Multiple Pharmacological Actions of Yiqi Huatan Decoction (益气化痰方) in A Model of Depression in Rats

Objective: To investigate the influence of Yiqi Huatan Decoction (益气化痰方, YHD) on a model of depression in rats under different pathological conditions. Methods: Thirty-two male SD rats were randomly divided into 4 groups of 8: normal, model, YHD, and maprotiline. The model group, YHD group and maprotiline group used separate feeding and rats were exposed to chronic and unpredictable stress to build the depression model. From day 2, the YHD group and maprotiline group were respectively given YHD (7 g/kg) and maprotiline (10 mg/kg) by gastrogavage once daily. The normal and model groups were given the same volume of drinking water. The medication duration were 21 days. At the end of the experiment, the serum levels of copper and zinc were determined by atomic absorption spectroscopy, plasma concentrations of adrenocorticotropic hormone (ACTH) and cortisol (COR) were detected by radioimmunoassay, and levels of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamine (5-HT) in the hypothalamus were analysed by high performance liquid chromatography-eletricochemistry. Results: Compared with the content of copper and zinc in the serum of rats in the normal group, serum copper levels in model rats were significantly increased and zinc content was significantly reduced (both P<0.05). Plasma concentrations of ACTH and COR in the model group were significantly increased compared with those in the normal group (P<0.05, P<0.01). The contents of NE, DA, and 5-HT in the hypothalamus of rats in the model group were significantly reduced compared with those of the normal group (P<0.05 or P<0.01). Compared with those in the model group, the serum copper content and plasma concentrations of ACTH and COR were significantly decreased (all P<0.05); meanwhile, serum zinc content and hypothalamic contents of NE, DA, and 5-HT were significantly increased in rats of the YHD group (all P<0.05). The same effects were also shown in the maprotiline group except for 5-HT (all P<0.05). Conclusion: The pharmacological actions of YHD for depression might be related to improving trace-element anomalies, reversing endocrine dysfunction, and modulating the disorders of monoaminergic neurotransmitters.     

Working memory function in Chinese dyslexic children: A near-infrared spectroscopy study

The deficiency theories of dyslexia are quite contradictory and the cross-cultural studies in recent years mainly focused on whether the dyslexics among cultures shared the same cognitive profile or just based on the language.This study used Near-Infrared Spectroscopy (NIRS) imaging to measure the regional cerebral blood volume (BV) and the changes of cerebral activation in the left prefrontal cortex of 12 Chinese dyslexic children and their 12 age-matched normal controls during the Paced Vis-ual Serial Addition Test (PVSAT).Results showed that the scores of PVSAT of dyslexic children were significantly lower than those of the normal children (t=3.33,P<0.01).The activations of the left pre-frontal cortex in the normal group were significantly greater than those of dyslexic children (all P<0.01).Our results indicated that Chinese dyslexia had a general deficiency in working memory and this may be caused by the abnormal metabolic activity of brain blood volume in the left prefrontal cortex and the deficits in brain function might be the basis of neuropathology of Chinese dyslexia.Present study sup-ports the difference on brain activation of dyslexics from different languages may be caused by the same cognitive system related to reading.     

Changes in ganglioside contents, plasma sialic acid and cAMP levels in experimental hepatoma in mice

Abstract The present sutdy was designed to assess whether changes in glycolipids and cyclic AMP contents might serve as markers for the diagnosis of malignancy in the liver. The experimental model was a transplantable murine hepatoma. Experimental mice were divided into three groups:(1) a therapeutic group. which had been transplanted with hepatoma and treated with the antimetabolism drug 5-flurouracil(0.2mg/day i.p).(2) a control group, which had been transplanted with hepatoma and treated with 0.2ml 0.9% NaCl day and (3) a normal group of mice . The ganglioside and cAMP contents in the hepatoma tissue, plasma cAMP, total and lipid-bound sialic acid levels and red blood cell memebrane sialic acid levels were determined. Results showed that the ganglioside content, total and lipid-bound sialic acid levels in the control group were significantly higher than those in the livers of normal mice(P<0.01) while these respective values in the therapeutic group were significantly lower than those in the control group(P<0.01).The cAMP levels of tumor tissues and plasma in the control group were lower than those in normal mice. No significant difference in red blood cell membrane sialic acid content was observed between the therapeutic and control groups though levels for both were higher than those in normal mice. These results indicate that ganglioside ocntent and sialic acid levels in hepatoma tissues were significantly elevated, and cAMP levels in hepatoma tissues were significantly decreased during proliferation and abnormal differentiation. Key words transplantable hepatoma, gangliosides , sialic acid, cyclic adenosine mono-phosphate, 5-flurouracil 摘自Molecular and cellular biochemistry, 2000; Vol 207,29～33 该杂志摘要被美国科学引证索引(SCI)收录     

