长波紫外线和中波紫外线对去卵巢骨质疏松大鼠1,25-二羟基维生素D3和骨代谢影响的比较

目的:探讨长波紫外线(ultraviolet A,UVA)和中波紫外线(ultraviolet B,UVB)对去卵巢骨质疏松(osteo-porosis)模型大鼠的血清1,25-二羟基维生素D3[1,25-dihydroxy-vitamin D3,1,25(OH)2D3]和骨代谢的影响,并比较UVA和UVB的治疗效果。方法:将40只健康雌性6月龄SD大鼠随机分为假手术对照组(sham)、模型组(OVX)、UVA照射组(OVX+UVA)、UVB照射组(OVX+UVB)。除假手术对照组外,其余各组经双侧卵巢切除术建立骨质疏松大鼠模型。模型建立后,对UVA照射组和UVB照射组分别使用波长340 nm和313 nm的紫外线进行照射治疗,15周后测定大鼠骨密度及血清1,25(OH)2D3、骨钙素(bone gamma-carboxyglutamic acid protein,BGP)、钙(calcium,Ca)和磷(phosphorus,P)的含量。结果:去卵巢术后6个月,与假手术对照组相比,模型组、UVA照射组和UVB照射组大鼠体重[(486.5±55.7)g,(488.3±32.1)g,(494.1±49.8)g,vs.(408.6±36.1)g,P<0.01]显著升高,股骨近心端骨密度[(0.318±0.025)g/cm2,(0.316±0.031)g/cm2,(0.322±0.036)g/cm2,vs.(0.386±0.027)g/cm2,P<0.01]、中心骨密度[(0.321±0.038)g/cm2,(0.319±0.051)g/cm2,(0.320±0.053)g/cm2,vs.(0.347±0.044)g/cm2,P<0.05)]和远心端骨密度[(0.320±0.028)g/cm2,(0.318±0.030)g/cm2,(0.322±0.036)g/cm2,vs.(0.361±0.046)g/cm2,P<0.01]显著降低,差异有统计学意义。紫外线照射15周后,与假手术对照组相比,模型组大鼠股骨近心端骨密度[(0.162±0.125)g/cm2vs.(0.293±0.076)g/cm2,P<0.01]、中心骨密度[(0.205±0.102)g/cm2vs.(0.306±0.031)g/cm2,P<0.01]和远心端骨密度[(0.153±0.119)g/cm2vs.(0.274±0.017)g/cm2,P<0.01]显著降低,血清1,25(OH)2D3[(19.80±1.67)ng/L vs.(28.35±4.32)ng/L,P<0.01]、骨钙素[(11.00±0.01)ng/L vs.(16.64±0.01)ng/L,P<0.01]和钙[(2.14±0.10)mmol/L vs.(2.68±0.16)mmol/L,P<0.01]含量显著降低,差异有统计学意义。与模型组相比,UVA照射组和UVB照射组股骨近心端骨密度[(0.248±0.092)g/cm2,(0.218±0.123)g/cm2,vs.(0.162±0.125)g/cm2,P<0.01]、中心骨密度[(0.272±0.010)g/cm2,(0.275±0.036)g/cm2,vs.(0.205±0.102)g/cm2,P<0.01]、远心端骨密度[(0.251±0.009)g/cm2,(0.242±0.063)g/cm2,vs.(0.153±0.119)g/cm2,P<0.01]显著升高,血清1,25(OH)2D3[(29.47±4.54)ng/L,(27.56±6.33)ng/L,vs.(19.80±1.67)ng/L,P<0.01]、骨钙素[(15.70±0.01)ng/L,(15.62±0.02)ng/L,vs.(11.00±0.01)ng/L,P<0.01]、钙[(2.48±0.22)mmol/L,(2.58±0.13)mmol/L,vs.(2.14±0.10)mmol/L,P<0.01]含量显著升高,差异有统计学意义。与UVB照射组相比,UVA照射组大鼠血清1,25(OH)2D3、骨钙素、钙和磷含量及骨密度的差异无统计学意义。结论:UVA和UVB均能提高去卵巢骨质疏松模型大鼠血清1,25(OH)2D3含量,促进骨形成,升高骨密度,缓解骨质疏松造成的骨质丢失。     

