中华鼢鼠骨提取物对炎症反应的影响

目的：观察中华鼢鼠骨提取物的药理作用。方法：采用FCA介导大鼠超敏反应性炎症，角叉菜胶致大鼠急性非特异性炎症，小鼠腹腔毛细血管通透性实验及醋酸所致的扭体实验。结果：中华鼢鼠骨提取物对由FCA介导的大鼠佐剂性关节炎早期炎症反应和继发病变均有明显抑制作用，能明显减轻局部炎症组织的病理损害，防止全身病变的发生。能明显抑制由角叉菜胶引起急性炎症大鼠的足肿胀，对HAC所致小鼠腹腔毛细血管通透性增加有明显抑制作用，明显减少HAC所致小鼠扭体反应次数。结论：中华鼢鼠骨提取物具有抗炎镇痛作用。     

加减补阳还五汤对佐剂性关节炎大鼠作用的实验研究

目的 研究加减补阳还五汤对大鼠佐剂性关节炎的影响,探讨加减补阳还五汤治疗类风湿性关节炎的作用机制.方法 建立大鼠足跖注射弗氏完全佐剂性关节炎模型,观察加减补阳还五汤对动物超敏反应性炎症血清中白细胞介素(IL-1)、肿瘤坏死因子(TNF-α)、循环免疫复合物(CIC)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性的影响.结果 加减补阳还五汤能降低模型动物的IL-1、TNF-α、CIC、MDA含量,提高SOD活性,均有显著性差异(P＜0.01).结论 加减补阳还五汤具有抗大鼠佐剂性、继发性关节炎作用,其机制与抑制自由基损伤和降低自身免疫的病理生理反应有关.     

塞隆骨提取物对CFA诱导活化的小鼠腹腔巨噬细胞功能的影响

目的:研究塞隆骨提取物对弗氏完全佐剂诱导活化的腹腔巨噬细胞的免疫抑制作用.方法:小鼠腹腔注射弗氏完全佐剂诱导腹腔巨噬细胞活化并回收,体外加入LPS及IFN-γ刺激培养巨噬细胞,利用酶联免疫法测定培养上清中细胞因子(IL-1β、IL-6、IL-12p40、TNF-α)水平.结果:塞隆骨水提物及90%醇沉部分体内给药均能够能显著抑制弗氏完全佐剂诱导的巨噬细胞向腹腔的浸润,能抑制巨噬细胞在LPS及IFN-γ刺激下生成细胞因子(IL-12p40、IL-1β、IL-6、TNF-α).结论:塞隆骨提取物对巨噬细胞功能有一定程度的影响,能够抑制其产生炎症因子,可能是其治疗类风湿性关节炎的作用机理之一.     

伸筋草正丁醇提取物对佐剂性关节炎大鼠血清IL-1β、IL-6、TNF-α的影响

目的:研究伸筋草正丁醇提取物对佐剂性关节炎(Adjuvant Arthritis,AA)大鼠血清中细胞因子IL-1β、IL-6和TNF-α的影响,探讨其治疗类风湿性关节炎(RA)的作用及免疫学机制。方法:采用弗氏完全佐剂(CFA)诱导大鼠AA模型,给予不同剂量的伸筋草正丁醇提取物治疗,应用放射性免疫法,检测血清中细胞因子系列(IL-1β、IL-6、TNF-α)的水平。结果:模型组大鼠血清IL-1β、TNF-α、IL-6水平均高于正常组(P<0.01);各治疗组IL-1β、IL-6和TNF-α水平均有显著降低(P<0.01或P<0.05)。结论:伸筋草正丁醇提取物可能是通过免疫调节机制,对佐剂性关节炎发挥治疗作用。     

迷迭香酸抗炎作用研究

目的：研究迷迭香酸的抗炎作用。方法：采用鼠耳肿胀法、腹腔毛细血管通透性法等急性炎症模型、慢性肉芽肿模型及大鼠佐剂性关节炎模型观察迷送香酸对急、慢性炎症和免疫性炎症的抗炎作用。结果：迷迭香酸能明显抑制二甲苯所致小鼠耳肿胀、醋酸所致小鼠毛细血管通透性增高及大鼠棉球肉芽肿性炎症，对Freund＇s完全佐剂介导的大鼠佐剂性关节炎足肿胀的抑制作用强弱与剂量大小呈较好的量效关系。另外，迷迭香酸能降低炎症模型动物血清中炎症细胞因子TNF-α、IL-β、CRP的含量。结论：迷迭香酸有明显的抗炎和免疫抑制作用，降低炎性细胞因子TNF-α、IL-1β、CRP的产生可能是迷迭香酸减轻炎症反应机制之一。     

