PHA胸导管来源诱生的LAK细胞的作用

利用胸导管引流治疗哮喘病的机会,研究了PHA在rIL-2诱导胸导管淋巴细胞成为LAK细胞中的作用。结果表明:PHA和rIL-2共同诱导TDL2周,细胞数量扩增约34倍。同时明显促进了DL的~3H-TdR和~3H-Leucine的参入,较对照组有明显差异(P<0.01)。其中rIL-2 100～1000U/ml和PHA组作用最佳,但PHA和rIL-2的协同作用持续时间短,第6d即开始下降,而且PHA参与诱导的TDL-LAK细胞的杀伤活性有所下降。

THE EFFECT OF PHA ON THE PROLIFERATION AND ANTI-TUMOR ACTIVITY OF LAK CELLS INDUCED BY rIL-2 FROM THORACIC DUCT LYMPHOCYTES

Thoracic duct lymphocytes (TDL) from patients with severe bronchial asthma receiving treatment of thoracic duct drainage (TDD) were used to study the effect of PHA on the proliferation and anti-tumor activity of TDL-LAK. The results showed that TDL proliferation expanded by 34-fold when cells were incubated with 1000 U/ml rIL-2 and 1% PHA for 14 days. The increased ~3H-TdR or ~3H-Leucine incorporation was found to be at the same time as the increase of the cells number, and was significantly different from the control (P<0. 01). However, the enhancing action of PHA and rIL-2 on TDL-LAK cells was not kept long and were halted at the 6 th day. It was also found that the anti-tumor activity of p-TDL-LAK induced by rIL-2 and PHA together was lower than that induced by rIL-2 alone.