POLO-LIKE KINASEl GENE EXPRESSION AND ITS CLINICO-PATHOLOGICAL SIGNIFICANCE IN GASTRIC CARCINOMA

Objective To clarify the polo-like kinasel (PLKI) expression in human gastric cancer tissue and its clinicopathological significance in gastric carcinoma. Methods PLKl expression in 60 cancer tissues and their corresponding noncancerous tissues from gastric cancer patients was measured by both real-time quantitative RT-PCR and western blot assay. Immunohistochemistry was used to detect PLKI protein expression in eighty-nine paraffin-embedded samples. Results The PLKI mRNA and protein expression level in the 60 fresh cancer tissues was significantly higher than that in noncancerous tissues (P <0. 0001, P =0. 031 respectively). In paraffin-embedded samples, apart from its increased expression level, PLKI was found to be in both cytoplasm and nucleus, double-site location only occurred in poor-differentiated cancer, PLKI expression intensity was associated with tumor differentiation (P = 0. 03) , invasion (P = 0. 032) , TNM stage (P=0.019), ki67 expression (P =0.011). The patients with negative PLKl expression had better survival rate than that with positive PLKl expression (P = 0. 0292). Conclusion PLKl may have clinicopathological value in tumor diagnosis, and may become another new bio-marker and therapeutic target for gastric cancer.     

TAGLN在胃癌组织中的表达及其与转移的关系

目的 观察TAGLN在胃癌中的表达及其与肿瘤细胞侵袭转移能力的关系.方法 运用实时PCR、Western blotting及免疫组化方法,检测TAGLN在40例胃癌患者肿瘤组织和相应癌旁组织、7株胃癌细胞株及癌相关成纤维细胞(CAFs)中的表达;分析TAGLN的表达与胃癌临床病理学之间的关系.运用细胞侵袭和移动试剂盒检测高表达TAGLN的CAFs或沉默TAGLN表达后的CAFs对胃癌细胞株MKN45侵袭及移动能力的影响.结果 在mRNA和蛋白表达水平方面,各胃癌细胞株间无显著性差异(P＞0.05);与相应癌旁组织相比,胃癌组织TAGLN的表达明显上调(P＜0.05);免疫组化染色显示,TAGLN蛋白表达于胃癌组织间质中的成纤维细胞、新生血管内皮细胞及肿瘤浸润的黏膜肌层中的肌细胞,且主要表达于细胞膜和细胞浆.淋巴结转移组胃癌组织TAGLN蛋白的表达为3.751±0.681,显著高于无淋巴结转移组的1.084±0.328(P＜0.05).细胞侵袭和移动试验结果显示,高表达TAGLN的CAFs能够促进胃癌细胞株MKN45的侵袭及移动能力;而经siRNA沉默TAGLN表达后的CAFs则明显降低胃癌细胞株MKN45的侵袭及移动能力.结论 TAGLN基因在胃癌组织的基质中高表达,其表达上调对胃癌的侵袭和转移可能具有促进作用.     

胃癌及癌旁组织多个肿瘤相关基因超甲基化

目的 检测胃癌及癌旁组织肿瘤相关基因超甲基化状态,探讨多个肿瘤相关基因启动子超甲基化与胃癌发生的关系.方法 应用甲基化特异性聚合酶链反应,检测23例手术切除的胃癌组织、癌旁组织和10例胃镜活检正常的胃黏膜组织的hMLH1、E-cadherin、GSTP1、p16和p15基因启动子区域的超甲基化状态.结果 正常胃黏膜组织未检测到所选基因的超甲基化,22例(95.7%)胃癌组织和7例(30.4%)癌旁组织中检测到超甲基化.在17例(73.9%)胃癌组织和5例(21.7%)癌旁组织可以检测到2个以上基因的超甲基化;E-cadherin、p15和 p16 三个基因的甲基化率分别为78.3%、82.6%、60.8%,明显高于hMLH1和GSTP1的34.8%和17.4%.结论 胃癌组织和邻近的癌旁组织中可以同时检测到多个肿瘤相关基因的甲基化,启动子区域超甲基化导致的基因功能失活可能发生在胃癌肿瘤形成的早期.     

