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余焕玲 《中国儿童保健杂志》2021,29(4):349-351
胆固醇是大脑发育的重要物质基础,胚胎和胎儿发育需要母体持续转运胆固醇,氧化固醇是胆固醇的代谢物,包括胆固醇合成途径中的前体物和分结代谢过程中产生的代谢产物,可能与大脑功能密切相关。人群研究发现,孕妇血清/浆胆固醇水平与子代多动症、孤独症谱系障碍发病风险有关,但尚需进一步确证。 相似文献
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Objective To observe the neuro-protective effects of genistein (Gen) and folic acid (FA) on neurons membrane and mitochondrial membrane damaged by β-amyloid peptides 31-35 (Aβ31-35).Methods The primary cultured rat cerebral cortical neurons were randomly divided into DMEM(control) ,Aβ31-35 (25 μmol/L) ,Gen( Gen 27 μg/ml) ,FA( FA 40 μg/ml) and Gen + FA( Gen 27 μg/ml + FA 40 μg/ml).Gen and/or FA were added two hours before Aβ31-35 addition.After twenty four hours, MTT assay was performed to measure the viability of cultured neurons.Fluorescence polarization was performed to observe the neuron cell membrane fluidity.The mitochondrial membrane potential(MMP) was determined to investigate the alteration of mitochondrial structure and function of neurons by laser scanning confocal microscope and a flow cytometer was used to investigate the activation of mitochondrial permeability transition pore (MPTP).Each experiment was repeated three times.Results Compared with group Aβ31-35 (0.845 ± 0.050, F = 4.931, P < 0.05 =, the absorbance was significantly higher in group Gen (0.982 ± 0.110, t=3.523,P<0.01=,FA (0.947 ±0.061,t=2.745,P<0.01= and Gen+ FA (0.996 ± 0.090, t = 3.966, P < 0.01 =.The viscosity of cell neuron membrane in group Gen ( 1.75 ± 0.28,t=2.085,P<0.05=,FA (1.66±0.37,t=2.357,P<0.05= andGen + FA (1.50±0.20,t=3.784,P < 0.05 = was significantly lower than that in group Aβ31-35 (2.11 ± 0.44, F = 5.529, P < 0.01 =, which indicated the cell membrane fluidity was significantly higher in group Gen and/or FA than that in group Aβ31-35.MMP was significantly decreased by Aβ31-35 (3.364 ± 1.140, t= 3.949, P< 0.01 = when comparing to control group (6.383 ± 1.683) ,while it was significantly increased by Gen (5.286 ± 1.792,t=2.406,P<0.05=,FA (5.884±2.022,t=2.887,P<0.01= and Gen + FA (6.120 ±2.124,t=3.304,P < 0.01 = when comparing to group Aβ31-35 ( F = 7.585, P < 0.01 =.MPTP was activated by Aβ31-35 and Gen and/or FA could reverse this progress.Conclusion Gen and/or FA could protect the neuronal and mitochondrial membrane from the impairment induced by Aβ31-35. 相似文献
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目的 探讨孕前和孕期高脂饲料喂养对新生大鼠肠道菌群的影响。方法 12只3周龄无特定病原体(Specific Pathogen Free, SPF)级雌性Sprague-dawley(SD)大鼠,适应性喂养一周后,按体重随机分为对照组与高脂组,孕前分别用基础饲料与高脂饲料喂养至12周龄,与同周龄SPF级雄性SD大鼠合笼,次日观察到阴道栓或阴道涂片中发现精子后,判定受孕。孕鼠单笼饲养,孕期的饲料与孕前相同。收集出生后第0.5天(postnatal day 0.5,PD 0.5)仔鼠,称量体重,采集肠道组织并称量质量,计算肠道系数;进行回肠和结肠组织病理和相关基因检测;提取肠道内容物的细菌总DNA,通过16S rRNA测序分析肠道菌群。采用t检验或Mann-Whitney U检验分析数据。结果 与对照组相比,高脂组PD 0.5仔鼠体重、肠道质量、肠道系数无显著改变(t=-0.682,P=0.499;t=0.269,P=0.790;t=0.674,P=0.506);肠道病理形态无改变;结肠闭合蛋白-1(Claudin-1)、溶菌酶(Lysozyme)、再生胰岛衍生蛋白3伽马(regenerat... 相似文献
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肥胖儿童血脂、载脂蛋白与心血管疾病的关系 总被引:1,自引:0,他引:1
目的分析肥胖儿童血脂状况,了解其与心血管疾病的关系。方法收集首都医科大学附属北京儿童医院营养门诊就诊的4~16岁的肥胖儿童153例。男109例,女44例;年龄4~16岁。用电阻抗法测量和计算其体脂肪率、体脂肪量、除脂肪量。用软皮尺测量其腰围和臀围,公式计算腰臀比值。用皮褶计测量其肩胛骨下、肱三头肌皮褶厚度,公式计算其肩胛骨下肱三头肌皮褶厚度比值。同时进行其血清胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、载脂蛋白AⅠ和载脂蛋白B(ApoB)水平检测。应用SPSS11.0软件进行统计学分析。结果与心血管疾病相关的指标:高TC发生率为3.3%,高水平LDL-C发生率为6.0%,高TG发生率为24.7%,低水平HDL-C发生率为31.3%。10岁以上肥胖儿童体质量比率、体脂肪率与HDL-C水平呈弱负相关(r=-0.202,-0.211)。结论肥胖儿童血脂和载脂蛋白水平可评估心血管疾病危险因素。 相似文献