长效局麻药对中性粒细胞呼吸爆发的抑制作用

目的观察几种长效局麻药对多形核中性粒细胞(PMN)释放氧自由基的抑制作用。方法选取近2周未用过任何药物的健康志愿者12人,抽取静脉血经分离得PMN悬液。将每人的血样分成6组:Ⅰ、Ⅱ组分别为单纯的反应混合物和参考样品,含有不同局麻药(浓度均为1×10-4)的Ⅲ~Ⅵ组为实验组。以fMLP或PMA激活反应,用细胞色素C减少法测定活化PMN产生的细胞外O2-含量的变化。Ⅰ,Ⅱ两组光密度之差作为对照值,各实验组吸光度与Ⅱ组吸光度之差为依赖于O2-的细胞色素C减少值,以反映PMN的O2-释放。结果未加局麻药的PMN悬液O2-的释放量为9.3±4.7nmol/106个细胞。而含有罗哌卡因、S(-)布比卡因、布比卡因和地卡因的PMN悬液O2-的释放量分别是对照值的77.5%、75.8%、88.3%和61.3%(P<0.01)。结论地卡因、罗哌卡因、布比卡因和S(-)布比卡因对活化的PMN氧自由基的释放有不同程度的抑制作用,不同局麻药之间的差异可能与药物的立体异构和脂溶性有关。     

红花黄色素对乳腺癌细胞增殖和迁移的抑制作用及其分子机制

目的 研究红花黄色素 (CY) 对乳腺癌细胞增殖、凋亡、迁移和侵袭的影响以及相关的分子机制.方法 采用CCK-8法检测不同浓度和时间红花黄色素对人乳腺癌细胞MDA-MB-231的抑制作用;采用流式细胞术检测不同浓度红花黄色素对人乳腺癌细胞MDA-MB-231的促凋亡作用;采用Transwell法检测不同浓度红花黄色素对人乳腺癌细胞MDA-MB-231迁移和侵袭的影响;在不同浓度的红花黄色素的干预后, 通过western blot技术检测凋亡相关蛋白Cleaved-Caspase-3和生存相关蛋白p-Akt以及转移相关蛋白MMP2的表达.结果 (1) 红花黄色素对乳腺癌细胞的抑制作用具有浓度依赖性和时间依赖性; (2) 不同浓度的红花黄色素干预能显著促进乳腺癌细胞的凋亡 (P<0.01) ; (3) 不同浓度的红花黄色素干预能显著抑制p-Akt的表达并同时促进Cleaved-Caspase-3的表达; (4) 不同浓度的红花黄色素能够显著抑制乳腺癌细胞的迁移和侵袭能力 (P<0.01) , 并且能够抑制迁移相关蛋白MMP2的表达.结论 红花黄色素能在体外通过激活凋亡通路而促进凋亡, 并能通过抑制MMP2来抑制乳腺癌细胞的转移.红花黄色素是一种潜在的治疗乳腺癌的药物.     

葶苈子黄酮对血小板激活因子的拮抗作用

目的研究葶苈子黄酮对血小板激活因子(PAF)诱导的家兔中性粒细胞(PMN)和洗涤家兔血小板(WRP)的聚集及黏附作用的影响。方法比浊法观察PAF诱导的PMN(或WRP)聚集;PAF诱导已着色PMN(或WRP)黏附,比色法测定此PMN(或WRP)破膜后溶液吸光度,表示其黏附活性。结果葶苈子黄酮可明显抑制PAF诱导的PMN和WRP聚集及黏附活性的升高(P<0.05)。结论葶苈子黄酮具有拮抗PAF的作用。     

异普复合静脉麻醉对IL-6,CD18,SOA的影响

目的 :研究异丙酚或 /和普鲁卡因对佛波酯 (PMA)刺激下离体中性粒细胞 (PMN)表面粘附分子CD18表达、超氧化物阴离子 (SOA)释放及内毒素 (LPS)刺激下全血中IL - 6生成的影响。方法 :提纯PMN并用磷酸盐缓冲液 (PBS)制成悬液 ,样本随机分为 5组 :空白组 (PMN PBS) ,损伤组 (PMN PBS PMA 10 0ng/ml) ,异丙酚组 (PMN PMA 10 0ng/ml propofol 4 μg/ml) ,普鲁卡因组 (PMN PMA10 0ng/ml procaine 15μg/ml)及异普复合组 (PMN PMA 10 0ng/ml 2 μg/mlpropofol 8μg/mlprocaine)。另设相应浓度药物组。流式细胞仪检测CD18表达 ,细胞色素C还原法测定SOA释放 ,放免法测定全血中IL - 6生成。结果 :异丙酚、普鲁卡因单独或联合应用均可抑制中性粒细胞表面粘附分子CD18表达及SOA释放 ,异丙酚抑制效应强于普鲁卡因。异普复合组效应与异丙酚临床浓度组接近 ,强于普鲁卡因临床浓度组。异丙酚促进全血中IL- 6的生成 ,普鲁卡因抑制全血中IL - 6的生成 ,异普复合组作用接近普鲁卡因组。结论 :异丙酚普鲁卡因复合应用对抑制中性粒细胞粘附功能 ,减轻组织损伤作用具有明显的互补性 ,这为两种麻醉药物在临床实践中复合应用的合理性给予基础实验支持     

