TNF-α在鼻息肉组织中的表达和意义

目的 :探讨肿瘤坏死因子 α(tumornecrosisfactor α ,TNF α)在鼻息肉中的表达和意义。方法 :采用放射免疫分析法研究TNF α在鼻息肉组织匀浆、胎儿下鼻甲组织匀浆和鼻息肉患者的血清、正常成人血清及脐血中的表达及其意义。结果 :鼻息肉组织匀浆、鼻息肉患者的血清、正常成人血清中TNF α平均质量浓度 (μg/L)分别为 1 0 3± 0 14 ,0 79± 0 11,0 79± 0 13,鼻息肉组织匀浆中TNF α的含量高于鼻息肉患者的血清、正常成人血清中的含量 ,差异显著 (P <0 0 1)。结论 :TNF α在鼻息肉中过度表达 ,通过局部炎症微环境的调控 ,在鼻息肉的发生、发展过程中起重要作用     

肝硬化患者血浆抗坏血酸和维生素E的测定

目的 :探讨肝硬化患者血浆抗坏血酸和维生素 E的水平及其意义。方法 :测定 6 3例肝硬化患者和 6 3例年龄及性别配对的正常人血浆抗坏血酸 ,维生素 E和过氧化脂质的含量。结果 :肝硬化组血浆抗坏血酸含量为( 42 .94± 6 .99)μm ol/ L ,明显低于对照组 ( 53.30± 9.4 5)μm ol/ L ( t=9.50 ,P =0 .0 0 0 ) ;肝硬化组血浆维生素 E含量为 ( 17.99± 3.51) μm ol/ L,明显低于对照组 ( 2 4 .59± 7.2 2 ) μm ol/ L( t=7.94 ,P=0 .0 0 0 ) ;肝硬化组血浆过氧化脂质含量为 ( 14 .0 9± 1.2 8) μm ol/ L,明显高于对照组 ( 12 .11± 1.2 0 ) μmol/ L( t=17.2 1,P=0 .0 0 0 )。而且肝硬化患者的三个指标之间均有良好的相关性 ( P=0 .0 0 0 )。结论 :肝硬化患者血浆抗坏血酸和维生素 E水平显著下降 ,体内氧化和抗氧化系统失衡。     

血浆亚硝酸根及环磷酸鸟苷浓度预测早产的价值

目的　探讨血浆亚硝酸根 (NO2 - )及环磷酸鸟苷 (c GMP)浓度预测早产的价值。方法　采用示波极谱法和放射免疫法测定了 2 5 0例妊娠妇女 (2 10例正常妊娠妇女和 4 0例早产妇女 )血浆 NO2 - 和 c GMP浓度。结果　正常妊娠妇女从 2 8周至 36 + 6周 ,血浆 NO2 -和 c GMP浓度均逐渐增加。NO- 2 从 1.95 75± 0 .35 0 9增加至 2 .2 36 0±0 .3486μmol/ L ,c GMP从 31.16 2 5± 3.90 82增加至 4 2 .185 0± 11.3816μm ol/ L。血浆 NO2 - 浓度在 34～ 34+ 6 周达最高值 (2 .2 4 2 5± 0 .340 7μm ol/ L) ,血浆 c GMP浓度在 33～ 33+ 6周达最高值 (49.0 0 10± 8.6 719μm ol/ L)。早产妇女血浆 NO2 -和 c GMP浓度均低于相应孕周的正常妊娠妇女 (P<0 .0 1和 P<0 .0 5 )。结论　血浆 NO2 -和 c GMP浓度预测早产具有良好的可行性和应用前景     

