FOXP3在正常人皮肤和表皮肿瘤中的表达及意义

目的   探讨叉头状转录因子P3（forkhead box protein-3,FOXP3）在正常人皮肤和不同表皮肿瘤皮损中的表达及意义。方法   应用免疫组织化学EnVision法检测20例脂溢性角化、19例日光性角化、21例鲍恩病、13例鳞状细胞癌及13例正常人皮肤中FOXP3的表达。结果   脂溢性角化、日光性角化、鲍恩病、鳞状细胞癌皮损中FOXP3+淋巴细胞阳性率均高于正常皮肤（P＜0.01）;脂溢性角化、日光性角化、鲍恩病、鳞状细胞癌间FOXP3+淋巴细胞阳性率差异有统计学意义（P＜0.01）;日光性角化FOXP3+淋巴细胞阳性率高于脂溢性角化（P＜0.05）;鲍恩病、鳞状细胞癌FOXP3+淋巴细胞阳性率均高于日光性角化（P＜0.05）,而其在鲍恩病与鳞状细胞癌间表达差异无统计学意义（P＞0.05）。结论   FOXP3+淋巴细胞在表皮良性肿瘤、癌前病变、恶性肿瘤中的表达呈逐渐增高趋势,在表皮肿瘤的免疫逃逸中发挥重要作用,促进了其发生发展。     

蕈样肉芽肿组织中psoriasin的表达及意义

目的研究psoriasin(S100A7)在蕈样肉芽肿中的表达及作用机制。方法用免疫组化方法检测正常皮肤组织及蕈样肉芽肿皮损中psoriasin蛋白的表达变化。结果 psoriasin蛋白不表达于正常皮肤组织;在21例蕈样肉芽肿中,可见psoriasin蛋白表达于角质层及棘层,而不表达于基底层,阳性率为90.5%。在蕈样肉芽肿中,psoriasin的表达明显低于正常皮肤组织(P<0.001)。结论 psoriasin可能通过趋化CD4+T细胞在蕈样肉芽肿的发病中起作用。     

窄谱中波紫外线治疗多种皮肤病疗效观察

紫外线照射治疗皮肤病效果明确，我们用窄谱中波紫外线（NB-UVB）照射治疗银屑病、玫瑰糠疹、白癜风、带状疱疹后遗神经痛、蕈样肉芽肿疗效显著，现报告如下。     

皮肤淋巴细胞增生性疾病患者发生人疱疹病毒8感染

目的:调查皮肤淋巴细胞增生性疾病患者犤如大斑块型副银屑病(LPP)和蕈样肉芽肿犦发生人疱疹病毒8(H H V-8,K aposi肉瘤相关疱疹病毒)感染的比率,并与炎性皮肤病或健康对照者作比较。设计:对123例有不同临床疾病的研究对象进行调查。机构:意大利罗马San Gallicano皮肤病协会皮肤     

CD4、CD8和γ受体在色素性紫癜性皮肤病T细胞中的表达

目的:根据T细胞表面抗原CD4、CD8和受体γ的表达分析色素性紫癜性皮肤病(Pigmented purpuric dermatoses,PPD)和蕈样肉芽肿(Mycosis fungoides,MF)的关系,探讨前者能否被视为一种皮肤淋巴样疾病(Cutaneons lymphoid dyscrasia,CLD).方法:免疫组化技术检测23例确诊PPD患者皮损表皮和真皮T淋巴细胞表面抗原CD4、CD8的表达情况,PCR测定T细胞受体γ(T cell receptor γ,TCRγ)单克隆基因重排.设立20例MF皮损、20例正常人皮肤组织为对照.结果:23例PPD中的15例有淋巴细胞向表皮的明显迁移,其中的5例和20例MF皮损可见淋巴细胞亲表皮现象,正常组未见此现象;18例PPD、20例MF和6例正常皮肤免疫染色以CD4+T为主;PPD和MF两组在真皮中的CD4+T数分别为38.2±30.6和68.8±47.6(P<0.017),CD4+/CD8+值分别为1.7±1.1和2.6±0.8(P<0.05),PPD组数值小于MF组,有显著差异;其余表皮和真皮中两组间的CD4+T、CD8+T计数和CD4+/CD8+值均较接近.TCRγ的PCR扩增结果显示23例PPD中1O例阳性,20例MF中16例阳性.正常组无1例阳性.其中7例阳性PPD标本见淋巴细胞向表皮的明显迁移(包括3例有亲表皮现象的PPD),8例以CD4+T细胞为主,9例表现为除双小腿以外的其它部位也存在皮疹.结论:PPD在T细胞表面抗原和受体的表达上有部分类似蕈样肉芽肿的特征,可能系一种皮肤淋巴样疾病.对于皮损分布广泛,治疗效果欠佳,免疫表型和分子学特征类似蕈样肉芽肿的病例在诊疗上应尤为重视,加强随访.     

