广西南北两地人群肝组织黄曲霉毒素B1暴露的对比

目的:通过比较广西北回归线两侧的南宁地区和桂林地区人群肝组织黄曲霉毒素B1(Aflatoxin B1,AFB1)的暴露水平,探讨AFB1暴露与原发性肝细胞癌(Hepatocellular carcinoma,HCC)发生的关系.方法:应用免疫组化法检测南宁和桂林地区共165例HCC患者和95例正常肝组织中AFB1-DN...     

229例术后腹腔感染的分析

目的：探讨术后腹腔感染发生规律,提高对该类疾病的诊疗水平。方法：回顾性分析我院普通外科2013-01-2013-11术后并发腹腔感染和细菌培养阳性的229病例资料。结果：1229例患者检出共267株细菌;革兰氏阴性菌共180株（67.4%）,革兰氏阳性菌共75株（28.1%）,真菌共12株（4.5%）;38例为两种细菌混合感染。2不同细菌感染原因：大肠埃希菌116株,以肝叶切除/胆囊切除/胆道探查术（31.0%）、结直肠肿瘤切除术（20.7%）最常见;肠球菌共48株中以胰腺手术（33.3%）、肝叶切除/胆囊切除/胆道探查术（31.3%）最常发生;肺炎克雷伯菌共25株中以肝叶切除/胆囊切除/胆道探查术（28.0%）、肠切除/肠道造口还纳术（21.4%）发生最多。结论：腹部术后腹腔感染以革兰氏阴性杆菌感染最常见,不同手术后感染细菌谱存在差异,临床应根据手术方式、细菌培养结果选择治疗方案。     

有序Logistic回归模型对肝细胞癌病理分化相关因素的研究

目的:探讨原发性肝细胞癌病理分化的临床影响因素。方法:采用病例回顾分析方法对317例原发性肝细胞癌患者进行临床资料调查;通过多分类有序Logistic回归模型进行单因素与多因素的分析。结果:单因素分析显示,15个因素中,年龄、体重、吸烟、饮酒、术前AFP水平及肝硬化与肝癌细胞分化有关;多因素分析显示,只有肝硬化存在相关性(P<0.05),无肝硬化及轻度肝硬化患者与中重度肝硬化患者的病理分化相比,其OR值分别是3.13和3.40。结论:肝硬化是肝癌细胞分化的一个影响因素。     

肝组织石蜡切片8-OHdG免疫组化方法的研究

目的:探讨肝组织石蜡切片8-OHdG免疫组织化学(免疫组化)染色的实验方法.方法:以皮肤组织作为阳性对照,PBS代替一抗作为阴性对照,采用4种不同实验条件在肝组织石蜡切片中行8-OHdG免疫组化方法染色.结果:实验条件3,即高温高压柠檬酸盐(pH6.0)缓冲液+胃蛋白酶K(10mg/L)的联合修复方法,并采用1%Tri...     

Effect of Kruppel-like factor 4 on Notch pathway in hepatic stellate cells

The relationship between Kruppel-like factor 4 (KLF4) and the Notch pathway was determined to investigate the effect of KLF4 on the activation of hepatic stellate cells and underlying mechanisms. Fifty SPF BALB/c mice were randomly divided into two groups. A liver fibrosis model was established in 25 mice as the experimental group, and the remaining 25 mice served as controls. On the day 0, 7, 14, and 35, liver tissues were removed for immunofluorescent detection. The Notch pathway inhibitor DAPT was added to the primary original hepatic stellate cells, and KLF4 and Notch-associated factor expression was detected by qRT-PCR. Additionally, the hepatic stellate cell line LX-2 was used to establish control and experimental groups, and was cultured in vitro. LX-2 cells in the experimental groups were treated with DAPT and the Notch activator transforming growth factor-beta 1 separately, whereas those in the control group were given isotonic culture medium. After 48 h, KLF4 expression was examined by Western blotting. After transient transfection of LX-2 cells to increase KLF4, the expression of Notch factor was examined. Immunofluorescence analysis showed that, with the aggravation of liver fibrosis, the absorbance (A) values of KLF4 were decreased (day 0: 980.73±153.19; day 7: 1087.99±230.23; day 14: 390.95±93.56; day 35: 245.99±87.34). The expression of Notch pathway- related factors (Notch-1, Notch-2, and Jagged-1) in the hepatic stellate cell membrane was negatively correlated to KLF4 expression. With the increase of KLF4 expression, Notch-2 (0.73±0.13) and Jagged-1 (0.43±0.12) expression decreased, whereas Notch-1 level was not detectable. When the Notch pathway was inhibited, KLF4 levels generally increased (18.12±1.31). Our results indicate that KLF4 expression is negatively correlated to the Notch pathway in hepatic stellate cells, which may provide a reference for the treatment of hepatic fibrosis.     

DNA修复蛋白磷酸化组蛋白H2AX在胃癌中的表达及其意义

DNA双链断裂(DSBs)是真核生物后果最严重的DNA损伤之一[1].如果DSBs不能及时而准确地修复,可能导致基因突变、基因组不稳定、细胞凋亡甚至癌症的发生[2].磷酸化H2AX(γ-H2AX)是启动DSBs修复的关键因子,其在维持基因组稳定性方面具有重要作用[3].已有研究结果表明γ-H2AX在多种肿瘤中异常表达,但在胃癌中的研究不多本研究旨在观察γ-H2AX在胃癌的表达,并探讨其临床意义. 一、材料和方法 1.一般资料:收集2006年1月至2008年10月于广西医科大学胃肠腺体外科进行手术切除的胃癌标本30例,其中男16例,女14例;年龄≤60岁18例,年龄＞60岁12例,平均年龄53岁.所有患者术前未接受化疗、放疗、免疫治疗以及其他针对肿瘤的治疗.按照国际抗癌联盟1997年TNM分期标准进行肿瘤分期.γ-H2AX抗体、β-肌动蛋白(β-actin)抗体购自美国Abcam公司;SP即用型试剂盒购自北京中杉金桥生物公司.