呼和浩特地区2008-2009年婴幼儿轮状病毒性腹泻流行株分型研究

目的研究呼和浩特地区2008年6月～2009年5月婴幼儿腹泻轮状病毒(rotavirus,RV)的基因型。方法采集内蒙古妇幼保健院2008年6月～2009年5月所有≤59月龄的住院腹泻患儿粪便标本313份。采用酶联免疫吸附试验(ELISA)检测RV;对RV抗原阳性的标本用巢式逆转录聚合酶链式反应(nested RT-PCR)对6种流行的VP7基因型(G1、G2、G3、G4、G8、G9)和5种流行的VP4基因型(P[8]、P[4]、P[6]、P[9]、P[10])进行分型;最后整理数据,应用SPSS 13.0统计软件包进行统计分析。结果 313份粪便标本中,125份为RV抗原阳性(39.94%)。对阳性标本进行VP7基因分型,结果为G1型64.8%(81/125)、G2型2.4%(3/125)、G3型14.4%(18/125)、G9型0.8%(1/125)、G1+G3混合型16.8%(21/125)、G2+G3混合型0.8%(1/125);进行VP4基因分型,结果为P[8]型84.0%(105/125)、P[4]型4.0%(5/125)、P[8]+P[4]型1.6%(2/125),13份未分出型别。结论 RV是...     

柳城县婴幼儿腹泻轮状病毒流行特征分析

目的研究柳城县婴幼儿腹泻轮状病毒(RV)G型和P型的分型.方法2010年1~4月收集柳城县5个医疗机构就诊的婴幼儿腹泻粪便标本237份,采用A群轮状病毒诊断试剂盒(胶体金法)进行初筛,对RV初筛阳性标本用聚丙烯酰胺凝胶电泳、酶联免疫吸附及逆转录-聚合酶链反应进行RV检测和G、P分型.结果初筛检出89份阳性标本,阳性率37.55%;复检检出71份阳性标本,在71份阳性标本中G型分型为G1型8株(11.27%),G2型5株(7.04%),G3型38株(53.52%),G4型2株(2.82%),G9型10株(14.08%),混和型3株,未定型5株; P型分型为 P[8]型60株(84.51%),P[4]型5株(7.04%),P[6]4株(5.63%),P[9],P[10]型未检出,混合型1株,未定型1株,G型与P型组合以G3P[8]最常见,G9P[8]、G1P[8]、G2P[4]次之.结论柳城县婴幼儿RV流行型别以G3P[8]为主.     

呼和浩特地区2008-2009年婴幼儿轮状病毒性腹泻流行株分型研究

目的研究呼和浩特地区2008年6月～2009年5月婴幼儿腹泻轮状病毒（rotavirus,RV）的基因型。方法采集内蒙古妇幼保健院2008年6月～2009年5月所有≤59月龄的住院腹泻患儿粪便标本313份。采用酶联免疫吸附试验（ELISA）检测RV;对RV抗原阳性的标本用巢式逆转录聚合酶链式反应（nested RT-PCR）对6种流行的VP7基因型（G1、G2、G3、G4、G8、G9）和5种流行的VP4基因型（P[8]、P[4]、P[6]、P[9]、P[10]）进行分型;最后整理数据,应用SPSS 13.0统计软件包进行统计分析。结果 313份粪便标本中,125份为RV抗原阳性（39.94%）。对阳性标本进行VP7基因分型,结果为G1型64.8%（81/125）、G2型2.4%（3/125）、G3型14.4%（18/125）、G9型0.8%（1/125）、G1+G3混合型16.8%（21/125）、G2+G3混合型0.8%（1/125）;进行VP4基因分型,结果为P[8]型84.0%（105/125）、P[4]型4.0%（5/125）、P[8]+P[4]型1.6%（2/125）,13份...     

辽宁省首次检出G9型A组轮状病毒

目的了解辽宁省A组轮状病毒的感染情况,为轮状病毒的预防控制提供科学依据。方法采集辽宁省沈阳、大连、丹东、阜新4个市门诊及住院疑似病毒性腹泻患者粪便标本135份,采用酶联免疫吸附试验(ELISA)检测轮状病毒抗原,阳性标本用逆转录-聚合酶链反应(RT-PCR)扩增A组轮状病毒VP7基因和VP4基因,RT-PCR产物进行核苷酸碱基序列的测定和比对,并构建VP7基因遗传进化树。结果 135份粪便标本中,共检测出A组轮状病毒阳性标本21份;A组轮状病毒G基因分型:G9型15株,G3型2株,G2型1株,G1型1株,未分型2株;P基因型分型:P[8]型20株,P[4]型1株;G/P基因型组合以G9P[8]为主共15株,G3P[8]2株,G1P[8]1株,G2P[4]1株,G/P[8]2株。结论辽宁省首次检出G9型A组轮状病毒,主要流行G/P基因型组合为G9P[8]。     

