Effects of Bu-Shen-An-Tai recipe and its two components on endometrial morphology during peri-implantation in superovulated mice

The aim of this study was to investigate the effect of Bu-Shen-An-Tai recipe （BSATR） and its two components （Bushen recipe, and Huoxue recipe） on endometrial morphology during peri-implantation in superovulated mice. Mice were randomly divided into five groups, including the normal （N）, model （M）, Bushen （BS）, Huoxue （HX） and Bu-Shen-An-Tai （BH） groups. The uteri were collected on day 4 of pregnancy, and the endometrium thickness, microvessel density （MVD） and number of pinopodes observed. Compared with the M group, the endometrial thickness in the BS, HX and BH groups was significantly increased and there was a significant difference in endometrial thickness between the BS and the BH groups. The mean MVD was significantly lower in the M group than in the N group, and there was a significant increase in MVD in the BS, HX and BH groups as compared with the M group. Compared with the M group, the pinopode scores in the en- dometrium were significantly increased in the HX and BH groups; and the BS group had significantly higher pinipode scores than the HX and BH groups. In conclusion, the results of the present study demonstrated that the recipes （Bushen, Huoxue and BSATR） could improve the endometrial envi- ronment by regulating the endometrial thickness, MVD and the number of pinopodes at the window of implantation. Moreover, the Huoxue recipe and the BSATR were more efficient than the Bushen recipe, with the BSATR tending to have the most beneficial effects.     

De-qi, not psychological factors, determines the therapeutic efficacy of acupuncture treatment for primary dysmenorrhea

<正>Objective:To study the impact of De-qi(得气,obtaining qi) and psychological factors on the efficacy of acupuncture treatment for primary dysmenorrhea,with an attempt to explore the relationship among De-qi, psychological factors,and clinical efficacy.Methods:The patients with primary dysmenorrhea were randomly assigned to a group of acupuncture with manual manipulation(manipulation group,n=67) and an acupuncture group without manipulation(non-manipulation group,n=64).Pain intensity and pain duration were used as measures for evaluating the therapeutic efficacy of the acupuncture treatment.De-qi,the sensations a patient experienced during the acupuncture treatment,was scored on a 4-point scale by the subjects.In addition,the psychological factors,including belief in acupuncture,the level of nervousness,anxiety,and depression,were quantitatively assessed.The personality of the subject was assessed using the Eysenck personality questionnaire (EPQ) and 16 personality factor questionnaire(16PF).Results:Complete data were obtained from 120 patients,60 patients in each group.There were statistically significant differences in pain intensity(W=2410.0,P0.01) and pain duration(W=3181.0,P0.01) between the two groups.The number of De-qi acupoints(W=1150.5,P0.01) and the average intensity of De-qi(W=1141.0,P0.01) were significantly higher in the manipulation group as compared with their non-manipulation counterparts.The correlation coefficients between De-qi and therapeutic efficacy of acupuncture were greater than those between psychological factors and therapeutic efficacy.Conclusions: Compared with the psychological factors,De-qi contributed more to the pain-relieving effect of acupuncture in subjects with primary dysmenorrhea.Moreover,manual manipulation is a prerequisite for eliciting and enhancing the De-qi sensations,and De-qi is critical for achieving therapeutic effects.     

兔VX2肝种植瘤盐酸消融治疗两周后CT灌注参数分析

目的:肝癌的盐酸消融治疗是一种局部、微创、安全、可以重复进行的治疗方法,与手术相比,能减少对正常组织的损伤,避免开腹手术对患者的打击,减少手术相关发病率和死亡率,并且花费低、住院时间短.盐酸消融对高风险、不能手术的患者很大程度上能起到代替手术的作用.目前,在评估盐酸消融疗效方面还存在争议,但是随着技术的进步和经验的累积,盐酸消融必将在腹部肿瘤的治疗中有着更加广阔的前景.本实验通过建立兔VX2肝种植瘤模型探讨了不同时相VX2肿瘤的CT灌注参数特征,为进一步评价功能影像学在肝种植瘤盐酸消融疗效预测提供实验依据,探讨血流动力学参数对盐酸消融治疗疗效预测的可能性及血流动力学参数变化的特点.本实验通过观察兔肝VX2种植瘤盐酸消融治疗2周后CT灌注参数的变化及盐酸消融组(治疗组)与空白对照组(对照组)CT灌注参数的变化进而为临床肝癌治疗提供科学的依据.方法:新西兰大白兔20只,采用组织块种植法制成兔肝VX2模型,随机分为治疗组和对照组,2周后治疗组兔接受盐酸消融治疗.于治疗后14 d分别对两组行CT灌注扫描动态监测.选取肿瘤中央区、周边区和瘤周正常肝组织的感兴趣区(ROI),分别测量灌注值(PV)、强化峰值增量(PEI)、达峰值时间(TTP)、血容量(BV).统计学处理采用独立样本的t检验及自身配对t检验分析治疗组与对照组及每组自身灌注参数的变化.结果:治疗组与对照组肿瘤中央区及瘤周区PV、PEI、BV存在明显差异(P＜0.05);治疗组与对照组肿瘤中央区及瘤周区TTP无显著差异(P＞0.05).结论:PV、PEI、BV灌注参数的变化可以反映兔VX2肝移植瘤内部血流动力学的差异,早期区别肿瘤细胞血流稀疏区和增殖旺盛的集区,为从微观角度观察兔VX2肝移植瘤盐酸消融提供了基础,对临床盐酸消融患者提高生活质量及远期预后意义重大.     

