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Objective:To investigate whether blood-brain barrier(BBB)served a key role in the edema-relief effect of bloodletting puncture at hand twelve Jing-well points(HTWP)in traumatic brain injury(TBI)and the potential molecular signaling pathways.Methods:Adult male Sprague-Dawley rats were assigned to the shamoperated(sham),TBI,and bloodletting puncture(bloodletting)groups(n=24 per group)using a randomized number table.The TBI model rats were induced by cortical contusion and then bloodletting puncture were performed at HTWP twice a day for 2 days.The neurological function and cerebral edema were evaluated by modified neurological severity score(mNSS),cerebral water content,magnetic resonance imaging and hematoxylin and eosin staining.Cerebral blood flow was measured by laser speckles.The protein levels of aquaporin 4(AQP4),matrix metalloproteinases 9(MMP9)and mitogen-activated protein kinase pathway(MAPK)signaling were detected by immunofluorescence staining and Western blot.Results:Compared with TBI group,bloodletting puncture improved neurological function at 24 and 48 h,alleviated cerebral edema at 48 h,and reduced the permeability of BBB induced by TBI(all P<0.05).The AQP4 and MMP9 which would disrupt the integrity of BBB were downregulated by bloodletting puncture(P<0.05 or P<0.01).In addition,the extracellular signal-regulated kinase(ERK)and p38 signaling pathways were inhibited by bloodletting puncture(P<0.05).Conclusions:Bloodletting puncture at HTWP might play a significant role in protecting BBB through regulating the expressions of MMP9 and AQP4 as well as corresponding regulatory upstream ERK and p38 signaling pathways.Therefore,bloodletting puncture at HTWP may be a promising therapeutic strategy for TBI-induced cerebral edema. 相似文献
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抗冻蛋白(antifreeze protein,AFP)是一类结构多样的蛋白质,具有热滞效应(thermal hysteresis,TH,降低冰点而不改变熔点)和重结晶抑制效应(recrystalization inhabition,RI).通过非共价吸附抑制机制吸附到冰核表面,限制冰晶生长和抑制冰晶重结晶,从而保护有机体免受结冰引起的伤害.由于抗冻蛋白具有阻止冰晶生长而不破坏细胞的特点,因而利用抗冻蛋白在低温中长期保存各种细胞、组织和器官,特别在器官移植中可能具有很好的应用前景. 相似文献
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Song Wu Feng Wan Yong-shun Gao Zhe Zhang Hong Zhao Zhong-qi Cui Ji-yan Xie 《中国医学科学杂志(英文版)》2014,29(4):208-213
Objective To assess clinical effectiveness of using bilateral pectoralis major or plus rectus abdominis muscle flaps in treating deep sternal wound infection (DSWI) following median sternotomy. Methods Between January 2009 and December 2013, 19 patients with DSWI after median sternotomy for cardiac surgery were admitted to our hospital, including 14 males (73.7%) and 5 females (26.3%), aged 55±13 (18-78) years. According to the Pairolero classification of infected median sternotomies, 3 (15.8%) patients were type II, and the other 16 (84.2%) were type III. Surgical procedure consisted of adequate debridement of infected sternum, costal cartilage, granulation, steel wires, suture residues and other foreign substances. Sternal reconstruction used the bilateral pectoralis major or plus rectus abdominis muscle flaps to obliterate dead space. The drainage tubes were placed and connected to a negative pressure generator for adequate drainage. Results There were no intraoperative deaths. In 15 patients (78.9%), bilateral pectoral muscle flaps were mobilized sufficiently to cover and stabilize the defect created by wound debridement. 