雷公藤多甙对哮喘豚鼠炎症的抑制作用

目的：通过哮喘豚鼠模型研究雷公藤多甙（ｔｒｉｐｔｅｒｙｇｉｕｍｐｏｌｙｇｌｙｃｏｓｉｄｅ，ＴⅡ）对哮喘豚鼠炎症的抑制作用。方法：用卵白蛋白诱发致敏豚鼠哮喘发作，同时进行ＴⅡ与地塞米松（ｄｅｘａｍｅｔｈａｓｏｎｅ，ＤＥＸ）的对照治疗，观察豚鼠支气管－肺泡灌洗液（ＢＡＬＦ）中细胞学、肺组织病理及超微结构的变化。结果：豚鼠诱发哮喘后２４ｈ和７２ｈＢＡＬＦ中炎性细胞、嗜酸细胞总数及其分类计数的结果显示，哮喘组较正常对照组明显增多，而ＴⅡ和ＤＥＸ治疗组尤其是ＴⅡ大剂量组则明显减少，与正常对照组比无显著差异（Ｐ＞０．０５），与哮喘组比有明显差异（Ｐ＜０．０５）。组织形态学也显示ＴⅡ对哮喘豚鼠迟发性哮喘反应有明显的抑制作用。结论：ＴⅡ对哮喘豚鼠炎症有明显的抑制作用。     

地塞米松对哮喘豚鼠肺表面活性物质超微结构的影响

目的观察地塞米松对哮喘豚鼠肺表面活性物质系统(PS)超微结构的影响。方法18只雄性豚鼠随机分为3组,哮喘组豚鼠采用卵蛋白(OVA)致敏加雾化吸入激发的方法制成哮喘模型;地塞米松干预组豚鼠致敏和激发的方法同哮喘组,在第二次激发前给予腹腔注射地塞米松;正常对照组豚鼠用生理盐水腹腔注射加生理盐水雾化吸入。OVA激发结束后行肺组织固定,电子显微镜观察各组PS超微结构的变化。结果对照组PS层结构连续,肺泡Ⅱ型上皮细胞内含丰富板层小体。哮喘组PS层丧失正常的连续结构,肺泡Ⅱ型上皮细胞内板层体空泡化。地塞米松干预组PS层基本连续,肺泡Ⅱ型上皮细胞内板层体少量空泡化。结论哮喘豚鼠超微结构出现异常改变,应用地塞米松治疗后PS超微结构异常改变减轻。     

三氧化二砷对哮喘豚鼠肺内核因子-κB表达的影响

目的：研究Ａｓ２Ｏ３对哮喘豚鼠肺内核因子－κＢ（ｎｕｃｌｅａｒｆａｃｔｏｒ－ｋａｐｐａＢ，ＮＦ－κＢ）表达的影响及其作用机制。方法：豚鼠３０只随机分为３组，即对照组、哮喘组和Ａｓ２Ｏ３（２ｍｇ／ｋｇ）治疗组。采用免疫组织化学染色技术，即小鼠抗大鼠ｐ６５单克隆抗体（Ｆ－６）、生物素化马抗小鼠ＩｇＧ、二氨基联苯胺（ＤＡＢ）显色检测豚鼠气道壁ＮＦ－κＢ；计算机图像分析技术对气道壁ＮＦ－κＢ表达进行定量检测。结果：对照组豚鼠整个肺组织包括气管、支气管、肺泡和脏层胸膜构成了一个ＮＦ－κＢ阳性（ＮＦ－κＢ＋）细胞网络，其中支气管壁胞核ＮＦ－κＢ＋细胞密度为（２２０±７２）／ｍｍ２上皮面积；与对照组相比，哮喘组豚鼠气道壁胞核ＮＦ－κＢ＋细胞密度显著增加（Ｐ＜０．０１）；哮喘豚鼠经Ａｓ２Ｏ３处理后，气道壁胞核ＮＦ－κＢ＋细胞密度显著下降（Ｐ＜０．０１），但与对照组相比仍较高（Ｐ０．０１８）。结论：ＮＦ－κＢ激活增加是支气管哮喘重要的发病机制之一；下调肺内ＮＦ－κＢ激活细胞数量可能是Ａｓ２Ｏ３治疗哮喘的一个重要机制。     

氨茶碱雾化吸入对正常及哮喘豚鼠气道粘膜的影响

目的 观察吸入氨茶碱对正常豚鼠气道粘膜的影响及对豚鼠哮喘的预防作用。方法 ３４只豚鼠随机分为６组：对照组、正常动物氨茶碱吸入组、哮喘组、哮喘豚鼠０．５％氨茶碱吸入组、哮喘豚鼠地塞米松吸入组、哮豚鼠０．５％氨茶碱＋地塞米松吸入组,分别行肺泡灌洗液（ＢＡＬＦ）、血清茶碱浓度及病理学检验。结果 正常动物吸入氨茶碱后气道粘膜上皮纤毛不完整,上皮细胞脱落,表面有炎性渗出,粘膜及粘膜下层炎性细胞浸润明显,肺泡     