北京地区2007-2008年G9型A组人轮状病毒VP7和VP4基因分析

目的 了解北京地区2007-2008年检测到的G9型A组人轮状病毒外壳蛋白VP7和VP4的基因特征.方法 选取经过轮状病毒核酸杂交方法检测为G9型轮状病毒的12份儿童腹泻患儿的粪便标本,应用针对VP7全长基因的特异引物对进行RT-PCR扩增,对所获得的VP7全长基因进行克隆和测序,将所获得的序列与GenBank中的G9型原型病毒株和近期流行株的VP7基因进行序列和种系进化分析;经巢式PCR对G9型的VP4进行P基因分型.结果 12株G9型轮状病毒经VP7基因的序列比较分析得到确认.P基因分型结果显示北京地区近年来存在G9P[8]和G9P[6]型两种组合的轮状病毒感染.序列和种系进化分析发现北京G9型株VP7基因与世界范围内近期流行的G9型株一样都属于进化分支Ⅲ,彼此间的核苷酸和氨基酸同源性较高,而与国内最早报道的G9型T203进化关系较远,且北京G9P[8]和G9P[6]型株分别与国内近期报道的新疆G9P[8]和G9P[6]型株及相应的武汉G9型株VP7基因,在氨基酸位点上存在一些共同的氨基酸残基取代.结论 北京地区近年存在G9P[8]和G9P[6]两种不同基因组合的G9型轮状病毒感染,需要进一步加强对G9型轮状病毒的分子流行病学监测.     

2009年广州地区轮状病毒分子流行病学研究

目的了解广州地区引起婴幼儿腹泻轮状病毒（Rotavirus,RV）的血清型特征。方法采用酶联免疫吸附试验（ELASA）及巢式逆转录聚合酶链式反应（NestRT—PCR）,对2009—01／12来自广州地区5间监测医院的5岁以下腹泻门诊患儿粪便标本,进行RV抗原检测和血清型分型。结果检测腹泻门诊患儿粪便361份,男性228份,女性133份,经ELISA法检测RV抗原阳性的标本146份（阳性率为40．44％）,男性阳性率39．04％,女性阳性率42．86％;7～12月龄RV腹泻患儿最多,占45．21％;秋冬季为（1月,10～12月）为发病高峰季节,占76．71％;对132份ELISA阳性标本进行G、P分型,G分型中主要为G1型（43．94％）,其他依次为G3型（28．03％）,G1＋G3混合型（24．24％）,G9型（1．52％）,G1＋G9型、G1＋G3＋G9型及未分型均为（0．76％）;P分型中主要为P[8]型（47．73％）,其他依次为P[4]型（25．76％）,P[4]＋P[8]混合型（23．48％）,未分型（1．52％）,P[6]＋P[8]混合型（0．76％）;G型和P型组合情况以G1P[8]型多见,33株,G3P[8]次之,18株。结论RV是广州地区2009年婴幼儿腹泻的主要病原,RV腹泻阳性率男女无差异,主要高发于秋冬季,RV发病以7～12月龄人数最多,G、P分型分别以G1和P[8]为主要流行毒株,组合型G3P[8]为流行优势株,混合感染亦很常见。     

婴幼儿轮状病毒性腹泻的临床特点及分子流行病学分析

目的:通过分子流行病学调查银川地区婴幼儿轮状病毒性腹泻的病原学特点和临床相关性分析。方法:采用酶联免疫吸附试验(ELISA)及逆转录-聚合酶链反应(RT-PCR)法对银川地区2009年7月～2010年6月收集的256例婴幼儿腹泻粪便标本进行轮状病毒(RV)检测。结果:在256例标本中应用ELISA法检测RV阳性率为60.2%(154/256),RT-PCR法阳性率为53.9%(138/256);其中G2型55份(39.9%)、G3型42份(30.4%)、未能分型26份(18.8%),不同G型混合感染8份(5.8%),G1型7份(5.1%),未发现G4和G9型。对标本进行P分型,P[4]基因型62份(44.9%),未能分型40份(29%),P[8]基因型36份(26.1%),无混合感染,未发现P[6]、P[9]、P[10]型。RV G型与P型的关系主要为P[4]G2、P[8]G3。结论:HRV是银川地区婴幼儿病毒性腹泻的最主要病原,临床症状重于非HRV腹泻。     

