金雀异黄素对肾肿瘤细胞系GRC-1侵袭能力的影响

目的 研究酪氨酸激酶抑制剂用于肾肿瘤转移抑制的可能性。方法 用免疫组织化学、Zymography、Transwell小室等方法检测肾肿瘤细胞系GRC—1在酪氨酸激酶抑制剂金雀异黄素的作用下侵袭能力的改变。结果 金雀异黄素可降低GRC—1细胞的侵袭能力，抑制其基质金属蛋白酶(MMP)的分泌，改变细胞骨架中波形蛋白(vimentin)的排列方式。结论 金雀异黄素可明显降低肾肿瘤细胞的侵袭能力，其机理可能是通过抑制基质金属蛋白酶的分泌和通过干扰肿瘤细胞骨架中波形蛋白的方式抑制细胞运动。     

金雀异黄素对人乳腺癌细胞体外增殖作用机制的研究

目的研究金雀异黄素（Genistein,Gen）对人乳腺癌细胞株MCF-7凋亡的影响及凋亡相关基因表达的调控。方法选用人乳腺癌细胞株MCF-7体外培养,MTT法检测Gen对乳腺癌生长活力的影响,流式细胞仪检测细胞周期及凋亡率,Giemsa染色观察细胞形态学改变,免疫组化检测凋亡相关基因Bax和癌基因erbB-2蛋白表达水平的改变。结果金雀异黄素对MCF-7细胞体外生长具有明显的抑制作用。Giemsa染色显示,金雀异黄素处理的MCF-7细胞呈明显的凋亡形态改变,细胞固缩、发泡,染色质凝集呈块状,并沿核周分布。流式细胞仪检测在G。峰前可见凋亡峰,且随用药时间的延长,凋亡比率递增。蛋白水平检测表明金雀异黄素促进MCF-7细胞Bax表达升高,抑制erbB-2蛋白的表达。结论金雀异黄素通过诱导细胞凋亡或阻滞细胞于Gz～M期,从而抑制乳腺癌细胞的体外增殖,并主要是通过调节Bax和erbB-2蛋白的表达实现的。     

Src激酶抑制剂-1对人软骨肉瘤细胞生物学行为的影响

目的探讨Src激酶抑制剂-1对人软骨肉瘤SWl353细胞生长、凋亡和迁移等生物学行为的影响及其机制。方法体外培养人软骨肉瘤SWl353细胞，分为对照组和实验组，分别采用MTT法、流式细胞术和细胞迁移实验观察Src激酶抑制剂-1对人软骨肉瘤SWl353细胞生长、凋亡和迁移等生物学行为的影响，并通过Western—blot检测Src激酶抑制剂-1作用后Src及下游信号通路蛋白的影响。结果0．5、1、5、10μmol／L的Src激酶抑制剂-1均能明显抑制SWl353细胞的增殖（P〈0．01）；1、5、10μmol／L的Src激酶抑制剂-1均能明显促进SWl353细胞的凋亡（P〈0．01）；Sre激酶抑制剂-1能明显限制SWl353细胞的迁移；Sre激酶抑制剂-1能明显抑制SWl353细胞Src（Tyr416）及下游信号ERK，bAkt和FAK（F397）的磷酸化。结论Src激酶抑制剂-1具有抑制人软骨肉瘤SWl353细胞生长和迁移的能力，可能对软骨肉瘤具有一定的治疗效果，这是通过直接抑制Src酪氨酸激酶的活性，进而抑制其下游信号通路的激活来实现的。     

