在创伤愈合中VEGF和Ang-1表达的生物动力学变化研究

目的研究大鼠皮肤损伤愈合过程中血管内皮细胞生长因子（VEGF）和促血管生成素-1（Ang-1）表达的动态变化，阐明其在组织修复血管再生中的作用。方法在大鼠背侧切开皮肤全层建立大鼠创伤模型，不同时问后活体取材，应用HE染色观察组织病理形态学变化；应用免疫组化SP法检测VEGF和Ang-1的蛋白表达。结果伤口组织病理学检查可见，毛细血管在伤后第3d即见增多，伤后第7d达高峰，随后逐渐减少。血管内皮细胞胞浆VEGF蛋白表达在伤后第1d呈阳性。伤后第3d呈强阳性，伤后第5d呈阳性，而于伤后第7d阳性程度逐渐减弱。伤后第1～14d VEGF蛋白表达与对照组比较，有非常显著性差异（P〈0．01）。血管内皮细胞胞浆Ang-1蛋白表达在伤后第1d部分呈阳性表达，部分呈弱阳性表达，在伤后第3d呈阳性表达。伤后第5、7d呈强阳性、阳性表达。随后阳性程度逐渐减弱。伤后第3～21d Ang-1蛋白表达与对照组比较，有非常显著的统计学差异（P〈0．01）。结论VEGF和Ang—1在血管内皮细胞中的表达强度与组织损伤愈合程度密切相关，在创伤愈合过程中互相协调、促进并调节血管肉芽组织的生长和成熟。     

2型糖尿病时血管内皮细胞的超微结构观察

目的观察2型糖尿病时大鼠胸主动脉内皮细胞的光镜及形态学变化，探讨其病变的发生机制。方法复制高糖、高脂血症动物模型，光镜及电镜下观察胸主动脉内皮细胞的病理变化。结果①高糖血症：光镜下见胸主动脉内皮细胞肿胀、肥胖，呈立方状，三五成群向腔面突出。内皮下纤维组织轻度增生。电镜下内皮细胞表面有破损及绒毛状突起，并见血浆蛋白附着物；细胞浆内有较正常明显增多的糖原颗粒，细胞浆内吞饮小泡增多。细胞核周池增宽。②高脂血症：光镜下见内皮细胞肿胀、肥胖，个别细胞向腔面呈出芽状生长。电镜下内皮细胞表面有指状突起，胞浆内见较大的吞饮泡，平滑肌细胞有向内膜下迁移的倾向。结论2型糖尿病的高糖及高脂可不同程度地损伤血管内皮细胞，是导致进一步血管病变的重要因素。     

补肾安胎冲剂对复发性自然流产小鼠蜕膜组织超微结构的影响

目的 观察补肾安胎冲剂对复发性自然流产（recurrent spontaneous abortion,RSA）小鼠蜕膜组织血管、细胞形态及血管内皮细胞超微结构的影响。方法 采用DBA/2×CBA小鼠,按照Clark经典方法复制RSA小鼠模型。按妊娠顺序将小鼠随机分为正常妊娠组,模型组,西药组,补肾安胎冲剂高、中、低剂量组,每组10只,分别进行药物干预。光镜下观察蜕膜组织的血管和细胞形态,电镜下观察血管内皮细胞超微结构。结果 光镜下见模型组血管数量减少,蜕膜细胞胞浆明显水肿,部分核消失,细胞核固缩;补肾安胎冲剂高剂量组血管丰富且管壁完整,由多个血管内皮细胞环绕而成,小部分蜕膜细胞水肿,细胞核基本无坏死;补肾安胎冲剂中、低剂量组血管数量少于补肾安胎冲剂高剂量组,血管内皮细胞排列不整齐,部分蜕膜细胞胞浆水肿,空泡变,少数细胞核消失。电镜下见模型组血管壁不完整,血管内皮细胞结构不完整,细胞核呈梭形,粗面内质网、核糖体减少,部分线粒体已破坏;补肾安胎冲剂高剂量组血管壁完整,血管内皮细胞结构完整,核膜完整;胞浆中可见丰富粗面内质网及核糖体,线粒体结构完整、无肿胀变性,染色质分布均匀;补肾安胎冲剂中、低剂量组血管壁完整,部分血管内皮细胞肿胀,核膜完整,胞浆中粗面内质网扩张,核糖体轻度减少,部分线粒体肿胀、空泡变。结论 RSA模型小鼠存在母胎界面血管生成的障碍,补肾安胎冲剂可以修复RSA小鼠蜕膜组织中破坏的细胞结构,改善血管生成和细胞器完整度,发挥安胎作用。     

脑挫裂伤修复过程中胶质细胞的形态变化

为了阐明大脑皮质局灶性挫伤后，局部胶质细胞的形态学变化，用２０只大鼠制做脑挫伤动物模型，不同时间取脑进行光，电镜观察。结果表明：伤后３－７ｄ时胶质细胞呈水肿或质膜破裂；伤灶周围胶质细胞逐渐增生，密度增高，１４－２８ｄ时伤灶处被胶质细胞和小血管填充，其中有大量巨噬细胞样质细胞。文 中类胶质细胞超微结构和功能进行了阐述。     

