百合多糖对小鼠免疫功能的影响

目的：观察百合多糖（LP）对小鼠免疫功能的影响．方法：环磷酰胺复制免疫抑制模型；测定胸腺、脾脏质量并计算脏器系数；鸡红细胞法测定单核巨噬细胞吞噬功能；绵羊红细胞法测定血清溶血素IgG，IgM含量；MTT法测定小鼠脾细胞的增殖．结果：在100～400mg／kg剂量范围内，LP可以提高免疫抑制模型小鼠的免疫器官指数，促进其吞噬指数和腹腔巨噬细胞增殖反应，提高其血清溶血素IgG，IgM含量；在75～150mg／L剂量范围内能促进小鼠脾细胞的增殖．结论：百合多糖能增强免疫抑制小鼠的非特异性和特异性免疫功能．     

玉丹荣心丸对小鼠免疫调节功能影响的实验研究

目的 探讨玉丹荣心丸对小鼠免疫功能的调节作用.方法 将小鼠随机分为正常组、Cy(环磷酰胺)模型组、黄芪组、荣心丸组,分别观察玉丹荣心丸对小鼠腹腔巨噬细胞的吞噬功能、2,4-二硝基氯苯(DNCB)所致迟发性超敏反应、鸡红细胞(CRBC)致敏小鼠溶血素抗体生成的影响.结果 荣心丸组小鼠与Cy模型组相比,巨噬细胞吞噬能力提高(P＜0.01),DNCB所致迟发性超敏反应增强(P＜0.01),血清溶血素抗体水平增加(P＜0.01).结论 玉丹荣心丸具有一定增强环磷酰胺所致免疫功能低下小鼠非特异性和特异性免疫功能的作用.     

人胎肝细胞对BALB/c小鼠免疫系统及其功能的影响

为了解人胎肝细胞 (FL C)对受体免疫系统及其功能的影响 ,作者采用 BAL B/ c小鼠进行了 FL C腹腔注射实验研究。实验分为空白对照和 FL C高、中、低剂量 (1 2 5× 1 0 5 ,2 5× 1 0 5 ,5× 1 0 5 胎肝细胞 /日 )组。结果显示 :FL C可增加小鼠胸腺脏器系数、外周血 T淋巴细胞数和淋巴细胞总数、脾细胞总数、抗体生成细胞数、淋巴细胞转化百分率和刺激指数 ,以及腹腔巨噬细胞吞噬作用 ,还可以影响 NK细胞活性。上述结果提示 ,人 FL C可增强小鼠非特异性免疫、体液免疫和细胞免疫功能 ,但对小鼠脾脏脏器系数无明显影响 ,也未见引起迟发型变态反应     

养神口服液对小鼠细胞免疫功能及非特异性免疫功能的影响

目的:了解养神口服液的保健功能。方法:对其进行小鼠的细胞免疫功能检测和非特异性免疫功能检测。结果:养神口服液可以显著增加小鼠的足跖肿胀度,明显提高小鼠的脾脏淋巴细胞增殖能力和小鼠腹腔巨噬细胞吞噬鸡红细胞的吞噬率和吞噬指数,而脾脏和胸腺的脏器系数无明显差异。结论:该养神口服液可以显著增强小鼠的细胞免疫功能和非特异性免疫功能。     

黄芪多糖对小鼠免疫功能的影响

目的观察黄芪多糖对小鼠免疫功能的影响。方法以黄芪多糖低、中、高浓度给小鼠连续灌胃7 d后,处死测定小鼠体重、脾脏及胸腺重量,计算脾指数与胸腺指数,观察小鼠免疫器官重量的变化;采用小鼠腹腔巨噬细胞吞噬鸡红细胞实验测试黄芪多糖对小鼠非特异性免疫的影响;采用溶血空斑形成实验测试黄芪多糖对小鼠体液免疫的影响。结果黄芪多糖能够增加小鼠免疫器官重量,提高巨噬细胞吞噬功能及增加B细胞产生抗体能力。结论黄芪多糖具有调节免疫功能,对非特异性免疫和体液免疫均有一定的增强作用。     

黄芪对免疫功能低下小鼠免疫功能的影响

[目的]研究黄芪注射液对环磷酰胺(cyclophosphamide,CTX)所致免疫力低下小鼠免疫功能的影响。[方法]以环磷酰胺建立免疫功能低下小鼠模型,分别腹腔注射不同剂量的黄芪注射液,观察各组小鼠对山羊红细胞的抗体应答反应、腹腔巨噬细胞的吞噬百分率和吞噬指数、以及胸腺指数和脾脏指数。[结果]环磷酰胺所致的免疫功能低下小鼠经腹腔注射黄芪注射液后,小鼠腹腔巨噬细胞吞噬率(P<0.05)和吞噬指数(P<0.01)明显增加,血清溶血素值显著上升(P<0.05),脾脏指数增加;而胸腺指数表现为下降趋势,但注射高剂量黄芪的小鼠胸腺指数仍比低剂量小鼠高。[结论]黄芪对免疫功能低下小鼠的体液免疫功能和巨噬细胞吞噬功能具有增强作用。     