栀子总苷对大鼠佐剂性关节炎治疗作用及部分机制的研究

目的:观察栀子总苷(TGCJ)对大鼠佐剂性关节炎的治疗作用和对炎症区域一氧化氮(NO)、一氧化氮合成酶(NOS)及相关炎症介质的影响,探讨其治疗类风湿关节炎(RA)的作用机制。方法:弗氏完全佐剂(FCA)免疫诱导大鼠佐剂性关节炎(adjuvantarthritis,AA);SD大鼠随机分为6组:正常组、模型组、TGCJ3个剂量组(20,40,80mg·kg^-1)和雷公藤多苷(GTW)组;足爪容积测定法和关节炎评分法观察关节炎的发生情况;测定大鼠关节滑膜组织中NO、NOS、前列腺素E₂(PGE₂)及白细胞介素-1β(IL-1β)、肿瘤坏死因子(TNF-α)水平。结果:与正常组比较,模型组大鼠关节滑膜组织NO、NOS、PGE₂I、L-1β和TNF-α水平显著升高;与模型组相比,TGCJ组大鼠佐剂性关节炎继发病变显著受抑制,关节滑膜组织NO、NOS、PGE₂及炎性细胞因子IL-1β和TNF-α水平显著降低。结论:抑制关节炎炎症局部炎性细胞因子活性和降低炎症介质含量是栀子总苷发挥治疗类风湿关节炎作用的重要机制之一。     

黑骨藤抗类风湿性关节炎作用及其分子机制

目的:研究黑骨藤对类风湿性关节炎(rheumatoid arthritis,RA)的防治作用及其作用的分子机制。方法:将Wister雄性大鼠70只分成7组,sc Freund’s完全佐剂(空白组除外)建立佐剂性关节炎(adjuvant arthritis,AA)动物模型,观察黑骨藤提取物低、中、高剂量(20,50,100 mg·kg-1)对AA大鼠足肿胀、体重、器官指数及血清与炎性组织液中白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子α(TNF-α)含量水平的影响。结果:黑骨藤能有效抑制AA大鼠足肿胀,降低胸腺指数,下调AA大鼠血清与炎性组织液中IL-1β,IL-6,TNF-α的含量水平。造模第12 d后,黑骨藤高、中剂量组较模型组大鼠足跖肿胀度有极显著差异(P<0.01);模型组胸腺指数平均为1.50%,黑骨藤高剂量组为1.12%,两者差异极显著(P<0.01);黑骨藤高剂量组AA大鼠血清与炎性组织液中IL-1β,IL-6和TNF-α3项指标与模型组比较差异均显著(P<0.05)。结论:黑骨藤具有良好的抗RA作用,其作用机制可能与调节免疫器官功能及促炎细胞因子IL-1β,IL-6,TNF-α的含量水平有关。     

京尼平苷对佐剂性关节炎大鼠血清TNF-α、IL-6的影响

目的:通过观察京尼平苷对佐剂性关节炎大鼠血清IL-6和TNF-α的影响,探讨京尼平苷治疗类风湿性关节炎(Rheumatoid Arthritis,RA)的可能机制。方法:采用弗氏完全佐剂(Freund's Complete Adjuvant, FCA)复制出大鼠佐剂性关节炎(Adjuvant Arthritis,AA)模型,观察京尼平苷对实验性大鼠足肿胀的抑制作用;检测血清鼠抗人肿瘤坏死因子(TNF-α)、白介素-6(Interleukin-6,IL-6)的水平。结果:与模型组比较,京尼平苷(Geniposide, GP)可以延缓对侧肢体足肿胀发生的时间,抑制大鼠足肿胀的程度(P 0.05);用药后,给药组血清IL-6和TNF-α水平明显低于模型组(P 0.01)。结论:京尼平苷在类风湿性关节炎大鼠中可以下调致炎因子IL-6和TNF-α的水平,阻止AA大鼠免疫性炎症的发展,提示这可能是其治疗实验性类风湿性关节炎病情进展的可能机制。     

