西藏小型猪F1代与F0代生长繁殖性能的比较

目的比较研究广州地区西藏小型猪F1代与F0代引入群母猪繁殖和仔猪生长性能的差异。方法观察记录西藏小型猪F1代和F0代的产仔数、产活仔数、离乳仔数、初生重、30日龄和60日龄体重。结果西藏小型猪F1代和F0代母猪其初产平均产仔数、产活仔数、离乳仔数、平均初生重、30日龄和60日龄体重分别为3.83头、2.30头;3.29头、2.20头;3.14头、3.11头;0.66 kg、0.53 kg;2.70 kg2、.30 kg;4.50 kg、4.01 kg;F1代初产仔数和离乳率均显著高于F0代。结论西藏小型猪已经适应了广州高温高湿环境,能够稳定地生长繁殖。     

融水小型猪F1代生长研究

目的 测定融水小型猪F1代体重和体尺。方法 选取F1代融水小型猪83头（雌性48头,雄性35头）,测定初生至12月龄的体重、体长、体高、胸围、胸宽、胸深、管围、腿围、嘴裂长度共9个生长发育指标,并应用SPSS统计软件和Logistic非线性生长模型进行分析。结果 融水小型猪F1代的初生体重雌雄分别为0.61±0.14 Kg和0.55±0.13 Kg,6月龄体重雌雄分别为17.21±5.20 Kg和16.35±5.23 Kg,12月龄体重雌雄分别为26.97±6.49 Kg和26.53±5.65 Kg。雌雄比较,9项指标所测结果接近,除了初生体重和体长、10月龄胸宽有差异（P<0.05）,其余指标同月龄雌雄之间均无明显差异。应用Logistic模型分析,体重生长拐点在5～6月龄间,体长和腿围生长拐点在2～3月龄间,体高、胸围、胸宽、胸深、管围和嘴裂长度的生长拐点在1～2月龄间。结论 融水小型猪F1代成年体重轻,性情温顺,具备培养成实验用小型猪基本条件。     

版纳微型猪的生物学特性

版纳微型猪原产于云南省西双版纳州,属华南型猪种。为了发掘和利用本省小型猪资源,1987年5月从西双版纳州属3个县的原始产区引进了8头公猪和42头母猪到州种猪场饲养和选育。3年来对微型猪的繁殖性能、生长发育、生理生化、遗传特征、适应性和采食量等10个方面近150个性状参数进行了测定和分析,结果表明6月龄公猪的体重、体高和体长分别为13.10±0.40kg、32.34±0.36cm和54.59±0.79cm,母猪分别为17.21±0.42kg、34.40±0.24cm和58.52±0.60cm;成年公猪体重为36.12±1.09kg,母猪为43.29±1.04kg。微型猪性成熟早,4～6月龄即可配种繁殖,经产母猪平均每窝产活仔7.84±0.39头。同时,还测定了不同年龄阶段用作实验的离体部位、器官和腺体的重量,为微型猪的实验应用提供基本参数。3年来选育结果表明,微型猪的体型矮小和生长缓慢特点能稳定遗传,与体型有关的性状遗传力达到中等或中等以上,性状间也存在中等的遗传相关(r_A(xy)为0.35～0.64),13个胸椎和5个腰椎的个体频率分别为32.14％和21.43％。     

融水小型猪的实验动物化系列研究概述

融水小型猪的种源来自广西融水县,2012年被引到广东繁育并测定了基础数据,包括种质特性、繁殖性能、生长曲线、血液学指标、血生化指标、脏器系数、染色体分析。参照国家和地方有关实验动物标准,初步建立了融水小型猪微生物与寄生虫、环境与设施、饲料、病理学、遗传学等质量控制标准。融水小型猪能适应当地气候,繁殖良好,自然受孕率为88.3%,妊娠期平均112 d,初产平均胎产仔6.1头,经产平均产仔7.9头。融水小型猪体型小,雌雄成年体重(6月龄)分别为17.21±5.20 kg和16.35±5.23 kg,性情温顺,线粒体DNA分析表明融水小型猪比兰屿猪更为古老。通过标准化的饲养管理与质量控制,具有培育成实验用小型猪的基本条件。     

西藏小型猪ESR基因PvuⅡ多态性与生长繁殖性能相关分析

目的了解ESR基因PvuⅡ位点在西藏小型猪中的多态性分布情况;研究其在西藏小型猪上的遗传效应,为西藏小型猪生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法记录44头西藏小型猪母猪繁殖性能,并对其所产仔猪的生长性能进行测定,采用PCR-RFLP方法对ESR基因PvuⅡ位点进行多态性与生长繁殖性能进行相关性分析。结果西藏小型猪ESR候选基因PvuⅡ位点表现出多态性,为AA、A8、BB型,A、B等位基因频率特征。初产胎的产仔数在不同基因型间差异存在显著性,不同基因型间产仔数呈BB〉AB〉AA的趋势,初产胎和经产胎的母猪其他指标在不同基因型间差异均没有显著性。结论提高西藏小型猪猪群ESR基因PvuⅡ位点B等位基因频率,有利于提高西藏小型母猪的产仔数。     

