上调miR-125b对肝癌Huh-7细胞系增殖及顺铂诱导凋亡的作用研究

目的 研究miR-125b在肝癌发生发展中的作用机制,及其表达对于肝细胞癌化疗耐受性的影响.方法 采用qPCR检测肝癌患者癌组织、癌旁组织、Huh-7细胞系(对照组)及转染miR-125b mimic的Huh-7细胞系(转染组)中miR-125b表达情况.采用流式细胞仪及MTT法检测对照组及转染组细胞周期及增殖情况.Western blot检测Huh-7细胞系转染后Mcl-1蛋白表达.设3组:对照组为正常培养细胞,miRNAcontrol组转染miRNA control,miR-125b mimic组转染miR-125b mimic.Western blot检测Mcl-1特异性siRNA阻断Mcl-1蛋白表达后,细胞凋亡相关蛋白caspase3的表达情况.设3组:对照组细胞正常培养;顺铂组加入顺铂,终浓度50 μg/mL培养;转染+顺铂组转染Mcl-1-siRNA,48 h后加入顺铂,终浓度50 μg/mL.结果 肝癌组织miR-125b表达较癌旁组织明显减低,为癌旁组织的(46.24±8.53)％,P＜0.05.miR-125b mimic转染Huh-7细胞后,转染组细胞G1/S比例较对照组明显增高(P ＜0.05);Mcl-1蛋白表达较对照及miRNAcontrol组下调(P＜0.05);转染+顺铂组中的Caspase3蛋白表达较对照组及顺铂组明显增高(P＜0.05).结论 上调miR-125b的表达可以抑制肝癌细胞增殖,抑制靶蛋白Mcl-1表达,促进顺铂诱导凋亡,具有抑癌基因的作用.     

外泌体转运miR-324-5p对胃癌MGC-803细胞增殖及凋亡的影响

目的探讨外泌体转运微小RNA(miR)-324-5p对胃癌MGC-803细胞增殖及凋亡的影响。方法培养人胃癌细胞系MGC-803并提取外泌体,制备外泌体转运miR-324-5p模拟物(mimic)。细胞分为对照组(细胞不进行处理)、外泌体组(外泌体混悬液)和miR-324-5p mimic组(加入外泌体转运miR-324-5p mimic混悬液),制备移植瘤模型。检测各组摄取外泌体效率。MTT法和流式细胞术检测细胞增殖率和凋亡率,qRT-PCR和Western blotting法检测各组瘤体中miR-324-5p、细胞周期依赖性蛋白激酶抑制因子1A(p21)、细胞周期蛋白D1(CyclinD1)和微管相关蛋白1轻链3(LC3)mRNA及蛋白的表达。结果外泌体组和miR-324-5p mimic组MGC-803细胞摄取外泌体效率分别为60%和57%。与对照组和外泌体组比较,miR-324-5p mimic组MGC-803细胞增殖率降低,凋亡率增加(P＜0.05);裸鼠移植瘤质量和CyclinD1 mRNA及蛋白降低,抑瘤率、miR-324-5p、p21、LC3 mRNA及蛋白增加(P＜0.05)。结论外泌体转运miR-324-5p能抑制MGC-803细胞增殖和促进MGC-803细胞凋亡,其机制可能是增加移植瘤中p21和LC3水平,降低CyclinD1水平,从而提高抑瘤率,降低移植瘤质量。     

lncRNA KTN1-AS1调节miR-153-3p/NFAT5轴对非小细胞肺癌细胞增殖、迁移和侵袭的影响

目的 探讨长链非编码RNA Kinectin 1反义RNA 1(lncRNA KTN1-AS1)调控miR-153-3p/活化T细胞核因子5(NFAT5)轴对非小细胞肺癌(NSCLC)细胞增殖、迁移和侵袭的影响。方法 实时荧光定量PCR(qRT-PCR)、Western blot分别检测NSCLC组织、癌旁组织、人正常肺上皮细胞BEAS-2B及NSCLC细胞系A549、HCC827、H1299中KTN1-AS1、miR-153-3p表达及NFAT5蛋白表达;将A549细胞分为Ct组、si-NC组、si-KTN1-AS1组、mimic NC组、miR-153-3p mimic组、miR-153-3p mimic+pcDNA组、miR-153-3p mimic+pcDNA-NFAT5组,qRT-PCR检测细胞中KTN1-AS1、miR-153-3p表达;CCK-8法检测细胞增殖;平板克隆实验检测细胞克隆形成能力;划痕愈合实验检测细胞迁移;Transwell检测细胞侵袭;Western blot检测NFAT5、细胞周期蛋白D1(CyclinD1)、基质金属蛋白酶(MMP)-2、MMP-9蛋白...     