昆明市儿童医院1998～2001年轮状病毒哨点监测分析

目的 了解昆明市轮状病毒腹泻的流行状况。方法 以昆明市儿童医院为哨点监测,监测对象为5岁以下腹泻住院患儿,收集患儿的临床资料和粪便标本进行轮状病毒的检测和分型。病毒检测用聚丙烯酰胺凝胶电泳(PAGE)和酶联免疫吸附试验(ELISA),毒株分型用ELISA和/或反转录-聚合酶链反应(RT-PCR)。结果 3年监测中共收集466份腹泻患儿的粪便标本,轮状病毒的检出率为52.8％(246/466)。轮状病毒感染97％发生于2岁以下儿童。感染有明显的季节性,10～12月份是流行季节。对204份轮状病毒阳性标本进行G分型,G1型为流行优势株,占47.5％,其次为G2型(17.6％)、G3型(15.7％)G9型(4.9％)和G4型(1.0％)。P基因型以P[4]、P[8]和P[6]型为常见。最常见的P-G组合型是P[4]G2,占34.1％(14/41),其次是P[8]G1和P[6]G9,分别占29.3％(12/41)和12.2％(5/41),还有其他7种不常见的P-G组合的毒株类型。结论 轮状病毒是昆明地区儿童腹泻住院的主要病原,毒株呈现型的多样性,应该开发和应用轮状病毒疫苗预防控制其流行。     

2010年婴幼儿腹泻轮状病毒监测分析

目的了解乌鲁木齐地区婴幼儿轮状病毒(RV)腹泻流行情况。方法 2010年1~12月,采集门诊或住院≤5岁婴幼儿腹泻样本503份,用胶体金法和ELISA检测RV抗原,阳性样本用逆转录-聚合酶链反应(RT-PCR)进行G、P基因型分型。结果 503份腹泻样本,RV阳性率为24.25%(122/503)。RV感染对象主要为35月以内婴幼儿(95.08%)。对102份RV阳性样本进行G/P分型,G3型是主要优势株,占46.08%,其次为G1型(34.31%)、G9型(11.76%)和G2型(2.94%),混合G型感染占4.90%;P基因型以P[8]为主导,占67.65%,其次为P型混合感染(19.61%)、P[4](6.86%)和P[6](4.90%),1例(0.98%)未能分型。最常见的G/P组合为G3P[8](35.29%)和G1P[8](27.45%)。结论 RV是乌鲁木齐地区婴幼儿腹泻的主要病原,2010年G3P[8]和G1P[8]为主要流行基因型。     

甘肃武威市门诊腹泻儿童轮状病毒和诺如病毒腹泻流行特征分析

目的 分析武威市5岁以下儿童轮状病毒和诺如病毒腹泻的流行病学特征。方法 收集武威市两所哨点医院2012-2014年门诊腹泻患儿粪便标本,采用酶联免疫吸附实验(ELISA)法检测轮状病毒(RV)抗原,检出的阳性标本用逆转录-聚合酶链反应(RT-PCR)进行G/P基因分型;采用RT-PCR检测诺如病毒(NoV),分析流行病学特征。结果 共采集182份粪便标本,A组RV抗原阳性率为29.12%,男女性别差异有统计学意义(χ²=182.000,P<0.001);2岁以下儿童为RV 高发人群,高发与低发年龄段RV阳性率差异有统计学意义(χ²=219.576,P<0.001);发病高峰出现在5月、11月,5月份阳性率最高,高发与低发月份阳性率差异有统计学意义(χ²=279.000,P<0.001 )。RV G、P分型,以G9、G3、P[8]型为主要流行株,G、P型组合模式分析显示:最常见的是G9+P[8],其次是G3+P[8]。NoV抗原阳性率为15.93%,病例年龄均为2岁以下儿童;发病也呈现两个高峰,分别为4月最高峰和12月次高峰,高发与低发月份阳性率差异具有统计学意义(χ²=159.641,P<0.001);对NoV阳性标本进行分型,GI型占55.17%,GII型占44.83%。A组RV和NoV混合感染阳性率为6.04%。结论 RV和NoV是武威市5岁以下儿童腹泻的主要病原体,应根据流行特征做好预防工作。     