高乌甲素抑制佐剂性关节炎大鼠模型的实验研究

目的:研究高乌甲素对大鼠佐剂性关节炎模型的影响.方法:将大鼠随机分为空白对照组、模型组,地塞米松阳性对照组、高乌甲素高、中、低剂量组.将大鼠足跖注射Freund's完全佐剂形成佐剂性关节炎(AA),观察各组大鼠炎症左右足跖厚度、血清肿瘤坏死因子(TNF-α)、白细胞介素-2(IL-2)和循环免疫复合物(CIC)的含量变化.结果:与模型组比较,注射佐剂的大鼠右后跖从第2天开始肿胀持续15 d(原发性损害),高乌甲素可抑制大鼠足肿胀程度,能降低大鼠血清TNF-α、IL-2、CIC和PGEa含量而发挥抗炎作用.结论:高乌甲素及地塞米松具有抗佐剂性关节炎(AA)及降低致炎因子和相关炎症介质的作用.     

雷公藤双层片对系统性红斑狼疮治疗作用及机制的实验研究

目的：探讨雷公藤双层片治疗系统性红斑狼疮(SLE)的作用及机制，为临床应用提供实验依据。方法：采用兔Arthus反应，大鼠佐剂性关节炎模型进行雷公藤双层片治疗SLE的实验研究。结果：雷公藤双层片对家兔Arthus反应有明显的抑制作用。使Arthus反应家兔循环免疫复合物(CIC)含量以及血清IL-2水平明显下降。雷公藤双层片明显抑制大鼠佐剂性关节炎的继发病变，使佐剂性关节炎大鼠脾重量减轻，肾上腺重量增加。血浆皮质醇水平升高。结论：雷公藤双层片对SLE有明显的治疗作用，其机制可能与它降低血清IL-2水平。抑制Th细胞和B细胞功能，导致循环免疫复合物减少有关，亦与其通过类激素样抗炎作用，减轻机体炎症反应有关。     

穿山龙总皂苷对佐剂性关节炎大鼠免疫调节作用的实验研究

目的:研究穿山龙总皂苷对佐剂性关节炎大鼠(AA)的免疫调节作用。方法:将大鼠随机分为对照组、阳性药物组和实验组。连续灌胃给药8天,停药后处死大鼠,计算胸腺指数、脾指数,观察血清和关节液IL-1、IL-6、IL-8、TNF-α水平,观察ConA诱导的脾细胞增殖反应。结果:不同剂量穿山龙总皂苷治疗能使AA大鼠降低的胸腺指数基本恢复到正常,也能使AA大鼠明显增大的脾指数显著降低。高、中、低剂量组给药大鼠的血清、关节液内炎性细胞因子IL-1、IL-6、IL-8、TNF-α水平较AA模型组明显降低(P<0.01),ConA诱导的脾细胞增殖反应明显提高。结论:穿山龙总皂苷有良好的抗炎及免疫调节作用,其机理部分可能与调节机能依赖性的双向免疫及抑制炎性细胞因子产生有关。     

Therapeutic effects and mechanisms of total flavonoids of Turpinia Arguta Seen on adjuvant arthritis in rats

AIM: To investigate the therapeutic effects and mechanisms of total flavonoids of Turpinia Arguta Seen (TFS) on adjuvant arthritis in rats. METHODS: The model of adjuvant arthritis was induced by injection of Freund's Complete Adjuvant (FCA). Secondary paw swelling of AA rats was measured with volume meter and polyarthritis index were scored. The splenocyte proliferation, (interleukin-1) IL-1 and interleukin-2 (IL-2) production were assayed by cell proliferation assay. Prostaglandin E(2) (PGE(2)) production was determined by radioimmunoassay. RESULTS: TFS (80, 160, 320 mg/kg, i.g.) could significantly inhibit secondary inflammatory reaction (secondary swelling, multiple arthritis, pathologic change of ankle arthritis) in AA rats. The results in vivo showed that the low response of splenocytes to concanavalin A (Con A) and lipopolysaccharide (LPS) and the decreased IL-2 synthesis were restored in AA rats treated with TFS (160, 320 mg/kg, i.g.), while the elevated IL-1 and PGE(2) released from peritoneal macrophages (PMphi) were also reduced. CONCLUSION: TFS has significant therapeutic effect on AA rats, which might be relate to its immunoregulatory actions.     