环境烟草烟雾暴露对小鼠脑区神经递质表达的影响

目的 通过观察小鼠脑部神经递质的变化,初步探讨环境烟草烟雾(ETS)成分对小鼠神经系统的影响.方法 在染毒实验台内对小鼠染毒,染毒8周造模后进行小鼠脑区定位,免疫组织学方法和图像分析观察脑区神经递质表达的变化结果 (1)GABA在小鼠大脑皮层、海马部位的表达:ETS暴露组阳性细胞数量明显减少(大脑皮层:0.25±0.10;海马:0.19±0.07)(P＜0.05).(2)nAChR在大脑皮层的表达:ETS吸入组阳性表达明显增加(0.31±0.10)(P＜0.05).(3)NMDAR在小鼠大脑皮层、纹状体皮质的表达:ETS吸入组(0.31±0.08,0.35±0.11)、尼古丁(Nic)吸入组阳性表达增加明显(P＜0.05).结论 ETS、Nic可导致大脑皮层、下丘脑等部位神经递质及受体表达的变化.

Abstract：

Objective To explore the mechanism of the influence on mice brain of environmental tobacco smoke. Methods After the mice were placed into the bench for 8 weeks, the region of the mice brain was localized and the expression of neurotransmitters and neurotransmitters receptors were detected by immunohistochernistry.Results ( 1 ) The expression of GABA in the mice cerebral cortex ( CC ) ( 0. 25 ± 0. 06 ) and the hippocampus (Hip) (0. 19 ± 0. 07 ) were much higher in the ETS-exposed group than that in the control group(P＜ 0. 05 ). (2)The expression of nAChR on CC(0. 31 ±0. 10) was much more in the ETS-exposed group than that in control group(P＜0.05). (3) The expression of NMDAR( Glu receptors) on the CC and striate cortex were much higher in the ETS-exposed group(0.32 ±0. 10,0.38 ±0. 14), NIC-inhaling group(0.31 ±0. 08,0.31 ± 0. 11 ) than that in control group(P＜0. 05 ). Conclusions Long-term ETS-exposed and NiC-exposed environment could change the expression of neurotransmitter and its receptors.     

Changes of endothelin during cerebral vasospasm after experimental subarachnoid hemorrhage.

Laser-Doppler flowmetry was employed in the observation of regional cortical blood flow (rCBF) after experimental subarachnoid hemorrhage (SAH) in anesthetized rats and the contents of endothelin (ET) in the cerebral-spinal fluid (CSF), plasma, hypothalamus, cerebral cortex, cerebellum and medulla were simultaneously measured. There was a biphasic change of rCBF after SAH. The contents of ET in CSF, plasma and hypothalamus rose prominently in the early stage after SAH, and the ET-increase in CSF and hypothalamus was earlier than that in plasma. The changes of ET contents in CSF correlated well with that in hypothalamus. Our results suggest that ET probably is an early important factor which induces cerebral vasospasm (CVS) after SAH. The increase of ET in CSF, which may originate from the hypothalamus, might play a more important role in the development of CVS after SAH than that in plasma.     

