猪囊尾蚴抗原cC1 DNA疫苗和蛋白质疫苗联合诱导小鼠免疫应答

目的:观察cC1 DNA疫苗和蛋白质疫苗联合免疫对诱导BALB/c小鼠免疫应答的影响.方法:雌性BALB/c小鼠随机分为4组:A组(DNA疫苗组), 质粒DNA以10 d间隔免疫3次;B组(联合免疫组),质粒DNA以10 d间隔免疫 2次后以GST-cC1融合蛋白加强免疫1次;C组(蛋白质疫苗组),分别在第0、3 周免疫接种融合蛋白;D组(pcDNA3对照组),以10 d间隔,3次免疫接种质粒pcDNA3.ELISA法检测血清样品中的特异性抗体水平以及实验小鼠脾脏淋巴细胞分泌IFN-γ和IL-4水平,以MTT法行脾脏淋巴细胞增殖实验.结果:C组可诱导相对较强的小鼠免疫应答,其特异性IgG和IgG1以及脾脏淋巴细胞分泌IL-4水平均高于其他各组(P<0.05),免疫的类型以Th2应答为主.B组诱导的特异性抗体水平和淋巴细胞分泌细胞因子水平均明显高于A组(P<0.05),B组和A组诱导的免疫应答类型均以Th1应答为主.结论:以 DNA priming-protein boost的策略可有效诱导较DNA疫苗单独使用更高的特异性抗体应答,且以Th1型免疫应答为主.     

肾癌G250 MN/CAIX重组质粒DNA免疫效应的初步研究

[目的]观察PCDNA3.0-G250重组质粒在小鼠中诱导产生DNA免疫应答的特点及抑瘤效应。[方法] PCDNA3.0-G250质粒每隔2周肌注射Balb/c小鼠共3次,对照组同法肌注PCDNA3.0质粒。每次免疫10 d后眶后取血,间接免疫荧光法检测血清抗体。6周后取脾细胞观察体外细胞毒效应。同法免疫Balb/c小鼠,第3次免疫时皮下种植sp2/0- PCDNA3.0-G250细胞,观察肿瘤大小及小鼠生存期。[结果]DNA免疫后可检测到特异性抗体,抗体滴度随免疫次数增加逐渐升高,差异有统计学意义(P<0.01)。体外CTL杀伤率与对照组差别有统计学意义。体内实验发现治疗组与对照组抑瘤效应无明显差异(P>0.05)。[结论]G250重组质粒DNA免疫能在小鼠巾诱导产生特异性细胞免疫及体液免疫,无明显体内抑瘤效应。     

细粒棘球绦虫Eg95重组蛋白诱导小鼠免疫应答的研究

目的:探讨细粒棘球绦虫Eg95重组蛋白诱导小鼠的免疫应答效果。方法:设立Eg95重组蛋白免疫组(rEg95组)和对照组(NS组)小鼠分别注射Eg95重组蛋白、福氏佐剂和生理盐水,收集各组血清用酶联免疫吸附(ELISA法)检测IgG水平;采集脾细胞用四甲基偶氮唑盐试验(MTT法)检测免疫小鼠的淋巴细胞增殖反应。结果:Eg95重组蛋白免疫小鼠在第2次免疫后开始检测到抗Eg95抗原的IgG,并随着免疫次数的增多,血清抗体效价升高,在第1次免疫后第10周时,免疫抗体滴度可达到1∶25600。在第4次免疫后,进行淋巴细胞转化实验,MTT法检测证实Eg95重组蛋白免疫的小鼠,其脾细胞可在体外被特异性刺激增生。结论:细粒棘球绦虫Eg95重组蛋白可诱发小鼠产生特异的体液免疫和细胞免疫应答。     