运动与严重持续性精神病患者：群组步行运动将是降低该病的有效方法

精神疾病，如精神分裂症、抑郁症双相障碍，强迫症、惊恐障碍，伴有无法工作，不能独立生活等功能缺陷时，我们把它归到严重、持续性精神病。据不完全统计，数百万的美国成人患有严重持续性精神病（以后称之为严重精神病），它已经成为残疾的首要因素，据国家精神卫生研究所的数据，在1年里，1480万人患有重度抑郁症，600万人患有惊恐障碍，570万人患有双相障碍，240万人患有精神分裂症，220万人患有强迫症。患有严重精神症的人患其他疾病的风险会增加，如高血压、糖尿病、心脏病和肥胖等，这些逐渐增加的风险与生活方式有关，例如患有严重精神疾病的病人喜欢吸烟，不愿参加各种轻、重度体育锻炼，这和精神药物的副反应有关。     

胃癌及癌旁组织多个肿瘤相关基因超甲基化

目的检测胃癌及癌旁组织肿瘤相关基因超甲基化状态,探讨多个肿瘤相关基因启动子超甲基化与胃癌发生的关系。方法应用甲基化特异性聚合酶链反应,检测23例手术切除的胃癌组织、癌旁组织和10例胃镜活检正常的胃黏膜组织的hMLH1、E-cadherin、GSTP1、p16和p15基因启动子区域的超甲基化状态。结果正常胃黏膜组织未检测到所选基因的超甲基化,22例(95.7%)胃癌组织和7例(30.4%)癌旁组织中检测到超甲基化。在17例(73.9%)胃癌组织和5例(21.7%)癌旁组织可以检测到2个以上基因的超甲基化;E-cadherin、p15和p16三个基因的甲基化率分别为78.3%、82.6%、60.8%,明显高于hMLH1和GSTP1的34.8%和17.4%。结论胃癌组织和邻近的癌旁组织中可以同时检测到多个肿瘤相关基因的甲基化,启动子区域超甲基化导致的基因功能失活可能发生在胃癌肿瘤形成的早期。     

In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity

Purpose:The metallopanstimulin-1(MPS-1)gene is a growth factor-inducible gene,which is highly expressed in many human cancers and may be involved in the progression towards tumor malignancy.However,it is unclear whether MPS-1 plays any role in gastric cancer development or progression.Our studies were designed to clarify the MPS-1 expression pattern and to explore its potential role in gastric cancer.Experimental Design:The expression pattern of MPS-1 was determined in primary gastric cancer specimens and gastric cancer cell lines via immunohistochemistry and Western blotting.To investigate the functional significance of MPS-1 expression,three small interfering RNA(siRNA)expression plasmids were constructed and transfected into gastric cancer cell line SGC7901.The stable cell lines transfected with the siRNA targeting MPS-1 mRNA plasmids were selected and the biological features of these cells were examined.Results:MPS-1 was overexpressed in 86% of the gastric cancer tissues and all gastric cancer cells.In addition,MPS-1 expression was significantly increased and corresponded with the tumor-node-metastasis clinical stage,and was significantly higher in the late stage(P<0.01).The MPS-1 expression level was significantly decreased in the transfected cells with MPS-1-specific siRNA expression plasmid pRNAT-133.Furthermore,the stable transfected cancer cells exhibited an increase in the incidence of spontaneous apoptosis and a decrease in growth ability and tumorigenicity in nude mice.Conclusions:These results provide strong evidence that MPS-1 plays an important role in gastric cancer cell proliferation and development,and suggests that MPS-1 is a promising target for gastric cancer treatment.     