Inhibitory effects of TNP-470 in combination with BCNU on tumor growth of human glioblastoma xenografts

This study investigated the effect of TNP-470 in combination with carmustine (BCNU) on the growth of subcutaneously implanted human glioblastoma xenografts in nude mice.Human glioblastoma U-251 cells (1×10 7) were injected into 24 nude mice subcutaneously.The tumor-bearing mice were randomly divided into 4 groups on the seventh day following tumor implantation:TNP-470 group,in which TNP-470 was given 30 mg/kg subcutaneously every other day 7 times;BCNU group,in which 20 mg/kg BCNU were injected into peritoneal cavity per 4 days 3 times;TNP-470 plus BCNU group,in which TNP-470 and BCNU were coadministered in the same manner as in the TNP-470 group and the BCNU group;control group,in which the mice were given 0.2 mL of the mixture including 3% ethanol,5% acacia and 0.9% saline subcutaneously every other day 7 times.The tumor size and weights were measured.The tumor microvessel density (MVD) was determined by immunostaining by using goat-anti-mouse polyclonal antibody CD105.The results showed that on the 21th day following treatment,the volume of xenografts in the TNP-470 plus BCNU group was (108.93±17.63)mm 3,markedly lower than that in the TNP-470 group [(576.10±114.29)mm 3 ] and the BCNU group [(473.01±48.04)mm 3 ] (both P<0.01).And the xenograft volume in these 3 treatment groups was even much lower than that in the control group [(1512.61±470.25) mm 3 ] (all P<0.01).There was no significant difference in the volume of xenografts between the TNP-470 group and the BCNU group (P>0.05).The inhibition rate of the tumor growth in the TNP-470 plus BCNU group was (92.80±11.37)%,notably higher than that in the TNP-470 group [(61.91±6.29)%] and the BCNU group [(68.73±9.65)%] (both P<0.01) on the 21th day following treatment.There was no significant difference in the inhibition rate of tumor growth between the TNP-470 group and the BCNU group (P>0.05).The MVD of xenografts in the TNP-470 plus BCNU group was decreased significantly as compared with that in the TNP-470 group or the BCNU group (both P     

PROPOFOL DECREASES 125Ⅰ-β-CIT BINDING TOTHE DOPAMINE TRANSPORTER

Objective To determine the changes of brain dopamine transporter in mice receiving propofol anesthesia, 125Ⅰ-β-CIT binding sites were observed at different time course. Methods 1. Twenty-seven normal Kunming mice were ran domizedly divided into 3 groups (n=9) and received intraperitoneal injection of propofol 100, 200 mg /kg and 10% intralipid (as control)respectively. The time of losing righting reflex and displaying excitatory symptoms were recorded within 10min after administration. 2. Sixty Kunming mice were randomizedly assigned into 2 groups ( n = 30). The mice were given 125Ⅰ-β-CIT in travenously and propofol 200mg/kg or 10% intralipid (as control) intraperitoneally. Five mice in every group were killed at dif ferent time course and their brain removed to isolate cerebellar, hypothalamus, striatum and cerebral cortex. After weighting brain tissues, the radioactivity of 125Ⅰ-β-CIT in different brain tissue was measured. Results 1. The time of losing righting reflex was reduced from 319. 167 + 88.228s in propofol 100mg/kg group to 231. 667 + 46. 233s in propofol 200mg/kg group, and it fell from 193.75 + 27. 233s to 145. 556 + 27. 437s for presenting excitatory activity. 2. Propofol intraperitoneal groups significantly decreased the combination of 125Ⅰ-β-CIT and dopamine transporter in the striatum (P<0.01) and cerebral cortex (P <0.05) 120min after injection of propofol compared with the control group. But propofol increased the binding (P<0.05) in the striatum 30min after injection. Conclusion The inhibitive effect of propofol on dopamine transporter to uptake dopamine in mice brain may contribute to some anesthetic mechanisms.     