miR-181a靶向作用CARF基因抑制胰腺癌细胞凋亡

目的：探讨miR-181a与CARF基因是否存在靶向关系,并研究其在调节胰腺癌细胞增殖凋亡过程中的作用。方法：利用Real-time PCR检测胰腺癌组织细胞中miR-181a的表达水平,通过miR-181a拮抗剂下调胰腺癌细胞中miR-181a浓度,并用MTT检测其细胞增殖能力变化。建立包含CARF基因3’-UTR序列的PGL-3载体,结合双荧光素酶报告基因系统验证CARF基因是否存在miR-181a的作用靶点,然后通过激动剂及拮抗剂调节胰腺癌细胞中miR-181a浓度,用Western blot检测相应细胞CARF基因蛋白表达情况。结果：miR-181a在胰腺癌组织/细胞中的表达水平明显高于正常胰腺组织/细胞（P＜0.05）。转染miR-181a拮抗剂可显著下调胰腺癌细胞中miR-181a的表达,并使其增殖能力明显下降（P＜0.05）。双荧光素酶报告基因系统提示miR-181a与包含CARF基因3’-UTR序列的载体共转染,可明显下调荧光素酶活性,且下调水平与miR-181a剂量呈负相关（P＜0.05）;CARF蛋白表达情况和胰腺癌细胞中miR-181a水平呈显著负相关（P＜0.05）。结论：miR-181a在胰腺癌细胞中存在高表达,通过下调CARF靶基因促进细胞增殖而推动胰腺癌的发展。     

miR-630对三阴性乳腺癌细胞侵袭的影响及其机制研究

目的 研究miR-630在人体三阴性乳腺癌组织中的表达情况,探讨miR-630是否通过下调Sox4影响三阴性乳腺癌细胞系MDA-MB-231细胞的侵袭.方法 收集157例行乳腺癌根治术患者癌组织标本及癌旁正常乳腺组织,其中三阴性乳腺癌36例,通过实时荧光定量PCR(RT-PCR)检测各组(正常乳腺组、非三阴性乳腺癌组、三阴性乳腺癌组)组织中与乳腺癌相关的miR-630、miR-21、miR-195、miR-134、miR-200a、miR-381、miR-1228的表达情况;蛋白免疫印迹试验(Western blot)检测3组标本中细胞侵袭相关蛋白COL1 A1、COL1A5、MMP-2、MMP-9的表达情况;进一步在细胞实验中,通过转染miR-630mimic或空质粒后,再转染过表达Sox4的质粒或空质粒至离体培养的细胞中,Western blot检测细胞中COL1A1、COL1A5、MMP-2、MMP-9、Sox4的表达情况,划痕实验和Transwell法观察细胞迁移侵袭情况,利用荧光素酶实验验证Sox4是miR-630的靶基因.结果 相比于正常乳腺组,miR-630、miR-21在三阴性乳腺癌组织中的表达降低(P＜0.01);miR-195、miR-134、miR-200a、miR-381、miR-1228的表达在3组间差异无统计学意义(P＞0.05);相比于正常乳腺组及非三阴性乳腺癌组,三阴性乳腺癌组织中COL1A1、COL1A5、MMP-2、MMP-9、Sox4的表达增多(P＜0.05).在细胞实验中,相比于空质粒组,转染miR-630 mimic后,MDA-MB-231细胞中COL1 A1、COL1A5、MMP-2、MMP-9、Sox4的表达减少(P＜0.05),MDA-MB-231细胞的迁移侵袭能力减弱(P＜0.01);荧光素酶实验结果显示,miR-630能够显著降低Sox4-3’-UTR质粒的荧光素活性(P＜0.05);相比于过表达miR-630+空质粒组,转染过表达miR-630+过表达Sox4组,MDA-MB-231细胞中COL1A1、COL1A5、MMP-2和MMP-9表达增加(P＜0.05),细胞的迁移侵袭能力增强(P＜0.01).结论 在三阴性乳腺癌组织中,miR-630低表达;miR-630可以通过下调Sox4从而抑制MDA-MB-231的迁移和侵袭.     