一氧化氮对犬下食管括约肌压力的作用

目的 :应用苄基二甲基十四烷氯化铵 (benzyldim ethyltetradecyl amm onium chloride,BAC)建立犬下食管括约肌(L ES)无神经动物模型 ,研究一氧化氮 (NO)对 L ES压力的作用。方法 :将 BAC环周注入犬 L ES,对照组注入等量生理盐水 ,均于注射前及注射后 6周测定 L ES压力 ;并观察 L -精氨酸、D -精氨酸、硝普钠及一氧化氮合酶 (NOS)抑制剂 N-硝基 - L -精氨酸 (L - NNA)对 L ES压力的影响 ;此外还测定了两组犬 L ES中 NO含量和 NOS活性。结果 :BAC处理组 L ES压力 [(4 2 .43±4.19) m m Hg,1m m Hg=0 .133 k Pa]显著高于对照组 [(2 2 .71± 5 .19) mm Hg]。 L -精氨酸可使对照组 L ES压力降低 ;L - NNA使其增高 ,但对 BAC处理组 L ES压力均无影响。硝普钠可降低两组犬 L ES压力。对照组 L ES中 NO为 (6 .0 5 8± 2 .0 6 7)μm ol/g,NOS为 (1.45 8± 0 .146 ) U /mg;而 BAC处理组 NO为 (1.797± 0 .873)μmol/g,NOS为 (0 .46 3± 0 .0 39) U /m g,均较对照组显著降低 (P<0 .0 1)。 结论 :BAC可使犬 L ES压力增高 ,其机制可能与 L ES局部 NO减少有关。     

积极胰岛素治疗对2型糖尿病患者胰岛功能恢复和一氧化氮水平的影响

目的　观察短期积极胰岛素治疗控制高血糖前后 2型糖尿病病人内源性胰岛素 (Ins)水平和血清一氧化氮 (NO)水平的动态变化。方法　 2型糖尿病无并发症者 2 0例 ,均为口服降糖药治疗 ,空腹血糖 >10 m mol/ L者。短期积极给与三餐前皮下注射胰岛素治疗 ,高血糖控制前后均作 OGTT及 Ins释放试验并测血清 NO水平。结果　 1治疗后空腹血糖水平和 OGTT血糖曲线下面积均较治疗前显著降低 ,分别为 (7.4 2± 1.71) m mol/ L vs(13.10± 2 .5 0 ) mm ol/ L,P=0 .0 0 0和 (4 5 .5 9± 2 1.34) mm ol/ (L· h) vs(6 1.10± 9.2 2 ) mm ol/ (L· h) ,P=0 .0 0 3;2治疗后空腹 Ins水平和 Ins曲线下面积较治疗前显著升高 ,分别为 (16 .0 3± 3.33) m U/ L vs(11.73±1.89) m U/ L,P=0 .0 0 0 ,和 (98.82± 33.2 2 ) m U/ (L· h) vs(5 7.83± 11.6 2 ) m U/ (L· h) ,P=0 .0 0 0 ;3治疗后血清 NO水平较治疗前显著升高 ,为 (12 6 .6 1± 75 .0 5 ) μmol/ L vs. (6 2 .0 7± 34.4 1) μmol/ L,P=0 .0 0 0。结论　葡萄糖毒性是抑制 2型糖尿病病人胰岛分泌功能和抑制 NO生成的环节之一 ,短期积极有效胰岛素控制高血糖有利于2型糖尿病病人胰岛分泌功能和 NO水平的恢复     

凹凸棒石粉对胆汁的吸附作用

目的 :探索降低反流胆汁中胆酸浓度的有效国产药物。方法 :以凹凸棒石粉或双八面体蒙脱石 (思密达 )吸附胆汁反流症患者胃液 ,检测并比较吸附前后胃液总胆酸和三羟胆酸含量 ,同时比较凹凸棒石粉剂和双八面体蒙脱石对胆酸的吸附能力。结果 :吸附前三羟胆酸和总胆酸含量分别为 (10 5 .71± 43.35 )μg/ ml和 (2 71.5 0± 77.5 0 )μmol/ L ,经凹凸棒石粉或双八面体蒙脱石吸附后三羟胆酸含量分别为 (32 .32± 18.36 )μg/ m l和 (2 9.13± 17.0 2 )μg/ ml,总胆酸含量分别为 (130 .0 1± 30 .94)μm ol / L与 (76 .90± 41.80 )μm ol/ L ,吸附前后三羟胆酸和总胆酸含量有显著差异 (P<0 .0 1) ,而经凹凸棒石粉或双八面体蒙脱石吸附后三羟胆酸和总胆酸含量无显著差异 (P>0 .0 5 )。 结论 :凹凸棒石粉对胆酸有较强的吸附作用 ,与双八面体蒙脱石无显著差异 ,有良好的临床应用前景。     

