Hedgehog信号通路在胃癌细胞中活化并通过GLI1促进MKN28细胞增殖

目的研究Hedgehog：（HH）信号通路在胃癌细胞中的活化形式及其转录因子GLI1对胃癌细胞增殖和体外成瘤能力的影响。方法通过反转录PCR分析HH通路相关组成分子在7株细胞系中的表达情况．化学合成针对HH信号通路转录因子GLI1的siRNA并转染胃癌MKN28细胞。通过CCK8法和体外克隆形成实验．观察GLII siRNA转染胃癌MKN28细胞后的细胞增殖能力和克隆形成能力的变化。结果在不同胃癌细胞株中．HH信号通路各相关基因的表达情况存在差异,SHH在6株胃癌细胞系中均表达上调．PTCH在KATOⅢ细胞系、SUFU在MKN28和KATOⅢ表达下调。转染GLI1 siRNA后,MKN28细胞中GLI1 mRNA表达受到明显抑制;经GLI1 siRNA作用的MKN28细胞生长明显缓于阴性对照和未处理组（P=0．014）,克隆形成数目及体外克隆形成能力降低（P〈0．001）。结论HH信号通路在胃癌细胞系中被普遍激活,HH信号通路活化后可通过转录因子GLI1促进MKN28细胞增殖．     

B族链球菌液固双相显色培养基的临床应用研究

目的 通过与荧光PCR法结果进行比对,评价B族链球菌液固双相显色培养基的临床应用价值.方法 采集孕晚期孕妇的阴道及肛门样本共1026例,通过液固双相显色培养基和PCR方法进行检测,结果不一致时使用质谱仪进行验证.结果 PCR方法检出阳性82例,阳性率为7.99％ (82/1026).液固双相显色培养基检测阳性76例,阳...     

小干扰RNA下调GLI1基因的表达对MKN28细胞转移侵袭行为的影响

目的利用siRNA技术下调GLI1基因的表达,在体外研究GLI1对MKN28胃癌细胞侵袭转移能力的影响。方法脂质体法将GLI1 siRNA转染MKN28胃癌细胞,采用RT-PCR观察GLI1 siRNA转染前后GLI1在mRNA水平的变化情况。培养MKN28胃癌细胞,将细胞分为转染GLI1 siRNA组、阴性siRNA组和对照组后,分别采用Transwell小室法检测各组细胞的侵袭和转移能力。结果转染GLI1 siRNA后GLI1基因表达在24 h和48 h明显下调。侵袭和转移实验显示:GLI1 siRNA组侵袭和转移细胞数目均低于阴性siRNA组和对照组(P<0.05),而阴性siRNA组和对照组间的差异均无统计学意义。结论转染GLI1 siRNA特异下调GLI1基因表达,在体外抑制胃癌MKN28细胞的侵袭和转移,可能是胃癌靶向治疗的靶点。     

STK15和ki67基因在胃癌组织中的表达研究

目的研究丝氨酸/苏氨酸激酶15 (STK15)基因在胃癌组织中的表达,及其与临床病理指标以及ki67表达的关系.方法采用免疫组化检测STK15和ki67在89例胃癌组织中的表达.结果 STK15在胃癌组织中的表达阳性率为34.83%(31/89),显著高于邻近的非癌组织的4.49%(12/89)(P<0.01);STK15基因过表达与分化型胃癌及其侵润深度密切相关(P<0.05),而与ki67的表达无相关性.结论 STK15基因可能在胃癌发生、发展中起相应作用,其过表达程度可作为胃癌的某些生物学行为新的判定指标.     

保罗样激酶1mRNA及蛋白质在胃癌中的表达意义

目的 探讨保罗样激酶1（plk1）在胃癌组织中mRNA及蛋白质的表达，并探讨其表达水平与临床病理指标的关系。方法 采用实时定量多聚酶链反应及免疫印迹（Western blot）分别检测了60例胃癌患者新鲜切除胃癌组织及其对应正常胃粘膜组织中plk1 mRNA及蛋白质的表达。结果胃癌组织中plk1 mRNA及蛋白质水平均明显高于正常组织（P〈0．05，P〈0．01）；并且其水平与胃癌的分化程度和浸润深度有关（P〈0．05）；蛋白质表达水平与胃癌的分化有关（P〈0．05）。结论 plk1的过表达可能在胃癌发生发展中起促进作用；其表达程度可望作为胃癌的某些生物学行为新的判定指标。     

运动与严重持续性精神病患者：群组步行运动将是降低该病的有效方法

精神疾病，如精神分裂症、抑郁症双相障碍，强迫症、惊恐障碍，伴有无法工作，不能独立生活等功能缺陷时，我们把它归到严重、持续性精神病。据不完全统计，数百万的美国成人患有严重持续性精神病（以后称之为严重精神病），它已经成为残疾的首要因素，据国家精神卫生研究所的数据，在1年里，1480万人患有重度抑郁症，600万人患有惊恐障碍，570万人患有双相障碍，240万人患有精神分裂症，220万人患有强迫症。患有严重精神症的人患其他疾病的风险会增加，如高血压、糖尿病、心脏病和肥胖等，这些逐渐增加的风险与生活方式有关，例如患有严重精神疾病的病人喜欢吸烟，不愿参加各种轻、重度体育锻炼，这和精神药物的副反应有关。     

In vitro and in vivo evidence of metallopanstimulin-1 in gastric cancer progression and tumorigenicity