SARS病毒N蛋白真核表达质粒构建及诱导的体液免疫

目的构建SARS病毒核衣壳蛋白（N）的真核表达质粒，并研究其在小鼠体内诱导的体液免疫应答。方法采用PCR方法体外扩增SARS病毒N蛋白基因片段，定向克隆入真核表达栽体pVAC，构建pVAC-N重组质粒；大量制备该重组质粒，经基因枪腹部皮内注射免疫BALB／c小鼠三次，间接ELISA法测定免疫鼠IgG抗体效价。结果成功构建了重组表达质粒pVAC-N，免疫小鼠后可诱导产生出特异性的IgG抗体。结论构建的真核表达质粒pVAC-N能诱导小鼠产生特异性抗体，为SARS疫苗的进一步研究打下基础。     

全反式维甲酸对基因免疫应签的调节作用(摘要)

目的 研究全反式维甲酸(ATRA)对TR421-hCG8质粒基因免疫诱生的特异性细胞免疫与体液免疫应答的调节作用。方法 肌肉注射重组质粒TR421-hCGβ DNA(每只鼠50μ／100μl)初次免疫小鼠，以灌胃的方式给予ATRA，并以灌溶和TR421质粒免疫为对照；3周与6周后经同样的方式加强免疫各组小鼠，采用ELISA方法对基因免疫小鼠血清中IgG抗体水平进行动态观察，分析小鼠血清中IgG抗体亚类；3H-TdR掺入法测定特异性细胞增殖和细胞杀伤功能。结果 ELISA结果表明，TR421-hCGβ质粒基因免疫诱生较高的抗hCGβ抗体水平，ATRA增强TR421-hCG8质粒基因免疫诱生的抗hCGβ特异性IgG抗体水平并且伴随IgG2a／IgG1显著性降低；TR421-hCGβ质粒基因免疫诱生较强的淋巴细胞增殖活性和CTL活性，ATRA抑制TR421-hCGβ质粒基因免疫诱生的特异性细胞增殖和细胞杀伤功能。结论 ATRA促进基因免疫诱生的TH2免疫应答，抑制TH1型免疫应答，为改变基因免疫诱生的特异性免疫应答类型提供了一条新的途径。     

SFTSV糖蛋白Gn重组质粒的构建及其体液免疫原性研究

目的:研究严重发热伴血小板减少综合征病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)糖蛋白Gn的体液免疫原性?方法:将PCR方法扩增的SFTSV糖蛋白Gn基因和密码子优化后Gn基因克隆入载体pJW4303,构建Gn野生型和双优化重组质粒;经酶切和测序鉴定确认为目的质粒后,分别转染HEK293T细胞,Western blot检测糖蛋白Gn的表达;用Gn重组表达质粒及空载体分别免疫BALB/c小鼠,ELISA检测免疫后小鼠血清抗Gn特异性IgG抗体?结果:成功构建Gn重组野生型质粒pJW4303-WSP-Gn和双优化质粒pJW4303-tPA-Gn-opt;Western blot证实pJW4303-WSP-Gn编码Gn可在HEK293T细胞内表达,pJW4303-tPA-Gn-opt编码Gn在HEK293T细胞内表达并分泌到细胞外;ELISA检测免疫后小鼠血清抗Gn特异性IgG抗体证实各重组质粒均能诱导特异性IgG抗体产生,双优化质粒较野生型质粒诱导产生的IgG时间更早?滴度更高?结论:SFTSV的糖蛋白Gn具有良好的免疫原性;和野生型质粒相比,双优化质粒更有利于糖蛋白Gn的表达和分泌,并且具有更好的体液免疫原性?     

HBV preS2-S DNA免疫诱导小鼠体液免疫应答

目的:构建HBV表面抗原preS2-S基因表达质粒,并探讨其在小鼠体内的表达及诱导体液免疫应答的能力.方法:采用PCR方法,以PBR322-HBV2.0(adr亚型)质粒DNA为模板获得HBV preS2-S基因,并将其重组进入pcDNA3.0表达载体中,转染7721细胞系进行稳定表达;以此重组质粒免疫小鼠,ELISA方法检测免疫小鼠抗HBs抗体浓度.结果:构建了HBV preS2-S基因的表达质粒pcDNAS2-S,该表达质粒可在7721细胞中稳定高效表达;免疫接种小鼠2周后抗HBs抗体浓度明显升高,接种后第4周布比卡因处理组小鼠抗HBs抗体的浓度达到峰值161.4 mIU/ml,布比卡因非处理组第5周抗HBs抗体的浓度可达133.7 mIU/ml.结论:所构建的pcDNAS2-S表达质粒能有效表达并诱导小鼠体液免疫应答.     