中老年女性精神分裂症患者血浆一氧化氮检测及临床意义

目的探讨血浆一氧化氮(NO)与中老年女性精神分裂症之间的关系.方法运用硝酸还原酶法对27例中老年精神分裂症患者的血浆NO含量进行检测,并与正常对照组比较.结果中老年女性精神分裂症患者的血浆NO含量(45.38±25.67)μmol/L高于正常对照组(33.44±15.55)μmmol/L,但无显著性意义(t=1.842,P＞0.05);以阳性症状为主的患者血浆NO含量为(49.95±31.18)μmmol/L,以阴性症状为主的患者为(40.04±16.26)μmmo1/L,均高于正常对照组,但仅前者与正常对照组差异有显著性(t=2.059,P＜0.05);阳性症状组和阴性症状组间差异无显著性(t=0.996,P＞0.05).血浆NO的含量与年龄、病程、家族史等无关.结论NO可能在中老年女性精神分裂症发病机制中起一定作用.     

川芎嗪对大鼠急性坏死性胰腺炎微循环与炎症介质的影响

目的:探讨急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)时肠组织血流量及炎症介质变化对肠损伤的影响及川芎嗪的保护作用。方法:SD大鼠96只,随机分为对照组(C组)、胰腺炎组(P组)和治疗组(T组)。以5%牛磺胆酸钠胰腺被膜下均匀注射复制ANP模型。采用放射性生物微球技术在制模后2 h及12 h分别测定肠组织血流量,同时检测血清磷脂酶A2(PLA2)活性及白细胞介素-1β(IL-1β)水平,并观察肠黏膜病理改变。结果:P组在制模后2h及12 h肠组织血流量(0.80±0.07,0.50±0.06)m l.(m in.g)-1较C组(1.56±0.18,1.61±0.11)m l.(m in.g)-1明显减少(P<0.001),血清PLA2活性(94.29±9.96,103.71±14.40)U/L,IL-1β水平(0.78±0.13,0.83±0.20)μg/L较C组(65.27±10.52,66.63±9.81)U/L,(0.32±0.06,0.33±0.07)μg/L明显升高(P<0.001),肠黏膜损伤程度较C组明显加重(P<0.001)。与P组比较,T组在制模后2h及12h,肠组织血流量(1.05±0.12,0.92±0.08)m l.(m in.g)-1较P组明显增高(P<0.01),血清PLA2活性(81.77±8.95,84.02±9.01)U/L,IL-1β水平(0.54±0.07,0.48±0.05)较P组明显降低(P<0.05),肠黏膜组织损伤程度较P组明显减轻(P<0.05)。结论:ANP早期肠组织血流量减少与炎症介质的升高同时发生,两者均是ANP时肠黏膜损伤的重要因素,川芎嗪对ANP肠损伤具有保护作用。     

体外反搏对血浆NO和内皮素-1水平的影响

增强型体外反搏(EECP)可以显著增加舒张期血流,被推测可以通过增加剪应力来改善内皮细胞功能。作者检验了EECP对血浆NO和内皮素-1(ET-1)水平的作用。对接受每天1h EECP治疗6周以上的13例冠状动脉疾病患者连续测定了血浆硝酸盐及亚硝酸盐(N Ox)和ET-1水平。在EECP治疗过程中,血浆NOx水平进行性升高,而血浆ET-1水平进行性降低。EECP治疗36h后,与基线水平比较,血浆NOx升高了62%±17%(43.6±4.3μm ol/L vs27.1±2.6μm ol/L,P<0.0001),ET-1降低了36%±8%(76.7±9.5pg/L vs119.5±8.5pg/L,P<0.0001)。在EECP治疗结束后3个月,…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.