超声法提取红花中红花黄色素的工艺研究

目的研究超声法提取红花药材中红花黄色素的最佳工艺。方法以单因素实验分析了提取溶剂浓度、提取时间及料液比3个因素对红花黄色素提取率的影响。在单因素实验基础上，通过正交实验考察超声法提取的最佳工艺。结果影响超声法提取红花黄色素的因素中只有乙醇浓度对红花黄色素提取率的影响有显著性。最佳提取工艺为以50％乙醇溶液为提取溶剂，料液比1：10，超声提取60min。结论该方法采用全物理过程，无任何污染，是提取红花中红花黄色素的有效途径。     

烧伤后PMN粘附对肺微血管内皮细胞[Ca2+]i的影响

严重烧伤后早期肺组织微血管内中性粒细胞的粘附贴壁能使肺血管通透性增加[1],而这种改变则依赖于内皮细胞胞内钙离子浓度([Ca2+]i)的增加[2].本实验观察烧伤血清及烧伤后中性粒细胞(PMN)对肺微血管内皮细胞(PMEC)[Ca2+]i的影响,并探讨PMN粘附及其粘附分子CD11b/CD18在该影响中的介导作用.     

白细胞介素-10对小鼠中性粒细胞吞噬和杀菌活性的影响

目的: 探讨白细胞介素-10(IL-10)对中性粒细胞(PMN)吞噬和杀菌活性的影响,以了解IL-10对非特异性免疫的作用机制。方法: 小鼠麻醉后从眼球取血,与不同浓度IL-10在37℃水浴中作用2 h,然后加FITC标记大肠埃希菌,置37℃水浴中15 min,溶血剂去除红细胞,流式细胞仪检测各IL-10浓度组PMN的吞噬率。小鼠麻醉后局部消毒,摘眼球,无菌方法取血。与不同浓度IL-10在37℃下作用2 h,然后加大肠埃希菌置37℃水浴1 h,同时设PMN杀菌0 min组、无IL-10的PMN杀菌对照组,各组样品作适当稀释后接种琼脂平板,最后观察各组菌落数,与对照组比较,计算IL-10对杀菌力影响。结果: PMN的吞噬功能在IL-10浓度为1 ng/ml组减低(15.87 ±14.25)%,但差异无统计学意义(P > 0.05)。而PMN的杀菌功能在IL-10浓度为0.1 ng/ml组降低(39.59 ±37.36)%,在IL-10浓度为1 ng/ml组则降低(42.28 ±22.24)%,差异均有统计学意义(P <0.05)。结论: IL-10对PMN的吞噬功能有轻度的抑制作用,对PMN的杀菌功能有明显的抑制作用,对非特异性免疫亦有抑制作用。     

BN52021对血小板活化因子引起的银屑病患者中性白细胞聚集反应的影响

据《中华医学杂志》1994年74卷第6期报道沈阳军区总医院皮肤科刘宝军等,采用聚集仪测定了血小板活化因子(PAF)拮抗剂BN52021对PAF引起银屑病患者嗜中性白细胞(PMN)聚集反应的影响。 结果表明,PAF对PMN有较强的聚集作用,并呈剂量依赖关系。银屑病患者PMN对低浓度PAF聚集反应明显高于正常人(P<0.05),而对高浓度PAF     

乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用

目的 研究乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用及其机制.方法 体外培养的人脐静脉内皮细胞(HUVECs)通过肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)孵育后活化,正常人血液分离的中性粒细胞经TNF-α孵育后激活,活化的HUVECs与激活的中性粒细胞共孵育建立中性粒细胞介导的内皮细胞损伤体外模型.共孵育体系中加入不同浓度的乌司他丁(100、500、1 500、3 000和5 000 U/ml),MTT法检测内皮细胞存活率,同时检测培养液中乳酸脱氢酶活性(LDH)、一氧化氮(NO)含量和弹性蛋白酶活性.中性粒细胞与TNF-α及不同浓度的乌司他丁共孵育后,流式细胞仪检测细胞内弹性蛋白酶活性.结果 激活的中性粒细胞与活化的HUVECs共孵育可以显著降低内皮细胞存活率,同时培养液中LDH活性显著升高、NO含量显著降低、弹性蛋白酶活性显著升高,乌司他丁可以剂量依赖性对抗上述变化.同时,乌司他丁可以剂量依赖性降低TNF-α激活的中性粒细胞内弹性蛋白酶的活性.结论 乌司他丁对中性粒细胞介导的内皮细胞损伤的抑制作用具有剂量依赖性,其机制与乌司他丁抑制中性粒细胞内及释放到细胞外的弹性蛋白酶的活性有关.     

山莨菪碱(654—2)对白细胞计数及趋化性的影响(简报)

为探讨山茛菪碱(654-2)的肾上腺糖皮质激素样作用,本文观察654-2对外周血白细胞(WBC)计数及中性粒细胞趋化性的影响并与氢化考的松作用进行比较。由大鼠尾巴取血,常规计数WBC,用琼脂糖板法测定中性粒细胞(PMN)趋化性。由兔动脉取血分离PMN(纯度>90％)。制成2.5×10~7/ml细胞悬液,分别与不同剂量654-2温育15min。以经Zymosan激活的大鼠血清作趋化因子进行测定。初步结果表明:①给大鼠肌肉注入654-2(4mg/kg体重,共两次,间隔一小时)后4～5小时,外周血WBC开始明显     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.