人骨形成蛋白2反义RNA对骨肉瘤细胞OS-9901生物学活性的影响

探讨骨形成蛋白（BMP）对人骨肉瘤细胞的作用及意义．方法：将重组人BMPZ基因的反义核酸逆转录病毒表达载体PDOR－AB用脂质体导入BMP表达阳性的骨肉瘤细胞OS－99OI，观察骨肉瘤细胞OS－99OI的BMP表达、增殖活性、肿瘤抑制基因＂m23的表达、细胞周期和电镜下形态改变．结果：转染后，OS－99o1细胞中内源性的BMP表达产物明显下降；增殖活性明显下降；肿瘤转移抑制基因＂m23表达未见改变；细胞周期分析表明：GI期细胞比例上升，S期比例下降；电镜观察可见细胞质中溶酶体增多，染色质有断裂现象．结论：反义核酸技术阻断BMPZ在OS－99o1细胞中的表达后，肿瘤细胞的增殖活性降低，证实骨肉瘤细胞中BMP的表达增加了肿瘤细胞的增殖活性．     

重组人生长激素对肾癌细胞株细胞周期动力学影响

目的　本实验试图阐明生长激素对于肾癌细胞有无促增殖和分化作用。方法　取对数生长期的人肾癌细胞株GRC -1,以氟尿嘧啶和 /或不同浓度的重组人生长激素 (rhGH)体外培养 ,2 4小时以后流式细胞仪测定细胞增殖周期和细胞凋亡等指标。结果　在空白对照组 ,G0 ～G1期的细胞数约占 5 3%,S期细胞数次之 ,G2 ～M期占不足 4%。在生长激素组 ,G0 ～G1期细胞数率降低 (P <0 .0 5 ) ,S期细胞百分率增高 (P <0 .0 1)。在单纯氟尿嘧啶组 ,G0 ～G1期细胞数增高 ,S期细胞数降低 ;同时加氟尿嘧啶和生长激素组与单纯氟尿嘧啶组相比 ,G0 ～G1期细胞数进一步增加 ,S期细胞数进一步降。结论　rhGH在体外作用于GRC -1细胞 ,能诱导细胞分化 ,促使静止细胞群进入增殖周期 ,并使处于DNA合成期的细胞增多 ,说明rhGH在体外有促进肾癌细胞生长增殖的作用 ,所以 ,我们推断肾癌细胞膜表面可能存在生长激素受体。氟尿嘧啶能使S期细胞数降低即抑制细胞的增殖 ,与氟尿嘧啶的作用机理相符。生长激素和氟尿嘧啶合用 ,S期细胞数进一步降低 ,说明生长激素能增强氟尿嘧啶抗肿瘤细胞生长增殖作用。     

EGF受体特异的酪氨酸激酶抑制剂对早期鼠胚体外发育的影响

将表皮生长因子（ＥＧＦ）受体特异的酪氨酸激酶抑制剂与２－细胞鼠胚联合体外培养２４，４８和６０ｈ。结果显示：受抑制剂作用的胚胎，５５．２％发育不超过个细胞期，仅１．５％的胚胎能发育形成胚泡，表明抑制剂能显著影响４－细胞和桑椹胚的进一步发育。提示ＥＧＦ受体在早期鼠胚发育过程中具有增殖和分化双重性作用。     

金雀异黄素下调Gli1表达抑制MHCC97H细胞系肝癌干细胞样细胞侵袭

目的:研究金雀异黄素能否和如何抑制肝细胞癌MHCC97H细胞系肝癌干细胞样细胞侵袭。方法:干细胞条件培养基悬浮培养MHCC97H细胞系球形成细胞,作为肝癌干细胞样细胞。不同浓度金雀异黄素处理后,Transwell小室侵袭试验检测体外细胞侵袭能力;Western blot分析Gli1和Snail1蛋白表达。结果:干细胞条件培养基悬浮培养MHCC97H细胞能形成肿瘤球。金雀异黄素(5、10和20μM)作用第3代球形成细胞即肝癌干细胞样细胞72 h降低肿瘤球形成率和细胞侵袭率;呈浓度依赖性。金雀异黄素(5、10和20μM)孵育肝癌干细胞样细胞24 h下调Gli1和Snail1蛋白表达。结论:金雀异黄素可能通过调控Gli1抑制Snail1蛋白表达抑制肝癌干细胞样细胞侵袭。     