Purpose:The metallopanstimulin-1(MPS-1)gene is a growth factor-inducible gene,which is highly expressed in many human cancers and may be involved in the progression towards tumor malignancy.However,it is unclear whether MPS-1 plays any role in gastric cancer development or progression.Our studies were designed to clarify the MPS-1 expression pattern and to explore its potential role in gastric cancer.Experimental Design:The expression pattern of MPS-1 was determined in primary gastric cancer specimens and gastric cancer cell lines via immunohistochemistry and Western blotting.To investigate the functional significance of MPS-1 expression,three small interfering RNA(siRNA)expression plasmids were constructed and transfected into gastric cancer cell line SGC7901.The stable cell lines transfected with the siRNA targeting MPS-1 mRNA plasmids were selected and the biological features of these cells were examined.Results:MPS-1 was overexpressed in 86% of the gastric cancer tissues and all gastric cancer cells.In addition,MPS-1 expression was significantly increased and corresponded with the tumor-node-metastasis clinical stage,and was significantly higher in the late stage(P<0.01).The MPS-1 expression level was significantly decreased in the transfected cells with MPS-1-specific siRNA expression plasmid pRNAT-133.Furthermore,the stable transfected cancer cells exhibited an increase in the incidence of spontaneous apoptosis and a decrease in growth ability and tumorigenicity in nude mice.Conclusions:These results provide strong evidence that MPS-1 plays an important role in gastric cancer cell proliferation and development,and suggests that MPS-1 is a promising target for gastric cancer treatment.     

阿立哌唑单药与联合碳酸锂治疗双相障碍I型躁狂发作的对照研究

目的：比较阿立哌唑单药与联合碳酸锂治疗双相I型躁狂发作患者的临床疗效和安全性。方法将58例患者随机分为阿立哌唑组与联合碳酸锂组,于治疗前和治疗第1、2、4、6周末分别采用躁狂状态评定量表（BRMS）、临床疗效总评量表(CGI)、副反应量表(TESS)评定疗效及不良反应。于治疗前及治疗6周末各检测1次血常规、尿常规,肝、肾功能,血糖,血锂浓度、血脂,心电图、脑电图,以评价其安全性。结果试验组和对照组的BRMS 和CGI得分随着治疗时间而逐渐下降,治疗前后差异有明显统计学意义（P＜0.01）;两组间比较显示,单药组和联合用药组之间在第2周末差异有统计学意义（P<0.05）,在第4、6周末两组间比较差异有统计学意义（P<0.05）,在痊愈率方面也明显低于联合碳酸锂组,两组间的比较差异有统计学意义,两组均未出现严重的不良事件。结论阿立哌唑组治疗双相障碍I型躁狂发是安全有效的;阿立哌唑联合碳酸锂组有效率和痊愈率均优于阿立哌唑单药组。     

急性胰腺炎时骨髓和循环内皮祖细胞的变化研究

Objective To investigate changes in number of endothelial progenitor cells(EPCs)from bone marrow and circulation in mice with acute pancreatitis.Methods BALB/c mice were assigned randomly to saline group and cerulein group.Animals were sacrificed at 12, 24 and 48 hours after injection.Bone marrow and circulating EPCs were detected by flow cyzometric analysis.Plasma VEGF, TNF-α and ET-1 were determined by enzyme-linked immunosorbent assay.The expression of VEGF in the pancreas was assessed by Western blotting.Apoptosis in situ was detected by TUNEL.Results The amounts of EPCs in bone marrow and circulation increased remarkably after cerulein injection(P < 0.05), also the levels of plasma VEGF TNF-α and ET-1(P < 0.05), the EPCs levels in bone marrow and circulation seen in the study closely mirrors the levels of VEGF detected in the circulation(r = 0.77, 0.67 individually).VEGF expression in pancreas was up-regulated after 12 h of cerulein injection compared with that of control group.Apoptosis of endothelial cells also increased in the cerulein group.Conclusion EPCs were mobilized by acute pancreatitis, which may be due to the mobilizing effect of increased levels of VEGF, EPCs may participate in the repair process of injured endothelium induced by acute pancreatitis.     

小鼠骨髓来源内皮祖细胞体外诱导培养及分化特征

目的: 研究小鼠骨髓来源内皮祖细胞(EPCs)的体外培养方法及体外分化特征。方法: 采用梯度离心法分离小鼠骨髓单个核细胞并进行诱导培养,用流式细胞术和免疫细胞化学对细胞表型特征进行检测,分别采用MTT和Transwell方法对其增殖、迁移能力进行检测。结果: 体外培养4 d至14 d,CD133、SCA-1及CD117的表达率分别从11.05%、29.32%及16.45 %降至2.29%、7.82%及3.92%;而VEGFR-2、CD31、VE-cadherin、eNOS及vWF的表达率分别从12.21%、8.43%、18.27%、7.11%及32.61%增加至62.13%、32.96%、67.73%、27.89%及82.16%;吞噬acLDL和结合UEA-I的细胞从57.18%增加至86.70%。在0 μg/L至80 μg/L VEGF浓度梯度下,细胞增殖率和迁移率分别增加58.03%和33.83%。结论: EGM-2内皮培养基可稳定培养小鼠骨髓来源EPCs,培养过程中其干细胞标志物表达逐渐降低,而内皮细胞标志物表达及特性不断增加,VEGF可显著促进其增殖和迁移。