SLE病人血和尿β_2－mG水平及其临床意义

测定４０例ＳＬＥ病人（ＳＬＥ组）、４７例非ＳＬＥ风湿性疾病病人（非ＳＬＥ组）血和尿β_２－ｍＧ水平，ＳＬＥ组同时测定Ａｎｔｉ－Ｓｍｉｔｈａｎｔｉｂｏｄｙ（Ｓｍｉｔｈ）、Ａｎｔｉ－ｄｓ－ＤＮＡａｎｔｉｂｏｄｙ（ｄｓ－ＡＮＡ）、Ａｎｔｉ－ＡＮＡａｎｔｉｂｏｄｙ（ＡＮＡ）、尿蛋白及血ＢＵＮ、Ｃｒ，探讨血和尿β２－ｍＧ同它们的关系，ＳＬＥ组与非ＳＬＥ组血β２－ｍＧ水平无显著差异（Ｐ＞０．０５），ＳＬＥ组尿β_２－ｍＧ升高与非ＳＬＥ组相比有显著差异（Ｐ＜０．０１）。在ＳＬＥ组，血和尿β_２－ｍＧ均阳性者（双阳）之比率高于Ｓｍｉｔｈ、ｄｓ－ＤＮＡ阳性之比率；若与ＡＮＡ相比则无显著差异。ＳＬＥ组尿β_２－ｍＧ先于血ＢＵＮ、Ｃｒ升高；在判定肾脏功能损伤程度上，其意义与尿蛋白相当。     

Study on Lymphocyte Activation and Proliferation Induced by Anti-CD3 McAb

ＳｔｕｄｙｏｎＬｙｍｐｈｏｃｙｔｅＡｃｔｉｖａｔｉｏｎａｎｄＰｒｏｌｉｆｅｒａｔｉｏｎＩｎｄｕｃｅｄｂｙＡｎｔｉ－ＣＤ３ＭｃＡｂＬＩＭｉｎｇ（李鸣）；ＹＡＮＧＪｉｎｇ（杨敬）；ＳＨＥＮＧｕａｎ－ｘｉｎ（沈关心）（ＤｅｐａｒｔｍｅｎｔｏｆＩｍｍｕｎｏｌ...     

SLE病人血和尿β2—mG水平及其临床意义

测定４０例ＳＬＥ病人（ＳＬＥ组）、４７例非ＳＬＥ风湿性疾病病人（非ＳＬＥ组）血和尿β２－ｍＧ水平，ＳＬＥ组同时测定Ａｎｔｉ－Ｓｍｉｔｈ ａｎｔｉｂｏｄｙ（Ｓｍｉｔｈ）、Ａｎｔｉ－ｄｓ－ＤＮＡ ａｎｔｉｂｏｄｙ（ｄｓ－ＡＮＡ）、Ａｎｔｉ－ＡＮＡ ａｎｔｉｂｏｄｙ（ＡＮＡ）、尿蛋白及血ＢＵＮ、Ｃｒ，探讨血和尿β２－ｍＧ同它们的关系。ＳＬＥ组与非ＳＬＥ组血β２－ｍＧ水平无显著差异（Ｐ〉０．０５），ＳＬＥ     

Effects and Mechanism of Total Saponins ofPanax Notoginseng on Anti－Inflammation and Analgesia

ＥｆｆｅｃｔｓａｎｄＭｅｃｈａｎｉｓｍｏｆＴｏｔａｌＳａｐｏｎｉｎｓｏｆＰａｎａｘＮｏｔｏｇｉｎｓｅｎｇｏｎＡｎｔｉ－ＩｎｆｌａｍｍａｔｉｏｎａｎｄＡｎａｌｇｅｓｉａＷａｎｇＹｉ－ｌｉｎｇ（王一菱）；ＣｈｅｎＤｉ（陈迪）；ＷｕＪｉｎｇ－ｌａｎ（吴景兰...     