表现为环状红斑的蕈样肉芽肿病

背景:蕈样肉芽肿病是一种淋巴瘤,典型的临床表现可包含红斑鳞屑样皮损,临床还可有多种不典型的表现,包括色素过度沉着或色素减退、暗示坏疽性脓皮病或瘙痒证。作者报道1例蕈样肉芽肿病病例,罕见的是其离心性环状红斑表现。方法:1例78岁男性有20多年的副银屑病的斑块样表现。2002-05因斑块的浸润及无瘙痒状况求诊。临床检查示1例窝、右腹股沟、左面颊处3块离心性红斑皮损。组织学显示Pautrier微脓肿与蕈样肉芽肿病诊断相符。局部激素和Chlorom ethin治疗后面部斑块自行消退,其他2块减轻变淡。然而,尽管继续治疗并合并PU VA治疗,仍有新斑块…     

红斑及红斑鳞屑性皮肤病

红斑及红斑鳞屑性皮肤病是以红斑及红斑鳞屑为主要特征的皮肤病,是皮肤科常见的一类疾病。临床上常见的此类疾病有:多形性红斑、银屑病、玫瑰糠疹、扁平苔藓、毛发红糠疹、线状苔藓等,其中以前三种病最为常见。1多形性红斑俗称猫眼疮,是一种以多形性皮疹和虹膜样(猫眼样、靶样)红斑为特征的自限性炎症性皮肤病。其发病原因以单纯疱疹病毒感染,巴比妥类、磺胺类、水杨酸盐类药物以及某些系统性疾病有关。该病多发于春秋季节,临床上分为红斑-丘疹型、水疱-大疱型、重症型,以红斑-丘疹型最为常见,好发于四肢远端、面颊及耳廓,皮损为水肿性红斑,…     

蕈样肉芽肿皮损中DNA含量及细胞增殖活性的检测

目的:研究蕈样肉芽肿(MF)皮损中DNA含量、细胞周期分布与分期的关系,以探讨DNA倍体及细胞增殖活性在该病早期诊断、治疗及预后判断方面的作用。方法:应用流式细胞仪检测38例蕈样肉芽肿皮肤损害石蜡包埋组织中的DNA含量及细胞周期分布。结果:38例蕈样肉芽肿皮损石蜡包埋组织中异倍体率、S期细胞比率(SPF)及细胞增殖指数(PI)随病情的进展而增高。红斑期与斑块期、斑块期与肿瘤期的SPF值及红斑期与斑块期PI值之间有统计学差异(P<0.001);斑块期与肿瘤期PI值之间无统计学差异(P>0.05)。结论;蕈样肉芽肿皮损组织中的DNA倍体及细胞增殖活性有可能成为该病临床诊断及预后判断的重要因素。     

副银屑病的诊治

副银屑病是一种较为少见的皮肤病,以红斑、丘疹、浸润、脱屑而无自觉症状或轻微瘙痒为其特征。1诊断要点①发病部位:全身均可发病。②皮疹特点:红斑、丘疹、浸润和鳞屑,形态表现不一。③自觉症状:缺如或仅有轻微瘙痒。④病程呈慢性经过。⑤病理为非特异性表现。⑥须排除其他几种疾病:银屑病,玫瑰糠疹、扁平苔藓、蕈样肉芽肿、血管萎缩性皮肤异色症及丘疹坏死性结核疹等病。2治疗①抗组胺剂口服,第1代和第2代抗组胺剂均可。②维生素类:可应用维生素C、P和D2。③可给予硫代硫酸钠静脉注射。④使用砷剂、氨苯砜。⑤应用组织疗法、封闭疗法。…     

