灵芝多糖对小鼠腹腔巨噬细胞蛋白激酶A活性的影响

为观察灵芝多糖（ＧＬＢ７）体外对小鼠腹腔巨噬细胞（ＭΦ）蛋白激酶Ａ（ＰＫＡ）活性的影响,采用反相离子对高效液相色谱法测定ＭΦ中ＲＫＡ活力。结果表明ＧＬＢ７（４０ｍｇ／Ｌ）能引起小鼠腹腔ＭΦ中ＰＫＡ活性明显升高,１０ｍｉｎ达峰值,３０ｍｉｎ恢复到基础水平。提示灵芝多糖的免疫增强作用与其增强ＭΦ中ＰＫＡ活性有关。     

灵芝多糖对小鼠巨噬细胞cAMP含量的影响

目的：研究灵芝多糖（ＧＬＢ_７） 对小鼠巨噬细胞（ＭФ） 内ｃＡＭＰ含量的影响,为其免疫增强作用机制提供依据。方法：采用细胞培养和竞争性蛋白结合分析法测定小鼠腹腔ＭФ中ｃＡＭＰ含量。结果：ＧＬＢ_７ 能剂量依赖性引起小鼠腹腔ＭФ中ｃＡＭＰ浓度快速升高,５ ｍｉｎ 达峰值,之后缓慢下降,至３０ ｍｉｎ 时基本恢复原来水平。结论：ＧＬＢ_７ 引起小鼠ＭФｃＡＭＰ浓度快速升高,可能与其增强小鼠免疫功能有关。     

灵芝多糖引起小鼠腹腔巨噬细胞膜超极化

用激光扫描共聚焦显微 镜（ Ｌ Ｓ Ｃ Ｍ） 技 术,动态 监测了 灵芝多 糖 Ｇ Ｌ Ｐ 均 一体组 分 Ｇ Ｌ Ｂ７ 对小 鼠腹腔巨噬细胞（ ＭΦ） 膜电位（ Ｍ Ｐ） 的影 响,结 果 Ｇ Ｌ Ｂ７ 能 引起 小 鼠腹 腔 ＭΦ 膜快 速超 极化, 最 低降 低幅 度为 ３９ ±９ ％ ,之后慢慢复极,但在１０ ｍｉｎ 内未恢复至原来水平。提示灵芝多糖引起小鼠腹腔 ＭΦ 膜超极化是其信 号跨膜转导的重要机制。     

沙棘蜂王浆口服液对辐射所致小鼠脾亚细胞脂类过氧化的影响

１材料与方法１．１实验药物沙棘蜂王浆口服液：自制;维生素Ｅ：大连水产制药厂。１．２实验动物的选择和处理选用８～９Ｗ龄、体重１８～２２ｇ的ＬＡＣＡ品系小鼠,随机分为Ａ（空白对照）、Ｂ（辐射损伤）、Ｃ（维生素Ｅ）、Ｄ（口服液）４组,每组１０只。给药剂量分别为Ｄ组口服液０．２ｍｌ／只／ｄ,Ｃ组ＶｉｔＦ５０ｍｇ／只／ｄ．Ａ和Ｂ组用０．２ｍｌ蒸馏水代替药物,连续灌胃给要７ｄ,第７ｄ,Ｂ、Ｃ和Ｄ组用６０Ｃｏγ射线一次性全身照射８．５Ｇｙ,剂量率为２．０９６Ｇｙ／ｍｉｎ,照后继续给药。照后第３ｄ将小鼠用脊椎脱…     

黄芪多糖、人参茎叶皂甙对手术病人细胞免疫功能的影响

手术病人术后２天和７天外周血单个核细胞（ＰＢＭＣ）的Ｔ淋巴细胞转化及白介素２（ＩＬ２）的生成均明显降低。５０～２５０μｇ／ｍｌ的黄芪多糖（ＡＰＳ）、０．１～１００μｇ／ｍｌ的人参茎叶皂甙（ＧＳＬ）在体外对术后７天ＰＢＭＣ的Ｔ淋巴细胞转化及ＩＬ２的生成均具有不同程度的升高作用，表明ＡＰＳ、ＧＳＬ对手术病人ＰＢＭＣ具有直接的刺激活性。     