高速投射物所致流体扰动和创伤血清对培养血管内皮细胞的影响

利用体外模拟循环管路和远达效应动物模型，观察致伤瞬间流体扰动和创伤血清对培养的猪主动脉内皮细胞（ＶＥＣ）的作用特点，探讨这两种致伤因素在损伤ＶＥＣ过程中的关系。发现高速投射物所致流体扰动可使ＶＥＣ的结构和功能发生明显改变。表现为ＶＥＣ膜破裂或皱缩；局灶性胞浆疏松、水肿；胞浆内空泡增多，细胞器离散、数目减少。内皮细胞生成和释放的６－ｋｅｔｏ－ＰＧＦ１α明显降低，ＡＣＥ、ＥＴ和ｆⅧＲ：Ａｇ显著增加。猪发生远达效应后，其４、６ｈ血清也可使ＶＥＣ结构和功能发生类似改变，但没有扰动流体所致ＶＥＣ结构和功能的改变严重。提示高速投射物致伤机体瞬间，循环管路内剧烈扰动的流体已可造成远隔部位组织脏器微血管内皮的损伤，这可能为远达效应的发生创造了条件。创伤后神经体液因子的改变则促使这种损伤进一步加剧，并为创伤后某些严重并发症的发生发展提供了病理形态学和病理生理学基础     

缺氧时单个肺动脉内皮细胞胞浆游离钙的动态变化

本室已测得缺氧使悬液状态肺动脉内皮细胞胞浆游离钙增加。而在体血管内皮细胞是以单层形式贴于血管内壁,故本实验进一步用双波长显微荧光测量系统观察单个贴壁肺动脉内皮细胞[Ca~(2 )]i的动态变化。 1 材料与方法 1.1 肺动脉内皮细胞分离、培养及鉴定:猪肺动脉内皮细胞(PAEC)培养用胰酶消化法。倒置显微镜见细胞形态呈鹅卵石样,单层融合;间接免疫荧光示胞浆中存在Ⅷ因子相关抗原,证明为内皮细胞。     

颅脑火器伤早期脑微血管三维构型和超微结构实验研究

目的 探讨颅脑火器伤早期猫脑微血管三维构型和超微结构变化。方法 应用脑微血管铸型扫描电镜观察伤后脑微血管三维结构的变化，同时行脑超薄切片透射电镜观察微血管内皮细胞和神经细胞结构的变化。结果 颅脑火器伤后，脑微血管发生紊乱，既有血管痉挛又有血管扩张，出血和缺血并存，微血管括约肌出现病理性强力收缩，部分微血管破裂，血管内皮细胞微绒毛、空泡增多，紧密连接增宽，基底膜断裂，血管周围水肿及周围神经细胞损伤。结论 颅脑火器伤早期脑微血管结构出现损害，引起了脑微循环功能紊乱，导致脑的继发性损害。     

大鼠心脏微血管内皮细胞原代培养方法研究

目的探讨大鼠心脏微血管内皮细胞的原代培养技术,以获取纯化的大鼠心脏微血管内皮细胞。方法应用体质量80 g的雄性Wistar大鼠左心室的微血管内皮细胞进行原代培养并传代培养;通过倒置显微镜扫描观察其形态,同时对培养细胞采用免疫组织化学荧光染色法进行内皮表面因子(vWF)鉴定,并用Hoechst染色,于荧光倒置显微镜下观察。结果体外培养的大鼠微血管内皮细胞呈梭形,形成单层后呈典型的鹅卵石样或铺路石样排列。采用Hoechst染色后,可见90%以上的细胞胞浆和核膜周围被染成红色,细胞核呈蓝色荧光;merge 3染色见细胞胞浆和核膜周围被染成黑色,细胞核呈蓝色;cy-3染色见细胞胞浆和核膜周围被激发出红色光。证明培养细胞为血管内皮细胞。结论通过改进的微血管内皮细胞分离方法培养获得的细胞,生长状态良好、纯度高,且能够稳定地传代培养。     

兔眼爆炸伤后早期角膜内皮细胞扫描电镜下改变

角膜爆炸伤是临床常见的眼外伤。临床观察发现，伤后出现早期角膜水肿、浑浊．中期新生血管长人浑浊角膜，晚期大量新生血管及瘢痕形成等病变。但有关角膜爆炸伤后的病理改变，尤其超微结构的病理改变报道较少。本研究通过对兔眼角膜爆炸伤的角膜内皮细胞进行扫描电镜动态观察，希望能为临床治疗和预后判断提供依据。     

颅脑火器伤早期脑微血管三维构型和超微结构实验研究

目的 探讨颅脑火器伤早期猫脑微血管三维构型和超微结构变化。方法 应用脑微血管铸型扫描电镜观察伤后脑微血管三维结构的变化,同时行脑超薄切片透射电镜观察微血管内皮细胞和神经细胞结构的变化。结果 颅脑火器伤后,脑微血管发生紊乱,既有血管痉挛又有血管扩张,出血和缺血并存,微血管括约肌出现病理性强力收缩,部分微血管破裂,血管内皮细胞微绒毛、空泡增多,紧密连接增宽,基底膜断裂,血管周围水肿及周围神经细胞损伤。结论 颅脑火器伤早期脑微血管结构出现损害,引起了脑微循环功能紊乱,导致脑的继发性损害。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.