Association between Cardiac Changes and Stress,and the Effect of Peroxisome Proliferator-activated Receptor-γ on Stress-induced Myocardial Injury in Mice

This study was aimed to investigate the effect of stress induced by high-intensity exercises on the cardiovascular system. In the epidemiological investigation, 200 subjects(test group) engaged in special high-intensity exercises, and 97 who lived and worked in the same environment and conditions as those in the test group, but did not participate in the exercises served as controls. In the second part of the study, 50 mice were randomly divided into control group, exhaustive swimming group, white noise group, exhaustive swimming plus white noise group, and pioglitazone intervention group. The results showed that the plasma concentrations of the myocardial injury markers heart fatty acid-binding protein(H-FABP), C-reactive protein(CRP), β-endorphin(β-EP) and levels of psychological stress were significantly increased in test group as compared with control group; special high-intensity exercises resulted in a significant elevation of the incidence of cardiac arrhythmias. Animal experiments showed that the plasma levels of corticosterone(CORT) and troponin I(Tn I) were raised while the level of SOD was reduced in exhaustive swimming group, white noise group, and exhaustive swimming plus white noise group. The expression levels of PPARγ m RNA and protein were decreased in myocardial tissues in these groups as well. HE staining showed no remarkable change in myocardial tissues in all the groups. Treatment with pioglitazone significantly decreased the plasma levels of Tn I and CORT, while increased the level of SOD and the expression levels of PPARγ m RNA and protein. It was concluded that the high-intensity exercises may induce a heavy physical and psychological stress and predispose the subjects to accumulated fatigue and sleep deprivation; high-intensity exercises also increases the incidence of arrhythmias and myocardial injury. PPARγ may be involved in the physical and psychological changes induced by high-intensity exercises.     

白松片对大鼠慢性应激抑郁模型的抗抑郁实验研究

目的：探讨慢性应激抑郁模型大鼠神经生化、神经内分泌的变化及白松片对其的干预作用。方法：60只SD雄性大鼠随机分为正常对照组（NC组）、模型对照组（MC组）、氟西汀对照组（FC组）和白松片治疗组（BST）,每组15只。采用长期轻度不可预见性应激＋孤养复制大鼠抑郁模型,用高效液相色谱仪法检测海马5-羟色胺（5-HT）、谷氨酸（Glu）、γ-氨基丁酸（GABA）含量;放射免疫方法测定大鼠血浆促肾上腺皮质素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质醇（CORT）的水平;应用逆转录聚合酶联反应方法测定大鼠下丘脑和大脑皮质CRH mRNA表达水平;免疫组织化学法测定海马脑源性神经营养因子、酪氨酸羟化酶的表达。结果：与NC比较,模型大鼠海马5-HT,GABA含量显著降低;血浆CRH,ACTH,CORT及海马Glu含量增加;脑内CRH mRNA表达显著升高（P〈0．01）;与MC组比,BST组和FC组大鼠海马5-HT,GABA含量升高（P〈0.01,或P〈0.05）;血浆CRH,ACTH,CORT及海马Glu含量降低;脑内CRH mRNA表达下降（P〈0．01）。结论：慢性轻度不可预见性应激可使大鼠神经内分泌、神经生化发生异常改变,白松片对此具有一定调节作用。     

健脾化湿通络法对佐剂性关节炎大鼠HPA轴功能的影响

目的观察佐剂性关节炎(adjuvant arthritis,AA)大鼠血清ACTH、CORT水平及下丘脑CRHmRNA、大脑皮质GRmRNA的表达,研究AA大鼠HPA轴的功能变化及中药干预机制。方法将84只Wistar雄性大鼠随机分为正常对照组、模型对照组、MTX组(甲氨蝶呤组)、TPT组(雷公藤多苷片组)、XFC低、中、高剂量组(新风胶囊低、中、高剂量组),每组12只。除正常对照组外,向每只大鼠右足跖皮内注射FCA0.1 mL致炎,复制佐剂性关节炎大鼠模型,致炎后第19天开始分组处理30d。采用ELISA法检测各组大鼠血清ACTH、CORT水平,RT-PCR法检测各组大鼠下丘脑CRHmR-NA、大脑皮质GRmRNA的表达。结果AA大鼠血清ACTH、CORT、大脑皮质GRmRNA表达显著升高(P0.01),下丘脑中CRHmRNA表达显著降低(P0.01);XFC治疗组能显著降低血清ACTH、CORT水平(P0.01),下调大脑皮质GRmRNA表达(P0.01),上调下丘脑CRHmRNA表达(P0.01)。结论AA大鼠存在CRH分泌调节受损或下丘脑水平生物合成不足,健脾化湿通络方药通过降低血清ACTH及CORT水平,下调大脑皮质GRmRNA表达,上调下丘脑CRHmRNA表达,改善AA大鼠HPA轴功能紊乱,起到抗炎抗免疫的作用。     