低强度脉冲电磁场对大鼠骨密度及骨形态计量学的影响

目的:探讨低强度脉冲电磁场对大鼠骨密度及生物力学特性的影响. 方法:将32只3 mo龄雌性SD大鼠随机分为4组(n=8):去势组、对照组、磁场A组、磁场B组;各组大鼠均采用下腹部切口,去势组及磁场组行双侧卵巢切除术,对照组仅行开腹术. 其中,磁场A组暴露于强度为4×10-4 T的脉冲磁场环境中,磁场B组暴露于强度为8×10-4 T的脉冲磁场环境中,两组磁场频率f均为15 Hz,τ为5 ms,暴磁时间为6 h/d. 各组动物饲养条件相同,8 wk后处死,测定全身骨密度及骨形态计量学指标. 结果:与去势组大鼠相比,磁场组全身骨密度、股骨远端骨密度、骨小梁面积百分比、骨小梁宽度、骨小梁数目均有增加(P<0.05),骨小梁间隙减小(P<0.05);而与对照组相比,磁场B组全身骨密度、骨小梁面积百分比以及骨小梁数目等指标间的差异则没有显著性意义(P>0.05);两磁场组之间作比较,全身骨密度和骨小梁数目的差异显著(P<0.05). 结论:PEMFs对卵巢切除大鼠骨质疏松症的发生有一定的预防和治疗作用,磁场生物效应存在明显的窗口效应.     

膜联蛋白5对雄性SD大鼠睾酮合成相关蛋白和酶表达的影响

目的 研究注射膜联蛋白5后雄性SD大鼠睾丸类固醇急性调节蛋白(Steroidogenic acute regulatory protein,StAR)、胆固醇侧链裂解酶(P450 cholesterol side-chain cleavage enzyme,P450scc)和3β-羟类固醇脱氢酶(3 beta-hydroxysteroid dehydrogenase,3β-HSD)表达的改变,初步探讨膜联蛋白5影响睾酮分泌的机制.方法 将20只SD大鼠随机分为对照组和实验组,实验组又分为7.5μg/kg组、15μg/kg组和30μg/kg组,每组5只.对照组大鼠腹腔注射等量的pH 8.0Tris-HCl,实验组腹腔注射不同剂量的膜联蛋白5,1次/d,连续20d.用RT-PCR方法分别检测对照组和各实验组SD大鼠睾丸组织中睾酮合成相关的StAR、P450scc和3β-HSD mRNA的表达变化,并用Western blot方法检测以上3种物质蛋白质水平的变化.结果 与对照组相比,在mRNA水平上,7.5μg/kg组和15μg/kg组大鼠睾丸组织P450scc mRNA表达分别增加了25.9%[(0.68±0.04) vs (0.54±0.03),P<0.01]和27.8%[(0.69±0.04) vs (0.54±0.03),P<0.01];3β-HSD mRNA表达分别升高了32.9%[(1.05±0.07) vs (0.79±0.10),P<0.01]和53.2%[(1.21±0.07) vs (0.79±0.10),P<0.01];而30μg/kg组表达差异无统计学意义;在蛋白水平上,15μg/kg组大鼠睾丸组织P450scc蛋白表达提高了34.2%[(0.37±0.04) vs (0.28±0.02),P<0.01],7.5μg/kg组和15μg/kg组的3β-HSD表达也分别增加了14.7%[(1.53±0.10) vs (1.34±0.05),P<0.01]和24.4%[(1.67±0.14) vs (1.34±0.05),P<0.01];而StAR的表达无论在转录水平还是在蛋白水平上,较对照组差异均无统计学意义.结论 膜联蛋白5可通过调节P450scc与3β-HSD的表达来调节睾酮合成.     

不同强度脉冲电磁场对大鼠骨质疏松的影响

目的:观察不同强度脉冲电磁场(PEMFs)对去势后大鼠骨质疏松症的影响.方法:30只雌性SD大鼠行双侧卵巢切除术建立骨质疏松动物模型,随机分为3组(n=10):磁场组暴露于强度分别为4×10-4 T,15×10-4 T的PEMFs环境中(f=15 Hz,τ=5 ms),暴磁时间为6 h/d;对照组饲养于线圈中,但不暴磁.12 wk后,对血清骨钙素、骨密度以及生物力学等参数进行检测.结果:与对照组相比,实验组骨密度、最大载荷及结构刚度均有增加(P＜0.05),血清骨钙素水平减少(P＜0.05);两个磁场组之间作比较,各指标的差异也具有统计学意义(P＜0.05).结论:PEMFs有益于骨质疏松症的预防和治疗,磁场参数的选择对其疗效有影响.     