马钱子总碱囊泡凝胶对AA大鼠的治疗作用

目的:观察马钱子总碱囊泡凝胶对佐剂性关节炎(AA)大鼠的治疗作用及其机制。方法:SD大鼠随机分为9组:正常组、AA模型组、马钱子总碱囊泡凝胶高、中、低剂量(25,12.5,6.25 mg·kg-1)组、马钱子总碱药物凝胶高、中、低剂量(25,12.5,6.25 mg·kg-1)组和扶他林对照组(双氯芬酸二乙胺乳胶剂,50 mg·kg-1)。除正常组外,其余各组均以弗氏完全佐剂(FCA)每鼠左后足跖皮内注射0.1 mL致炎,各给药组在大鼠足趾部分均匀涂抹药物并用油纸包裹。足容积法测量继发侧足肿胀度,进行疼痛评分和多发性关节炎评分,HE染色观察关节病理损伤,酶联免疫吸附测定法(ELISA)测定白细胞介素1(IL-1)、肿瘤坏死因子α(TNF-α)、前列腺素(PGE2)、白细胞介素6(IL-6)和血管内皮细胞生长因子(VEGF)的表达。结果:与模型组相比,囊泡凝胶和药物凝胶可明显减轻AA大鼠的继发侧足爪肿胀度(17～21 d)。马钱子总碱囊泡凝胶和药物凝胶可有效抑制大鼠多发性关节炎指数,减轻关节病理学损伤,马钱子总碱囊泡凝胶还可降低滑膜组织匀浆上清液IL-1,TNF-α,PGE2,IL-6水平和提高VEGF水平。结论:马钱子总碱囊泡凝胶可拮抗大鼠AA发展,其作用机制与抑制炎症介质的生成密切相关。     

Effects of ethanol and water extracts of propolis (bee glue) on acute inflammatory animal models

The anti-inflammatory effects of ethanol (EEP) and water (WSD) extracts in ICR mice and Wistar rats were analyzed. Both WSD and EEP exhibited significant anti-inflammatory effects in animal models with respect to thoracic capillary vessel leakage in mice, carrageenan-induced oedema, carrageenan-induced pleurisy, acute lung damage in rats. The mechanisms for the anti-inflammatory effects probably involve decreasing prostaglandin-E₂ (PGE₂) and nitric oxide (NO) levels. In rats with Freund's complete adjuvant (FCA) induced arthritis, propolis extracts significantly inhibited the increase of interleukin-6 (IL-6) in inflamed tissues, but had no significant effect on levels of interleukin-2 (IL-2) and interferon-γ (IFN-γ). The results are consistent with the interpretation that EEP and WSD may exert these effects by inhibiting the activation and differentiation of mononuclear macrophages.     

Protective effect of latex of Calotropis procera in Freund's Complete Adjuvant induced monoarthritis

The protective effect of latex of Calotropis procera in Freund's Complete Adjuvant (FCA) induced monoarticular arthritis was evaluated in rats. Arthritis was induced by a single intra‐articular injection of 0.1 mL of 0.1% FCA in the right ankle joint. The effect of dried latex (DL, 200 and 400 mg/kg) and its methanol extract (MeDL, 50 and 500 mg/kg) following oral administration was evaluated on joint inflammation, hyperalgesia, locomotor function and histology at the time of peak inflammation. The effects of DL and MeDL were compared with antiinflammatory drugs phenylbutazone (100 mg/kg), prednisolone (20 mg/kg), rofecoxib (20 and 100 mg/kg) and immuno‐suppressant methotrexate (0.3 mg/kg). Daily oral administration of DL and its methanol extract (MeDL) produced a significant reduction in joint inflammation (about 50% and 80% inhibition) and associated hyperalgesia. The antihyperalgesic effect of MeDL was comparable to that of rofecoxib. Both DL and MeDL produced a marked improvement in the motility and stair climbing ability of the rats. The histological analysis of the arthritic joint also revealed significant reduction in oedema and cellular infiltration by MeDL that was comparable to that of rofecoxib. Thus, our study suggests that the latex of C. procera has the potential to be used as an antiarthritic agent. Copyright © 2008 John Wiley & Sons, Ltd.     

Berberis crataegina DC. root exhibits potent anti-inflammatory, analgesic and febrifuge effects in mice and rats.

Extracts obtained from the roots and barks of various Berberis species are used as folk remedy worldwide for the treatment of various inflammatory ailments including lumbago, rheumatism and to reduce fever. Effects of the extracts and fractions from the roots of Berberis crataegina DC. (Berberidaceae) were studied using various in vivo models of inflammation in mice and rats and observed potent inhibitory activity against carrageenan- and serotonin-induced hind paw oedema, acetic acid-induced increased vascular permeability, castor oil-induced diarrhoea, and Freund's complete adjuvant-induced (FCA) arthritis models. Through bioassay-guided fractionation berberine was isolated as the main active ingredient. Moreover, a dose-dependent analgesic activity was determined, which assessed by using the model based on the inhibition of acetic acid-induced writhing reflexes, as well as antipyretic activity on FCA-induced increased body temperature. Acute and subchronic toxicity studies were also performed.     

