Embelin逆转白血病耐药细胞株K562/D对柔红霉素的耐药性

目的 证明酸藤子(embelin)可以逆转白血病耐药细胞株K562/D对柔红霉素(DNR)的耐药性.方法 应用MTT法求出embelin作用于K562/D 24 h后的IC10以及K562/D对DNR的耐药指数和逆转倍数;应用annexin V FITC/PI复染流式细胞仪检测细胞凋亡;应用JC-1染色流式细胞仪检测线粒体膜电位(△Ψm).结果 K562/D细胞对DNR耐药,耐药指数为7.72;小剂量embelin(5μg/ml)可以逆转K562/D细胞对DNR耐药性,逆转倍数为7.46;embelin(5μg/ml)与DNR(0.1 μg/ml)联合作用于K562/D细胞6 h后△Ψm即出现明显去极化,细胞凋亡率呈时间依赖性升高,与对照组比较有统计学差异.结论 embelin 可以通过促进细胞凋亡逆转K562/D对DNR的耐药性,并且在凋亡早期△Ψm已经出现明显去极化,说明凋亡不可逆转.     

抗人脑胶质瘤单克隆抗体导向药物的选择性细胞毒作用

采用DextranT-40桥联法将抗人脑胶质瘤单克隆抗体SZ-39与抗肿瘤药物阿霉素进行化学交联,制备成免疫交联物。SZ-39单抗和阿霉素的摩尔结合比为1:48。~3H-TdR掺入细胞毒性试验表明,免疫交联物对靶细胞SHG-44的细胞毒作用显著优于游离药物、游离抗体及药物和抗体的混合物。其半数抑制剂量IC_(50)为1.75×10~(-8)mol/L。免疫交联物对非靶细胞SP_2/O的细胞毒作用不及游离药物。实验显示免疫交联物有良好的靶选择性细胞毒作用。     

前列腺癌单克隆抗体与平阳霉素偶联物导向化疗的体外作用

目的 探讨导向化疗对前列腺癌细胞系LNCaP的体外作用.方法 以葡聚糖为中介体制备抗前列腺癌单克隆抗体7E11C5.3与平阳霉素(PYM)的偶联物,用间接ELISA法测定其免疫活性,2,3,5-氯化三苯基四氮唑法测定其抑菌活性.四唑盐法测定其体外细胞毒作用.结果 偶联物中7E11C5.3和PYM的摩尔比为1:54,偶联后单抗的免疫活性降低了10%～20%,偶联物中PYM的抑菌活性为游离PYM的25%,偶联物和游离PYM对体外培养的LNCaP细胞50%抑制浓度分别为(9.41±1.98)μg/ml和(29.92μ7.88)μg/ml.结论 7E11C5.3-PYM偶联物的体外抗前列腺癌作用强于游离PYM.     

苦参碱联合抗肿瘤药抑制K562细胞增殖的研究

目的观察苦参碱(Ma)及Ma联合长春新碱(VCR)、阿糖胞苷(Ara-c)、三尖杉酯碱(HRT)、阿霉素(ADM)、柔红霉素(DNR)对K562细胞增殖的影响.方法用MTT比色法测定Ma及Ma联合常用抗肿瘤药物对K562细胞的抑制率.结果Ma(160～400μg/ml)对K562细胞有明显抑制作用.Ma(80 μg/ml)与VCR、Ara-c、HRT、ADM、DNR分别合用时抑制率较单用VCR、Ara-c、HRT时的抑制率高(P＜0.05),但较单用ADM、DNR时无明显增加抑制作用.结论Ma对K562细胞增殖具有抑制作用,呈剂量相关性.Ma能增强VCR、Ara-c、HRT对K562细胞增殖的抑制作用.     

氧化苏木素抑制ABCB1介导肿瘤多药耐药活性的研究

目的探讨氧化苏木素对ABCB1介导的肿瘤多药耐药活性的影响抑制。方法 MTT试验检测氧化苏木素对人白血病K562及其耐药K562/ADR(ABCB1高表达)细胞的细胞毒作用;Annexin V/PI双染,流式细胞仪检测细胞凋亡的发生。结果氧化苏木素对耐药的K562/ADR细胞具有高效的细胞毒作用,氧化苏木素对K562和K562/ADR细胞的IC50值分别为(5.45±0.36)和(5.62±0.43)μmol/L,二者无显著性差异。流式细胞仪检测凋亡的结果显示:0、5、10、20μmol/L的氧化苏木素分别处理K562和K562/ADR细胞后,K562细胞的凋亡率从(3.41±0.55)%依次升高到(24.67±2.08)%、(40.33±3.51)%和(66.67±3.64)%,K562/ADR细胞的凋亡率从(2.82±0.57)%依次升高到(23.01±3.60)%、(38.23±4.06)%和(65.77±5.51)%。结论氧化苏木素克服了ABCB1介导的肿瘤多药耐药活性,诱导耐药细胞凋亡是其作用的机制。     

重组人粒细胞集落刺激因子对K562细胞凋亡和其细胞毒药物敏感性的影响

目的探讨重组人粒细胞集落刺激因子(rhu G-CSF)对K562细胞凋亡和其细胞毒药物敏感性的影响.方法采用流式细胞术和MTT法分别测定细胞凋亡和细胞毒药物敏感性.结果在低血清培养条件下,rhu G-CSF对K562细胞的凋亡有抑制作用,其凋亡百分率与rhu G-CSF浓度呈负线性相关;rhu G-CSF可增加K562细胞对Ara-C的敏感性,而降低其对DNR的敏感性;在Ara-C和DNR联合条件下,rhu G-CSF能增强两药的联合细胞毒毒性,克服了单用DNR条件下,其使DNR对K562细胞毒毒性作用降低的情况.结论虽然rhu G-CSF并不降低DNR和Ara-C对K562细胞的联合细胞毒毒性,但当其和化疗药物同时使用治疗髓系白血病患者时,仍应慎重.     