泻心汤对正常和多种糖尿病模型动物的降血糖作用

观察泻心汤对正常、四氧嘧啶（ＡＬＸ）致糖尿病（ＤＭ）小鼠及地塞米松致胰岛素抵抗（ＩＲ）、糖耐量减退（ＩＧＴ）、模型大鼠的影响。结果表明,泻心肠能拮抗ＡＬＸ诱导的小鼠高血糖,明显降低正常小鼠ＡＬＸ－ＤＭ模型小鼠的空腹血糖（ＦＢＧ）,地塞米松致ＩＲ大鼠模型的ＩＧＴ,降低病鼠的ＦＢＧ及口服葡萄糖（ＯＧＴＴ）后２ｈ血糖（２ｈＢＧ）,提示泻心肠具有类似磺脲类药物和双胍类药物的降糖作用。     

猴菇多糖对胃癌癌前病变的逆转治疗研究

目的：为筛选治疗胃癌前病变及早期胃癌的有效药物,应用猴菇多糖施以胃癌癌前病变患者。方法：慢性萎缩性胃炎（ＣｈｒｏｎｉｃＡｔｒｏｐｈｙＧａｓｔｒｉｔｉｓ,ＣＡＧ）伴肠上皮化生（Ｉｎｔｅｓｔｉｎａｌｍｅｔａｐｌａｓｉａ,ＩＭ）２０例,ＣＡＧ伴异型增生（Ｄｙｓｐｌａｓｉａ,ＤＹＳ）患者２０例,疗程６个月后进行治疗前后形态学对比观察。并进行ＤＮＡ含量分析及ＢＧＣｅｌｌＬｙｍｐｈｏｓａｒｃｏｍｏ－２（Ｂｃｌ     

三七总皂甙对沙土鼠脑缺血再灌注损伤的作用

阻断蒙古沙土鼠双侧颈总动脉造成脑缺血３０ｍｉｎ，再灌注１５ｍｉｎ可降低脑组织中ＬＤＨ、ＳＯＤ含量，升高ＭＤＡ、ＴＸＢ２含量，降低６－Ｋｅｔｏ－ＰＧＦ（１α）／ＴＸＢ２比值。缺血前３０ｍｉｎ，ｉｐＰＮＳ２００ｍｇ、３００ｍｇ／ｋｇ，能升高被损伤脑组织中ＬＤＨ、ＳＯＤ含量，降低ＭＤＡＴＸＢ２含量，纠正６－ｋｅｔｏ－ＰＧＦ（１α）／ＴＸＢ２比值。提示ＰＮＳ可能通过清除氧自由基、抑制脂质过氧化和稳定神经细胞膜来保护脑组织缺血再灌注损伤。     

莱葛浸膏对麻醉开胸犬血流动力学的作用

麻醉开胸犬经十二指肠给药莱葛浸膏（ＬＧＥ）２．７ｇ／ｋｇ，５，４ｇ／ｋｇ，３０ｍｉｎ后可见ＳＢＰ、ＤＢＰ、ＭＡＰ、ＨＲ、ＬＶＳＰ，＋ｄｐ／ｄｔｍａｘ、ＬＶＷＩ和ＴＰＶＲ明显降低，约持续９０～１５０ｍｉｎ，而一ｄｐ／ｄｔｍａｘ，ＬＶＥＤＰ、ＣＩ、ＣＯ、ＳＶ、ＳＩ无明显改变。１．３５ｇ／ｋｇ对上述指标均无明显影响。提示ＬＧＥ具有肯定的降压作用，主要与其抑制心肌收缩力，扩张血管及减慢心率有关。     