兰州地区2004-2005年度婴幼儿病毒性腹泻的病原学研究

目的了解兰州地区主要四种腹泻病毒的流行病学特点.方法收集兰州大学第一医院儿科2004年7月至2005年6月5岁以下全部住院腹泻患儿400例的粪便标本,分别采用Dako公司酶免疫试剂盒检测轮状病毒、星状病毒、腺病毒,杯状病毒检测采用酶免疫法和逆转录-聚合酶链反应（RT-PCR）法.对轮状病毒、星状病毒阳性标本用RT-PCR进行毒株分型鉴定.结果400份标本中四种病毒检测阳性率依次为轮状病毒47.3%、杯状病毒15.5%、星状病毒9.5%、腺病毒7.5%.其中有混合感染的病例数占13.5%.轮状病毒毒株G血清型分型结果为G2（34.4%）、G3（32.8%）、G1（1.1%）、不同型混合感染（5.8%）、未能分型（25.9%）,P基因型分型结果为P[4]（45%）、P[8]（22.1%）、未能分型（32.9%）.G型与P型组合P[4]G2（43.6%）、P[8]G3（25.6%）,P[4]G3（13.8%）、P[8]G2（3.2%）、P[4]G1和P[8]G1各1例.星状病毒血清分型结果为1型（57.8%）、3型（2.6%）、8型（2.6%）、未能分型（36.8%）.病毒性腹泻的高发季节轮状病毒最为明显为10-12月份.发病年龄主要为2岁以下婴幼儿,轮状病毒的高发年龄是6-23月龄.结论兰州地区婴幼儿病毒性腹泻的病原复杂,轮状病毒仍是最主要病原,该年度轮状病毒的主要流行株为P[4]G2,与往年明显不同,病原混合感染比例较大,值得重视.     

Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: Genetic relationships with other G9 strains and detection of a new G9 subtype

Group A rotaviruses (RV-A) are the leading cause of viral gastroenteritis in children worldwide and genotype G9P[8] is one of the five most common genotypes detected in humans. In order to gain insight into the degree of genetic variability of G9P[8] strains circulating in Cameroon, stool samples were collected during the 1999–2000 rotavirus season in two different geographic regions in Cameroon (Southwest and Western Regions). By RT-PCR, 15 G9P[8] strains (15/89 = 16.8%) were identified whose genomic configurations was subsequently determined by complete or partial gene sequencing. In general, all Cameroonian G9 strains clustered into current globally-spread sublineages of the VP7 gene and displayed 86.6–100% nucleotide identity amongst themselves and 81.2–99.5% nucleotide identity with global G9 strains. The full genome classification of all Cameroonian strains was G9-P[8]-I1–R1–C1–M1–A1–N1–T1–E1–H1 but phylogenetic analysis of each gene revealed that the strains were spread across 4 or more distinct lineages. An unusual strain, RVA/Human-wt/CMR/6788/1999/G9P[8], which shared the genomic constellation of other Cameroonian G9P[8] strains, contained a novel G9 subtype which diverged significantly (18.8% nucleotide and 19% amino acid distance) from previously described G9 strains. Nucleotide and amino acid alignments revealed that the 3′ end of this gene is highly divergent from other G9 VP7 genes suggesting that it arose through extensive accumulation of point mutations. The results of this study demonstrate that diverse G9 strains circulated in Cameroon during 1999–2000.     

长春市儿童医院1998～2001年轮状病毒哨点监测分析

目的 为在中国开发和应用轮状病毒疫苗提供流行病学背景资料。方法 以医院为基础的哨点监测,在长春市儿童医院5岁以下腹泻患儿中进行,收集患儿临床资料和粪便标本,轮状病毒检测用聚丙烯酰胺凝胶电泳(PAGE)和／或酶联免疫吸附试验(ELISA),毒株分型用ELISA和／或逆转录-聚合酶链反应(RT—PCR)。所有资料录入计算机进行数据分析。结果 3年监测中共调查2343例腹泻患儿,收集便样1211份,轮状病毒检出率门诊患儿和住院患儿分别为31．0％和52．9％。轮状病毒感染95．0％发生于2岁以下儿童。每年寒冷季节流行(11月到次年3月)。流行的轮状病毒G血清型依次为G1(82．4％)、G2(5．0％)、G3(3．3％)和G4(0．9％),P基因型以P[8]和P[4]为常见。共检出9种P～G结合的毒株,其中世界常见的4种流行株占75．6％。结论 轮状病毒流行毒株呈现超常多样性,轮状病毒是长春地区儿童重症腹泻的主要原因。     