hsa-miR-23a-3p靶向TGFβ2对急性淋巴细胞白血病CEM/C1细胞增殖、凋亡、侵袭及细胞骨架重组的影响

目的:探究hsa-miR-23a-3p靶向转化生长因子β2(TGFβ2)对急性淋巴细胞白血病细胞株CEM/C1增殖、凋亡、侵袭及细胞骨架重组的影响.方法:将CEM/C1细胞随机分为4组:Control组、mimic组、pcDNA-TGFβ2组和mimic+TGFβ2组,采用Li-pofectamine 2000分别转染mimic对照、miR-23a-3p mimic、TGFβ2过表达质粒及共转染miR-23a-3p mimic+TGFβ2过表达质粒.实时荧光定量PCR(RT-qPCR)法检测miR-23a-3p、TGFβ2表达;荧光素酶报告实验验证miR-23a-3p与TGFβ2的靶向关系;克隆形成实验检测细胞增殖;流式细胞仪检测细胞凋亡;Transwell检测细胞侵袭;微管形成实验检测细胞微管形成的结节数;Western blotting法检测 Ki67、Survivin、Caspase-3、Bax、Bcl-2、VEGF 蛋白表达.结果:与 Control 组相比,mimic 组miR-23a-3p表达、细胞凋亡率、Caspase-3蛋白表达量和Bax/Bcl-2比值升高,TGFβ2mRNA表达、克隆形成率、微管形成结节数及Ki67、Survivin、VEGF蛋白表达量降低,侵袭细胞数减少(均P＜0.05),pcDNA-TGFβ2组与mimic组各指标变化相反(均P＜0.05);与pcDNA-TGFβ2组比较,mimic+TGFβ2组克隆形成率、微管形成结节数和Ki67、Survivin、VEGF蛋白表达量降低,细胞凋亡率和Caspase-3蛋白表达量及Bax/Bcl-2比值升高,侵袭细胞数减少(均P＜0.05).荧光素酶报告实验证实miR-23a-3p和TGFβ2存在靶向关系.结论:hsa-miR-23a-3p通过靶向下调TGFβ2表达抑制人白血病细胞增殖、侵袭、细胞骨架重组,促进细胞凋亡.     

微小RNA-134-5p靶向作用于EGFR对卵巢癌SKOV3和A2780细胞生长的影响

目的 探讨微小RNA-134-5p(miR-134-5p)靶向作用于表皮生长因子受体(EGFR)基因对卵巢癌细胞生长的影响.方法 以卵巢癌细胞系SKOV3和A2780为研究对象,根据处理方法分为对照组(转染miR-NC)和实验组(转染miR-134-5p).采用qRT-PCR和Western blot检测EGFR基因及下游靶蛋白的表达量;流式细胞术检测细胞周期分布和细胞凋亡情况;四甲基偶氮唑蓝(MTT)法和集落形成实验检测卵巢癌细胞增殖能力.结果 实验组EGFR基因及下游靶蛋白表达显著下调,其中SKOV3细胞中EGFR mRNA下调至48％(P＜0.05),A2780细胞中EGFR mRNA下调至47％(P＜0.05).实验组细胞的细胞周期明显受到抑制(P＜0.05),miR-134-5p通过EGFR靶蛋白诱导细胞凋亡(P＜0.05).实验组细胞的增殖活性和集落形成能力明显受到抑制(P＜0.05).结论 miR-134-5p可通过靶向抑制EGFR基因,促进细胞周期停滞和细胞凋亡,降低卵巢癌细胞的增殖能力.     