艾炷灸贴预处理对佐剂性关节炎大鼠早期和继发期血清白细胞介素-6、睾酮、生长激素及皮质醇的影响

目的:探讨艾炷灸贴预处理对佐剂性关节炎(AA)大鼠早期与继发期血清炎性因子和激素的影响及保护机制。方法:将40只Wistar大鼠随机分为正常组、模型早期组和继发组、预灸贴早期组和继发组。于造模前用中药膜剂贴敷大鼠“大椎”穴后加艾炷灸,采用弗氏完全佐剂制备大鼠模型,用放射免疫法测定各组大鼠血清白细胞介素-6(IL-6)、睾酮(T)、生长激素(GH)及皮质醇(CORT)的含量。结果:血清IL-6和GH含量造模后均明显升高(P<0.01);与同期模型组相比,预灸贴继发组均明显下降(P<0.05)。血清T含量造模后早期组明显降低(P<0.05),继发组变化不大;预灸贴各组较同期模型组均显著升高(P<0.01,0.05)。血清CORT含量造模后显著升高(均P<0.01),而预灸贴继发组与正常组比无明显差异(P>0.05)。结论:艾炷灸贴预处理可减轻随后佐剂性关节炎大鼠原发足局部病理损伤,降低血清炎性因子IL-6的水平,对血清T、GH及CORT等内分泌激素的紊乱状态具有良性调节作用。     

结肠康Ⅰ号对溃疡性结肠炎大鼠血清IL-6、IL-10的影响

目的：探讨结肠康Ⅰ号对溃疡性结肠炎的修复作用及对血清IL-6、IL-10的影响。方法：SD大鼠60只,随机分为4组,分别为正常对照组、模型组、结肠康I号大、小剂量组。以5%2、4、6,三硝基苯磺酸（TNBs）诱导大鼠形成结肠炎症,实验完成后处死大鼠,观察大体形态学及组织病理学改变,并采用放射免疫法检测各组大鼠的血清IL-6、IL-10的水平。结果：结肠康Ⅰ号能有效提高大鼠血清IL-10含量,降低IL-6含量,并对损伤的大肠黏膜有明显的修复作用。结论：结肠康Ⅰ号对大鼠实验性溃疡性结肠炎疗效显著,其作用机制可能与调节机体的免疫有关。     

灵芝对佐剂性关节炎的作用

腹腔注射灵芝１ｇ／ｋｇ，可使佐剂性关节炎（ａｄｊｕｖａｎｔａｒｔｈｒｉｔｉｓ，ＡＡ）大鼠急性炎症和继发性炎症明显减轻，ＩＬ－２和ＩＬ－１的产生有一定恢复，淋巴细胞增殖反应低下状态有一定改善。     

Effects and mechanisms of total glucosides of paeony on synoviocytes activities in rat collagen-induced arthritis

The aim of the study was to investigate the effects of TGP, an active compound extracted from the roots of Paeonia lactiflora Pall, on the activities of synoviocytes in rats with collagen-induced arthritis (CIA) and its possible mechanisms. CIA was induced in male Sprague-Dawley (SD) rats immunized with chicken type II collagen (CII) in Freund's complete adjuvant (FCA). Synoviocytes proliferation was determined by 3-(4, 5-2dimethylthiazal-2yl) 2, 5-diphenyltetrazoliumbromide (MTT) assay. Tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), prostaglandin E(2) (PGE(2)) and cyclic adenosine monophosphate (cAMP) levels in synoviocytes were measured by radioimmunoassay (RIA). E-prostanoid (EP)(2) and EP(4) receptors were analyzed by Western blot analysis. The results showed that TGP significantly inhibited the proliferation of synoviocytes, decreased the production of IL-1, TNF-alpha and PGE(2) and elevated the levels of cAMP. Further study showed that TGP could up-regulate the expression of EP(2) and EP(4). These results indicated that TGP might exert its anti-inflammatory effects through inhibiting the production of pro-inflammatory mediators in synoviocytes of CIA rats, which might be associated with its ability to regulate cAMP-dependent EP(2)/EP(4)-mediated pathway.