结核杆菌多价核酸疫苗的构建及其免疫原性的初步研究

目的构建包含结核杆菌3种抗原蛋白Ag85A、ESAT-6和CFP-10真核表达质粒的多价DNA疫苗,并对其在小鼠体内的免疫原性进行初步分析。方法PCR扩增获得结核杆菌ag85 a、esat-6和cfp-10基因片段,分别克隆入真核表达质粒VR1020的BamHⅠ位点构建DNA疫苗,以此多价DNA疫苗免疫BALB/c小鼠,检测小鼠体内的特异性抗体应答和细胞免疫反应,并与BCG免疫组、质粒VR1020免疫组和生理盐水对照组相比较。结果多价DNA疫苗免疫小鼠后能产生特异性的细胞和体液免疫应答,DNA疫苗免疫组小鼠脾细胞IFN-γ的表达量显著高于BCG免疫组,而DNA疫苗免疫组小鼠所产生的特异性抗体水平低于BCG免疫组。结论成功构建了结核杆菌多价DNA疫苗,免疫小鼠后检测到特异性细胞和体液免疫应答。     

空肠弯曲菌外膜蛋白福氏佐剂疫苗免疫小鼠后的抗体应答

目的 探讨空肠弯曲菌甘氨酸提取物28～31 kd外膜蛋白福氏佐剂疫苗免疫小鼠后的抗体应答效果.方法 将30只BALB/c小鼠随机分为5组,每组6只,采用空肠弯曲菌甘氨酸提取物28～31 kd外膜蛋白以不同剂量疫苗组,分别在第0、7、14、21、28天,通过背部及腹部皮下多点注射免疫小鼠;空白组、对照组分别采用0.4 mL生理盐水(NS)、0.4 mL福氏完全佐剂.在末次免疫10 d(即第38天),分别应用双向免疫琼脂扩散试验法、试管凝集法检测血清特异性抗体效价, ELISA检测血清和肠液中的特异性IgG、IgA、.结果 末次免疫10 d后,各疫苗组中,双向免疫琼脂扩散试验法、试管凝集法检测血清特异性抗体效价分别达到1:4～1:16和1:320～1:1 280,ELISA法检测血清、肠液中IgG、IgA、的水平与空白组、对照组比较差异均有统计学意义(P<0.05);空白组与对照组比较差异无统计学意义(P>0.05).结论 空肠弯曲菌甘氨酸提取物28～31 kd外膜蛋白佐剂疫苗能够诱导BALB/c小鼠较好的体液免疫应答和高水平的肠液抗体,将为空肠弯曲菌亚单位疫苗的深入研究奠定重要的实验依据.     

肾癌G250MN／CAIX重组质粒DNA免疫效应的初步研究

【目的】观察PCDNA3．0-G250重组质粒在小鼠中诱导产生DNA免疫应答的特点及抑瘤效应。【方法】PCDNA3．0-G250质粒每隔2周肌注射Balb／c小鼠共3次，对照组同法肌注PCDNA3．0质粒。每次免疫10d后眶后取血，间接免疫荧光法检测血清抗体。6周后取脾细胞观察体外细胞毒效应。同法免疫Balb／c小鼠，第3次免疫时皮下种植sp2／0-PCDNA3．0-G250细胞，观察肿瘤大小及小鼠生存期。【结果】DNA免疫后可检测到特异性抗体，抗体滴度随免疫次数增加逐渐升高，差异有统计学意义（P〈0．01）。体外CTL杀伤率与对照组差别有统计学意义。体内实验发现治疗组与对照组抑瘤效应无明显差异（P〉0．05）。【结论】G250重组质粒DNA免疫能在小鼠中诱导产生特异性细胞免疫及体液免疫，无明显体内抑瘤效应。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.