Sequence analysis of the whole genomes of five African human G9 rotavirus strains

The G9 rotaviruses are amongst the most common global rotavirus strains causing severe childhood diarrhoea. However, the whole genomes of only a few G9 rotaviruses have been fully sequenced and characterised of which only one G9P[6] and one G9P[8] are from Africa. We determined the consensus sequence of the whole genomes of five African human group A G9 rotavirus strains, four G9P[8] strains and one G9P[6] strain collected in Cameroon (central Africa), Kenya (eastern Africa), South Africa and Zimbabwe (southern Africa) in 1999, 2009 and 2010. Strain RVA/Human-wt/ZWE/MRC-DPRU1723/2009/G9P[8] from Zimbabwe, RVA/Human-wt/ZAF/MRC-DPRU4677/2010/G9P[8] from South Africa, RVA/Human-wt/CMR/1424/2009/G9P[8] from Cameroon and RVA/Human-wt/KEN/MRC-DPRU2427/2010/G9P[8] from Kenya were on a Wa-like genetic backbone and were genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Strain RVA/Human-wt/ZAF/MRC-DPRU9317/1999/G9P[6] from South Africa was genotyped as G9-P[6]-I2-R2-C2-M2-A2-N1-T2-E2-H2. Rotavirus A strain MRC-DPRU9317 is the second G9 strain to be reported on a DS-1-like genetic backbone, the other being RVA/Human-wt/ZAF/GR10924/1999/G9P[6]. MRC-DPRU9317 was found to be a reassortant between DS-1-like (I2, R2, C2, M2, A2, T2, E2 and H2) and Wa-like (N1) genome segments. All the genome segments of the five strains grouped strictly according to their genotype Wa- or DS-1-like clusters. Within their respective genotypes, the genome segments of the three G9 study strains from southern Africa clustered most closely with rotaviruses from the same geographical origin and with those with the same G and P types. The highest nucleotide identity of genome segments of the study strains from eastern and central Africa regions on a Wa-like backbone was not limited to rotaviruses with G9P[8] genotypes only, they were also closely related to G12P[6], G8P[8], G1P[8] and G11P[25] rotaviruses, indicating a close inter-genotype relationship between the G9 and other rotavirus genotypes. Rotavirus strain MRC-DPRU9317 is the first G9P[6] to be characterised on a DS-1-like genetic backbone with a reassortant segment 8 (NSP2) and fourth G9P[6] to be fully sequenced globally.     

Large-scale whole genome sequencing identifies country-wide spread of an emerging G9P[8] rotavirus strain in Hungary, 2012

With the availability of rotavirus vaccines routine strain surveillance has been launched or continued in many countries worldwide. In this study relevant information is provided from Hungary in order to extend knowledge about circulating rotavirus strains. Direct sequencing of the RT-PCR products obtained by VP7 and VP4 genes specific primer sets was utilized as routine laboratory method. In addition we explored the advantage of random primed RT-PCR and semiconductor sequencing of the whole genome of selected strains. During the study year, 2012, we identified an increase in the prevalence of G9P[8] strains across the country. This genotype combination predominated in seven out of nine study sites (detection rates, 45–83%). In addition to G9P[8]s, epidemiologically major strains included genotypes G1P[8] (34.2%), G2P[4] (13.5%), and G4P[8] (7.4%), whereas unusual and rare strains were G3P[8] (1%), G2P[8] (0.5%), G1P[4] (0.2%), G3P[4] (0.2%), and G3P[9] (0.2%). Whole genome analysis of 125 Hungarian human rotaviruses identified nine major genotype constellations and uncovered both intra- and intergenogroup reassortment events in circulating strains. Intergenogroup reassortment resulted in several unusual genotype constellations, including mono-reassortant G1P[8] and G9P[8] strains whose genotype 1 (Wa-like) backbone gene constellations contained DS1-like NSP2 and VP3 genes, respectively, as well as, a putative bovine–feline G3P[9] reassortant strain. The conserved genomic constellations of epidemiologically major genotypes suggested the clonal spread of the re-emerging G9P[8] genotype and several co-circulating strains (e.g., G1P[8] and G2P[4]) in many study sites during 2012. Of interest, medically important G2P[4] strains carried bovine-like VP1 and VP6 genes in their genotype constellation. No evidence for vaccine associated selection, or, interaction between wild-type and vaccine strains was obtained. In conclusion, this study reports the reemergence of G9P[8] strains across the country and indicates the robustness of whole genome sequencing in routine rotavirus strain surveillance.     