寿尔康对小鼠免疫功能的影响

应用免疫学技术研究寿尔康(SEK)对小鼠免疫功能的影响。研究发现SEK经口服给药可明显增加小鼠脾脏及胸腺重量,提高小鼠腹腔巨噬细胞的吞噬率和吞噬指数,增加ANAE~+淋巴细胞百分率,促进小鼠外周血溶血素的生成和脾脏抗体细胞分泌溶血素的功能,明显增加小鼠体内NK细胞的活性,使cpm值增大;在体外可提高小鼠NK细胞的杀伤活性;并能增加对小鼠体内YAC-1细胞的清除。提示SEK可增强小鼠特异性和非特异性免疫功能,提高NK细胞的杀伤活性。     

孕晚期小鼠脾脏巨噬细胞抗原提呈功能的改变

目的　研究孕晚期 Balb/ c小鼠脾脏巨噬细胞抗原提呈功能的改变。方法　分离孕晚期小鼠脾脏巨噬细胞 ,以动情期小鼠作为对照。通过诱导迟发超敏反应和体外淋巴细胞抗原特异性转化实验检测巨噬细胞使机体初始 T致敏的能力以及诱导致敏 T细胞增殖的能力。结果　将充分摄取和结合了抗原 HGG或 Pla- Ag的孕晚期小鼠脾脏巨噬细胞注射到动情期小鼠腹腔使之致敏 ,再用相同抗原注射到小鼠足底诱发的迟发超敏反应强度显著低于动情期小鼠 (P<0 .0 5 )。当孕晚期小鼠脾脏巨噬细胞被当作抗原提呈细胞时 ,体外用人γ球蛋白抗原或胎盘抗原诱导致敏的正常小鼠的 T细胞增殖强度也显著低于动情期小鼠 (P<0 .0 5 )。孕晚期小鼠脾脏巨噬细胞还能显著抑制动情期小鼠脾脏巨噬细胞刺激致敏 T细胞增殖的能力。结论　孕晚期 Balb/ c小鼠脾脏巨噬细胞的抗原提呈功能显著受到抑制     

G-CSF和GM-CSF动员小鼠造血干/祖细胞过程中对外周血T淋巴细胞亚群的影响

目的观察粒细胞集落刺激因子（G-CSF）和粒-巨噬细胞集落刺激因子（GM-CSF）单独、同时或序贯联合应用动员小鼠外周造血干细胞时,外周血T细胞亚群的变化。方法72只BALB/c小鼠随机分成A、B、C、D、E、对照组6组,皮下注射：A组,重组人（rh）G-CSF 400μg·kg^-1·d^-1;B组,rhGM-CSF 400μg·kg^-1·d^-1;C组,rhG-CSF 200μg·kg^-1·d^-1＋rhGM-CSF 200μg·kg^-1·d^-1;D组,rhGM-CSF 400μg·kg^-1·d^-1（前3天）＋rhG-CSF 400μg·kg^-1·d^-1（后2天）;E组,rhG-CSF 400μg·kg^-1·d^-1（前3天）＋rhGM-CSF400μg·kg^-1·d^-1（后2天）;对照组,生理盐水0.2 ml,连续注射5天。动态观察外周血白细胞计数,同时通过流式细胞仪测定外周血T细胞亚群的变化。结果rhG-CSF与rhGM-CSF联合方案动员外周血白细胞计数较高;动员后各实验组CD3^＋细胞、CD3^＋CD8^＋细胞、CD3^＋CD4^-CD8^-细胞增加,CD3^＋CD4^＋/CD3^＋CD8^＋比值下降,GM-CSF明显提高了CD3^＋CD4^-CD8^-细胞比例。结论G-CSF和GM-CSF动员小鼠外周血造血干/祖细胞过程中对T淋巴细胞亚群的数量和比例有一定的影响,其可能有免疫调节作用。     

小儿复感宁合剂增强机体免疫功能的实验研究

目的:探讨小儿复感宁合剂对机体免疫功能的影响。方法:将50只小鼠随机分为5组,A,B组灌蒸馏水,C组予贞芪扶正胶囊,D,E组予小儿复感宁合剂。给药第8 d皮下注射环磷酰胺造模,分别观察小儿复感宁合剂对小鼠腹腔巨噬细胞吞噬功能、2,4-二硝基氯苯(DNCB)所致迟发性超敏反应、鸡红细胞(CRBC)致敏小鼠溶血素抗体生成的影响。结果:小儿复感宁合剂能增强小鼠腹腔巨噬细胞吞噬功能;增强DNCB所致迟发性超敏反应;提高小鼠血清溶血素抗体水平。结论:小儿复感宁合剂能增强环磷酰胺所致免疫功能低下小鼠非特异性免疫功能和特异性免疫功能。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.