灵芝多糖GL-B的抑瘤作用和机制研究

目的：探讨灵芝多糖ＧＬ－Ｂ的抗肿瘤作用及其机制。方法：采用体内体外实验相结合方法，ＭＴＴ法测肿瘤细胞增殖程度，流式细胞仪测细胞凋亡，同时采用生物法ＴＮＦα，ＥＬＩＳＡ测定ＩＴＦγ，并用逆转录聚合酶链反应方法检测ｍＲＮＡ表达。结果；（１）ＧＬ－Ｂ５０，１００，２００μｇ／ｍｌ显著抑制小鼠移植性肉瘤Ｓ１８０的生长；（２）ＧＬ－Ｂ直接加入人急性早幼粒白血病细胞体外培养不能抑制其生长，亦无诱导其凋亡的作用     

灵芝多糖对小鼠T细胞三磷酸肌醇和二酰基甘油体外作用的研究

 目的 研究灵芝多糖（GLB₇）对小鼠T细胞三磷酸肌醇（IP₃）和二酰基甘油（DAG）的作用,确定其对T细胞信号转导途径。方法 采用细胞培养、放射免疫分析、离子交换层析和薄层色谱法测定小鼠脾脏T细胞中IP₃和DAG的变化。结果 GLB₇引起小鼠T细胞中IP3和DAG浓度升高。IP₃的达峰时间为30 s,百日咳毒素（PTX）对此现象有抑制作用;DAG的合成出现两个峰,第一个峰迅速短暂,峰值位于30 s,第二个峰是持续时间较长的迟发峰。GLB₇对活化的T细胞中IP₃和DAG未产生明显影响。结论 IP₃/Ca²⁺和DAG/PKC两条信息途径均参与了灵芝多糖对T细胞的免疫调节。     

Immunomodulatory effect of Glossogyne tenuifolia in murine peritoneal macrophages and splenocytes

Glossogyne tenuifolia Cass., a medicinal plant native to Taiwan, is traditionally used as an anti-inflammatory remedy. Oleanolic acid and luteolin-7-glucoside have been previously identified as active components of Glossogyne tenuifolia in the murine macrophage-like cell line, RAW264.7. Current study investigates the effect and mechanism of the ethanol extract of Glossogyne tenuifolia (GT) and its major constituents on the release of inflammatory mediators in activated elicited murine peritoneal macrophages and splenocytes. Our results showed that GT (up to 0.15 mg/ml) inhibited the production of proinflammatory mediators, TNF-alpha, IL-1beta, IL-6, nitric oxide (NO) and prostaglandin E(2) (PGE(2)) in LPS-activated macrophages, and IFN-gamma in PHA-activated splenocytes. GT also inhibited LPS-activated murine iNOS and COX-2 promoter activities in transiently transfected RAW264.7 cells. The major constituents, oleanolic acid and luteolin-7-glucoside, as well as its aglycone, luteolin, inhibited the release of NO, PGE(2), TNF-alpha and IL-1beta in activated peritoneal macrophages. However, only luteolin-7-glucoside and luteolin were able to reduce IFN-gamma release in PHA-stimulated splenocytes. To further investigate the possible mechanisms that interfere with LPS- and PHA-signaling, this study focused on nuclear factor-kappaB activation signaling pathways. Our results demonstrate that GT (0.075-0.15 mg/ml) treatment reduces nuclear factor-kappaB (NF-kappaB) DNA binding activity, as demonstrated by electrophoretic mobility shift assay (EMSA). Collectively, the results suggest that GT inhibits proinflammatory mediator synthesis in activated murine peritoneal macrophages and splenocytes, in part through NF-kappaB-dependent pathways.     