两种小型猪代谢综合征相关指标比较研究

目的 探讨两种小型猪作为代谢综合征模型动物的可行性。方法 选择巴马小型猪和西藏小型猪,测量体重、体尺以及与代谢综合征有关指标-血脂、血糖、自由基水平,在不同种和性别之间进行比较分析。结果 与西藏小型猪比较,巴马小型猪体重大,体长度短,颈围大,腹围大,肥胖指数高,而且差异在雌性巴马小型猪中更加显著。TC、HDL-C、LDL-C、TC水平在雌性小型猪高于雄性小型猪。GLU水平在巴马小型猪高于西藏小型猪,并与雌性西藏小型猪的差异显著,而FRA和HbAlc水平在西藏小型猪高于巴马小型猪,Insulin水平在雌性巴马小型猪和雄性西藏小型猪中分别高于雄性巴马小型猪和雌性西藏小型猪。MDA水平在雌性巴马小型猪中显著高于雄性巴马小型猪和西藏小型猪,SOD和GSH-Px水平在巴马小型猪中高于西藏小型猪。结论 通过体重、体尺、血脂、血糖和自由基水平等正常生理指标的分析,巴马小型猪倾向于肥胖;两种小型猪血脂雌性高于雄性;巴马小型猪空腹血糖高于西藏小型猪,但巴马小型猪的血糖控制能力优于西藏小型猪;巴马小型猪抗氧化能力优于西藏小型猪。总之,在代谢综合征相关研究中根据具体需求进行选择不同种、不同性别的小型猪并以具体造模实践来加以验证。     

催乳素受体基因多态性对西藏小型猪繁殖性能的影响

目的了解催乳素受体（PRLR）基因AIuI位点在西藏小型猪中的多态性分布情况;研究其在西藏小型猪上的遗传效应,为生长繁殖性状的分子遗传标记辅助选择提供实验依据。方法收集44头西藏小型猪母猪窝产仔数及离乳仔数数据,测定所产仔猪的初生重和离乳重,采用PCR-RFLP方法测定PRLR基因AIuI位点多态性,并与上述生长繁殖性能指标进行相关性分析。结果西藏小型猪PRLR侯选基因AIuI位点表现出多态性,分为AA、AB、BB,频率分别为0.273、0.500、0.227。经产母猪的仔猪初生重在不同基因型间存在显著性差异,而初产和经产母猪的其他生长繁殖指标在不同基因型间均未达到显著性水平。结论本研究中PRLR基因AIuI位点多态性AA、AB、BB不同基因型之间窝产仔数和离乳仔数差异不显著。     

两种小型猪代谢综合征相关指标的比较

目的探讨两种小型猪作为代谢综合征模型动物的可行性。方法选择巴马小型猪和西藏小型猪,测量体重、体尺以及与代谢综合征有关指标-血脂、血糖、自由基水平,在不同种和性别之间进行比较分析。结果与西藏小型猪比较,巴马小型猪体重大,体长度短,颈围大,腹围大,肥胖指数高,而且差异在雌性巴马小型猪中更加显著。TC、HDL-C、LDL-C、TC水平在雌性小型猪高于雄性小型猪。GLU水平在巴马小型猪高于西藏小型猪,并与雌性西藏小型猪的差异显著,而FRA和Hb Alc水平在西藏小型猪高于巴马小型猪,Insulin水平在雌性巴马小型猪和雄性西藏小型猪中分别高于雄性巴马小型猪和雌性西藏小型猪。MDA水平在雌性巴马小型猪中显著高于雄性巴马小型猪和西藏小型猪,SOD和GSH-Px水平在巴马小型猪中高于西藏小型猪。结论通过体重、体尺、血脂、血糖和自由基水平等正常生理指标的分析,巴马小型猪倾向于肥胖;两种小型猪血脂雌性高于雄性;巴马小型猪空腹血糖高于西藏小型猪,但巴马小型猪的血糖控制能力优于西藏小型猪;巴马小型猪抗氧化能力优于西藏小型猪。总之,在代谢综合征相关研究中根据具体需求进行选择不同种、不同性别的小型猪并以具体造模实践来加以验证。     