针刺对胚胎着床障碍大鼠子宫内膜CCR2的影响

目的观察针刺对胚胎着床障碍大鼠子宫内膜趋化因子受体CCR2表达的影响。方法将72只孕鼠随机分为正常组（N ）、胚胎着床障碍组（M ）、针刺治疗组（A ）和黄体酮治疗组（W ）,每组随机再分为第6天组（D6）、第8天组（D8）、第10天组（D10）。其中M 组、A 组和W 组大鼠妊娠第1天给予米非司酮麻油溶液造模,N 组仅给予等量麻油溶液。同时,A 组固定并每天针刺三阴交、后三里,N 组和M 组仅固定,W 组肌肉注射黄体酮,直至处死。用免疫组织化学法、Western blot 和 Real-time PCR等方法测定着床点子宫内膜 CCR2的表达。结果与 N 组相比,M 组大鼠子宫内膜 CCR2蛋白和mRNA的表达均明显降低,差异有统计学意义（P＜0.05）;与 M 组相比,A 组和 W 组大鼠子宫内膜CCR2蛋白和 mRNA 的表达则明显增高（P＜0.05）。结论针刺能上调胚胎着床障碍大鼠母胎界面CCR2的表达,从而促进胚胎的着床和胎盘的形成。     

针刺对克罗米芬治疗的多囊卵巢综合征大鼠子宫内膜容受性的影响

目的 观察针刺对促排卵治疗的多囊卵巢综合征(PCOS)大鼠子宫内膜容受性的影响,探讨其作用机制.方法 对24日龄雌性SD大鼠皮下注射脱氢表雄酮(DHEA)的麻油溶液制作多囊卵巢综合征(PCOS)大鼠模型,对照组(Con)同期皮下注射麻油.PCOS大鼠随机分为模型组(M)、克罗米芬组(C)、克罗米芬+针刺组(C+A),其中M组不作任何处理,其它各组均于80日龄起进行干预5 d.治疗结束后,各组部分大鼠断头处死,留取子宫和子宫内膜标本.以RT-PCR法检测各组大鼠子宫内膜雌激素受体(ER)、孕激素受体(PR)、同源盒基因A10(HOXA10)、白血病抑制因子(LIF)mRNA表达,Western blot法检测ER、PR、HOXA10、LIF及整合素αvβ3(Integrin αvβ3)蛋白的表达.各组其余大鼠与雄鼠交配,于妊娠第8天处死,观察大鼠胚泡着床情况.结果 与C组相比,C+A组大鼠子宫内膜发育更好,上述子宫内膜容受性的标志分子蛋白及mRNA表达水平也更接近对照组(P<0.05).结论 针刺能显著改善克罗米芬促排卵治疗导致的子宫内膜容受性不良状态,可能通过调节子宫内膜容受性的标志分子蛋白及mRNA表达而实现.     