哮喘对豚鼠肺表面活性物质的影响

肺表面活性物质（ｐｕｌｍｏｎａｒｙｓｕｒｆａｃｔａｎｔ,ＰＳ）对于肺发挥正常呼吸功能有着重要的生理作用。已证实哮喘发作可能也与ＰＳ相对不足有关［１］,为此,我们从动物支气管肺灌洗液（ＢＡＬ）等方面论证了ＰＳ与哮喘的关系,以探讨ＰＳ在哮喘发病机制中的作用。一、材料和方法１动物分组：雄性豚鼠６０只（２５０～５００ｇ）,用随机数字表法将其随机分为对照组和哮喘组,每组３０只。每组再分肺病理形态观察组、ＢＡＬ中磷脂和蛋白定量测定组、ＢＡＬ表面活性测定组及支气管肺灌流组,每个亚组６只豚鼠。２模型建立：以…     

钙ATP酶在豚鼠过敏性哮喘发病过程中的作用

本实验给致敏豚鼠吸入卵蛋白建立速发型过敏性哮喘的动物模型，观察了呼吸系总阻抗（Ｚｒｓ）及豚鼠肺组织钙ＡＴＰ酶（Ｃａ２＋－ＡＴＰａｓｅ）活性的变化以及６５４－２，异搏定对二者的影响。结果表明哮喘组Ｚｒｓ明显高于正常组（Ｐ＜０．０１），肺组织Ｃａ２＋－ＡＴＰａｓｅ活性明显低于正常组（Ｐ＜０．０１），６５４－２有增Ｃａ２＋－ＡＴＰａｓｅ活性的作用。说明在过敏性哮喘的发病过程中Ｃａ２＋－ＡＴＰａｓｅ活性下降是其病理生理变化的一个重要因素，细胞内钙浓度升高可能与此有关。     

雷公藤多甙对哮喘豚鼠炎症的抑制作用

目的：通过哮喘豚鼠模型研究雷公藤多甙对哮喘豚鼠类症的抑制作用。方法：用卵白蛋白诱发致敏豚鼠哮喘发作，同时进行ＴⅡ与地塞米松（ＤＥＸ）的对照治疗，观察豚鼠支气管－肺泡灌洗液（ＢＡＬＦ）中细胞学、肺组织病理及超微结构的变化。结果：豚鼠诱发哮喘后２４ｈ和７２ｈＢＡＬＦ中炎性细胞、嗜酸细胞总数及其分类计数的结果显示，哮喘组较正常对照组明显增多，而ＴⅡ和ＤＥＸ治疗组尤其是ＴⅡ大剂量组则明显减少，与正常对照组     

哮喘气道炎症粘附机制的实验研究

目的：评价细胞间粘附分子１（ＩＣＡＭ－１）、Ｐ选择素在哮喘气道炎症粘附机制中的作用,进一步阐明哮喘的发病机理。方法：用酶联免疫吸附试验、肺组织免疫组化检查和呼吸生理学方法系统观察正常组和哮喘组豚鼠各项指标。结果：（１）哮喘组豚鼠肺潮气量、动态肺顺应性（Ｃｄｙｎ）和肺气道阻力与对照组比较差异有显著性（Ｐ＜０．０１及Ｐ＜０．０５）。（２）哮喘组豚鼠血浆和肺泡灌洗液（ＢＡＬＦ）可溶性ＩＣＡＭ－１（ｓＩＣＡＭ－１）、可溶性Ｐ选择素、血清和ＢＡＬＦ嗜酸粒细胞阳离子蛋白（ＥＣＰ）与对照组比较,差异有显著性（Ｐ＜０．０１）;ＢＡＬＦ中白细胞介素８（ＩＬ－８）与对照组比较差异也有显著性（Ｐ＜０．０１）。（３）哮喘组豚鼠肺组织（气道上皮和血管内皮）ＩＣＡＭ－１和ＩＬ－８表达与对照组比较,差异有显著性（Ｐ＜０．０１）。结论：ＩＣＡＭ－１、Ｐ选择素、ＩＬ－８、ＥＣＰ参与介导了哮喘气道炎症的粘附过程。     

雷公藤对哮喘豚鼠嗜酸性细胞凋亡及IL-5和GM-CSF mRNA表达的研究

目的：探讨雷公藤对哮喘豚鼠酸性细胞（Ｅｏｓｉｎｏｐｈｉｌ,Ｅｏｓ）凋亡及与白介素－５（ＩＬ－５）,粒细胞／巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）ｍＲＮＡ表达的作用。方法：实验豚鼠分为哮喘组,雷公藤治疗组和下沉对照组,采用原位细胞凋亡（ＴＵＮＥＬ）和原位杂交技术分别检测豚鼠支气管肺泡灌洗涤中的Ｅｏｓ的凋亡率和肺组织ＩＬ－５,ＧＭ－ＣＳＦ ｍＲＮＡ的表达强度均显著高于处理和对照组,哮喘组ＢＡＬＦ中Ｅｏｓ     

低压缺氧治疗哮喘后血浆皮质醇BALF中嗜酸性粒细胞的变化

目的：探讨低压缺氧治疗哮喘的机制,方法：采用卵蛋白致敏和激发制成豚鼠哮喘模型,比较正常组,诱发哮喘发作后２４小时（发作组）,未经任何治疗哮喘豚鼠（对照组）以及低压缺氧治疗哮喘豚鼠（治疗组）的血浆皮质醇、支气管肺泡灌洗液（ＢＡＬＦ）中嗜酸性粒细胞（ＥＯＳ）和低密度嗜酸性粒细胞（ＨＥＯ）以及肺组织病理学变化。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.