灵芝多糖对小鼠T细胞三磷酸肌醇和二酰基甘油体外作用的研究

 目的 研究灵芝多糖（GLB₇）对小鼠T细胞三磷酸肌醇（IP₃）和二酰基甘油（DAG）的作用，确定其对T细胞信号转导途径。方法 采用细胞培养、放射免疫分析、离子交换层析和薄层色谱法测定小鼠脾脏T细胞中IP₃和DAG的变化。结果 GLB₇引起小鼠T细胞中IP3和DAG浓度升高。IP₃的达峰时间为30 s，百日咳毒素（PTX）对此现象有抑制作用；DAG的合成出现两个峰，第一个峰迅速短暂，峰值位于30 s，第二个峰是持续时间较长的迟发峰。GLB₇对活化的T细胞中IP₃和DAG未产生明显影响。结论 IP₃/Ca²⁺和DAG/PKC两条信息途径均参与了灵芝多糖对T细胞的免疫调节。

Effects of ganoderma lucidum pllysaccharides on inositol trisphosphate ani diacylgiyceroi in murine T cells

OBJECTIVE To investigate the effects of GLB₇ on inositol trisphosphate（IP_{3/sub>）and diacylglycerol(DAG) in murine T cell and to provide a scientific evidence for its immunomodulatory mechanism.METHOD Cell culture,radio-immunological asssy, anion-exchange columns and thin-layer chromatography were used.RESULT GLB7 increased the production of IP3 and DAG in mice.The peak of IP3 was approached at 30 s.Pertussis toxin(PTX) inhibited GLB7 from increasing the production of IP3.In the production of DAG,two peak were found,the first peak was rapid and transitory, and the second was delayed. GLB7 didnt influence the production of IP3 and DAG in active T cells.CONCLUSION The both paths of signal transduction of IP3/Ca²⁺ and DAG/PKC were involved in the immunomodulatory of GLB7 to T cells.}