补肾安胎方及其拆方对着床障碍小鼠胚泡着床疗效观察

目的:观察补肾安胎方及其构方中补肾组分、益气活血组分对胚泡着床障碍小鼠胚泡着床位点数的影响。方法:将妊娠小鼠随机分为正常组、模型组、补肾安胎组、补肾组及益气活血组。妊娠第3、4天,正常组小鼠每天2次皮下注射麻油0.12ml,其余四组皮下注射吲哚美辛油剂0.12ml,造成小鼠胚泡着床障碍的动物模型。分别收集妊娠第5、6、8天小鼠子宫,计数着床位点个数并比较其大小形态。结果:补肾安胎组小鼠着床位点数与模型组比较显著提高(P<0.05),补肾组和益气活血组小鼠着床位点数较模型组多,但无统计学意义;补肾组和益气活血组小鼠着床位点数较补肾安胎组少,但无统计学意义。第5、6天着床位点胚泡,正常组和补肾安胎组左右两侧子宫分布均匀,胚泡大;模型组胚泡分布明显不均,且比正常组及补肾安胎组小;补肾组和益气活血组胚泡分布尚均匀,比正常组及补肾安胎组稍小。妊娠第8天正常组和补肾安胎组胚泡大而且各胚泡大小均等,形状规则,胚泡间界限清楚,无相互融合现象;模型组胚泡大小形状不一,各胚泡之间发生融合;补肾组和益气活血组胚泡大小较均匀,但有融合现象。结论:集补肾和益气活血为一体的补肾安胎方可以明显提高小鼠着床位点数,改善胚泡的形态和宫内的分布。     

浅谈中西医结合专病专科提高中药疗效的思路

临床疗效是决定中医学延续和发展的根本问题，随着医学和社会的发展，中医面临新的挑战，适应市场变化，革新思路，提高中药疗效，是解决中医人目前生存问题的根本途径。由于中西医各自所具有的特色，使西医辨病和中医辨证的诊疗理念已被中医界、中西医结合界和病人普遍接受，这不仅给我们提出了更高的要求，同时也给我们提供了前所未有的机会。理论上我们需要的是精通中、西医理论，通晓中、西医临床治疗方法的医生，但是，由于一个医生一生的精力有限，很难做到精益求精：同时这些慢性、难治病病人实际需要的是在某领域，卜精通中西医理论和临床的医生。因此，发展中西医结合专病专科，有利于病人，有利于医生，有利于发现更加有效的中西医结合治疗方案，本文仅从中西医结合角度谈谈如何组方以提高中药疗效。     

针刺三阴交对痛经患者脑葡萄糖代谢的影响

目的：探讨针刺三阴交治疗痛经的中枢作用机制。方法：对6例痛经患者行经疼痛时行右侧三阴交假针刺和针刺，用数字疼痛强度分级法比较刺激前后疼痛情况，并在假针刺和针刺时利用正电子发射断层摄影技术（positron emission tomograph，PET）对痛经患者行埔F—FDG脑功能成像，用SPM软件分析，获得针刺激活的脑解剖功能区。结果：假针刺前后疼痛值差异无显著性意义（P〉0．05），针刺三阴交后疼痛值比针刺前明显降低（P〈0．01）。针刺右侧三阴交引起痛经患者多个与疼痛相关脑区被激活，针刺使同侧豆状核（苍白球、壳）、同侧小脑、同侧岛叶、双侧背侧丘脑、同侧中央旁小叶、双侧杏仁体、对侧中脑黑质、双侧第Ⅱ躯体感觉区、同侧海马回、同侧扣带回前部、对侧下丘脑乳头体葡萄糖代谢增强，大脑皮质小区域葡萄糖代谢降低。结论：针刺三阴交能明显缓解痛经患者疼痛，其机理为针刺三阴交可激活皮质、皮质下边缘系统和小脑与疼痛相关脑区，可能通过平衡与疼痛有关的中枢网络而减轻疼痛，神经内分泌也可能在治疗中起作用。     

针刺对经脉穴位微循环血流量的影响

目的:探讨针刺对经穴及非经穴微循环血流量的影响及其机制。方法:应用激光多普勒血流仪(LaserDopplerFlowmeter,LDF)检测30例健康人针刺前后十二经脉循经经穴、经穴旁开和在经非穴微循环血流量的变化。结果:人体穴位血流量高于经穴旁开和在经非穴(P<0.05);针刺时,十二经脉循经线上经穴的微循环血流量明显增加,经穴旁开的血流量增减不明显,两者相比有显著性差异(P<0.05);针刺时,十二经脉在经非穴的血流量虽有增加,但无统计学意义,经穴的血流量与在经非穴的血流量相比,具有显著性差异(P<0.05)。结论:经络的循经感传现象是可以客观检测的,微循环检测可以为经络的生理机制研究提供更多信息。     

原发性痛经患者18F-FDG PET脑显像研究

本研究通过比较原发性痛经和健康者^18F-脱氧葡萄糖（FDG）PET脑显像,探讨原发性痛经的中枢机制,现报道如下.