柴胡疏肝散对慢性应激抑郁模型大鼠行为及血浆促肾上腺皮质激素释放激素和促肾上腺皮质激素的影响

目的：探讨柴胡疏肝散对慢性应激抑郁模型大鼠行为学及血浆促肾上腺皮质激素释放激素（corticotropin releasing hormone,CRH）和促肾上腺皮质激素（adrenocorticotropic hormone,ACTH）浓度的影响。 方法：40只雄性SD大鼠随机分为空白对照组、模型组、氟西汀组及柴胡疏肝散组。采用慢性轻度不可预见性应激加孤养复制大鼠抑郁模型（4周）。所有大鼠在造模第15天开始灌胃,分别灌服等体积生理盐水（空白对照组、模型组）或氟西汀（1.8 mg/kg）及柴胡疏肝散（5.9 g/kg）药液,连续2周。选用敞箱实验得分和液体消耗量等指标评价各组大鼠行为学改变,采用放射免疫法检测各组血浆CRH和ACTH浓度。 结果：与空白对照组比,模型组大鼠体质量增加缓慢,敞箱实验中的水平运动、垂直运动得分、清洁动作次数显著减少,中央格停留时间显著延长,糖水消耗明显下降,纯水消耗显著增多,血浆CRH和ACTH浓度增高, 差异有统计学意义（P＜0.05）。柴胡疏肝散组大鼠抑郁样行为显著改善,血浆CRH和ACTH浓度较模型组显著降低,差异有统计学意义（P＜0.01）。 结论：慢性轻度不可预见性应激可使大鼠行为及神经内分泌发生异常改变,引起抑郁;柴胡疏肝散可调节慢性应激引起的HPA轴功能亢进,具有抗抑郁作用。     

抑郁症患者神经内分泌节律变化的对照研究

目的 探讨抑郁症患者下丘脑-垂体-肾上腺(HPA)轴、下丘脑-垂体-甲状腺(HPT)轴的功能和节律变化与临床症状晨重夕轻之间的关系.方法 采用放射免疫法对49例符合美国精神障碍诊断与统计手册第4版(DSM-Ⅳ)重性抑郁发作患者及38例对照组,对早7:00、晚7:00血浆皮质醇(CORT)、促肾上腺皮质激素(ACTH)、三碘甲状腺原胺酸(T3)、四碘甲状腺原胺酸(T4)、甲状腺刺激素(TSH)水平进行测定,采用多因素方差分析比较2组各项指标的变化、并与临床症状之间的关系进行分析.结果 病例组早晨血浆CORT、早晨、晚间TSH水平[分别为(365.94±120.78)nmol/L,(6.24±2.47)μIU/ml,(6.68±2.42)±IU/ml]较对照组[(284.91±83.39)nmol/L,(3.82±1.75)±IU/ml,(4.01±1.69)μIU/ml]明显增高,差异有显著性(P<0.05);病例组早晨、晚间血浆T4水平较对照组显著降低(P<0.05);血浆皮质醇水平的早晚变化在病例组与对照组之间差异有显著性(P<0.001);病例组血浆CORT、T3、T4、TSH水平的早晚变化在单相和双相抑郁障碍、伴有和不伴有精神病性症状的临床亚型之间均差异无显著性(均P>0.05).结论 抑郁症患者血浆皮质醇水平存在早晚异常变化,可能是抑郁症的一项特异的生物学标志.     