大枣中性多糖对小鼠腹腔巨噬细胞电位的影响

目的：探讨大枣中性多糖（JDP－N）体外对小鼠腹腔巨噬细胞（Mφ）膜电位的影响。方法：采用激光扫描共聚焦显微镜（LSCM）测定小鼠腹腔巨噬细胞内膜电位。结果：大枣中性多糖作用后巨噬细胞内DiBAC4(3)荧光强度明显增强。结论：大枣中性多糖能引起小鼠腹腔巨噬细胞膜去极化。     

Acanthopanax senticosus inhibits nitric oxide production in murine macrophages in vitro and in vivo

Excess nitric oxide (NO) production has been implicated in inflammatory diseases. The present study investigated the inhibitory effect of the stem bark extract of Acanthopanax senticosus (A. senticosus) on NO production in murine macrophages in vitro and in vivo. In vitro exposure of RAW264.7 cells to 1, 10, 50, 100, 250, 500 and 1000 microg/mL of A. senticosus extract significantly suppressed NO production induced by lipopolysaccharide (LPS) and interferon gamma (IFN-gamma) in a dose-dependent manner. In vitro exposure of mouse resident peritoneal macrophages to 1, 10, 100 and 1000 microg/mL of A. senticosus extract significantly suppressed NO production induced by LPS and IFN-gamma in a dose-dependent manner. In vivo administration of A. senticosus extract (50, 100 and 200 mg/kg) to KM mice dose-dependently inhibited LPS and IFN-gamma induced production of NO in isolated mouse peritoneal macrophages ex vivo. Exposure to A. senticosus extract had no effect on cell viability and systemic toxicity. The results demonstrated that the stem bark extract of A. senticosus extract inhibits NO production in murine macrophages in vitro and in vivo.     

大枣中性多糖对小鼠腹腔巨噬细胞内pH值的影响

目的：探讨大枣中性多糖（JDP－N）体外对小鼠腹腔巨噬细胞（MΦ）内pH值的影响。方法。采用激光扫描共聚焦显微镜（LSCM）测定小鼠腹腔巨噬细胞内pH值。结果：大枣中性多糖作用后巨噬细胞内SNARF－1荧光强度明显增强。结论：大枣 中性多糖能引起小鼠腹腔巨噬细胞内pH值升高。     

Anti-inflammatory activities of constituents isolated from Phyllanthus polyphyllus

Four compounds, including one benzenoid, 4-O-methylgallic acid (1), together with three arylnaphthalide lignans, namely phyllamyricin C (2), justicidin B (3) and diphyllin (4) were isolated from the whole plants of Phyllanthus polyphyllus L. (Euphorbiaceae). This was the first isolation report of compounds 1-4 from this plant species. The in vitro inhibitory effects of these compounds were evaluated on the production of nitric oxide (NO) and cytokines (tumor necrosis factor (TNF)-alpha and interleukin (IL)-12), from LPS/IFN-gamma activated murine peritoneal macrophages. The results indicated that the 50% inhibition concentration (IC(50)) values of NO production from activated peritoneal macrophages by compounds 1-4 were 100, 25, 12.5 and 50 microM, respectively. In parallel, these dilutions were approximately inhibited in a similar manner to that observed for cytokines (TNF-alpha, and IL-12) production. On the other hand, at 100 microM concentration compounds 3 and 4 showed 50% inhibition of NO production from peritoneal macrophages that had been pre-activated with LPS/IFN-gamma for 24h, whereas compounds 1 and 2 inhibited only about 20 and 10%, respectively. These results support the use of this plant for the treatment of inflammatory diseases in oriental traditional medicine.     

The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide production

The in vitro effect of aqueous extract of Nigella sativa seeds on nitric oxide (NO) production by murine macrophages was studied. Murine peritoneal macrophages were pre-incubated with the extract and then activated with Escherichia coli lipopolysaccharride. NO production was measured after 24 hours by spectrophotometry. The plant extract caused a dose-dependent decrease in NO production. Dialyzed preparation of the extract did not affect NO production. However, the boiled fraction of the extract resulted in a dose-dependent inhibition of NO apparently comparable to that of the whole extract. These results indicate that the aqueous extract of N. sativa seeds exhibits an inhibitory effect on nitric oxide production by murine macrophages and the active component(s) is/are non-protein in nature. In view of the fact that nitric oxide is a pro-inflammatory mediator, this study validates the traditional use of the Nigella sativa seeds for the treatment of rheumatism.