,钙化,钙化明显减轻(均P＜0.01),但与对照组(4.11±2.23ug/mg干重)相比无显著性差异(P＞0.05)。病理组织学检查显示2,3-丁二醇组和1,3-丙二醇组试片仅有轻度形态学改变。而GA组和1,2-丙二醇组试片有严重的胶原变性,大片钙化。结论戊二醛鞣制的生物材料经2,3-丁二醇改性后,可显著减轻钙化。     

京尼平与戊二醛鞣制牛心包材料的对比研究

目的观察京尼平作为一种新型固定生物瓣膜组织交联剂的可行性。方法牛心包经脱细胞处理,分别用戊二醛和京尼平进行鞣制,光镜下观察脱细胞效果,电镜下观察胶原纤维排列;测试收缩温度;拉伸试验测定最大载荷;Wistar大鼠臀大肌皮下埋藏牛心包片,于4周及8周后取出,分别测试京尼平与戊二醛鞣制的牛心包的钙、磷含量。结果经脱细胞处理后,光镜下可见脱细胞处理的牛心包几乎没有细胞核,而未经脱细胞处理的牛心包可见弥漫存在的细胞核。电镜下可见京尼平鞣制的牛心包胶原纤维呈环状交联,戊二醛鞣制的牛心包胶原纤维呈网状交联,京尼平鞣制的牛心包胶原纤维排列较戊二醛鞣制的牛心包略为疏松,收缩温度略低于戊二醛鞣制的牛心包(P<0.05)。最大载荷无统计学差异(P>0.05)。但京尼平鞣制牛心包的钙、磷含量显著低于戊二醛鞣制的牛心包(P<0.05)。京尼平鞣制的牛心包8周的钙含量显著低于4周的钙含量(P<0.05)。结论京尼平鞣制的牛心包具有较高的强度和极低的钙、磷含量,且随时间延长京尼平鞣制的牛心包的钙含量有减少趋势,提示京尼平是一种很有前景的生物组织交联试剂。     

宫颈阴道分泌液中MMP-9、铜、钙、锌含量与胎膜早破关系的研究

目的研究胎膜早破(PROM)孕妇宫颈阴道分泌液中基质金属蛋白酶-9(MMP-9)、微量元素(铜、钙、锌)的含量与PROM的关系。方法选择临产前足月剖宫产分娩的胎膜早破者50例(PROM组)和正常未破膜者50例(对照组),分别用双抗体夹心ELISA法和溶出电极法测定血浆、阴道分泌液中MMP-9、铜、钙、锌的含量。结果PROM组血浆与阴道分泌液中MMP-9水平分别为3187.74±2107.60μg/L、301.61±182.42μg/L均明显高于对照组(1163.02±1131.41μg/L,177.11±106.90μg/L),差异有显著性(P<0.01);PROM组阴道分泌液中铜、钙、锌的含量分别为2.04±1.82μg/L、40.24±18.21μg/L、0.32±0.22μg/L,均明显低于对照组(3.66±2.10μg/L、49.30±12.78μg/L、0.69±0.41μg/L),差异有显著性(P<0.01)。结论胎膜早破孕妇阴道分泌液中MMP-9含量明显升高,而铜、钙、锌含量明显降低,对胎膜早破的早期诊断有一定帮助。     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies

Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.     

A CASE OF HUGE GASTRIC DISOPYROBEZOAR

PYTOBEZOARS are common bezoars in gastrontestinal tract, commonly seen in stomach and mall intestine,1but huge disopyrobezoars are     

Acupuncture Treatment of Chest Bi Syndrome

CASE HISTORY A male,a retired officer of 61 years old,paid his first visit on May 12,2006.The patient stated that 2 weeks before he suddenly got paroxysmal precordial choking pain,with irregular attacks.Each attack would last several seconds to several minutes.The attack was accompanied with heavy oppressed sensation in the precordial region,palpitation,lassitude,and in severe cases,sweating,which was once diagnosed by a hospital as'coronary heart disease'.Recalling the case history,the patient said that several days before he got angry with others because of some affairs in work,and he was not so used to the life pattern since he had just retired from his busy working post.At usual times,he was quick tempered and peevish.In recent days,he had poor appetite,and loose stool.     

Instructions to Contributors

The English edition of Chinese Journal of Integrative Medicine is edited and published by the Chinese Journal of Integrated Traditional and Western Medicine Press.Original scientific papers of advanced clinical and experimental medicine in the field of complementary/alternative medicine or integrative medicine are expected.     

Development Is A High Level Inheritance for China Time-honored Brand Products

<正>As culture is the soul of a nation,the three books,"History of Chinese Philosophy,""History of Chinese Culture"and"History of Chinese Science and Technology"have demonstrated the superiority of our great Chinese culture,and its significant impact in the field of international culture.Both core value and practical value of the Chinese culture are very clear,reflected in the views of universe,nature,life,health,disease,and therapy and related considerations,concepts,and technical methods.