Preparation and identification of monoclonal antibodies against Penicillium marneffei]

OBJECTIVE: To prepare and identify monoclonal antibodies (mAbs) against Penicillium marneffei. METHODS: Recombinant mannoprotein1 (MP1) of Penicillium marneffei was used to immunize BALB/c mice, and anti-MP1 mAbs were obtained by means of hybridoma. Screening and identification of the mAbs were subsequently performed with indirect enzyme-linked immunosorbent assay and Western blotting, respectively. RESULTS: Four hybridomas producing antibodies against Penicillium marneffei were obtained, and the IgG isotypes of the 4 mAbs were identified as IgG1 with affinity constants (K) of 8.2x10(-9), 4.7x10(-9), 6.5x10(-9) and 2.7x10(-9), respectively. Western blotting demonstrated specific recognition of Aspergillus fumigatus MP1 by the obtained mAbs. CONCLUSION: The 4 hybridomas producing anti-MP1 mAbs with high specificity and affinity can be of significant value in the diagnosis of Penicilliosis marneffei infections.     

经喉内镜手术治疗喉部疾病的并发症分析

目的：分析经喉内镜手术后并发症的发生原冈及其防治措施。方法：使用从德国STORZ公司引进的国内首套喉内镜手术系统，治疗包括声带小结、声带息肉、喉乳头状瘤、声带囊肿、会厌囊肿、喉部炎性假瘤、喉部血管瘤等58例患者，并随访2～36个月，观察主要并发症并作相应处理。结果：58例患者中发音正常者54例，发音功能明显好转者4例。主要并发症有：牙齿松动2例，牙齿脱落1例，声带粘连1例，舌体麻木1例，舌运动障碍1例，术后拔管时喉痉挛1例。结论：经喉内镜手术可住微创的前提下彻底清除病灶，并最大限度地保留发音功能，只要术前严格掌握适应证，术中精细操作，其并发症是可以避免的。     

Comparing diffusion-weighted and T2-weighted MR imaging for the quantification of infarct size in a neonatal rat hypoxic–ischemic model at 24 h post-injury

PURPOSE: In a neonatal rat model of hypoxic-ischemic (HI) brain injury, using T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI), we aim to determine the best MRI method of lesion quantification that reflects infarct size. MATERIALS AND METHODS: Twenty 7-day-old rats underwent MRI 24h after HI brain injury was induced. Lesion size relative to whole brain was measured using T2WI and apparent diffusion coefficient (ADC) maps, applying thresholds of 60%, 70% and 80% contralateral control hemisphere mean ADC, and at day 10 post-HI on pathology with TTC staining. Multiple linear regression analysis was used to study the relationships between lesion size at MRI and pathology. RESULTS: Lesion size measurement using all MRI methods significantly correlated with infarct size at pathology; using T2WI, r=0.808 (p<0.001), using 80% ADC, 70% ADC and 60% ADC thresholds, r=0.888 (p<0.001), 0.761, (p<0.001) and 0.569 (p=0.014), respectively. Eighty percent ADC threshold was found to be the only significant independent predictor of final infarct volume (adjusted R(2)=0.775). CONCLUSION: At 24h post-HI, lesion size on DWI, using 80% ADC threshold is the best predictor of final infarct volume. Although T2WI performed less well, it has the advantage of superior spatial resolution and is technically less demanding. These are important considerations for experiments which utilize MRI as a surrogate method for lesion quantification in the neonatal rat HI model.     

表皮干细胞的快速高效分离

目的 寻找一种快递高效分离组织工程化皮肤种子细胞-皮肤干细胞的方法.方法 取成人手术切除之包皮,利用Ⅰ型胶原酶结合胰蛋白酶消化法,快速分离表皮干细胞,并对其进行鉴定及相关的生物学特性的研究.结果 应用Ⅰ型胶原酶结合胰蛋白酶,可在3小时内快速分离到表皮干细胞及成纤维细胞,经流式细胞仪及细胞免疫荧光鉴定,表皮细胞群体中角蛋白19(K19)阳性表达的细胞即表皮干细胞约占18%,但是其体外短期扩增仍较困难.结论 利用胶原酶消化法可在短期内高效分离到用于构建组织工程化皮肤的种子细胞,这为快速构建组织工程化皮肤提供了可能.     

对称性颅脑损伤的临床诊断与治疗(附123例报告)

目的提高治疗对称性颅脑损伤临床疗效。方法总结123例对称性颅脑损伤患者的临床资料,对其临床特点和治疗情况进行回顾性分析。结果预后良好64例(52.0%),中残25例(20.3%),重残8例(6.5%),植物生存4例(3.3%),死亡22例(17.9%)。结论对称性颅脑损伤是一种较为危重的特殊损伤类型,重视其临床特点,严密观察病情变化,正确把握治疗适应证,方可提高疗效。     

胆汁对豚鼠离体胆囊肌条收缩影响的实验研究

目的：观察不同浓度的胆汁对胆囊平滑肌运动的影响并初步探讨其作用机理。方法：将肌条置于离体平滑肌灌流肌槽内，用生理记录仪记录肌条的收缩活动。结果：(1)胆汁可降低离体胆囊肌条张力，减慢收缩频率，减小收缩波平均振幅。(2)心得安、L—NNA、六烃季胺可部分阻断胆汁对脂囊肌条的作用。结论：胆汁对豚鼠胆囊肌条的作用与肾上腺素能β受体、胆碱能N受体和N0有关。     

反义脱氧寡核苷酸对SRS病毒复制的抑制作用

用合成的反义脱氧寡核苷酸,包括修饰的和非修饰的,同聚的和异聚的脱氧寡核苷酸对SRS病毒感染细胞的增殖、细胞群落形成,XC融合细胞产生的抑制作用。结果表明,上述的寡聚物对病毒都有抑制活性。各种寡聚物的作用机制不同。     

2型糖尿病患者高危因素分析

目的 分析2型糖尿病患者各种高危险因素控制状况及其与慢性并发症的关系。方法 记录464例50岁以上的2型糖尿病患者收缩压、舒张压、血脂、糖化血红蛋白和体质指数，按有无并发症分为两组进行分析。结果 有并发症组糖尿病病程、血压、总胆固醇、低密度脂蛋白胆固醇和血纤维蛋白原明显增高。Wald卡方分析显示糖尿病病程和收缩压有统计学意义。多数患者这些危险因素未得到很好的控制。结论 糖尿病患者存在着多种高危因素，有慢性并发症者更为严重，应注意综合治疗。     

人甲胎蛋白时间分辨免疫荧光分析试剂盒的研制

目的研制人甲胎蛋白(hAFP)时间分辨免疫荧光分析(TRFIA)试剂盒.方法采用双抗体夹心法建立AFP TRFIA 试剂盒,对试剂盒的各项指标进行评价.结果试剂盒的可测范围为l～1 000 U/ml,灵敏度为0.17 U/ml,精密度良好,批内和批间的精密度分别为3.3%～5.9%,3.7%～6.5%.与CEA、CA12-5、CA19-9、CA15-3、白蛋白无交叉反应.稳定性试验表明试剂可以在4℃稳定1年,37℃稳定7 d.426份正常血清标本测试该试剂盒的正常参考值范围是0～12 U/ml.用本试剂盒与国外同类试剂盒同时检测60份血清标本,其相关系数为0.995.结论试剂盒各项指标(灵敏度、精密度、特异性、稳定性、准确度)均达到临床检测要求,可替代国外同类产品试剂盒.