髓样细胞触发受体2在酪氨酸激酶结合蛋白基因敲除小鼠不同脑区的表达

目的比较髓样细胞触发受体2(triggering receptor expressed on myeloid cell 2,TREM2)在不同月龄酪氨酸激酶结合蛋白(tyrosine kinase binding protein,TYROBP)基因敲除小鼠和野生型小鼠不同脑区的表达差异,探讨TREM2、TYROBP与早发型阿尔茨海默病(early onset Alzheimer's disease,EOAD)的关系。方法健康TYROBP基因敲除的小鼠根据基因测序结果分成3组:纯合型(TYROBP-/-)组、杂合型(TYROBP-/+)组和野生型(wild type,WT)组,3组小鼠分别在2、4、6月龄各选10只,运用Western blot和RT-qPCR技术检测TREM2在前额叶、海马中的表达情况。结果(1)在前额叶中:Western blot和RT-qPCR共同显示,与各月龄WT组[2月龄:(0.993±0.048),(1.654±0.033);4月龄:(0.503±0.019),(2.169±0.023);6月龄:(0.600±0.036),(1.468±0.057)]相比,TREM2蛋白和mRNA在2月龄TYROBP-/+组[(0.746±0.062),(1.137±0.067)]和TYROBP-/-组[(0.661±0.028),(0.644±0.012)]的表达均降低,而在4月龄和6月龄TYROBP-/+组[4月龄:(1.140±0.006),(5.483±0.088);6月龄:(0.827±0.043),(3.020±0.082)]和TYROBP-/-组[4月龄:(1.071±0.010),(3.012±0.150);6月龄:(0.627±0.026),(1.633±0.027)]的表达均升高,差异具有统计学意义(F=12.946,134.445;725.318,289.202;12.172,202.791;均P<0.05),且4月龄小鼠升高更明显。(2)在海马中:Western blot显示,与各月龄WT组[2月龄:(1.268±0.036);4月龄:(0.813±0.010);6月龄:(0.312±0.021)]相比,TREM2蛋白在2月龄TYROBP-/+组[(0.804±0.034)]和TYROBP-/-组[(0.534±0.020)]的表达降低,而在4月龄和6月龄TYROBP-/+组[(0.932±0.011);(0.769±0.031)]和TYROBP-/-组[(0.910±0.014);(0.609±0.018)]的表达均升高,差异具有统计学意义(F=142.807,27.884,94.067;均P<0.05),且4月龄小鼠升高更明显。结论TREM2在2月龄TYROBP基因敲除小鼠脑组织中表达降低,在4月龄和6月龄TYROBP基因敲除小鼠脑组织中表达升高,推测TREM2/TYROBP信号通路参与EOAD的病理过程并且在EOAD的不同病理阶段发挥着不同的作用。     

急性脑缺血大鼠边缘系统谷氨酸及其受体对下丘脑—垂体—肾上腺 …

目的 观察脑缺血／再灌流过程中海马和下丘脑的谷氨酸,谷氨酸受体（ＧｌｕＲ）的变化特征及其对下丘脑－垂体－肾上腺轴（ＨＰＡ）活动的影响。方法 应用高效液相色谱,受体的放射配体结合分析,核酸原位杂交,放射免疫分析分别监测大脑中动脉闭塞海马和下丘脑的谷氨酸含量。促皮质释放激素（ＣＲＨ）信使核酸糖核糖（ｍＲＮＡ）表达水平,血浆促肾上腺皮质激素（ＡＣＴＨ）浓度变化。结果 动脉闭塞１５分钟,下丘脑,海马两部位     

应激抑郁模型大鼠脑内ERK1／2含量和活性的变化

目的探讨慢性轻度不可预见性应激抑郁模型大鼠脑内ERK1/2含量和活性的变化,为进一步从信号传导的角度阐明抑郁症的分子病理机制提供线索。方法20只SD2~3月龄雄性大鼠,随机分为正常对照组10只、应激抑郁模型组10只,应激抑郁模型组经过21d慢性轻度不可预见性应激,以旷场行为总分评定其应激前后行为学改变。正常对照组和抑郁模型组在应激后即断头处死,采用蛋白印迹技术检测大鼠额叶皮质、海马、下丘脑、纹状体磷酸化ERK1/2(p-ERK1/2)和非磷酸化ERK1/2的表达。结果经过21d的慢性应激,应激大鼠旷场行为总分较应激前显著减少(P<0.001)。应激后抑郁模型组额叶部位p-ERK1、p-ERK2含量较正常对照组低,差异有显著性(P<0.001),ERK1/2的表达两组间比较差异无显著性(P>0.05);海马部位p-ERK1、p-ERK2含量也较正常对照组低,但差异无显著性,海马部位ERK1/2的表达两组间比较差异无显著性(P>0.05);纹状体和下丘脑部位p-ERK1/2、ERK1/2含量在应激前后均无明显变化。结论应激大鼠额叶部位p-ERK1/2含量的降低提示ERK信号传导通路的下调可能参与了应激所致抑郁的机制。     