Experimental Study on Effects of Mailuotong Granule(脉络通冲剂) on Anti-thromboangiitis Obliterans

ＥｘｐｅｒｉｍｅｎｔａｌＳｔｕｄｙｏｎＥｆｆｅｃｔｓｏｆＭａｉｌｕｏｔｏｎｇＧｒａｎｕｌｅｏｎＡｎｔｉ－ｔｈｒｏｍｂｏａｎｇｉｉｔｉｓＯｂｌｉｔｅｒａｎｓ￥ＷＡＮＧＪｕｎ；ＷＡＮＧＹｕ－ｓｈｅｎｇ；ＦＵＭａｎ－ｈｕａ；ｅｔａｌ（ＨｅｎａｎＩｎｓｔｉｔ...     

Clinical Study of Poria-Polyporus Anti-Diarrhea Oral Liquor in Treating Infantile Rotavirus Diarrhea

Ｃｌｉｎｉｃａｌ　Ｓｔｕｄｙ　ｏｆ　Ｐｏｒｉａ－Ｐｏｌｙｐｏｒｕｓ　Ａｎｔｉ－Ｄｉａｒｒｈｅａ　Ｏｒａｌ　Ｌｉｑｕｏｒ　ｉｎ　Ｔｒｅａｔｉｎｇ　Ｉｎｆａｎｔｉｌｅ　Ｒｏｔａｖｉｒｕｓ　ＤｉａｒｒｈｅａＷＡＮＧＳｈｕ－ｓｈａｎ（王树山）,ＹＡＮＧＳｏｎ...     

The Anti-Endotoxin and Anti-Inflammatory Mediator Effects of Traditional Chinese Herbs: Experimental Researches

Ｔｈｅ　Ａｎｔｉ－Ｅｎｄｏｔｏｘｉｎ　ａｎｄ　Ａｎｔｉ－Ｉｎｆｌａｍｍａｔｏｒｙ　Ｍｅｄｉａｔｏｒ　Ｅｆｆｅｃｔｓ　ｏｆ　Ｔｒａｄｉｔｉｏｎａｌ　Ｃｈｉｎｅｓｅ　Ｈｅｒｂｓ：　Ｅｘｐｅｒｉｍｅｎｔａｌ　ＲｅｓｅａｒｃｈｅｓＷＡＮＧＪｉｎ－ｄａ（Ｉｎｓ...     

Clinical Study on Epidural Injected Lappaconitine for Post－operative Analgesia

ＣｌｉｎｉｃａｌＳｔｕｄｙｏｎＥｐｉｄｕｒａｌＩｎｊｅｃｔｅｄＬａｐｐａｃｏｎｉｔｉｎｅｆｏｒＰｏｓｔ－ｏｐｅｒａｔｉｖｅＡｎａｌｇｅｓｉａＣＨＥＮＭｉｎ－ｇｕａｎｇ（陈敏光）；ＷＡＮＧＱｉ－ｈａｏ（王其豪）；ＬＩＮＹｕ－ｂｉｎｇ（林玉冰）；ＣＨＥＮ...     

EXPRESSION OF TGF－β_1，PDGF AND IGF－1 mRNA IN LUNG OF BLEOMYCIN－A_5－INDUCED PULMONARY FIBROSIS IN RATS

ＥＸＰＲＥＳＳＩＯＮＯＦＴＧＦ－β１，ＰＤＧＦＡＮＤＩＧＦ－１ｍＲＮＡＩＮＬＵＮＧＯＦＢＬＥＯＭＹＣＩＮ－Ａ５－ＩＮＤＵＣＥＤＰＵＬＭＯＮＡＲＹＦＩＢＲＯＳＩＳＩＮＲＡＴＳＬｉＨａｉｃｈａｏ李海潮，ＨｅＢｉｎｇ何冰，ＱｕｅＣｈｅｎｇｌｉ阙呈立ａｎｄＷ...     