电磁场对绝经后大鼠骨质疏松的影响

目的:观察电磁场(EMFs)对去势(绝经)后大鼠骨质疏松症的影响.方法:20只雌性SD大鼠行双侧卵巢切除术建立骨质疏松动物模型,随机分为两组(n=10): 实验组每日电磁场辐照6 h(8 ×10-4 T, 15 Hz),对照组饲养环境相同,但不暴磁. 12 wk后,对血清骨钙素、骨密度以及生物力学等参数进行检测.结果:与对照组相比,实验组骨密度、最大载荷及结构刚度均有增加(P＜0.05),血清骨钙素(BGP)水平减少(P＜0.05).结论:EMFs有益于骨质疏松症的预防和治疗.     

腰椎压缩性骨折患者骨密度水平与瘦素及瘦素受体的相关性分析

目的 探讨腰椎压缩性骨折患者骨密度水平与瘦素、瘦素受体的相关性。 方法 自2016年1月-2017年1月,连续性选择瑞安市人民医院收治的单一腰椎压缩性骨折患者50例作为观察组,同期收集50例健康成人作为对照组。检测骨折椎体、上下连接椎体骨密度、瘦素、可溶性瘦素受体,分析相应椎体与瘦素、可溶性瘦素受体的相关性。 结果 与对照组比较,观察组骨折椎体骨密度显著降低[(0.42±0.17) g/cm2 vs.(0.67±0.18) g/cm2,P<0.001];上一节段椎体骨密度显著降低[(0.48±0.12) g/cm2 vs. (0.61±0.15) g/cm2,P<0.001];下一节段椎体骨密度显著降低[(0.51±0.14) g/cm2 vs.(0.66±0.17)g/cm2,P<0.001];瘦素显著降低[(2.28±0.42)μg/ml vs. (2.74±0.71) μg/ml,P<0.001];可溶性瘦素受体显著增高[(25.57±7.48) kU/L vs. (21.62±8.12) kU/L,P=0.013)]。骨折椎体、上一节段、下一节段骨密度与血清瘦素均正相关(r=0.197、0.214和0.211,均P<0.05)。 结论 腰椎压缩性骨折患者血清瘦素显著降低,与椎体骨密度丢失有关。      

重组人甲状旁腺素(1-34)与降钙素治疗绝经后骨质疏松临床观察

目的:观察对比重组人甲状旁腺素(1-34)[Recombinant human parat-hyroid hormone,rhPTH(1-34)],后简称PTHI和降钙素(calcitionin,CT)对绝经后妇女骨质疏松症的疗效.方法:符合入选标准的56例绝经后妇女骨质疏松患者随机分为两组:PTH组(n=30)皮下注射重组人甲状旁腺素20 μg每天1次;CT组(n=26)肌肉注射降钙素20 Iu每周1次,两组均每日给予钙尔奇D 600mg/d,连续治疗6个月.比较治疗前后腰椎(L_(2～4))骨密度及T值,血钙磷及碱性磷酸酶(Alkaline phosphatase,AKP)等指标的变化及观察有无不良反应.结果:治疗后PTH组和CT组腰椎(L_(2～4))骨密度(Bone mineral density,BMD)均有明显增加[PTH组:(-4.09±1.30 vs-3.70±1.17)SD;CT组:(-4.03±1.27 vs-3.74±1.19)SD,均P<0.01].但两组间比较无明显差异.PTH组治疗后AKP明显增高[(88.2±28.2vs 123.2±34.2)U/L,P<0.05],而CT组无明显变化.另外,CT组治疗后血清胆固醇(Total cholesterol,TC)降低[(5.64±0.61 vs 5.22±0.70)mmol/L,P<0.O1].两组均无严重不良反应发生.结论:rhPTH(1-34)与降钙素都能显著提高BMD和缓解骨质疏松症状,对于治疗骨质疏松安全有效.     

贝伐单抗抑制碱烧伤角膜新生血管的实验研究

目的 评价结膜下注射贝伐单抗(bevacizumab)对大鼠碱烧伤角膜新生血管化的影响.方法 将22只成年雄性Wistar大鼠,碱烧伤诱导单眼角膜新生血管模型后随机分为两组:实验组和对照组.实验组(10眼)给予贝伐单抗原液(25me/ml)0.02 ml结膜下注射,对照组(10眼)给予结膜下注射等量生理盐水,两组给药时间均为碱烧伤第1天、第3天及第5天,每日1次,共3次.分别采用宏观测量新生血管长度、生长速度及新生血管化面积,显微镜下微血管计数方法研究角膜新生血管形成及抑制情况.结果 实验组新生血管生长速度较对照组慢[(1.05±0.62)mm/d vs(2.87±0.34)mm/d,P<0.05];新生血管化面积实验组较对照组小[(30.5±18.8)% vs (57.5±11)%,P<0.05];微血管计数实验组较对照组少,差异均具有统计学意义(1.86±1.54 vs 8.51±2.33,P<0.05).结论 贝伐单抗可抑制大鼠碱烧伤角膜新生血管的增殖.     