4 patients (21.0%) needed bilateral pectoral muscle flaps plus rectus abdominis muscle flaps because their pectoralis major muscle flaps could not reach the lowest portion of the wound. 2 patients (10.5%) presented with subcutaneous infection, and 3 patients (15.8%) had hematoma. They recovered following local debridement and medication. 17 patients (89.5%) were examined at follow-up 12 months later, all healed and having stable sternum. No patients showed infection recurrence during the follow-up period over 12 months. Conclusion DSWI following median sternotomy may be effectively managed with adequate debridement of infected tissues and reconstruction with bilateral pectoralis major muscle or plus rectus abdominis muscle flap transposition. 相似文献
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病人,女,81岁.40年前在工作时双上肢不慎被火烧伤,当年在大连化工厂医院烧伤科给予取自体腹部皮肤行前臂植皮术,术后植皮区域皮肤成活修复良好.4年前无明显诱因双侧前臂植皮区域肤色逐渐变深,无意中发现皮肤明显松弛,无不适感.近3个月自觉植皮区域疼痛不适,局部无红肿等,轻微牵拉皮肤时疼痛明显.自发病起4年皮损未见扩大.患者既往体健,否认家族有类似病患者. 相似文献
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1 临床资料 患者,男,23岁,因左下肢疼痛20 d来医院就诊.询问病史:患者2 d前在上级医院诊断为坐骨神经痛.既往健康,无药物过敏史.门诊当日给予维生素B1 100 mg,维生素B12 250靏肌注,用药约5 min时患者感到头晕、胸闷、呼吸困难、寒战,面色苍白,脉搏105次·min-1,血压80/50 mmHg,考虑为药物所致过敏性休克,立即给予平卧,氧气吸入,肾上腺素1 mg肌注,地塞米松10 mg静注,5 min后患者恢复正常. 相似文献
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为确保消费的饮食安全,更好地了解饮食业餐具消毒效果卫生状况,从而为进一步加强监督管理提供依据,于2004年4~7月对邹城市所属中小型饮食业进行餐具消毒效果检测,共检测876份,现将结果报告如下。 相似文献
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目的 了解惠州市电子、五金、电镀等企业生产车间中有毒物污染情况,以便提出有效的预防措施。方法根据中华人民共和国国家标准中气相色谱法、原子吸收分光光度法、分光光度法对车间空气中有毒物质进行检测。结果2003~2004年共检测车间空气样品3625份,舍格3511份,总合格率为96.8%。其中锰尘舍格率最低,为82.2%;其次是三氯乙烯91.0%,氰化氢93.4%,甲苯97.1%,硫酸97.2%,铅烟98.2%,正己烷99.2%。苯99.3%,二甲苯99.6%,其他合格率为100%。结论惠州市部分电子、五金、电镀企业车间空气中有毒物的浓度较高,应改善生产车间的通风设施,做好工人的个人防护。 相似文献
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2005年汕头市登革热定点监测结果分析 总被引:5,自引:1,他引:5
目的 通过定点监测为汕头市登革热流行趋势的预测、预警和制定防治对策、措施提供科学依据。方法收集监测点的登革热疫情、人群抗体水平和媒介伊蚊等监测资料,用EPI2002软件统计分析。结果2005年汕头市无登革热疫情,抗登革热病毒IgG、IgM阳性率低,伊蚊幼虫孳生密度高峰在6、8、9、10月份,低谷在4、7、11月份,孳生情况随着月平均降水量、平均气温变化而变化;孳生场所以农村、暂时性容器为主。结论汕头市有登革热流行的自然、社会环境条件,人群普遍对登革热易感,8~10月是发动爱国卫生运动,清除伊蚊孳生地,预防、控制登革热流行的适当时机。 相似文献
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目的评价辖区内医疗机构消毒灭菌质量,督促各医疗单位提高消毒灭菌效果,防止医源性感染的发生。方法对辖区内的医疗单位已消毒或灭菌的用品进行抽样检测,将检测结果进行统计学分析。结果这次采集使用中消毒液、无菌器械、物体表面、手术室空气和医务人员手共3556份,检测达卫生标准2955份,符合率为83、10%,不同样品检测达卫生标准百分率依次为97.37%、95.56%、86.25%、75.41%和62、49%。结论高州市医疗机构消毒灭菌质量用卫生指标评价,卫生站的符合卫生标准率低于医院,手术室空气和医务人员手的监测合格率低于使用中消毒液和无菌器械。 相似文献
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目的分析湛江市2007年登革热监测结果,为登革热流行趋势的预测、预警和制定防治对策提供科学依据。方法收集监测点的登革热疫情、布雷图指数、人群抗体水平和媒介伊蚊等监测资料,用EPI2002软件统计分析。结果2007年湛江市暴发2起登革热疫情,发病205例,男97例,女108例;抗登革热病毒IgG阳性率低,伊蚊幼虫孳生密度高峰在9月份,低谷在6-8月份,阳性容器以永久性容器为主。结论湛江市有登革热流行的自然、社会环境条件,人群普遍对登革热易感,8-9月是发动爱国卫生运动,清除伊蚊孳生地,预防、控制登革热流行的适当时机。 相似文献
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为及时了解宁波市预防接种点的疫苗使用情况,科学地制定疫苗使用计划,我们于今年4-10月在镇海区开展常规接种疫苗(卡介苗BCG、脊灰三价糖丸TOPV、百白破三联疫苗DPT、麻疹减毒活疫苗MV)的损耗监测,现将监测结果评估如下。 相似文献
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羧甲基壳聚糖(Carboxymethyl Chitosan)是壳聚糖(Chitosan)经羧甲基化而得的一种水溶性多糖。与壳聚糖相比,羧甲基壳聚糖的水溶性提高,且安全、无毒、无害,具有成膜性、保湿性、生物相容性好的特点,是一种良好的药物载体。本文就对羧甲基壳聚糖在口腔医学中的应用做综述: 相似文献
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目的探讨ELISA检测法检测食品中氯霉素的可行性。方法采用国际上先进的ELISA检测法和酶标读数仪定量检测,并与高效液相色谱-电喷雾串联质谱法(HPLC—MS/MS)比较。结果氯霉素含量为0.05~0.20ug/kg之间,CV%为2.5%~5%,回收率为85.0%~110.0%,标准曲线r=-0.992~-0.999。结论ELISA法具有灵敏度高,干扰少,测定步骤简便、快速。操作安全,设备投资少,测定结果准确可靠。 相似文献