STUDY ON THE MECHANISM AND EFFECTS OF GLY－TYR－NH_2 AND GLY－TYR－LYS ON RAT LUTEAL CELLS IN VITRO

ＳＴＵＤＹＯＮＴＨＥＭＥＣＨＡＮＩＳＭＡＮＤＥＦＦＥＣＴＳＯＦＧＬＹ－ＴＹＲ－ＮＨ_２ＡＮＤＧＬＹ－ＴＹＲ－ＬＹＳＯＮＲＡＴＬＵＴＥＡＬＣＥＬＬＳＩＮＶＩＴＲＯ￥ＷａｎｇＮａｉｇｏｎｇ（王乃功），ＧｕａｎＭｕｚｈｅｎ（关慕贞）ＷａｎｇＤｅｘｉｎ（王德?..     

CD3AK细胞的诱导及其体外抗肿瘤活性的实验研究

本研究采用抗ＣＤ３单克隆抗体辅以少量的基因重组人白细胞介素２和植物血凝素诱导外周血淋巴细胞，成功地研制出具有抗瘤活性的ＣＤ３ＭｃＡｂ激活的杀伤细胞。     

Anti-proliferative effects induced by anti-CD4 human/murine chimeric antibody and murine anti-CD4 monoclonal antibody

Summary The effects of chimeric anti-CD4 human/murine chimeric antibody and murine anti-CD4 monoclonal antibody (McAb) on the proliferation induced by anti-CD3 McAb, phytohemagglutinin (PHA), IL-2, and allogeneic cells were studied. The results showed that chimeric anti-CD4 antibody and murine anti-CD4 McAb could inhibit the proliferation induced by the above inducers and the inhibitory effects were related to the dosage of the antibodies. This project was supported by a grant from the National Sciences Foundation of China (Serial No. 39370628).     

α－CD_3单克隆抗体对肿瘤特异性T细胞的扩增和杀瘤活性的影响

为寻找更有效的肿瘤过继性免疫治疗的方案，用α－ＣＤ３单克隆抗体（单抗）激活ＦＢＬ－３红白血病肿瘤细胞免疫的小鼠脾细胞获取肿瘤特异性Ｔ细胞，并观察α－ＣＤ３单抗对肿瘤特异性Ｔ细胞的增殖、杀瘤活性及表型的影响。体外实验结果显示，免疫小鼠脾细胞经α－ＣＤ３单抗激活后，采用低剂量ＩＬ－２即可维持其长期高度的、持续的增殖；又培养过程中α－ＣＤ３单抗持续存在可大大促进其对肿瘤的特异性杀伤。细胞分型分析结果也支持了α－ＣＤ３单抗的持续存在对维持肿瘤特异性Ｔ细胞的抗瘤活性具有很重要的意义，是肿瘤过继免疫疗法中颇有前景的治疗方案。     

LC-CW对人LAK细胞的影响及其机理的研究

提取干酪乳酸杆菌细胞壁成分(LC-CW),研究其对人外周血LAK细胞的影响及其作用机理。结果表明,1.LC-CW可以单独或与IL-2协同,促进PBL的增殖。2.LC-CW可以增强rIL-2诱导的人外周血LAK细胞杀伤Raji细胞的作用,统计学分析有显著意义。但是单独使用LC-CW不能诱导LAK细胞杀伤活性。3.LC-CW可使PBL的CD_4~+,CD_(16)~+抗原表达增加,与IL-2协同,可增加CD_3~+、CD_4~+、CD_8~+和CD_(25)~+抗原的表达。4.LC-CW可促进PHA-P诱导的IL-2的产生。本研究结果提示LC-CW是一种重要的LAK细胞活性增强因子。     

Activation of systemic immune effectors and induction of cytokine production by direct intratumoral injection of IL-2 DNA liposome

ourpreviousstudiesreportedaprotocolwhichreliedonthedirecttransmissionoflL-2geneintoB16F1Otumorsinvivotogeneticallymodifythetumorsastheygrewinsitu.WhenIL-2DNAliposomewasdirectlyinjectedintothetumormassintratumorallyinthemurinemodels,adra-maticinhibitionoftumorgrowthwasobserved-Thesurvivaltimeofthetumor-bearingmicewereprolongedsignificantly.Byanalyzingthefunctionofthetumorcellsand'tumorinfiltratinglympho-cytes(TIL),G418-resistanttumorcellscouldbeseparatedfromthetumormassandhighlevelofIL-2c…     