钕铁硼磁体模拟脊柱后路不对称栓系对小型猪脊柱生长的影响——小型猪脊柱侧凸模型的构建

目的构建脊柱侧凸动物模型,探讨特发性脊柱侧凸病因学和发病机制,验证脊柱生长失衡假说。方法利用钕铁硼磁体的相互吸引力模拟后路经椎弓根不对称栓系相邻椎体,观察后路栓系对小型猪脊柱生长的形态学三维影响,提出导致脊柱发生畸形的可能阈值范围。16只1月龄雌性小型猪〔体质量为(7.54±0.46)kg〕分为对照组和实验组,每组8只。实验组于T8、T9、T10胸椎后路左侧经椎弓根植入直径为25 mm螺钉和磁体,保持相邻磁体间极性相吸和维持5 mm内距离。对照组仅植入螺钉。每隔4周复查脊柱正侧位片,直至12周。测量体质量、冠状位Cobb角、胸椎后凸角、顶椎(T9)两侧高度及椎体间磁力值的范围,采用完全随机设计量总体均数比较的t检验,确定2组的统计学差异。结果12周后小型猪平均体质量增加约2倍,实验组:(21.05±1.67)kg,对照组:(21.75±1.67)kg,P=0.769,2组差异无统计学意义。对照组小型猪1月龄时T9椎体高度为(6.5±0.60)mm,12周后为(13.06±1.37)mm,平均增加约1倍。实验组小型猪T8-T10的脊柱冠状位Cobb角为11.75°±2.87°,对照组为0.94°±0.86°,差异有统计学意义(P<0.000 1)。实验组矢状面出现胸椎前凸畸形,平均为-5.25°±6.21°,对照组为脊柱后凸,平均为4.25°±0.71°,差异有统计学意义(P=0.003)。结论钕铁硼磁体后路经椎弓根单侧不对称栓系小型猪T8-T10椎体,可以影响其脊柱的生长发育,并可以诱导出猪脊柱冠状面轻度的侧凸畸形和矢状面的前凸畸形。钕铁硼磁体对小型猪椎体生长板产生的栓系力约为1.0～2.3 N。本实验结果验证脊柱侧凸发病机制的脊柱生长失衡假说并且遵循Hueter-Volkmann法则,脊柱后路不对称栓系在脊柱侧凸畸形发生及进展中起重要作用,矢状面的前凸可能是脊柱侧凸的原发畸形。     

西藏小型猪10种猪病血清学调查

目的对西藏小型猪进行10种猪病(猪瘟、伪狂犬病、篮耳病、猪流感、圆环病毒感染、细小病毒病、乙型脑炎、弓形体病、衣原体病和布氏杆菌病)的血清学调查。方法采用ELISA或凝集试验方法对10头已注射猪瘟疫苗的原代(F0代)母猪和40头20日龄乳猪、50头第一代(F1代)3～5月龄猪进行10种猪病的血清学检测。结果猪瘟病毒、细小病毒和乙型脑炎病毒抗体阳性率分别是母猪为80.0%、80.0%、70.0%;20日龄乳猪为45.0%、25.0%、45.0%;3～5月龄猪为4.0%、76.0%、80.0%。圆环病毒抗体阳性率母猪为60.0%,20日龄乳猪为5.0%。3～5月龄猪为0%;篮耳病病毒、伪狂犬病病毒、猪流感病毒、弓形体、衣原体、布氏杆菌在不同年龄西藏小型猪中抗体阳性率均为0%。结论不同年龄西藏小型猪未受篮耳病病毒、伪狂犬病病毒、猪流感病毒、弓形体病、衣原体病、布氏杆菌病感染。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β,sIL-2R,IL-6,TNF-α,AND IFN-v IN ACUTE CORONARY SYNDROME

Objectives To explore serum cytokines levels (including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS.Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1 β, sIL-2R, IL-6, TNF-α, and IFN-v were measured by enzyme linked immunosorbent assay.Results Serum IL- 1 β, sIL-2R, IL-6, TNF-α were significantly higher in AMI group or UAP group compared to the control group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-v shows no significant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner's QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤ 6 scores group and ＞ 6 scores group.Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a useful marker reflecting disease stability.     

Possible Spread of adenovirus type 3 from poultry to humans ：indirect evidence from an outbreak in China

Objective:To explore the epidemiology and etiology for an outbreak of acute respiratory tract infection that occurred in one county of Jiangsu Province, China 2004. Methods: Only cases meeting the case definition were included in the study. We reviewed the medical records of the cases who were admitted to the local hospitals, interviewed cases by a standard questionnaire, and then described the epidemiotogic features and analyzed risk factors by means of a case-control study. We collected pharyngeal swab specimens and sent them to different laboratories for isolation and culture. The laboratory used different detection methods such as DIP, PCR, electron microscope examination and microneutralization assay, to identify and then type the positive specimens. Results:A total of 871 cases were reported during the period from April 18 to July 4,2004. The distribution of onset times presented two peaks, one in late May and another in middle June. The epidemic occurred mainly in the elementary and junior high schools in ten townships of one county, and the mean age of the cases was 12 years (range 7 months to 18 years). The course of the disease was acute, and was characterized by fever accompanied with sore throat and tonsillitis. The WBC count of cases was normal or elevated. The mean duration of illness was 5 days (range 2 to 12 days). No fatalities from illness were reported. A case-control study indicated that the possible risk factors were close contact with a case and/or poultry before onset and sharing of towels among members of the family. The typical CPE was observed through inoculating pharyngeal swab specimens into the HEP-2 cell cultures in different laboratories. An infection of adenovirus type 3 was verified by detecting positive specimens in different methods. Conclusion:This investigation demonstrated that the acute respiratory infection in cases was caused by adenovirus type 3. Cases occurred in over 70 schools in ten townships in 2004, and the route of transmission was possibly close contact with cases or droplet transmission.