红斑鳞屑性皮肤病患者静脉留置针固定方法的改进

红斑鳞屑性皮肤病包括寻常型银屑病、关节病型银屑病、脓疱型银屑病、红皮病、玫瑰糠疹、扁平苔藓、多形红斑等疾病。皮肤表现分别以皮损、皮疹、脱屑、脓疱为主，可累及皮肤任何部位。由于经斑鳞屑性皮肤病患者特殊的脱屑、皮损表现，对静脉输液固定产生一定的影响，使静脉留置针脱出很常见，静脉留置针最多不超过3天就需重新置管。我科尝试了一种新的简易静脉留置针的固定方法，取得满意效果，现报告如下：     

200例玫瑰糠疹的临床特征及其与人类疱疹毒6感染的关系

目的分析玫瑰糠疹的临床特征及其与人类疱疹病毒6感染的关系,旨在提高对该病的诊疗水平。方法对200例玫瑰糠疹患者的临床资料进行回顾性分析,总结其发病特点;同时采用PCR法对其中44例急性期患者的血浆、外周血单核细胞(PBMC)、皮损、唾液标本中人类疱疹病毒6(HHV-6)的DNA进行检测。结果经对200例患者发病的分季统计显示,春、夏、秋季间玫瑰糠疹发病率无显著差异(均P>0.05),对皮损特点、分布情况和自觉症状统计发现存在不典型的皮损、不典型的分布部位与自觉症状的患者。此外,经PCR检测证实44例急性期患者中部分存在HHV-6感染。结论除春秋季外,夏季也是玫瑰糠疹的高发季节;其可表现为不典型的皮损、不典型的发病部位及伴有显著瘙痒,值得临床医师重视;其发病可能与HHV-6感染有关。     

Expression of Apoptosis Related Protein in Skin Lesions of Lichen Planus and Its Implication

In order to investigate the role of Caspase-3 and Bax in the pathogenesis of lichen planus, immunohistochemistry was used to detect the expression of Caspase-3 and Bax in skin lesions of the patients with lichen planus and skin tissues of normal subjects. The results showed that positive rate of Caspase-3 and Bax expression in lichen planus were significantly higher than that in normal skins （both P〈0.05）. Meanwhile, there was a obvious correlation between the increase of Caspase-3 and that of Bax in lichen planus. The expression of Caspase-3 and Bax might play an important role in the development of lichen planus.     

The Expression of TNF-α and ICAM-1 in Lesions of Lichen Planus and Its Implication

In order to investigate the role of TNF-α and ICAM-1 in the pathogenesis of lichen planus, immunohistochemistry was used to detect the expression of TNF-α and ICAM-1 in skin le- sions of the patients with lichen planus and skin tissues of normal subjects. The results showed that positive rates of TNF-α and ICAM-1 expressions in lichen planus were significantly higher than those in normal skins (both P<0.05). Meanwhile, there was a obvious correlation between the in- crease of TNF-α and that of ICAM-1 in lichen planus. The expression of TNF-α and ICAM-1 might play an important role in the development of lichen planus.     

扁平苔藓皮损中MIF、MMP-9的表达及意义

目的：探讨巨噬细胞移动抑制因子（MIF）与基质金属蛋白酶9（MMP-9）在扁平苔藓发病中的作用。方法：采用免疫组化方法和RT-PCR法检测30例扁平苔藓组织和30例正常皮肤组织中MIF与MMP-9的表达情况。结果：MIF与MMP-9在扁平苔藓组织中的表达明显高于正常皮肤组织,差异有统计学意义（P〈0.05）;MIF和MMP-9在扁平苔藓皮损中的表达呈正相关（r=0.636,P〈0.01）。结论：MIF在扁平苔藓基底膜带损坏过程中可能有促进作用,其机制可能与上调MMP-9的表达有关。     

The expression of TNF-α and ICAM-1 in lesions of lichen planus and its implication

Summary In order to investigate the role of TNF-and ICAM-1 in the pathogenesis of lichen planus, immunohistochemistry was used to detect the expression of TNF-and ICAM-1 in skin lesions of the patients with lichen planus and skin tissues of normal subjects. The results showed that positive rates of TNF-α and ICAM-1 expressions in lichen planus were significantly higher than those in normal skins (both P<0.05). Meanwhile, there was a obvious correlation between the increase of TNF-α and that of ICAM-1 in lichen planus. The expression of TNF-α and ICAM-1 might play an important role in the development of lichen planus.     