体外诱导C57BL/6小鼠CIK细胞研究

目的:诱导培养C57BL/6小鼠CIK细胞,并检测其对肿瘤细胞杀伤作用。方法:使用三种细胞因子及抗CD3单抗体外诱导C57BL/6小鼠脾细胞,用CCK-8试剂检测对肿瘤细胞杀伤作用。结果:培养出的CIK细胞主要为CD8+NK1.1+细胞,对肿瘤细胞有较强杀伤作用。结论:成功诱导出C57BL/6小鼠CIK细胞。     

PHA胸导管来源诱生的LAK细胞的作用

利用胸导管引流治疗哮喘病的机会,研究了PHA在rIL-2诱导胸导管淋巴细胞成为LAK细胞中的作用。结果表明:PHA和rIL-2共同诱导TDL2周,细胞数量扩增约34倍。同时明显促进了DL的~3H-TdR和~3H-Leucine的参入,较对照组有明显差异(P<0.01)。其中rIL-2 100～1000U/ml和PHA组作用最佳,但PHA和rIL-2的协同作用持续时间短,第6d即开始下降,而且PHA参与诱导的TDL-LAK细胞的杀伤活性有所下降。     

Anti-Proliferative Effects Induced by Anti-CD4 Human/Murine Chimeric Antibody and Murine Anti-CD4 Monoclonal Antibody

ＣＤ４ｍｏｌｅｃｕｌｅｉｓａｓｐｅｃｉａｌｍａｒｋｅｒｏｆｔｈｅｈｅｌｐｅｒＴｌｙｍｐｈｏｃｙｔｅ（ＴＨ）ａｎｄｄｅｌａｙｅｄｔｙｐｅｈｙｐｅｒｓｅｎｓｉｔｉｖｉｔｙＴｌｙｍｐｈｏｃｙｔｅ（ＴＤＴＨ）ａｓｗｅｌａｓａｄｉｆｅｒｅｎｔｉａｔｉｏｎｍａｒ...     

Induction of specific immunosuppression of cardiac allograft rejection with monoclonal antibodies to CD44, LFA-1 and ICAM-1

In the p~ess of allograft rejechon, the interactionbetween multiple adhesitin molecules Promotes the development Of the inflallnnatory ~on that leads to aliceddestruchon. Ih both aamal and hUInan models of aliced~splantation, the expression of adheSion molecules, including letlkocyte funchon-associated antigen-l (on-l ),intel'Cellular adhesion molecule-l (ICal-l ), vascularcell adhesion molecule~l (VCAM~l) and CD44 over thesurfaCe of the endothelial cells has been shown tO increasedwi al…     

正常鼠和免疫鼠CD3AK细胞和LAK细胞杀伤活性的比较研究

实验取正常鼠和Ｐ８１５肿瘤细胞致敏的免疫鼠脾细胞，经抗ＣＤ３抗体与低剂量ＩＬ－２或高剂量ＩＬ－２分别诱生为ＣＤ３ＡＫ细胞和ＬＡＫ细胞，用改良的乳酸脱氢酶释放法以Ｐ８１５细胞和ＹＡＣ－１细胞为靶细胞，测定ＣＤ３ＡＫ和ＬＡＫ细胞的杀伤活性。结果表明：正常鼠ＣＤ３ＡＫ细胞对Ｐ８１５细胞的杀伤活性高于ＬＡＫ细胞，对ＹＡＣ－１细胞的杀伤活性低于ＬＡＫ细胞。免疫鼠ＬＡＫ细胞对Ｐ８１５细胞的杀伤活性明显增高，高