三氧化二砷对人卵巢癌细胞株体外作用的研究

目的：探讨三氧化二砷（As2O3)对卵巢癌细胞株体外生长的影响。方法：以As2O3处理卵巢癌细胞株SKOV3，采用四甲基偶氮唑蓝（MTT）比色法，测定As2O3对SKOV3生长的抑制作用，并绘制剂量－效应曲线；通过透射电镜观察细胞超微结构的变化；应用流式细胞仪分析DNA含量和细胞周期并测定凋亡特征酶caspase-3的活性变化。结果：0．5－4．0μmol/L三氧化二砷明显抑制SKOV3细胞的增殖，并呈剂量和时间依赖性；透射电镜观察可看到较为典型的细胞凋亡的形态学改变；细胞核固缩，染色质凝集，呈新月状紧贴于核膜周边，凋亡小体形成等；流式细胞仪检测出现DNA含量小于G1峰的亚二倍体凋亡峰，同时出现G2／M期阻滞；SKOV3在As2O3作用下，caspase-3是实现凋亡的效应因子，它的激活必然导致凋亡细胞最终的各种表型变化。结论：As2O3对卵巢癌细胞株SKOV3的生长有明显的抑制作用，诱导凋亡是其主要作用机制之一。     

雄黄诱导卵巢癌细胞珠SKOV3和COC1凋亡的实验研究

目的:探讨雄黄对卵巢癌细胞株SKOV3和COC1增殖能力的影响及可能机理。方法:采用不同浓度雄黄溶液作用于两珠细胞,用MTT法检测生长抑制率;流式细胞仪检测细胞凋亡率及分析细胞周期;原位末端标记法(TUNEL)观察细胞凋亡形态学。结果:不同浓度的雄黄溶液对卵巢癌细胞株SKOV3及COC1均有不同程度的生长抑制作用,具有浓度和时间依赖性,且COC1比SKOV3对雄黄更敏感。结论:雄黄对卵巢癌细胞有生长抑制作用,诱导细胞发生G1期阻滞是雄黄抑制卵巢癌细胞生长作用的可能机制之一。     

As2O3对卵巢癌耐顺铂细胞珠的诱导凋亡作用

目的 研究三氧化二砷(As2O3)对卵巢癌耐顺铂细胞株COC1/DDP的作用,探讨As2O3抑制肿瘤细胞生长的作用机制.方法 ①用MTT法检测不同浓度、不同作用时间的As2O3 对COC1/DDP的生长抑制率.②用Annexin V-FITC/PI双染色流式细胞术检测As2O3作用于COC1/DDP发生的细胞凋亡.③用cDNA基因芯片筛查与As2O3诱导凋亡发生相关的基因,并用实时定量PCR技术验证结果.结果 ①As2O3对COC1/DDP有生长抑制作用,其生长抑制作用随着药物浓度的升高和作用时间的延长逐渐增强.②As2O3对COC1/DDP有凋亡诱导作用,随时间延长和剂量加大,凋亡发生率增加(P＜0.05).2.0 μmol/L As2O3作用24 h开始出现凋亡细胞,72 h达高峰,96 h细胞出现坏死. ③COC1/DDP细胞在As2O3作用前后上调基因15个,下调基因10个, 其中凋亡相关基因Bax、p53、c-Myc的上调,以及耐药基因LRP的下调与As2O3诱导COC1/DDP发生凋亡有关.基因芯片的结果与实时定量PCR验证结果相符合.结论 As2O3对卵巢癌耐药细胞株COC1/DDP有生长抑制作用,发生机制与As2O3诱导细胞凋亡发生有关.促凋亡基因和耐药相关基因参与As2O3诱导卵巢癌耐药细胞株凋亡的作用.     