缺血后处理对缺血/再灌注损伤大鼠肾脏Fas,Bcl-2和Bax表达的影响

目的:探讨缺血后处理对缺血/再灌注(I/R)损伤大鼠肾脏Fas,Bax和Bcl-2表达的影响.方法:将24只SD大鼠随机分为假手术(S)组、I/R组和缺血后处理(IPO)组,每组8只,分别建立动物模型.各组分别采用免疫组化和免疫印迹法检测肾脏Fas,Bcl-2和Bax蛋白的表达变化.结果:IPO组较I/R组Fas阳性染色指数和表达显著降低,分别为[(31.17±4.81)% vs (36.26±5.32)%.P<0.05)]和[(0.98±0.11)vs(1.28±0.19),P<0.05)];IPO组和I/R组Bcl-2阳性染色指数和表达分别为[(32.34±5.12)%vs(31.33±4.55)%.P0.05)]和[(1.03±0.17)vs(0.75±0.11),P<0.05)],Bax阳性染色指数和表达分别为[(25.60±3.79)%vs(29.67±4.34)%,P<0.05)]和[(0.59±0.08)vs(0.91±0.16),P<0.05)].结论:IPO可使Fas和Bax的表达减少,同时可使Bcl-2的表达增加,从而减少缺血再灌注损伤大鼠肾脏细胞的凋亡.     

心房钠尿肽对急性肺损伤的治疗作用及其可能机制

目的:研究心房钠尿肽(ANP)对急性肺损伤时肺泡Ⅱ型上皮细胞(AT-Ⅱ)的保护作用及其机制. 方法:SD大鼠18只随机等分为生理盐水对照组、脂多糖(LPS)模型组、ANP治疗组. 通过颈外静脉给药,给药4 h后处死动物,收集肺泡灌洗液(BALF),测定BALF表面张力、总磷脂含量(TPL)、乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、丙二醛(MDA)及总蛋白(TP)含量. 结果:与对照组比较, LPS可使BALF中LDH活性[(4.8±1.9),(8.4±1.9) μkat/L]及TP含量升高(P<0.05),ANP[(5.1±1.7) μkat/L]可逆转此现象;LPS可使BALF中TPL[(0.43±0.04) vs (0.34±0.06) μg/kg]明显降低(P<0.05)、表面张力[(19.8±2.6) vs (23.3±2.9) mN/m]升高(P<0.05),ANP治疗后TPL[(0.41±0.06) μg/kg]升高、表面张力[(18.6±2.9) mN/m]降低;与对照组比较,LPS组AKP活性[(0.10±0.08) vs (0.36±0.10) μkat/L]升高(P<0.05),ANP治疗后AKP[(0.14±0.09) μkat/L]明显降低;ANP能逆转LPS引起的MDA含量升高. 结论: ANP能减轻LPS引起的急性肺损伤,其机制可能与ANP保护AT-Ⅱ并促进其分泌表面活性物质有关.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

The clinicopathological and immuohistochemical analysis of solid-pseudopapillary tumor of the pancreas： report of 9 cases

Objective：To investigate the clinical features, pathological characteristics and immunophenotype of solid-pseudopapillary tumor of the pancreas（SPTP）. Methods：Nine surgically treated cases of SPTP were retrospectively reviewed. Hematoxylin and Eosin（HE） staining and immunohistochemical staining were used to analyze all cases, and the general clinical data was collected. Results：Six patients were asymptomatic except for a palpable mass. Two patients complained of vague-epigastric pain. One patient appeared jaundice. The tumor was encapsulated and solid tissues alternately with cystic tissues. Histologically, the histological structure of solid portion was pseudopapillary with a fibrovascular core. Tumor cells were uniform and medium-sized which were arranged in sheets ets or nests or pseudopapillary patterns. Immunohistochemical studies demonstrated that SPTP proved positive in vimentin（9/9 cases）, AAT（9/9 cases）, NSE（9/9 cases）, ACT（7/9 cases）, CK20（2/9 cases）, CgA（1/9 cases）, S-100（3/gcases）, PR（4/gcases）, Syn（3/9 cases） and CD56（5/9cases）, negative in CEA and ER. Conclusion：SPTP is a tumor predominantly occurring in young women frequently without special symptoms. This tumor has various characteristical histological patterns with different immunophenotype.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.