中国2006年麻疹野病毒血溶素基因特征分析

目的研究中国2006年流行的麻疹野病毒代表株血溶素蛋白（Fusion Protein,F）基因的分子特征,与其他年份毒株相比分析F基因的变异规律。方法从2006年各省（自治区、直辖市）送检的麻疹野病毒中选取9株代表株,使用逆转录-聚合酶链反应扩增病毒的F基因全长,并进行核苷酸序列测定,同时与中国疫苗株及其他年份毒株进行序列比对及基因亲缘性关系分析。结果2006年9株中国流行麻疹野病毒代表株,其核苷酸同源性为98．5％-99．8％,氨基酸同源性为99％-100％;与中国疫苗株沪。相比,其核苷酸同源性为95．4％-96．2％,氨基酸同源性为96．7％～97．2％;与1999～2003年代表株相比,其核苷酸同源性为97．9％-99．7％,氨基酸同源性为98．1％-100％。F基因3个糖基化位点（第32、64、70位氨基酸）、对病毒整合功能起着重要作用的第112位精氨酸（Arg）、第195位亮氨酸（Leu）未发生改变。结论中国2006年流行的麻疹病毒F基因变异不大,与其他年份毒株相比变异也不明显。F蛋白的重要功能位点未发生变异,F蛋白的变异与2006年麻疹流行无相关性。但由于F蛋白对病毒感染细胞和促使机体产生抗体具有重要意义,对F蛋白变异规律进行常规监测,对了解麻疹病毒变异规律和评价疫苗的保护效果具有重要意义。     

A DS-1 like G9P[6] human strain CDC-6 as a new rotavirus vaccine candidate

Human rotavirus vaccine Rotarix® (G1P[8]) has shown broad cross protection against homotypic and heterotypic Wa-like human rotavirus strains among children worldwide. This vaccine, however, appears to induce slightly less or non-consistent protection against DS-1 like rotavirus P[4] strains in some settings. In addition, children who are secretor or Lewis-negative and are vaccinated with Rotarix® often experience breakthrough infection with P[6] strains. By contrast, P[6] strains infect all children, irrespective of their secretor or Lewis status. In the present study, we report successful adaptation of a DS-1 like human rotavirus G9P[6] strain (CDC-6) to high growth in Vero cells and identify sequence changes that may be critical for enhanced growth in vitro and attenuation in vivo. This human G9P[6] strain could serve as a promising new and potential low-cost vaccine candidate for global use, particularly in targeted population with secretor or Lewis-negative status and high prevalent DS-1 like P[6] strains.     

High incidence of G9P181 rotavirus infections in Italian children during the winter season 1999-2000

We report a significant high incidence of infection with G9P[8] rotavirus in Italian children during the winter epidemic season 1999–2000. The study was carried out on 439 children <4 years hospitalized with acute diarrhea in Palermo. G9P[8] strains constituted 19% of all rotavirus identified and were not associated with more severe forms of gastroenteritis.     

Prevalence and genome characterization of porcine rotavirus A in southern Mozambique

Rotavirus A (RVA) is an important pathogen causing gastroenteritis in many species, including humans and pigs. The objective of this study was to determine the prevalence of RVA in pigs from smallholdings and commercial farms in southern Mozambique and characterize the complete genomes of selected strains. RVA was detected at a rate of 11.8% (n = 288), of which 7.6% was detected at commercial farms and 4.2% at smallholdings. The whole genomes of eight rotavirus strains were determined using an Illumina MiSeq platform. Seven displayed a G9P[13] and one a G4P[6] genotype combination, all with a typical porcine backbone (I1/5-R1-C1-M1-A1/8-N1-T1/7-E1-H1). Phylogenetic analysis indicated that the seven G9P[13] strains were in fact one strain that circulated on a commercial pig farm. The genome segments of this strain clustered with diverse segments of human and porcine RVA strains from various Asian countries. Analysis of the G4P[6] strain revealed four distinct genome segments (VP2, VP4, VP6 and VP7) and five genome segments closely related to South African porcine rotavirus strains (NSP1, NSP3, NSP4, NSP5 and VP1). These results suggest that both the G4P[6] and the G9P[13] strains possibly emerged through multiple reassortment events. The presence of these strains on the commercial farms and smallholdings calls for a more in-depth surveillance of rotavirus in Mozambique.