As2O3对卵巢癌耐顺铂细胞珠的诱导凋亡作用

目的研究三氧化二砷(As2O3)对卵巢癌耐顺铂细胞株COC1/DDP的作用,探讨As2O3抑制肿瘤细胞生长的作用机制。方法①用MTT法检测不同浓度、不同作用时间的As2O3对COC1/DDP的生长抑制率。②用Annexin V-FITC/PI双染色流式细胞术检测As2O3作用于COC1/DDP发生的细胞凋亡。③用cDNA基因芯片筛查与As2O3诱导凋亡发生相关的基因,并用实时定量PCR技术验证结果。结果①As2O3对COC1/DDP有生长抑制作用,其生长抑制作用随着药物浓度的升高和作用时间的延长逐渐增强。②As2O3对COC1/DDP有凋亡诱导作用,随时间延长和剂量加大,凋亡发生率增加(P<0.05)。2.0μmol/LAs2O3作用24 h开始出现凋亡细胞,72 h达高峰,96 h细胞出现坏死。③COC1/DDP细胞在As2O3作用前后上调基因15个,下调基因10个,其中凋亡相关基因Bax、p53、c-Myc的上调,以及耐药基因LRP的下调与As2O3诱导COC1/DDP发生凋亡有关。基因芯片的结果与实时定量PCR验证结果相符合。结论As2O3对卵巢癌耐药细胞株COC1/DDP有生长抑制作用,发生机制与As2O3诱导细胞凋亡发生有关。促凋亡基因和耐药相关基因参与As2O3诱导卵巢癌耐药细胞株凋亡的作用。     

As2O3诱导人膀胱癌BIU-87细胞株凋亡及其对细胞周期的影响

目的:观察三氧化二砷(As2O3)对人膀胱癌BIU-87细胞株生长抑制作用,并探讨对细胞周期及凋亡的影响.方法:CellTiter 96 Aqueous One 试剂检测BIU-87细胞株增殖活力;荧光染色观察凋亡细胞的形态学变化;流式细胞术分析细胞周期及凋亡.结果:As2O3与BIU-87细胞株生长抑制率之间有明显的浓度依赖关系 (P<0.01),IC50为28.92 μmol/L;荧光显微镜下观察到经As2O3作用的BIU-87细胞核膜消失,核染色质呈大小不等的小圆形碎片;流式细胞术结果表明30 μmol/L的As2O3处理BIU-87细胞株48 h后,G0/G1期细胞比例下降,S期细胞比例增加,凋亡率为47.37%.结论:As2O3能显著抑制BIU-87细胞株增殖;使细胞阻滞在S期,并进一步诱导细胞凋亡.     

三氧化二砷诱导喉癌Hep-2细胞凋亡及其对细胞周期的影响

目的 观察三氧化二砷（AS2O3）对喉癌Hep-2细胞株诱导细胞凋亡的作用和对细胞周期的影响。方法 体外培养的Hep-2细胞与不同浓度的AS2O3作用24、48、72h，通过MTT还原法检测细胞的生长抑制率，用倒置相差显微镜、流式细胞仪、荧光显微镜研究细胞凋亡的形态学改变，检测细胞凋亡率并进行细胞周期分析。结果 As2O3可有效抑制Hep-2细胞的生长，呈时间和浓度依赖性特点。形态学观察发现，As2O3诱导Hep-2细胞凋亡。2μmol/L As2O3作用24h，S期细胞比例增高，72h后，S期细胞明显下降，细胞大量凋亡。AS2O3在低浓度时，阻滞细胞通过S期，高浓度时诱导S期细胞凋亡。结论 AS2O3可诱导Hep-2细胞的凋亡，与细胞周期阻滞有关。     