环孢素A联合苯基棕榈酰胺吗啡丙醇对K562/AO2细胞GCS基因表达的影响及对逆转多药耐药

目的 研究环孢素A(CsA)和苯基棕榈酰胺吗啡丙醇(PPMP)联合应用对人白血病多药耐药细胞株K562/AO2的葡萄糖神经酰胺合酶(GCS)基因表达的影响及对白血病多药耐药的逆转作用,探索逆转白血病细胞耐药的策略.方法 应用Alamar BlueTM多功能细胞染色法测定阿霉素(ADR)对K562和K562/AO2细胞的半数抑制浓度(IC50)及判断CSA的非细胞毒性剂量;采用RT-PCR法检测GCS基因和mdr1基因的mRNA表达.结果 CsA浓度低于4.5μg/ml对K562/AO2细胞无细胞毒性.ADR对K562和K562/AO2细胞的IC50分别为(0.84±0.04)μg/ml和(89.24±1.27)μg/ml;当1μg/ml CsA和25μmol/L PPMP单用或联合作用于K562/AO2细胞时,ADR的IC50分别为(7.81±0.74)、(17.49±0.53)、(2.82±0.07)μg/ml;而低浓度CsA(0.01μg/ml)和PPMP(10 μmol/1)联合作用时IC50为(16.08±1.25)μg/ml.CsA联合PPMP可明显降低GCS基因的mRNA表达,低浓度CsA和PPMP联用也有此作用.结论 CsA联合PPMP可通过抑制K562/AO2细胞的GCS基因mRNA表达有效逆转其耐药,低浓度CsA联合PPMP在逆转其耐药的同时降低了细胞毒性.     

地塞米松对柔红霉素诱导K562细胞核因子kappa B活化及凋亡的影响

目的探讨地塞米松（DXM）对柔红霉素（DNR）诱导K562细胞核因子kappaB（NF—κB）活化及凋亡的影响。方法采用免疫组化二步法检测K562细胞的NF—κB活化,用流式细胞检测术检测K562细胞的凋亡率。结果DNR同时具有诱导K562细胞凋亡和NF—κB活化的作用;DXM50μg／L能显著抑制DNR诱导的K562细胞的NF—κB活化和增强DNR诱导的K562细胞凋亡,NF—κB活化抑制率为20．17％（P〈0．01）,细胞凋亡增强率为25．44％（P〈0．01）。结论DNR诱导K562细胞凋亡的同时激活NF—κB p65活化;DXM通过抑制NF—κB活化,增强其诱导K562细胞凋亡的作用。     

汉防己碱对白血病K562细胞生长的促进耐药作用

目的考察汉防己碱(Tet)对白血病K562细胞生长的影响。方法采用体外细胞培养,即在37℃、5%CO2、饱和湿度的培养箱中培养,培养液为含10%热灭活NBS的完全RPMI1640细胞培养基。待K562细胞与药物作用48 h后,收集并处理细胞,结合MTT和Western blot法研究Tet对K562细胞生长的作用特点。结果 Tet在0.33～1.00μg/ml仅使K562及K562/ADM细胞的存活率降低至75%～80%,低浓度Tet(0.33μg/ml)使阿霉素(ADM)和顺铂(cDDP)对K562细胞生长的IC50增加(P<0.01),1.0μg/ml Tet则降低IC50,使ADM对K562/ADM细胞的IC50增加,cDDP的IC50无明显变化。0.33μg/ml Tet有提升2种细胞的P-gp和MRP1表达的作用(P<0.05)。Tet各浓度对LRP表达无明显影响。结论 Tet对K562及K562/ADM细胞生长抑制作用具浓度依耐性,低浓度具加速耐药的作用,其促进耐药细胞发展的机制至少与提升P-gp和MRP1表达有关。     

环孢菌素A、汉防己甲素及两者联合逆转K562／A02细胞的多药耐药

目的：探讨环孢菌素A（CsA）、汉防己甲素（Tet）及两者联合应用对人白血病耐药细胞系K562／A02多药耐药的逆转作用。方法：采用M1Tr法测定柔红霉素（DNR）对细胞的半数抑制量（IC50），流式细胞仪检测细胞凋亡率和细胞内DNR浓度。结果：DNR对K562细胞和K562／A02细胞IC50分别为0．27和21．22mg·L^-1；CsA（1mg·L^-1）及Tet（0．1mg·L^-1）单独和联合处理K562／A02后，DNR的IC50分别为7．83、5．32和2．32mg·L^-1；CsA和Tet对DNR作用K562的IC50无明显影响；K562／A02细胞48h凋亡率为2．19％，DNR（1mg·L^-1）作用K562／A02细胞凋亡率为2．70％，CsA和Tet单独及联合处理后凋亡率分别为10．27％、17．64％和52．79％；两药处理后细胞内化疗药物DNR浓度亦明显增加。结论：CsA及Tet均可逆转耐药，且联合作用逆转效果更强。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.