慢性应激对大鼠海马神经元PKA和P-CREB蛋白表达的影响及抗抑郁剂的拮抗作用

目的：探讨慢性轻度不可预见应激对大鼠海马神经元内环磷酸腺苷依赖性蛋白激酶A（cAMP—dependent protein kinaseA，PKA）和磷酸化的环磷酸腺苷反应元件结合蛋白（phosphorylated cAMP-responsive element binding protein，P—CREB）表达的影响以及抗抑郁剂（氟西汀）的拮抗作用。方法：将36只SD雄性大鼠随机均分为正常组、慢性应激模型组、氟西汀治疗组。选用慢性轻度不可预见性应激加孤养造模，应用免疫组织化学以及蛋白质印迹技术研究各组大鼠脑内PKA、磷酸化的CREB（P-CREB）在海马区域的分布以及表达量的差异。结果：免疫组织化学和蛋白免疫印迹结果显示模型组大鼠海马细胞内的PKA和P-CREB蛋白表达量明显低于正常组（P〈0．05）；氟西汀组大鼠海马细胞内PKA和P-CREB蛋白表达量明显高于模型组（P〈0．05）。结论：慢性轻度不可预见性应激可使大鼠海马神经元内的PKA和P—CREB蛋白表达水平下降，氟西汀具有一定的拮抗作用。     

柴胡疏肝散及其拆方对慢性应激抑郁大鼠模型海马组织ERK1/2 mRNA表达的影响

目的:探讨柴胡疏肝散及其拆方的抗抑郁作用及机制.方法:将SD大鼠随机分为正常对照组、模型对照组、全方干预组、拆方干预I组和II组、氟西汀对照组.采用慢性轻度不可预见性应激配合孤养复制抑郁模型;运用敞箱试验和蔗糖水消耗实验观察大鼠行为改变;荧光实时定量PCR(FQ-PCR)检测各组大鼠海马组织细胞外调节蛋白激酶(extr...     

舒郁散对慢性应激抑郁大鼠神经肽及海马神经元5-HT 表达的影响

目的观察中药舒郁散对慢性应激抑郁大鼠神经肽(neuropeptide Y,NPY)及海马5-HT表达的影响。方法 50只Wistar大鼠随机分为正常对照组、模型组、百忧解组、舒郁散小剂量组、舒郁散大剂量组;对大鼠进行21 d的应激刺激建立慢性应激抑郁大鼠模型,采用open-field方法观察各组处理前、后2 min内大鼠行走路线及格子交叉点数的变化,了解大鼠行为的改变,用竞争酶联免疫分析法测定各组大鼠血浆、海马NPY的含量变化,采用免疫组织化学染色,观察各组大鼠额叶皮质、海马5-HT的表达。结果模型组大鼠血浆、海马NPY含量明显下降,与对照组比较,P<0.05;舒郁散大剂量组、百忧解组治疗后大鼠血浆、海马NPY含量较模型组显著升高,与模型组比较,P<0.05。免疫组化结果显示:舒郁散、百忧解组大鼠额叶皮层、海马区5-HT免疫反应阳性神经元数目明显增加,平均光密度明显增加,与模型组比较差异有显著性。结论舒郁散能显著提高慢性应激抑郁大鼠血浆、海马NPY含量,明显增加大鼠额叶皮层、海马区5-HT免疫反应阳性神经元数目,上述作用与其剂量呈正相关。