细胞分化剂增强三氧化二砷诱导肝癌细胞的凋亡效应

目的：探讨细胞分化剂（CDA－Ⅱ）在三氧化二砷（As2O3)诱导肝癌细胞凋亡效应中的作用。方法：应用CDA－Ⅱ和As2O3共同处理肝癌细胞株BEL－7402、HepG2，通过四唑蓝比色法检测细胞存活率；用活细胞荧光染色观测细胞凋亡及形态学变化、流式细胞术分析细胞周期变化及凋亡率。结果：CDA－Ⅱ毒性作用很低，但可以显增强As2O3对肝癌细胞生长的抑制作用，1．0g/L CDA-Ⅱ便可使As2O3对两株细胞的半数抑制浓度由5．0μmol/L降低至1．0μmol/L（P＜0．01）。形态学可观察到CDA－Ⅱ明显加强了As2O3诱导的细胞凋亡，且与剂量有关；流式细胞术分析，低质量浓度的CDA－Ⅱ（＜2．0g/L)细胞生长阻滞于G2期较对照组多，而随着剂量的增加则凋亡细胞和滞留于G1期的细胞增多，低剂量CDA－Ⅱ与As2O3共同处理肝癌细胞后，凋亡率明显高于单独用As2O3。结论：CDA－Ⅱ可增强As2O3诱导肝癌细胞的凋亡效应，两药具有协同作用。     

三氧化二砷和顺铂对人卵巢癌细胞株3AO体外杀伤效应的对比研究

目的 :比较As2 O3 和顺铂 (cDDP)对卵巢癌细胞株的生长抑制作用和凋亡诱导作用。方法 :采用MTT测定法和流式细胞分析法 (FCM) ,检测As2 O3 对体外培养的人卵巢癌细胞株 3AO细胞的生长抑制率和诱导凋亡率及对细胞周期的影响 ,并与cDDP进行比较。采用吖啶橙染色 ,观察As2 O3 作用后 ,凋亡细胞的形态学变化。结果 :As2 O3 可以有效地抑制 3AO细胞生长并诱导其凋亡 ,具有量 效性及时 效性 ,与cDDP相比 ,差异有统计学意义 (P <0 .0 1)。As2 O3 作用后 ,S期细胞通过受阻 ,形态学观察发现细胞呈典型的凋亡细胞特征。结论 :与cDDP相比 ,As2 O3 对卵巢癌细胞株 3AO细胞具有更有效的生长抑制作用及诱导凋亡作用 ,并使S期细胞阻滞。     

三氧化二砷对人大肠癌细胞生长的抑制作用

目的探讨不同浓度的三氧化二砷对人大肠癌细胞株CCL-187细胞生长抑制率的影响。方法大肠癌细胞株CCL-187分别与1·0,2·5μmol/LAs2O3共同孵育1~6d,电镜下观察细胞形态学变化,MTT法计算细胞生长抑制率。结果MTT法显示1·0,2·5μmol/LAs2O3均能抑制大肠癌CCL-187细胞的生长增殖,且具有时间依赖性。As2O3对CCL-187细胞增殖的抑制程度随As2O3浓度的加大而加强。透射电镜显示:1·0μmol/LAs2O3促进细胞分化,但2·5μmol/LAs2O3能诱导细胞凋亡。结论不同浓度的三氧化二砷对人大肠癌CCL-187细胞生长均可产生抑制作用,作用机制为1·0μmol/LAs2O3促进癌细胞分化,而2·5μmol/LAs2O3则诱导细胞凋亡。     

三氧化二砷、顺铂对人卵巢癌细胞联合作用研究

目的探讨三氧化二砷(As2O3)、顺铂(DDP)对人卵巢癌细胞HO-8910的联合作用及其相关机制。方法采用形态学方法研究As2O3、DDP对细胞增生的影响,用流式细胞术分析细胞周期,免疫细胞化学染色法检测p53基因表达。结果1.As2O3、DDP协同作用时,浓度分别在1.5μmol/L以上、2μmol/L以上时,有显著性差异;当三氧化二砷、顺铂浓度分别是12μmol/L、4μmol/L时,72h时癌细胞接近100%死亡。2.As2O3、DDP引起HO-8910细胞S期阻滞、p53基因表达增强。结论As2O3、DDP对人卵巢癌细胞HO-8910的生长有协同抑制作用;As2O3、DDP联合作用引起卵巢癌细胞凋亡的机制与S期阻滞、p53基因表达增强有关。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.