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融水小型猪的实验动物化系列研究概述
引用本文:施赫赫,陈淦,刘科,刘运忠,王绪敏,高翔,贺争鸣,余细勇,赖良学,唐小江.融水小型猪的实验动物化系列研究概述[J].中国比较医学杂志,2015,25(3):86-89.
作者姓名:施赫赫  陈淦  刘科  刘运忠  王绪敏  高翔  贺争鸣  余细勇  赖良学  唐小江
作者单位:广东省医学实验动物中心, 广东 佛山 528248;广东贝格生物科技有限公司, 广东 佛山 528100;广东贝格生物科技有限公司, 广东 佛山 528100;广东省医学实验动物中心, 广东 佛山 528248;广州医药工业研究总院, 广州 510240;中国科学院北京基因研究所, 北京 100101;南京大学模式动物研究所, 南京 210061;中国食品药品检定研究院实验动物资源研究所, 北京 100050;广东省医学科学院, 广州 510060;中国科学院广州生物医药与健康研究院, 广州 510530;广东省医学实验动物中心, 广东 佛山 528248
基金项目:国家重大研究计划项目(2011CB944101);国家自然科学基金重点项目(81120108003;81330007)。
摘    要:融水小型猪的种源来自广西融水县,2012年被引到广东繁育并测定了基础数据,包括种质特性、繁殖性能、生长曲线、血液学指标、血生化指标、脏器系数、染色体分析。参照国家和地方有关实验动物标准,初步建立了融水小型猪微生物与寄生虫、环境与设施、饲料、病理学、遗传学等质量控制标准。融水小型猪能适应当地气候,繁殖良好,自然受孕率为88.3%,妊娠期平均112 d,初产平均胎产仔6.1头,经产平均产仔7.9头。融水小型猪体型小,雌雄成年体重(6月龄)分别为17.21±5.20 kg和16.35±5.23 kg,性情温顺,线粒体DNA分析表明融水小型猪比兰屿猪更为古老。通过标准化的饲养管理与质量控制,具有培育成实验用小型猪的基本条件。

关 键 词:融水小型猪  种质特性  实验动物化  质量控制
修稿时间:3/4/2015 12:00:00 AM

Brief report of series study on Rongshui miniature pig as laboratory animal
SHI He-he,CHEN Gan,LIU Ke,LIU Yun-zhong,WANG Xu-min,GAO Xiang,HE Zheng-ming,YU Xi-yong,LAI Liang-xue and TANG Xiao-jiang.Brief report of series study on Rongshui miniature pig as laboratory animal[J].Chinese Journal of Comparative Medicine,2015,25(3):86-89.
Authors:SHI He-he  CHEN Gan  LIU Ke  LIU Yun-zhong  WANG Xu-min  GAO Xiang  HE Zheng-ming  YU Xi-yong  LAI Liang-xue and TANG Xiao-jiang
Institution:Guangdong Medical Laboratory Animal Center, Guangdong Foshan, 528248, China;Beige BioTec Co., Ltd., Guangdong Foshan 528100, China;Beige BioTec Co., Ltd., Guangdong Foshan 528100, China;Guangdong Medical Laboratory Animal Center, Guangdong Foshan, 528248, China;Guangzhou Pharmaceutical Industry Research Institute, Guangzhou, 510240, China;Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China;Model Animal Research Center of Nanjing University, Nanjing 210061, China;Chinese Experimental Animal Resources Research Institute for Food and Drug Control, Beijing 100050, China;Guangdong Academy of Medical Sciences, Guangzhou 510060, China;Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China;Guangdong Medical Laboratory Animal Center, Guangdong Foshan, 528248, China
Abstract:The provenances of Rongshui miniature pigs (RMPs) were purchased from Rongshui, Guangxi, in 2012. 130 RMPs were transported to Sanshui, Guangdong,China, which were breed according to the laboratory animal standards. 83 RMPs were selected randomly from the first filial generations (F1). The basic data were collected including breed characteristics, reproductive performance, grow curve, hematology, biochemical markers of serum and urine, organ coefficient, Chromosome analysis. According to the national and local standards, the quality control standards of RMP were set up including microbiological, parasitic, environment and facilities, fodder, pathology, genetic. The results showed that RMPs adapt to the climate of Guangdong. The natural mating and conceive rate was 88.3% with the pregnancy of 112 days. The average number of firstborn and still-born was 6.1 and 7.9 respectively. RMP was small body size with the adult body weight of 17.21±5.20 kg and 16.35±5.23 kg in female and male respectively. RMP was very tame. The mitochondrial genome analysis suggested RMP belonged a typical miniature pig breed in China, which is ancient than Lanyu pig. RMP could be breed as a new kind of laboratory animal.
Keywords:Rongshui miniature pig(RMP)  Characteristic  Experimental animalization  Quality control
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