人参皂苷对人体骨肉瘤细胞U2OS增压的影响

目的：为寻找人参中抑制骨肉瘤细胞增殖的活性成分,方法：采用细胞计数法检测了27种从人参中得到的单体化合物对体外培养的人体骨肉瘤细胞U2OS增殖的影响。以流式细胞术分析DNA含量, 抑制肿瘤细胞增殖作用的化合物对肿瘤细胞的细胞增殖周期的变化进行研究,另外,以荧光染色法检测了人掺皂苷对细胞死亡率变化的影响。结果对骨肉细胞增殖的抑制作用研究表明,在5μmol/L浓度下,人参皂苷－Ro-Rh1,-Rh2,F1和－L8较明显地抑制了肿瘤细胞的增殖,人参皂苷－Rg1,F3,Rf,PPT和PT显著地抑制了肿瘤细胞的增殖,进一步对骨肉瘤细胞有抑制作用的化合物检测其对细胞增殖周期影响发现;人参皂苷－Ro,-Rf,-Rg1,-F1-Rh2,PPT和PT使处于G0／G1期的细胞数目明显增多,伴随着S期和G2＋M期的细胞明显减少,说明这些化合物抑制了肿瘤细胞增殖周期的进行,与对照相比人参皂苷－Rf1-,Rg1-F1和PPT显著地促进了肿瘤细胞的细胞死亡现象。结论人参皂苷是通过阻止细胞增殖周期的G0／G1期或促使细胞死亡两种方式抑制了肿瘤细胞U2OS的增殖。     

植物雌激素对乳腺癌的抑制作用

目的 研究金雀黄素抑制人乳腺癌细胞株体外增殖作用及其作用机理.方法 选用两种不同的人乳腺癌细胞株MCF-7和MDA-MB-231体外培养,MTT法检测细胞增殖作用,Giemsa染色观察细胞形态学改变,流式细胞仪检测细胞周期及凋亡率,观察不同剂量的金雀黄素诱导细胞凋亡.同时采用免疫组化检测凋亡相关基因Bax和癌基因erbB-2蛋白的表达.结果 金雀黄素对MCF-7细胞和MDA-MB-231细胞体外生长具有明显的抑制作用.Giemsa染色显示,金雀黄素处理的MCF-7细胞呈明显的凋亡形态改变,细胞固缩、发泡,染色质凝集呈块状,并沿核周分布.流式细胞仪检测在G1峰前可见凋亡峰,且随用药时间的延长,凋亡比率递增.而金雀黄素处理的MDA-MB-231细胞未见明显的凋亡形态改变和凋亡峰的出现,细胞周期明显地阻滞于G2～M期.蛋白水平检测表明金雀黄素促进MCF-7细胞Bax表达升高,抑制erbB-2蛋白的表达.结论 金雀黄素通过诱导细胞凋亡或阻滞细胞于G2～M期,从而抑制乳腺癌细胞的体外增殖,并主要是通过调节Bax和erbB-2蛋白的表达实现的,为其减缓人乳腺癌细胞的体内生长提供理论依据.     

金雀异黄素抑制肝癌干细胞样细胞的特性及对氟尿嘧啶耐药性的影响

目的：研究金雀异黄素对SMMC-7721细胞系肝癌干细胞样细胞特性以及氟尿嘧啶敏感性的影响。方法不同浓度金雀异黄素处理SMMC-7721细胞系球形成细胞。肿瘤球形成法检测自我更新能力。MTT比色法测定氟尿嘧啶药物敏感性。Western blot分析干细胞标志物CD133、CD44、Gli1和ABCG2蛋白表达。结果金雀异黄素显著抑制SMMC-7721细胞肿瘤球形成,并优先抑制球形成细胞增殖活性(P<0.05)。金雀异黄素(10μmol/L)有效逆转球形成细胞对氟尿嘧啶的耐药性。金雀异黄素降低CD133、CD44、Gli1和ABCG2蛋白表达水平。结论金雀异黄素抑制肝癌干细胞样细胞特性和氟尿嘧啶耐药性,其作用机制与抑制干细胞标志物蛋白表达和阻断Hedgehog信号传导有关。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.