扁平苔藓中早期生长反应因子-1和肿瘤坏死因子-α的表达及意义

目的：探讨早期生长反应因子-1（Egr-1）和肿瘤坏死因子-α（TNF-α）在扁平苔藓发病中的作用。方法：采用TUNEL原位凋亡检测法检测30例扁平苔藓皮损及30例正常皮肤组织中细胞凋亡情况,并用免疫组织化学法检测该30例扁平苔藓和30例正常皮肤组织中Egr-1和TNF-α的表达情况。结果：扁平苔藓皮损处角质形成细胞和真皮浅层浸润的淋巴细胞中细胞凋亡水平明显高于对照组;在相同部位,Egr-1和TNF-α的表达水平高于正常皮肤组织,差异有统计学意义（P〈0.05）,且二者在扁平苔藓皮损处的表达呈正相关（r=0.896 7,P〈0.05）。结论 ：Egr-1和TNF-α的高表达可能参与了扁平苔藓的发病。     

Expression of TLR9 and Its mRNA in the Lesions of Lichen Planus

To investigate the role of toll-like receptor 9 (TLR9) in the pathogenesis of lichen planus, the expressions of TLR9 and its mRNA in the lesional skin of lichen planus were detected by immu- nohistochemical technique (SP) and RT-PCR. As control, normal skin of healthy volunteers was also tested. The immunohistochemical study showed that the expression of TLR9 in the lesional skin of lichen planus was significantly higher than that in the normal controls. The results of RT-PCR showed that both skin lesions and normal controls had TLR9 expression. In skin lesions, the expression level of TLR9 mRNA was 1.6075±0.0930, which was significantly higher than that in normal controls (P<0.001). These findings indicated that up-regulated expression of TLR9 and its mRNA might be involved in the pathogenesis of lichen planus.     

蕈样肉芽肿患者HLA-DR、LFA-1、ICAM-1、CD4和CD8抗原的表达

目的:探讨蕈样肉芽肿(MF)患者外周血淋巴细胞和皮损中人白细胞DR抗原(HLA-DR)、淋巴细胞功能相关抗原1(LFA-1)、细胞间黏附分子1(ICAM-1)、分化群抗原4、8(CD4、CD8)抗原表达及其意义。方法:应用流式细胞仪和免疫组化技术检测15例MF患者的外周血和皮损HLA-DR、LFA-1、ICAM-1、CD4、CD8抗原的表达并与正常人相比较。结果:MF患者外周血HLA-DR 、LFA-1 及ICAM-1 淋巴细胞比正常对照增高(P均<0.01);MF患者外周血CD4 淋巴细胞较正常对照下降(P<0.001);MF患者皮损中HLA-DR 细胞、CD4 细胞、CD8 细胞及LFA-1 、ICAM-1 细胞的表达均比正常对照增高。结论:MF患者外周血淋巴细胞和皮损HLA-DR和LFA-1、ICAM-1、CD4、CD8抗原表达普遍存在异常,与MF的发病密切相关。     

The Expression of TNF-α and ICAM-1 in Lesions of Lichen Planus and Its Implication

In order to investigate the role of TNF-α and ICAM-1 in the pathogenesis of lichen planus, immunohistechemistty was used to detect the expression of TNF-α and ICAM-1 in skin le- sions of the patients with lichen planus and skin tissues of normal subjects. The results showed that positive rates of TNF-α and ICAM-1 expressions in lichen planus were significantly higher than those in normal skins (both P<0.05). Meanwhile, there was a obvious correlation between the in- crease of TNF-α and that of ICAM-1 in lichen planus. The expression of TNF-α and ICAM-1 might play an important role in the development of lichen planus.