miR-4295在鼻咽癌细胞增殖中的作用

目的探讨miR-4295在鼻咽癌细胞发生发展及增殖过程中的作用及相关的分子机制。方法通过脂质体转染miR-4295 mimic,inhibitor及其NC到鼻咽癌细胞株6-10B,CNE2。分别应用MTT实验、软琼脂集落形成实验检测miR-4295对鼻咽癌细胞增殖能力的影响;WB和QPCR方法检测miR-4295、细胞周期素D1(CyclinD1)、p21cip1、Rb、pRb在细胞中蛋白及mRNA的表达水平。通过Targetscan分析预测miR-4295与SOX9的3’UTR的结合情况,采用双荧光素酶报告实验检测miR-4295与SOX9的结合情况,同时采用WB检测细胞株中SOX9的表达。结果MTT、软琼脂集落形成实验均显示过表达miR-4295后鼻咽癌细胞增殖能力明显增强,细胞生长速率高于NC组,细胞集落形成个数多于NC组,差异均有统计学意义(P0.05);而miR-4295沉默后鼻咽癌细胞增殖能力明显降低,与NC-in组相比,差异有统计学意义(P0.05);WB及QPCR结果显示:过表达miR-4295后,细胞周期蛋白CyclinD1表达升高而细胞周期抑制因子p21cip1下调;通过Targetscan预测发现miR-4295靶向结合SOX9的3’UTR,荧光素酶活性检测结果发现miR-4295过表达后荧光素酶活性明显低于NC组及突变组,差异有统计学意义(P0.05)。结论上调miR-4295可通过抑制SOX9蛋白翻译从而促进鼻咽癌细胞增殖能力,并调节细胞周期相关蛋白,在鼻咽癌发生发展中扮演重要的角色。     

微小RNA-325-3p和叉头框蛋白M1在胃腺癌中的表达及靶向关系研究

目的:探究微小RNA-325-3p(miR-325-3p)与叉头框蛋白M1(FOXM1)在胃腺癌中的表达，分析miR-325-3p与FOXM1的靶向关系。方法:选择人胃腺癌MGC-803、SGC-7901细胞系，分别构建miR-325-3p mimic组、miR-325-3p inhibitor组，并设立空白对照组，应用Western blot法检测FOXM1蛋白的表达，应用双荧光素酶报告基因实验验证miR-325-3p与FOXM1的靶向关系。收集确诊为胃腺癌并行根治术的42例患者的肿瘤组织和距肿物边缘>2 cm的癌旁正常胃黏膜组织，应用实时荧光定量PCR法(qRT-PCR)检测miR-325-3p的表达，应用免疫组化染色(EnVision法)检测组织中FOXM1的表达。结果:胃癌MGC-803和SGC-7901细胞系miR-325-3p mimic组FOXM1蛋白表达明显低于空白对照组，miR-325-3p inhibitor组FOXM1蛋白表达明显高于空白对照组(均P<0.05)。双荧光素酶报告基因实验显示，miR-325-3p与FOXM1具有靶向关系。胃腺癌组织中mi...     

MicroRNA-134对宫颈癌细胞增殖和凋亡的影响

目的 探讨人宫颈癌组织中microRNA-134（miR-134）的表达及对宫颈癌细胞增殖和凋亡的影响。方法 PCR法检测宫颈癌组织中miR-134表达水平;在HeLa细胞内过表达miR-134,通过平板克隆形成试验检测细胞增殖,通过流式细胞仪检测细胞周期及细胞凋亡;PCR及western blotting检测miR-134潜在靶点CCND1表达变化。结果 宫颈癌组织中miR-134的表达水平较正常宫颈黏膜组织低（P?<0.05）;过表达miR-134能够抑制宫颈癌HeLa细胞增殖（P?<0.05）、阻滞细胞周期进展（P?<0.05）,并促进细胞凋亡（P?< 0.05）;过表达miR-134后抑制HeLa细胞中CCND1 mRNA及蛋白的表达水平（P?<0.05）。结论 宫颈癌组织中miR-134表达水平降低,过表达miR-134能下调CCND1的表达发挥抗癌细胞生长作用。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.