保罗样激酶1mRNA及蛋白质在胃癌中的表达意义

目的 探讨保罗样激酶1（plk1）在胃癌组织中mRNA及蛋白质的表达，并探讨其表达水平与临床病理指标的关系。方法 采用实时定量多聚酶链反应及免疫印迹（Western blot）分别检测了60例胃癌患者新鲜切除胃癌组织及其对应正常胃粘膜组织中plk1 mRNA及蛋白质的表达。结果胃癌组织中plk1 mRNA及蛋白质水平均明显高于正常组织（P〈0．05，P〈0．01）；并且其水平与胃癌的分化程度和浸润深度有关（P〈0．05）；蛋白质表达水平与胃癌的分化有关（P〈0．05）。结论 plk1的过表达可能在胃癌发生发展中起促进作用；其表达程度可望作为胃癌的某些生物学行为新的判定指标。     

STK15和ki67基因在胃癌组织中的表达研究

目的研究丝氨酸/苏氨酸激酶15 (STK15)基因在胃癌组织中的表达,及其与临床病理指标以及ki67表达的关系.方法采用免疫组化检测STK15和ki67在89例胃癌组织中的表达.结果 STK15在胃癌组织中的表达阳性率为34.83%(31/89),显著高于邻近的非癌组织的4.49%(12/89)(P<0.01);STK15基因过表达与分化型胃癌及其侵润深度密切相关(P<0.05),而与ki67的表达无相关性.结论 STK15基因可能在胃癌发生、发展中起相应作用,其过表达程度可作为胃癌的某些生物学行为新的判定指标.     

小干扰RNA下调GLI1基因的表达对MKN28细胞转移侵袭行为的影响

目的利用siRNA技术下调GLI1基因的表达,在体外研究GLI1对MKN28胃癌细胞侵袭转移能力的影响。方法脂质体法将GLI1 siRNA转染MKN28胃癌细胞,采用RT-PCR观察GLI1 siRNA转染前后GLI1在mRNA水平的变化情况。培养MKN28胃癌细胞,将细胞分为转染GLI1 siRNA组、阴性siRNA组和对照组后,分别采用Transwell小室法检测各组细胞的侵袭和转移能力。结果转染GLI1 siRNA后GLI1基因表达在24 h和48 h明显下调。侵袭和转移实验显示:GLI1 siRNA组侵袭和转移细胞数目均低于阴性siRNA组和对照组(P<0.05),而阴性siRNA组和对照组间的差异均无统计学意义。结论转染GLI1 siRNA特异下调GLI1基因表达,在体外抑制胃癌MKN28细胞的侵袭和转移,可能是胃癌靶向治疗的靶点。     

急性胰腺炎时骨髓和循环内皮祖细胞的变化研究

Objective To investigate changes in number of endothelial progenitor cells(EPCs)from bone marrow and circulation in mice with acute pancreatitis.Methods BALB/c mice were assigned randomly to saline group and cerulein group.Animals were sacrificed at 12, 24 and 48 hours after injection.Bone marrow and circulating EPCs were detected by flow cyzometric analysis.Plasma VEGF, TNF-α and ET-1 were determined by enzyme-linked immunosorbent assay.The expression of VEGF in the pancreas was assessed by Western blotting.Apoptosis in situ was detected by TUNEL.Results The amounts of EPCs in bone marrow and circulation increased remarkably after cerulein injection(P < 0.05), also the levels of plasma VEGF TNF-α and ET-1(P < 0.05), the EPCs levels in bone marrow and circulation seen in the study closely mirrors the levels of VEGF detected in the circulation(r = 0.77, 0.67 individually).VEGF expression in pancreas was up-regulated after 12 h of cerulein injection compared with that of control group.Apoptosis of endothelial cells also increased in the cerulein group.Conclusion EPCs were mobilized by acute pancreatitis, which may be due to the mobilizing effect of increased levels of VEGF, EPCs may participate in the repair process of injured endothelium induced by acute pancreatitis.